BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,696,206	Δ1 bp	100%	intergenic (‑134/+91)	RCAP_rcc01564 ← / ← mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,696,206	0	G→.	100.0%	582.8	165	intergenic (‑134/+91)	RCAP_rcc01564/mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): new base (75/88): ref base (1/1): total (76/89)

Only 100 of 172 total aligned reads displayed.


ATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAG  >  NC_014034/1696111‑1696300


aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGGTGTTTGGGCCAAGgggaaac                                                                                          >  1:3961236/1‑97 (MQ=255)


aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAAGgggaaac                                                                                          >  1:336489/1‑97 (MQ=255)


aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                                        <  2:891629/101‑1 (MQ=16)


aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                                        >  1:1397737/1‑101 (MQ=255)


 tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                                       <  1:33938/101‑2 (MQ=255)


  ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                                      >  1:4219327/1‑99 (MQ=12)


   tCGAATCCTTGCACTAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                     <  2:3180335/101‑4 (MQ=12)


   tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                                     >  1:167125/1‑98 (MQ=255)


     gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                   <  2:1704277/101‑6 (MQ=255)


      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  >  1:3626574/1‑95 (MQ=255)


      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  <  2:2664219/101‑7 (MQ=12)


      aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                  <  1:67973/101‑7 (MQ=12)


      aaTCCTTGCACGAATTTTGCAAGGAGGGGAG.AGGGGGGC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGGAGTTTGGGCCAAGgggaaacaccttg                                                                                    >  1:840174/1‑91 (MQ=255)


       aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                                 <  1:4124514/101‑8 (MQ=255)


        tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                                <  2:2846411/101‑9 (MQ=255)


         ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                               <  2:2361167/101‑10 (MQ=9)


         ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGAGGTTTGGGCCAA..GGTGAAacaccttgctt                                                                               >  1:2726372/1‑92 (MQ=255)


          cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                              >  1:3527130/1‑91 (MQ=255)


          cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                              <  1:3125239/101‑11 (MQ=255)


               aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCCATTGACCCCGGGATGTTTGGGCCAA..GGTGAAA.TACCCT.GCTTTTTCtt                                                                       >  2:393602/1‑101 (MQ=255)


               aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                       >  1:516253/1‑101 (MQ=255)


               aCGAATTGTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                       <  2:2022773/101‑1 (MQ=2)


                cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                      <  1:4065673/101‑1 (MQ=255)


                 gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                                     <  1:2983853/101‑1 (MQ=255)


                 gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                                     <  1:3191089/101‑1 (MQ=255)


                 gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTgg                                                                     >  2:3910996/1‑101 (MQ=9)


                  aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGAC.CAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                    <  2:1667877/101‑1 (MQ=255)


                  aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                    <  1:2491666/101‑1 (MQ=9)


                  aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                    >  1:3861251/1‑101 (MQ=255)


                   aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                                   <  1:951987/101‑1 (MQ=255)


                   aTTTTGCAAGGAGGGGAG.AGGGGAGC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCCA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                                   >  2:1797521/1‑101 (MQ=255)


                    ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTAGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                                  >  2:3575452/1‑101 (MQ=9)


                      ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCCATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                                >  1:4403641/1‑101 (MQ=255)


                        gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaaa                                                              >  2:3339231/1‑100 (MQ=255)


                        gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                              >  2:4629246/1‑101 (MQ=255)


                        gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                              <  1:3789785/101‑1 (MQ=9)


                        gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                              <  2:3814493/101‑1 (MQ=9)


                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                             <  1:179839/101‑1 (MQ=255)


                         cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                             <  1:1547415/101‑1 (MQ=255)


                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                            <  1:4078852/101‑1 (MQ=255)


                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                            <  2:101686/101‑1 (MQ=255)


                          aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                            <  2:2166772/101‑1 (MQ=255)


                            ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                          <  1:1076429/101‑1 (MQ=255)


                             gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                         <  1:1213397/101‑1 (MQ=255)


                             gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                         >  1:4162492/1‑101 (MQ=9)


                              aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                        <  1:4253429/101‑1 (MQ=9)


                                gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                      <  2:4413375/101‑1 (MQ=255)


                                      gaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                  <  2:551620/92‑1 (MQ=9)


                                      gaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                  >  1:1675858/10‑101 (MQ=16)


                                       aggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                 <  2:3044577/93‑1 (MQ=255)


                                       aggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                 <  2:1397642/93‑1 (MQ=255)


                                        ggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCTCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                                <  1:913256/94‑1 (MQ=16)


                                        ggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..TCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                                <  2:4364600/94‑1 (MQ=255)


                                        ggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                                <  1:1620916/94‑1 (MQ=255)


                                           gcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                             <  1:2745883/97‑1 (MQ=255)


                                           gcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                             <  1:109706/97‑1 (MQ=255)


                                            cgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                            <  1:2862021/98‑1 (MQ=16)


                                            cgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                            <  2:42925/98‑1 (MQ=16)


                                             gctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                           >  2:306874/3‑101 (MQ=16)


                                                gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                        <  2:3644630/101‑1 (MQ=255)


                                                 ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                       <  1:3760743/101‑1 (MQ=255)


                                                 ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                       >  2:3703069/1‑101 (MQ=19)


                                                 ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                       >  1:2346823/1‑101 (MQ=16)


                                                  cccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                                      <  2:3694153/101‑1 (MQ=16)


                                                   ccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                                     <  2:4433117/101‑1 (MQ=16)


                                                   ccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                                     <  1:1478686/101‑1 (MQ=255)


                                                   ccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                                     >  1:760081/1‑101 (MQ=255)


                                                      cctGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg                                  >  1:2172185/3‑101 (MQ=19)


                                                        tGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                                <  2:2519302/101‑1 (MQ=19)


                                                        tGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                                >  2:383349/1‑101 (MQ=255)


                                                         ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                               <  2:3960951/101‑1 (MQ=19)


                                                           ccTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                             >  2:711831/1‑101 (MQ=255)


                                                           ccTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                             >  1:4637674/1‑101 (MQ=255)


                                                            cTTTCG.GCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                            <  1:1087991/101‑1 (MQ=19)


                                                            cTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                            >  2:1419420/1‑101 (MQ=255)


                                                             tttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg                           >  2:2924605/1‑101 (MQ=255)


                                                              ttCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                          >  2:3247382/1‑101 (MQ=255)


                                                                tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg                         <  2:760006/96‑1 (MQ=19)


                                                                tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg                         <  2:3860966/96‑1 (MQ=19)


                                                                   gccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg                      >  2:4124454/3‑101 (MQ=255)


                                                                     cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc                    >  1:1229759/1‑101 (MQ=255)


                                                                     cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc                    >  2:1008192/1‑101 (MQ=255)


                                                                      aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc                   >  2:2488738/1‑101 (MQ=255)


                                                                        ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc                 >  2:4625903/6‑101 (MQ=255)


                                                                        ttCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc                 <  2:336457/101‑1 (MQ=25)


                                                                           cccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg              <  1:3480643/99‑1 (MQ=25)


                                                                           cccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg              <  2:108978/99‑1 (MQ=25)


                                                                           cACCCCGGGATGTTTGGCCCAA.GGTGAAAC..CCCTTGTTTTTTCTTGGCGAAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt               <  2:1508646/101‑1 (MQ=255)


                                                                            ccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGa             <  1:1367221/100‑1 (MQ=37)


                                                                                gggATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGAGGCCCCCCTGATCGt         >  1:865735/1‑101 (MQ=255)


                                                                                  gatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGc       >  2:1608074/6‑101 (MQ=255)


                                                                                  gatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGc       >  2:717685/6‑101 (MQ=255)


                                                                                   atgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGcc      >  1:3090270/5‑101 (MQ=255)


                                                                                    tgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt     <  1:2557594/98‑1 (MQ=37)


                                                                                     gtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc    >  2:1350637/3‑101 (MQ=255)


                                                                                     gtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc    >  1:4440359/3‑101 (MQ=255)


                                                                                     gtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc    >  1:649042/3‑101 (MQ=255)


                                                                                      tttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa   >  1:4206911/2‑101 (MQ=255)


                                                                                      tttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGAGGCCCCCCTGATCGTGCCTCa   >  1:315777/2‑101 (MQ=37)


                                                                                       ttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg  <  1:3911281/101‑1 (MQ=255)


ATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAG  >  NC_014034/1696111‑1696300

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG