Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,855 Δ1 bp 4.1% intergenic (‑108/+85) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8550G→. 4.1% 15.5 ‑0.8 0.0196intergenic (‑108/+85)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (84/104):  total (89/107)
Fisher's exact test strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

CCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAC  >  NC_014034/1734756‑1734954
                                                                                                     |                                                                                                       
  ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGAc                                                                                                       >  2:1021212/1‑101 (MQ=255)
  ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGAc                                                                                                       <  2:4610887/101‑1 (MQ=255)
  ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGAc                                                                                                       <  2:2230495/101‑1 (MQ=255)
     gAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTcc                                                                                                    >  1:410136/1‑101 (MQ=255)
      aaTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTccc                                                                                                   <  2:4511714/101‑1 (MQ=255)
        tGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTccccc                                                                                                 >  2:1207352/1‑101 (MQ=255)
         ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTcccccc                                                                                                >  1:2553774/1‑101 (MQ=255)
         ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTcccccc                                                                                                <  2:4404005/101‑1 (MQ=255)
          gcgcCTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCg                                                                                               <  1:3933111/101‑1 (MQ=255)
           cgcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCgg                                                                                              >  1:1070417/1‑101 (MQ=255)
            gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCggg                                                                                             <  2:3905654/101‑1 (MQ=255)
             cATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCCGGa                                                                                            >  2:422033/1‑101 (MQ=255)
              aTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGat                                                                                           >  2:910333/1‑101 (MQ=255)
                ccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGatat                                                                                         >  1:808884/1‑101 (MQ=255)
                ccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGatat                                                                                         <  1:2148178/101‑1 (MQ=255)
                  tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATAttt                                                                                       >  2:197665/1‑101 (MQ=255)
                  caggcgcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCCTCCGCCGGCGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATAttt                                                                                       <  2:616689/96‑1 (MQ=18)
                    gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                                     <  1:2365798/101‑1 (MQ=255)
                    gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                                     >  1:416197/1‑101 (MQ=255)
                     cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                                    <  1:4425695/101‑1 (MQ=255)
                     cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                                    <  2:779069/101‑1 (MQ=255)
                     cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                                    >  2:3530466/1‑101 (MQ=255)
                      agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATgg                                                                                   <  2:1078495/101‑1 (MQ=255)
                      agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATgg                                                                                   <  1:117366/101‑1 (MQ=255)
                       gcCGGGGCGGGCAAGCCAAGGGCTGACGCTGGGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGc                                                                                  <  1:2462901/101‑1 (MQ=21)
                       gcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGc                                                                                  <  2:1882044/101‑1 (MQ=255)
                         cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.aa                                                                               <  2:1629925/101‑1 (MQ=255)
                           gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.a                                                                            <  1:2419850/101‑1 (MQ=255)
                           gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.a                                                                            <  1:1627934/101‑1 (MQ=255)
                            ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGG.AAG.At                                                                           >  1:1163249/1‑101 (MQ=255)
                            ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCTCCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.At                                                                           <  2:752858/101‑1 (MQ=255)
                             gcgggcTAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATg                                                                          <  1:765635/101‑1 (MQ=255)
                               gggcAAGCCAAAGGCTGTCGGTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                        <  1:1971221/101‑1 (MQ=18)
                               gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                        <  1:3776779/101‑1 (MQ=255)
                               gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                        <  1:3516714/101‑1 (MQ=255)
                               gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaa                                                                        >  2:1971383/1‑101 (MQ=255)
                                ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaaa                                                                       <  1:3150948/101‑1 (MQ=255)
                                ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGaaa                                                                       >  2:681505/1‑101 (MQ=255)
                                   aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTg                                                                    >  2:3435053/1‑101 (MQ=255)
                                    aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATTTTTATGGC.AAG.ATGAAACTGc                                                                   <  1:2644089/101‑1 (MQ=255)
                                     gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCATGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.g                                                                 <  1:1763434/101‑1 (MQ=255)
                                     gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.g                                                                 <  1:1580250/101‑1 (MQ=255)
                                      ccAATGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.gg                                                                <  2:2930853/101‑1 (MQ=255)
                                        aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGcc                                                              >  2:2059384/1‑101 (MQ=255)
                                           ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCCGGGCAATTATGGC.AAGAATAAAATGGC.GGCCtc                                                            >  1:5823/1‑100 (MQ=17)
                                            gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCa                                                          <  1:2282492/101‑1 (MQ=255)
                                             cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCAt                                                         <  2:2530820/101‑1 (MQ=255)
                                              tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCCTCAtt                                                        >  1:40369/1‑100 (MQ=255)
                                                gcGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                      <  2:3512624/100‑1 (MQ=255)
                                                aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.TTGAAACTGC.GGCCTTCATCtt                                                      <  2:4459524/101‑1 (MQ=255)
                                                aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                      <  1:971746/101‑1 (MQ=255)
                                                aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCtt                                                      <  2:3722210/101‑1 (MQ=255)
                                                 cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTg                                                     >  1:712115/1‑101 (MQ=255)
                                                 cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTg                                                     <  2:2338294/101‑1 (MQ=255)
                                                  gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGc                                                    <  1:3065265/101‑1 (MQ=255)
                                                  gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGc                                                    <  2:1366481/101‑1 (MQ=255)
                                                   cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGcc                                                   >  1:662959/1‑101 (MQ=255)
                                                    tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCTGGATTTTTATGGCTAAC.AGAAAACTCC.GCCCTTCACCtcaca                                                   >  1:3283882/1‑97 (MQ=9)
                                                     gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCAt                                                 <  1:1548212/101‑1 (MQ=255)
                                                      cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGTGAAATTTATGGC.AAG.ATGAAACTGC.GACCTTCATCTTGCCATa                                                >  1:436418/1‑101 (MQ=17)
                                                      cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                <  2:3397376/101‑1 (MQ=255)
                                                      cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                >  1:474469/1‑101 (MQ=255)
                                                       gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATaa                                               >  2:1097034/1‑101 (MQ=255)
                                                         cAGCCGTCGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                             <  2:3681062/101‑1 (MQ=255)
                                                         cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                             <  1:3849642/101‑1 (MQ=255)
                                                         cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                             <  2:4051915/101‑1 (MQ=255)
                                                          aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                            >  2:1428254/1‑101 (MQ=255)
                                                          aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                            >  2:2922739/1‑101 (MQ=255)
                                                              gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTCCATCTTGCCATAAATATccc                                        >  1:295962/1‑101 (MQ=255)
                                                               gcgggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                      <  2:557275/100‑1 (MQ=255)
                                                               aCGGGCAGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                      <  1:1657182/101‑1 (MQ=255)
                                                                cgggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                     >  1:2105841/1‑101 (MQ=255)
                                                                  ggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATACC.gggg                                   >  2:1448584/1‑101 (MQ=255)
                                                                   tgacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                 >  1:2761741/9‑101 (MQ=2)
                                                                   tgacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                 >  2:18019/9‑101 (MQ=2)
                                                                   gccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                  <  2:3253867/101‑1 (MQ=255)
                                                                   gccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                  <  2:416160/101‑1 (MQ=255)
                                                                    ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGG.AAG.ATGAAACTGC.GGGCTTCATCTTGCCCTAAATATCCC.cggggg                                 >  1:1745823/1‑99 (MQ=14)
                                                                    ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                 <  2:3784874/101‑1 (MQ=255)
                                                                    ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                 <  1:1378546/101‑1 (MQ=255)
                                                                     acggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                               >  2:4309734/7‑99 (MQ=2)
                                                                     acggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                               >  1:4440965/7‑99 (MQ=2)
                                                                     acggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                               >  2:923127/7‑99 (MQ=2)
                                                                      ggggAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                               <  2:3648148/101‑1 (MQ=255)
                                                                        gcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                             <  2:3891332/101‑1 (MQ=255)
                                                                        gacggggggCGCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGC.AAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaattg                            <  1:3012753/98‑6 (MQ=9)
                                                                         cAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                            >  1:4273313/1‑101 (MQ=255)
                                                                           ggggggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                          <  2:1070328/101‑1 (MQ=255)
                                                                                  cctccccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAACCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                   <  1:2334251/96‑1 (MQ=255)
                                                                                   ctgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                  <  1:1207444/97‑1 (MQ=255)
                                                                                    tgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.GTGAAAATGC.GGCCTTCATCTTTCCATAAATATCCC.GGGGTGCGGGGGAGAGCCCCGc                 >  1:1105222/4‑101 (MQ=18)
                                                                                     gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                <  1:1021295/99‑1 (MQ=255)
                                                                                        cc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa             >  2:4560387/1‑101 (MQ=255)
                                                                                         c.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc            >  2:159375/1‑101 (MQ=255)
                                                                                           ccGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc           >  1:3580067/1‑101 (MQ=255)
                                                                                               ccTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg       >  2:2004868/1‑101 (MQ=255)
                                                                                                cTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa      >  2:2702641/1‑101 (MQ=255)
                                                                                                cTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa      <  1:1192006/101‑1 (MQ=255)
                                                                                                 tGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt     <  2:2105691/101‑1 (MQ=255)
                                                                                                 tGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt     <  2:1745704/101‑1 (MQ=255)
                                                                                                     |                                                                                                       
CCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGC.AAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAC  >  NC_014034/1734756‑1734954

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG