Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,856 Δ1 bp 4.1% intergenic (‑109/+84) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8560A→. 4.1% 15.5 ‑0.7 ‑0.1197intergenic (‑109/+84)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (85/102):  total (90/107)
Fisher's exact test strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.37e-01

Only 100 of 204 total aligned reads displayed.

GGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAA  >  NC_014034/1734758‑1734956
                                                                                                     |                                                                                                       
ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGAc                                                                                                        <  2:4610887/101‑1 (MQ=255)
ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGAc                                                                                                        <  2:2230495/101‑1 (MQ=255)
 gggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACt                                                                                                       >  1:2346611/1‑101 (MQ=255)
    aaTGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.ccc                                                                                                   <  2:4511714/101‑1 (MQ=255)
      tGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.ccccc                                                                                                 >  2:1207352/1‑101 (MQ=255)
       ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.cccccc                                                                                                <  2:4404005/101‑1 (MQ=255)
        gcgcCTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCg                                                                                               <  1:3933111/101‑1 (MQ=255)
         cgcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCgg                                                                                              >  1:1070417/1‑101 (MQ=255)
          gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCggg                                                                                             <  2:3905654/101‑1 (MQ=255)
           cATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCCGGa                                                                                            >  2:422033/1‑101 (MQ=255)
            aTCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGat                                                                                           >  2:910333/1‑101 (MQ=255)
             tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGata                                                                                          <  1:3574375/101‑1 (MQ=255)
             tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGata                                                                                          >  1:80430/1‑101 (MQ=255)
                tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGGTAttt                                                                                       >  2:3520758/1‑101 (MQ=255)
                tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATAttt                                                                                       >  2:197665/1‑101 (MQ=255)
                tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATAttt                                                                                       <  2:3580478/101‑1 (MQ=255)
                caggcgcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCCTCCGCCGGCGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATAttt                                                                                       <  2:616689/96‑1 (MQ=18)
                   cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATg                                                                                    <  2:779069/101‑1 (MQ=255)
                    agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATgg                                                                                   <  1:117366/101‑1 (MQ=255)
                    agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATgg                                                                                   <  2:1078495/101‑1 (MQ=255)
                     gcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGc                                                                                  <  2:1882044/101‑1 (MQ=255)
                       cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCaa                                                                                <  2:1629925/101‑1 (MQ=255)
                        ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAg                                                                               >  1:2277184/1‑101 (MQ=255)
                        ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAg                                                                               >  1:4111430/1‑101 (MQ=255)
                         gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGa                                                                              <  1:2761632/101‑1 (MQ=255)
                         gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGa                                                                              <  1:1627934/101‑1 (MQ=255)
                          ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGGAAGAt                                                                             >  1:1163249/1‑101 (MQ=255)
                          ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGAt                                                                             >  1:4334206/1‑101 (MQ=255)
                          ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCTCCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGAt                                                                             <  2:752858/101‑1 (MQ=255)
                           gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATg                                                                            <  1:3516638/101‑1 (MQ=255)
                           gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCCGGCTATTTTTTGGAAGATg                                                                            >  1:56399/1‑101 (MQ=21)
                            cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGa                                                                           <  1:2511831/101‑1 (MQ=255)
                             gggcAAGCCAAAGGCTGTCGGTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGaa                                                                          <  1:1971221/101‑1 (MQ=18)
                             gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGaa                                                                          >  2:1971383/1‑101 (MQ=255)
                              ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGaaa                                                                         <  1:3150948/101‑1 (MQ=255)
                              ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGaaa                                                                         >  2:681505/1‑101 (MQ=255)
                                  aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGc                                                                     <  1:3790451/101‑1 (MQ=255)
                                   gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCATGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                   <  1:1763434/101‑1 (MQ=255)
                                   gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                   <  1:1580250/101‑1 (MQ=255)
                                    ccAATGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                                                  <  2:2930853/101‑1 (MQ=255)
                                    ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                                                  >  1:66097/1‑101 (MQ=255)
                                      aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                >  1:4395081/1‑101 (MQ=255)
                                           cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                           <  2:2530820/101‑1 (MQ=255)
                                            tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCCGGGAAATTTTTGGCAAAAAGAAAACGC.GGCCTTCAt                                                           >  1:2399213/1‑101 (MQ=17)
                                             gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                         <  1:732751/101‑1 (MQ=255)
                                             gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAAAAGAAACTGC.GGGCTTCATCt                                                         >  1:2616302/1‑101 (MQ=18)
                                              aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTCC.CCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                        <  1:3938117/101‑1 (MQ=255)
                                              aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGTTGAAACTGC.GGCCTTCATCtt                                                        <  2:4459524/101‑1 (MQ=255)
                                              aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                        <  2:3722210/101‑1 (MQ=255)
                                               cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                       <  2:2338294/101‑1 (MQ=255)
                                               cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                       <  1:4629208/101‑1 (MQ=255)
                                                gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                      <  2:1366481/101‑1 (MQ=255)
                                                gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                      <  1:3065265/101‑1 (MQ=255)
                                                   gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                   <  1:1548212/101‑1 (MQ=255)
                                                    cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                  >  1:2294734/1‑101 (MQ=255)
                                                    cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                  <  2:3397376/101‑1 (MQ=255)
                                                     gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                 >  2:1097034/1‑101 (MQ=255)
                                                     gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                 <  1:4223937/101‑1 (MQ=255)
                                                      tCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                >  1:3719863/1‑101 (MQ=255)
                                                       cAGCCGTCGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                               <  2:3681062/101‑1 (MQ=255)
                                                       cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                               <  2:4051915/101‑1 (MQ=255)
                                                        aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                              >  2:1428254/1‑101 (MQ=255)
                                                        aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                              >  2:2922739/1‑101 (MQ=255)
                                                            gACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTCCATCTTGCCATAAATATccc                                          >  1:295962/1‑101 (MQ=255)
                                                             gcgggccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                        <  2:557275/100‑1 (MQ=255)
                                                             aCGGGCAGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                        <  1:1657182/101‑1 (MQ=255)
                                                               gggccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATTCC.ggg                                      >  2:35097/1‑101 (MQ=255)
                                                                ggccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATACC.gggg                                     >  2:1448584/1‑101 (MQ=255)
                                                                 tgacggacggggggCTCT.G.CCC.CCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                   >  2:18019/9‑101 (MQ=2)
                                                                 gccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                    <  2:3253867/101‑1 (MQ=255)
                                                                 gccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                    <  2:416160/101‑1 (MQ=255)
                                                                 gccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                    <  1:848624/101‑1 (MQ=255)
                                                                 gccgggcAGGAGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                    <  2:2077344/101‑1 (MQ=255)
                                                                  ccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGGAAGATGAAACTGC.GGGCTTCATCTTGCCCTAAATATCCC.cggggg                                   >  1:1745823/1‑99 (MQ=14)
                                                                  ccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                   <  1:1378546/101‑1 (MQ=255)
                                                                  ccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                   <  2:3784874/101‑1 (MQ=255)
                                                                   cgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                  >  1:663736/1‑101 (MQ=255)
                                                                   acggacggggggCTCT.G.CCC.CCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                 >  2:4309734/7‑99 (MQ=2)
                                                                   acggacggggggCTCT.G.CCC.CCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                 >  2:923127/7‑99 (MQ=2)
                                                                    ggggAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                 <  2:3648148/101‑1 (MQ=255)
                                                                    gggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                 >  2:2106101/1‑101 (MQ=255)
                                                                    gggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                 <  1:3864913/101‑1 (MQ=255)
                                                                      gcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                               <  2:3891332/101‑1 (MQ=255)
                                                                      gacggggggCGCT.G.CCC.CCCGGCTTCG..CT.CCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaattg                              <  1:3012753/98‑6 (MQ=9)
                                                                         ggggggCGCT.GCCCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                            <  2:1070328/101‑1 (MQ=255)
                                                                                  ctgcccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                    <  1:1207444/97‑1 (MQ=255)
                                                                                   tgcccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGGTGAAAATGC.GGCCTTCATCTTTCCATAAATATCCC.GGGGTGCGGGGGAGAGCCCCGc                   >  1:1105222/4‑101 (MQ=18)
                                                                                   tgcccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                   >  1:4439373/4‑101 (MQ=255)
                                                                                    gcccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                  <  1:1021295/99‑1 (MQ=255)
                                                                                     cccc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                 >  1:2136297/2‑101 (MQ=255)
                                                                                       cc.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa               >  2:4560387/1‑101 (MQ=255)
                                                                                       cc.ccGGCCTGCGGGCT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa               <  1:2431716/101‑1 (MQ=255)
                                                                                        c.ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc              >  2:159375/1‑101 (MQ=255)
                                                                                          ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc             <  1:4078692/101‑1 (MQ=255)
                                                                                          ccGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAAcc             >  2:1020723/1‑101 (MQ=255)
                                                                                              ccTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg         >  2:2004868/1‑101 (MQ=255)
                                                                                               cTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa        >  2:2702641/1‑101 (MQ=255)
                                                                                               cTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa        <  1:1192006/101‑1 (MQ=255)
                                                                                                tGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt       <  2:1745704/101‑1 (MQ=255)
                                                                                                     aCT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa  <  1:2077801/101‑1 (MQ=255)
                                                                                                     |                                                                                                       
GGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCC.CCGTCCTGCGGACT.CCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAA  >  NC_014034/1734758‑1734956

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG