Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,786,540 Δ1 bp 97.4% coding (674/819 nt) RCAP_rcc01648 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,786,5400C→.97.4% 8.7 0.0 ‑0.5192coding (674/819 nt)RCAP_rcc01648ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (89/98):  ref base (2/3):  total (91/101)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.08e-01

Only 100 of 198 total aligned reads displayed.

AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636
                                                                                                     |                                                                                                
aGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGc                                                                                                  >  2:2822026/1‑101 (MQ=255)
 gCTGATGCCGCATATGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGGCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGct                                                                                                 <  1:950175/101‑2 (MQ=255)
  cTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGctt                                                                                                <  1:4568362/101‑3 (MQ=255)
   tgagggcGCATCTGGTCGCCGCGGCGCGGGCGCGGATTTCTCTGATCTGGAAGGACGTGCTGGAGGTCGAATTTATAGG.CCGCTCGAACGGGATCGGCTtt                                                                                               >  2:4599558/7‑101 (MQ=255)
   tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGCTCTGGATGGTCGTGCTGGGGGTCGAATTTCTGGG.CGGCTCGAACGGGATGGGCTtt                                                                                               >  1:3874596/1‑101 (MQ=255)
    gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTttc                                                                                              <  1:400991/101‑2 (MQ=255)
     aTGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCg                                                                                            >  2:1589989/1‑101 (MQ=255)
      tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  2:877213/101‑1 (MQ=255)
      tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  1:574982/101‑1 (MQ=255)
       gCCGCAGCTGATCGCCGCGGCGGGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTa                                                                                          <  1:2497725/101‑1 (MQ=255)
        ccGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                         <  1:4348441/101‑1 (MQ=255)
         cGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATc                                                                                        >  2:3613631/1‑101 (MQ=255)
         cGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATc                                                                                        >  1:2864958/1‑101 (MQ=255)
          gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                       >  2:1386165/1‑101 (MQ=255)
          gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                       <  1:1302096/101‑1 (MQ=255)
           cATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCa                                                                                      >  1:3538477/1‑101 (MQ=255)
            aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                     <  2:3495452/101‑1 (MQ=255)
               tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                  <  2:147576/101‑1 (MQ=255)
                gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 >  1:2105085/1‑101 (MQ=255)
                 aTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGaa                                                                                <  2:587309/101‑1 (MQ=255)
                  tCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAt                                                                               <  1:1186362/101‑1 (MQ=255)
                   cgccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.GTTTCGTATCCATCTGAAtt                                                                              >  2:1648624/1‑101 (MQ=255)
                   cgccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAtt                                                                              >  1:1655626/1‑101 (MQ=255)
                    gccgcGGCGCGGGCGCGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTGCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                             <  2:3138788/101‑1 (MQ=255)
                    cccgcTGCGCGGGCGTGTCTTTCGCTGTTCTCGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                             <  1:2196643/100‑1 (MQ=255)
                     ccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTc                                                                            <  2:3978275/101‑1 (MQ=255)
                       gcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCa                                                                          <  1:4191734/101‑1 (MQ=255)
                         ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                        >  1:111503/1‑101 (MQ=255)
                            gcgggcggGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGt                                                                     >  2:3051689/1‑101 (MQ=255)
                              gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   <  2:2104935/101‑1 (MQ=255)
                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  1:618813/101‑1 (MQ=255)
                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  2:2864742/101‑1 (MQ=255)
                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  >  1:1802077/1‑101 (MQ=255)
                               ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  1:4036129/101‑1 (MQ=255)
                                gcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGa                                                                 <  2:126433/101‑1 (MQ=255)
                                gcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGa                                                                 >  1:1549023/1‑101 (MQ=255)
                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  2:4111368/95‑1 (MQ=255)
                                 cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                >  2:2138959/7‑101 (MQ=255)
                                   gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                              >  1:330371/5‑101 (MQ=255)
                                   gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                              >  2:115600/5‑101 (MQ=255)
                                     gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                            <  1:4420274/99‑1 (MQ=255)
                                     gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                            <  1:2328813/99‑1 (MQ=255)
                                      gTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                           <  1:510339/101‑1 (MQ=255)
                                      ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                           <  2:150242/100‑1 (MQ=255)
                                      ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                           <  1:1634360/100‑1 (MQ=255)
                                       tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          >  1:1714768/1‑101 (MQ=255)
                                       tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          <  1:1224985/101‑1 (MQ=255)
                                        ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                         <  2:2024639/101‑1 (MQ=255)
                                          cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                       >  2:4576669/1‑101 (MQ=255)
                                             tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                    >  1:1241349/1‑101 (MQ=255)
                                              gATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGc                                                   >  2:1101810/1‑101 (MQ=255)
                                               aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCTACATTGCCGAGGTGCt                                                  >  1:1820170/1‑101 (MQ=255)
                                               aTCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCt                                                  <  2:2619572/101‑1 (MQ=255)
                                                tCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 <  2:1864809/101‑1 (MQ=255)
                                                tCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GGCCCGCTCGAACGGGTTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 <  1:1457533/101‑1 (MQ=255)
                                                  tGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGc                                               >  1:2656795/1‑101 (MQ=255)
                                                    gAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCt                                             <  2:2377991/101‑1 (MQ=255)
                                                     aaGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                            <  1:223133/101‑1 (MQ=255)
                                                           gTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  1:4447034/1‑101 (MQ=255)
                                                           gTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      <  1:1648536/101‑1 (MQ=255)
                                                            tGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGATGCTCGCCTGggcggcg                                     >  2:4186090/1‑101 (MQ=255)
                                                             gCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcgg                                    >  2:2219407/1‑101 (MQ=255)
                                                                ggtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                 <  2:585493/101‑1 (MQ=255)
                                                                ggtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                 <  1:381173/101‑1 (MQ=255)
                                                                ggtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                 <  1:1268086/101‑1 (MQ=255)
                                                                 gtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCttt                                <  2:1801958/101‑1 (MQ=255)
                                                                  tggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                               <  2:3225108/101‑1 (MQ=255)
                                                                  tgctCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTc                               <  2:761130/98‑1 (MQ=255)
                                                                     tCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTc                            <  2:3302691/101‑1 (MQ=255)
                                                                      cGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                           <  1:3051933/101‑1 (MQ=255)
                                                                       gAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                          <  1:1743287/101‑1 (MQ=255)
                                                                       gAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                          >  1:1845854/1‑101 (MQ=255)
                                                                       gAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTg                          >  2:4264765/1‑101 (MQ=255)
                                                                        taTTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                         <  1:3448893/100‑1 (MQ=255)
                                                                          tttCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                       >  1:989470/1‑101 (MQ=255)
                                                                          tttCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                       >  2:1767508/1‑101 (MQ=255)
                                                                           ttctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                      <  1:1344271/96‑1 (MQ=255)
                                                                           ttctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                      >  1:1597709/6‑101 (MQ=255)
                                                                            tctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                     >  2:2649542/5‑101 (MQ=255)
                                                                            tctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGa                     >  1:1032025/5‑101 (MQ=255)
                                                                             ctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    <  2:511695/98‑1 (MQ=255)
                                                                              tggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   >  2:127397/3‑101 (MQ=255)
                                                                              tggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   >  1:4660877/3‑101 (MQ=255)
                                                                              tggg.CCGCTCGAACGGGATCGG.CTTTCGTAACCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   >  1:4151675/3‑101 (MQ=255)
                                                                                gg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                 >  2:1906459/1‑101 (MQ=255)
                                                                                 g.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                <  1:1981103/101‑1 (MQ=255)
                                                                                 g.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                <  2:1900673/101‑1 (MQ=255)
                                                                                        cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc          <  2:3694036/101‑1 (MQ=255)
                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  2:385410/1‑101 (MQ=255)
                                                                                         gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         <  2:1214171/101‑1 (MQ=255)
                                                                                          aaCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGa        <  1:444097/101‑1 (MQ=255)
                                                                                           aCGGGATCGG.CTTTCGTACCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGaa       <  2:3399580/101‑1 (MQ=255)
                                                                                            cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt      <  2:3531467/101‑1 (MQ=255)
                                                                                             gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg     >  2:1663420/1‑101 (MQ=255)
                                                                                             gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg     >  1:2179556/1‑101 (MQ=255)
                                                                                             gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg     <  1:1008943/101‑1 (MQ=255)
                                                                                               gATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGc   >  2:2768238/1‑101 (MQ=255)
                                                                                               gATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGc   <  1:2035303/101‑1 (MQ=255)
                                                                                                aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt  >  2:3780080/1‑101 (MQ=255)
                                                                                                aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt  >  1:3713613/1‑101 (MQ=255)
                                                                                                     |                                                                                                
AGCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786441‑1786636

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG