Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,355 Δ1 bp 100% intergenic (‑48/+7) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3550C→.100.0% 880.9 198intergenic (‑48/+7)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (102/96):  ref base (0/0):  total (102/96)

Only 100 of 222 total aligned reads displayed.

TCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGC.GGGGTG.T.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCGCGAT  >  NC_014034/1974258‑1974450
                                                                                                      |                                                                                               
tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACTGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCAc                                                                                                 <  1:3856391/101‑1 (MQ=255)
tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCAc                                                                                                 <  1:1744122/101‑1 (MQ=255)
 cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCAcg                                                                                                <  2:2837011/101‑2 (MQ=255)
 cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGG.TCC.CCCCGCCCGGATAGCAcg                                                                                                >  2:1512951/1‑100 (MQ=255)
   gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGCCGGTCTCCTGGGGGGTTGGGGC.GGGGGGCG.CTCCCCGCCCTGATAGCACGgg                                                                                              >  2:1087133/1‑101 (MQ=255)
      gAAAGGATCGAGGGGGCGATCTTCATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGtt                                                                                          >  1:4213463/1‑101 (MQ=255)
       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCCCCCGGATAGCA.CGGCGTTc                                                                                         >  1:2283156/1‑101 (MQ=255)
        aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                                                        <  2:161613/101‑1 (MQ=255)
         aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAg                                                                                       >  1:3016589/1‑101 (MQ=255)
          ggATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGt                                                                                      <  2:4589339/101‑1 (MQ=255)
           gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                                                     <  1:112811/101‑1 (MQ=255)
           gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                                                     >  1:1239593/1‑101 (MQ=255)
             tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                                                   <  2:2197289/101‑3 (MQ=255)
              cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                                  >  2:1579967/1‑101 (MQ=255)
              cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                                  >  1:2081561/1‑101 (MQ=255)
              cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                                  <  1:2559514/101‑1 (MQ=255)
               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                                 >  1:4052818/1‑101 (MQ=255)
               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                                 >  1:1941255/1‑101 (MQ=255)
               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGGTCAGTTCACg                                                                                 >  2:4317797/1‑101 (MQ=255)
               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGGTCACg                                                                                 >  1:3710824/1‑101 (MQ=255)
                 gggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGcc                                                                               >  1:717946/1‑101 (MQ=255)
                  ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                                                              <  2:4362350/101‑1 (MQ=255)
                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                                             <  2:2487620/101‑1 (MQ=255)
                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                                            <  2:1862261/101‑1 (MQ=255)
                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                                            >  1:1415852/1‑101 (MQ=255)
                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCcga                                                                           >  1:1000408/1‑101 (MQ=255)
                      cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                                          <  2:3497951/101‑1 (MQ=255)
                       gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                                         <  2:2567924/101‑1 (MQ=255)
                       gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                                         <  1:1526286/101‑1 (MQ=255)
                         tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCCGAGCGa                                                                       >  2:1794570/1‑101 (MQ=255)
                         tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGa                                                                       >  1:283764/1‑101 (MQ=255)
                         tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGa                                                                       <  2:3672559/101‑1 (MQ=255)
                          cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                                      >  2:3919750/1‑101 (MQ=255)
                          cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGCG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                                      >  1:1035408/1‑101 (MQ=255)
                           ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                                     <  2:2681593/101‑1 (MQ=255)
                           ttGAAGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                                     <  1:3317660/101‑1 (MQ=255)
                            tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTT.TGC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                                                    >  2:2992314/1‑101 (MQ=255)
                            tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                                                    <  2:870299/101‑1 (MQ=255)
                             gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                                   <  2:2707430/101‑1 (MQ=255)
                              aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                                                  >  2:4431205/1‑101 (MQ=255)
                              aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                                                  >  1:1306660/1‑101 (MQ=255)
                                gtaaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                                                >  1:703216/3‑101 (MQ=255)
                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                                                <  2:2161070/101‑1 (MQ=255)
                                   aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCCCCCGGATAGCA.CGACGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                                                             >  1:4010499/1‑101 (MQ=255)
                                    cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                                            >  2:4458741/1‑101 (MQ=255)
                                      gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                                          <  1:2910539/101‑1 (MQ=255)
                                        cGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                                        <  1:4342046/101‑1 (MQ=255)
                                        cGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                                        <  1:363731/101‑1 (MQ=255)
                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                                       >  2:3752929/1‑101 (MQ=255)
                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                                       <  1:2223692/101‑1 (MQ=255)
                                           aGGTCATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATaga                                                     <  2:704450/101‑1 (MQ=255)
                                               cATGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                                 >  2:245798/1‑101 (MQ=255)
                                                aTGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                                                >  2:1180762/1‑101 (MQ=255)
                                                aTGTCGGTCTCCTGAGGGGTTGGGGCGGGGGGG.TCC.CCCCCCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                                                >  1:581862/1‑101 (MQ=255)
                                                 tGTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGt                                               >  2:2225919/1‑101 (MQ=255)
                                                  gTCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTc                                              <  2:1904293/101‑1 (MQ=255)
                                                   tCGGTCTCCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                                             <  1:32291/101‑1 (MQ=255)
                                                        ctcCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                                        >  2:1705518/1‑101 (MQ=255)
                                                        ctcCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                                        <  2:3577261/101‑1 (MQ=255)
                                                        ctcCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                                        <  1:1603330/101‑1 (MQ=255)
                                                         tcCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                                       >  2:1547906/1‑101 (MQ=255)
                                                         tcCTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                                       >  1:21290/1‑101 (MQ=255)
                                                           cTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACTCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                                     <  2:3016360/101‑1 (MQ=255)
                                                           cTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                                     <  2:2117881/101‑1 (MQ=255)
                                                           cTGAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                                     >  2:2264117/1‑101 (MQ=255)
                                                             gAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                                   >  2:3132383/1‑101 (MQ=255)
                                                             gAGGGGTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                                   >  1:3044598/1‑101 (MQ=255)
                                                               ggggTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg                                 >  2:4476240/1‑101 (MQ=255)
                                                               ggggTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg                                 <  2:196685/101‑1 (MQ=255)
                                                               ggggTTGGGGCGGGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg                                 <  2:756398/101‑1 (MQ=255)
                                                                    tggggcgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                            <  2:4107056/94‑1 (MQ=255)
                                                                    tggggcgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                            >  2:238057/8‑101 (MQ=255)
                                                                    tggggcgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                            >  2:2327528/8‑101 (MQ=255)
                                                                    tggggcgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                            >  1:1465107/8‑101 (MQ=255)
                                                                     ggggcgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt                           >  2:830257/7‑101 (MQ=255)
                                                                     ggggcgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt                           >  1:2805229/7‑101 (MQ=255)
                                                                       ggcgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc                         >  2:1125482/5‑101 (MQ=255)
                                                                        gcgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCg                        <  1:3885307/98‑1 (MQ=255)
                                                                         cgggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa                       <  1:1705629/99‑1 (MQ=255)
                                                                          gggggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa                      >  1:3628264/2‑101 (MQ=255)
                                                                            gggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACg                    >  2:3885860/1‑101 (MQ=255)
                                                                             ggTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGc                   >  2:3997634/1‑101 (MQ=255)
                                                                                 gtcc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCg                >  1:3128448/4‑101 (MQ=255)
                                                                                 gtcc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCg                <  1:4107049/98‑1 (MQ=255)
                                                                                   cc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCggg              >  2:3056131/2‑101 (MQ=255)
                                                                                   cc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCggg              <  1:3464272/100‑1 (MQ=255)
                                                                                   cc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCggg              <  2:418067/100‑1 (MQ=255)
                                                                                    c.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGc             >  1:1342174/1‑101 (MQ=255)
                                                                                      ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGcc            >  1:2905876/1‑101 (MQ=255)
                                                                                      ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGcc            <  2:1423637/101‑1 (MQ=255)
                                                                                      ccccGCCCGAATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGcc            <  1:1085814/101‑1 (MQ=255)
                                                                                       cccgcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCa           >  2:456174/1‑101 (MQ=255)
                                                                                         cgcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGt         <  2:4052532/101‑1 (MQ=255)
                                                                                         cgcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGt         <  1:793559/101‑1 (MQ=255)
                                                                                          gcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGtt        <  2:260452/101‑1 (MQ=255)
                                                                                           cccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTc       <  1:497223/101‑1 (MQ=255)
                                                                                           cccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTc       <  1:4431497/101‑1 (MQ=255)
                                                                                             cgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTcgc     <  1:4312122/101‑1 (MQ=255)
                                                                                              ggATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTcgcg    >  2:2948185/1‑101 (MQ=255)
                                                                                                aTAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCgcgat  <  1:4523024/101‑1 (MQ=255)
                                                                                                      |                                                                                               
TCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGC.GGGGTG.T.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCGCGAT  >  NC_014034/1974258‑1974450

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG