Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,040,373 Δ1 bp 95.0% intergenic (‑121/‑267) RCAP_rcc01888 ← / → RCAP_rcc01889 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,040,3730C→.95.0% 30.0 ‑2.1 ‑0.8218intergenic (‑121/‑267)RCAP_rcc01888/RCAP_rcc01889hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (110/97):  ref base (3/8):  total (113/105)
Fisher's exact test strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.54e-01

Only 100 of 226 total aligned reads displayed.

GGCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.CGCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCA  >  NC_014034/2040272‑2040470
                                                                                                     |                                                                                                  
  cACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCAccg                                                                                                  >  2:2803365/1‑100 (MQ=255)
    ccGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgg                                                                                                <  2:141531/101‑1 (MQ=255)
    ccGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgg                                                                                                <  1:2352901/101‑1 (MQ=255)
    ccGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgg                                                                                                <  2:2285347/101‑1 (MQ=255)
     cGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGggg                                                                                               >  2:1132692/1‑101 (MQ=255)
     cGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGggg                                                                                               <  1:2109000/101‑1 (MQ=255)
      ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                              <  2:2513674/101‑2 (MQ=255)
      ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                              <  2:3962065/101‑2 (MQ=255)
          tctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGAt                                                                                         <  1:2766637/101‑1 (MQ=255)
          tctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGAt                                                                                         <  1:822349/101‑1 (MQ=255)
          tctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGAt                                                                                         >  2:1196195/1‑101 (MQ=255)
           ctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATg                                                                                        >  2:240632/1‑101 (MQ=255)
            tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGAttg                                                                                       >  2:4647549/1‑99 (MQ=255)
            tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                       >  2:1911498/1‑101 (MQ=255)
            tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                       <  2:3264170/101‑1 (MQ=255)
             gggTTGGGATTGAAAAAAACCGGACCGGCCAGGGGCCGGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                      <  1:321974/101‑1 (MQ=255)
             gggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                      >  1:2222200/1‑101 (MQ=255)
             gggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                      >  1:872245/1‑101 (MQ=255)
             gggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                      >  2:1575208/1‑101 (MQ=255)
              ggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCt                                                                                     >  2:3495062/1‑101 (MQ=255)
               gTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCtt                                                                                    <  1:1798445/101‑1 (MQ=255)
                 tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                  <  1:2173508/101‑1 (MQ=255)
                  gggATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcg                                                                                 <  2:1373098/101‑1 (MQ=255)
                     aTGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCgg                                                                              >  1:143255/1‑101 (MQ=255)
                      tGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGt                                                                             <  1:4463994/101‑1 (MQ=255)
                      tGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGt                                                                             <  1:1436558/101‑1 (MQ=255)
                        gAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTAt                                                                           >  1:2039831/1‑101 (MQ=255)
                         aaaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATg                                                                          <  2:4587698/101‑1 (MQ=255)
                         aaaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATg                                                                          >  1:3640039/1‑101 (MQ=255)
                          aaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATgg                                                                         <  1:2586130/101‑1 (MQ=255)
                          aaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATgg                                                                         <  2:58560/101‑1 (MQ=255)
                               aCCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGt                                                                    >  1:1384313/1‑101 (MQ=255)
                                 cGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGc                                                                  >  1:2750084/1‑101 (MQ=255)
                                  ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGGGcc                                                                 >  1:1852218/1‑101 (MQ=255)
                                    aaCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaa                                                               >  2:1976288/1‑101 (MQ=255)
                                     aCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaaa                                                              <  1:3398057/101‑1 (MQ=255)
                                     aCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaaa                                                              <  1:3125263/101‑1 (MQ=255)
                                     aCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaaa                                                              <  1:4045580/101‑1 (MQ=255)
                                     aCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaaa                                                              >  1:1990396/1‑101 (MQ=255)
                                        gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                           <  2:1994273/101‑1 (MQ=255)
                                        gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                           <  2:1273864/101‑1 (MQ=255)
                                        gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                           >  1:914281/1‑101 (MQ=255)
                                         ccAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                          <  1:2913220/101‑1 (MQ=255)
                                         ccAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                          >  2:3735739/1‑101 (MQ=255)
                                          cAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcgg                                                         >  2:2765057/1‑101 (MQ=255)
                                           aGGGGCCAGACGCAGGCGT.GGGGGATCCGGTGGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggc                                                        >  1:4157902/1‑101 (MQ=255)
                                           aGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggc                                                        >  2:346299/1‑101 (MQ=255)
                                           aGGGGCCAGACGCAGGCGT.GGGGGATCCGGTAGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggc                                                        <  1:3452436/101‑1 (MQ=255)
                                               gCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGcc                                                    <  1:1196287/101‑1 (MQ=255)
                                               gCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGcc                                                    >  1:241507/1‑101 (MQ=255)
                                                ccAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGAGCGTGCCAAAGACGGCGGCCg                                                   <  1:2640413/101‑1 (MQ=255)
                                                  aGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACggcggcgggt                                                 <  2:2190579/101‑5 (MQ=255)
                                                  aGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCa                                                 >  2:4283598/1‑101 (MQ=255)
                                                  aGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCa                                                 >  2:4530837/1‑101 (MQ=255)
                                                   gACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaa                                                <  1:4556809/101‑1 (MQ=255)
                                                    aCGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaaa                                               <  1:4531132/101‑1 (MQ=255)
                                                    aCGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaaa                                               >  2:178611/1‑101 (MQ=255)
                                                      gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAgg                                             >  1:1054889/1‑101 (MQ=255)
                                                        cggCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.cc                                          <  2:2088483/100‑2 (MQ=255)
                                                        aGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.cc                                          <  1:4600948/101‑2 (MQ=255)
                                                        aGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.cc                                          >  2:3069957/1‑100 (MQ=255)
                                                            cgtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgcg                                       >  1:1103782/4‑97 (MQ=255)
                                                            cgtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgcg                                       >  2:1984848/4‑97 (MQ=255)
                                                             gtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgcgc                                      >  1:2166633/3‑96 (MQ=255)
                                                                ggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCAt                                    <  1:3509933/101‑1 (MQ=255)
                                                                 gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATa                                   <  2:793298/101‑1 (MQ=255)
                                                                 gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATa                                   <  1:3079520/101‑1 (MQ=255)
                                                                  ggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATaa                                  >  2:752739/1‑101 (MQ=255)
                                                                     tCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTg                               <  1:730676/101‑1 (MQ=255)
                                                                      ccGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGa                              <  2:4446582/101‑1 (MQ=255)
                                                                        ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGc                            <  1:1667865/101‑1 (MQ=255)
                                                                        ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGc                            >  2:3138872/1‑101 (MQ=255)
                                                                         gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                           <  1:1901553/101‑1 (MQ=255)
                                                                         gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGcc                           <  1:3168361/101‑1 (MQ=255)
                                                                           cGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCAGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCa                         <  1:3972597/101‑1 (MQ=255)
                                                                            gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAc                        >  2:489047/1‑101 (MQ=255)
                                                                            gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGAGCCATAAGTGAGCCCAc                        >  2:4192045/1‑101 (MQ=255)
                                                                             aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAcc                       >  1:1887946/1‑101 (MQ=255)
                                                                             aaGGTCAGACGTGGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAcc                       >  1:2533266/1‑101 (MQ=255)
                                                                              aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAccg                      >  1:4339676/1‑100 (MQ=255)
                                                                               ggTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAccgg                     >  2:1462158/1‑99 (MQ=255)
                                                                                gTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAccggt                    <  2:182099/101‑4 (MQ=255)
                                                                                gTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCAccggt                    <  2:4323901/101‑4 (MQ=255)
                                                                                  cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTc                 <  1:1031136/101‑1 (MQ=255)
                                                                                  cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTc                 >  1:987083/1‑101 (MQ=255)
                                                                                   aGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCg                >  1:3929871/1‑101 (MQ=255)
                                                                                   aGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCg                >  1:443506/1‑101 (MQ=255)
                                                                                    gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg               >  2:3429405/1‑101 (MQ=255)
                                                                                    gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg               <  1:3366920/101‑1 (MQ=255)
                                                                                    gACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCgg               >  1:1591345/1‑101 (MQ=255)
                                                                                       gTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGAAGGGAGCGTGCCAAAGACGGCGGGCGCAAAGGCCGCGCCATAAGTGAGCCCA.CAGGGTCGGAgg            >  1:1533659/1‑101 (MQ=255)
                                                                                        ttGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggt           >  1:2956119/1‑100 (MQ=255)
                                                                                        ttGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGAGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggt           <  1:2307715/101‑2 (MQ=255)
                                                                                         tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt          >  2:3927315/1‑99 (MQ=255)
                                                                                          gtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggttg         <  2:3613391/101‑4 (MQ=255)
                                                                                           tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg        <  2:1072208/101‑5 (MQ=255)
                                                                                           tgCAAAAGC.TCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg        <  2:1730977/101‑5 (MQ=255)
                                                                                               aaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGc   >  2:3000374/1‑101 (MQ=255)
                                                                                               aaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGAGGTTTgatgc    >  1:2288158/1‑97 (MQ=255)
                                                                                                aaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCCGCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCa  >  2:3845596/1‑101 (MQ=255)
                                                                                                     |                                                                                                  
GGCACCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.CGCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCA  >  NC_014034/2040272‑2040470

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG