BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,044,270	Δ1 bp	100%	intergenic (+143/+34)	typA → / ← potG1	GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,044,270	0	A→.	100.0%	47.8	134	intergenic (+143/+34)	typA/potG1	GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand): new base (104/17): ref base (6/7): total (110/24)
Fisher's exact test strand distribution p-value = 2.12e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.09e-01

Only 100 of 166 total aligned reads displayed.


CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGC  >  NC_014034/2044170‑2044367


cGAATGAAATTCCCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCa                                                                                                   <  2:524031/101‑1 (MQ=255)


cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCa                                                                                                   <  1:2561251/101‑1 (MQ=255)


cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCa                                                                                                   >  2:1631023/1‑101 (MQ=255)


cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCa                                                                                                   <  1:1712808/101‑1 (MQ=255)


cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCa                                                                                                   <  1:2079939/101‑1 (MQ=255)


   aTGAAATTCCCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaa                                                                                                <  1:1297032/101‑1 (MQ=255)


    tGAAATTCTCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaa                                                                                               <  1:2700869/101‑1 (MQ=255)


     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaag                                                                                              >  1:3057162/1‑100 (MQ=255)


      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaagg                                                                                             <  2:884756/101‑3 (MQ=255)


        aTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGggg                                                                                           <  2:1509938/101‑1 (MQ=255)


          tCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGggggg                                                                                         >  2:3463430/1‑101 (MQ=255)


           cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggggc                                                                                        >  1:3658761/1‑100 (MQ=255)


           cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggggc                                                                                        >  1:3822119/1‑100 (MQ=255)


            aCCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcg                                                                                      <  2:1421973/101‑1 (MQ=255)


             ccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcgc                                                                                     <  1:1758040/101‑1 (MQ=255)


             ccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcgc                                                                                     <  1:4175617/101‑1 (MQ=255)


              cTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGcgcg                                                                                    <  1:3480006/101‑1 (MQ=255)


               tCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                   >  1:3236841/1‑101 (MQ=255)


                ccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGaa                                                                                  <  1:1906025/101‑1 (MQ=255)


                 cccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAg                                                                                 <  1:4400585/101‑1 (MQ=255)


                  ccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTTCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGc                                                                                >  1:747196/1‑101 (MQ=255)


                  ccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGc                                                                                <  2:2548270/101‑1 (MQ=255)


                   cccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGcc                                                                               <  2:2721271/101‑1 (MQ=255)


                    ccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccc                                                                              >  1:4062119/1‑101 (MQ=255)


                     cGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGcccc                                                                             >  1:4186860/1‑101 (MQ=255)


                     cGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGcccc                                                                             >  1:1434427/1‑101 (MQ=255)


                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccccc                                                                            <  1:4313171/101‑1 (MQ=255)


                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccccc                                                                            >  1:3943374/1‑101 (MQ=255)


                       ggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGcccccc                                                                           >  1:2322725/1‑101 (MQ=255)


                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtt                                                                         >  2:66834/1‑101 (MQ=255)


                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtt                                                                         <  2:750613/101‑1 (MQ=255)


                           atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttt                                                                       >  2:690090/1‑101 (MQ=255)


                             ttGATGCAAGATAAAGACTTGCGAGGGGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCgttgtc                                                                     >  1:3963396/1‑98 (MQ=255)


                             ttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttc                                                                     >  2:1414074/1‑100 (MQ=255)


                              tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttcc                                                                    >  2:238870/1‑99 (MQ=255)


                               gAGGCAAGATAAAGACTTGCGAGGCGGTCTTCATCCTTCTGCAAAGACCCCGGGGGGAGTGTCCAC.GCCAAAAAGGGGGGCGCCAAGCCGCCAtttttccg                                                                   >  2:326973/1‑98 (MQ=255)


                                  gCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCa                                                               >  1:1792545/1‑101 (MQ=255)


                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAg                                                              >  2:508450/1‑101 (MQ=255)


                                    aaaaTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCAGc                                                             <  2:2209983/98‑1 (MQ=255)


                                    aaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGc                                                             >  2:1950683/1‑101 (MQ=255)


                                    aaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGc                                                             <  1:2912583/101‑1 (MQ=255)


                                    aaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGc                                                             >  2:4344305/1‑101 (MQ=255)


                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaa                                                          >  2:5493/1‑101 (MQ=255)


                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaa                                                          >  2:3178485/1‑101 (MQ=255)


                                        tAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaaa                                                         >  1:2129231/1‑101 (MQ=255)


                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGTGCGCGAAGCCCCCC.TTTTTCCGTCACCGAAAc                                                        >  2:2296032/1‑101 (MQ=255)


                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                        >  2:3088931/1‑101 (MQ=255)


                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGACAGCGAAACAg                                                      >  1:1158473/1‑101 (MQ=255)


                                               ttGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCAcc                                                  >  1:1075861/1‑101 (MQ=255)


                                               ttGCGAGGTGTTCTTCATCTTGCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCAcc                                                  >  1:2639243/1‑101 (MQ=255)


                                                tGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCa                                                 >  2:2279846/1‑101 (MQ=255)


                                                  cGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgc                                               >  1:1572998/1‑101 (MQ=255)


                                                    aGGTGTTCTTCAGCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgc                                             >  1:2527570/1‑101 (MQ=255)


                                                        gtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCg                                         >  2:2265695/1‑101 (MQ=255)


                                                        gtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCg                                         >  1:520234/1‑101 (MQ=255)


                                                        gtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAAC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCg                                         >  2:2325200/1‑101 (MQ=255)


                                                         ttcttcATCTTTCTGAAAAAACCGAGGGGGGAATTTGCACAG.GAAAAGGGGGGGCGGGAGGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCgg                                        <  2:346072/101‑1 (MQ=255)


                                                          tcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCggg                                       >  2:307053/1‑101 (MQ=255)


                                                           cttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGc                                      >  1:4243192/1‑101 (MQ=255)


                                                           cttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGc                                      >  2:808739/1‑101 (MQ=255)


                                                            ttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCg                                     >  2:1872960/1‑101 (MQ=255)


                                                            ttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCg                                     >  1:1615162/1‑101 (MQ=255)


                                                            ttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCg                                     >  2:4290278/1‑101 (MQ=255)


                                                              cATCTTTCTGCAAAGCCCGCGGGGGGAATTTGCACAGC.AAAAGGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                   <  1:682479/101‑1 (MQ=255)


                                                              cATCTTTCTGCAAAGACCGCGGGGGGGATTTGCACAGC.AAAAACCGCAGAGCGAAG.CCCCCATTTTTCCGTCAGCGAAACATCACCAACGCGCCCGGCGAc                                   >  2:3882773/1‑101 (MQ=255)


                                                              cATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                   >  1:4099007/1‑101 (MQ=255)


                                                              cATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                   >  1:4071542/1‑101 (MQ=255)


                                                                tCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCa                                 >  1:4610935/1‑101 (MQ=255)


                                                                tCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCa                                 >  1:937624/1‑101 (MQ=255)


                                                                 cTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAg                                >  2:1803837/1‑101 (MQ=255)


                                                                  tttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAgg                               >  1:3095514/1‑101 (MQ=255)


                                                                     cTGCAAAGGCCGCGGGGGGAATTTGCACAG.AAAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                            <  2:3056918/101‑1 (MQ=255)


                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGACACAGCACCAGCGCGCCGGGCGACCAGGCCa                            >  2:758990/1‑101 (MQ=255)


                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                            >  1:2793883/1‑101 (MQ=255)


                                                                      tGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAc                           >  2:3824425/1‑101 (MQ=255)


                                                                      ggcaaggagggcgGGGGGAATCAGCACAG.AAAAGAGGGGGG.GCCCAACCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAc                           <  1:3391589/91‑1 (MQ=255)


                                                                          aaGCCCGGGGGGGGAATTT.CACAAAAAAAAGGGGGGGCCGCAACCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                        <  2:2388019/101‑1 (MQ=255)


                                                                          aaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgc                       >  1:558737/1‑101 (MQ=255)


                                                                          aaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGCCCACCcgc                       >  1:1980972/1‑101 (MQ=255)


                                                                           aGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgcg                      >  2:2765254/1‑101 (MQ=255)


                                                                            gACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgcgc                     >  2:2131662/1‑100 (MQ=255)


                                                                            gACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGt                     >  1:2562959/1‑101 (MQ=255)


                                                                              ccGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCt                   >  1:3062933/1‑101 (MQ=255)


                                                                               cgcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTg                  >  2:4443491/1‑101 (MQ=255)


                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  1:4024272/1‑101 (MQ=255)


                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  1:400064/1‑101 (MQ=255)


                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  1:3326100/1‑101 (MQ=255)


                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  2:3104028/1‑101 (MQ=255)


                                                                                   gggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTg              >  1:328038/1‑101 (MQ=255)


                                                                                    ggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGc             >  1:2231221/1‑101 (MQ=255)


                                                                                    ggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGc             >  2:1928299/1‑101 (MQ=255)


                                                                                     gggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGcc            >  1:3577536/1‑101 (MQ=255)


                                                                                      ggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCg           >  1:4200221/1‑101 (MQ=255)


                                                                                        aaTTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGAc         >  2:2920912/1‑101 (MQ=255)


                                                                                        aaTTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGCGCCGAc         >  1:3862201/1‑101 (MQ=255)


                                                                                         aTTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGACACCCGCGTCTGGGTGCCGCCa        >  2:3964929/1‑101 (MQ=255)


                                                                                          gcccgaacaaaaaaaaGGGGGCCGCCACCCCCCCCATTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGAc         <  1:1270273/91‑1 (MQ=255)


                                                                                            tGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATcc     >  2:4176595/1‑101 (MQ=255)


                                                                                            tGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATcc     >  1:4526124/1‑101 (MQ=255)


                                                                                              cacaGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAg   >  2:1230754/1‑101 (MQ=255)


CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGC  >  NC_014034/2044170‑2044367

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 28 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG