Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,902	Δ1 bp	100%	intergenic (+175/‑330)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,902	0	T→.	100.0%	280.9	69	intergenic (+175/‑330)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (26/42):  ref base (0/0):  total (27/42)

TTCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCC.G.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAA.CCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTC  >  NC_014034/2185802‑2185986                                                                                                       |                                                                                       ttCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGt                                                                                        <  2:472515/101‑1 (MQ=255) ttCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGt                                                                                        <  2:4219766/101‑1 (MQ=255)   cGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttt                                                                                      <  1:4268351/101‑1 (MQ=255)   cGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttt                                                                                      <  1:2149102/101‑1 (MQ=255)      cTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttga                                                                                   <  1:2260589/101‑3 (MQ=37)      cTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttga                                                                                   <  1:2028005/101‑3 (MQ=37)        cggcggTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttgatg                                                                                 <  1:1782658/101‑5 (MQ=37)          gcggTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                                                                              <  1:2152105/101‑1 (MQ=37)           cggTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGca                                                                             <  2:1512858/101‑1 (MQ=37)            ggTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGccc                                                                            >  1:1352495/1‑99 (MQ=25)            ggTGCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcac                                                                            <  1:827531/101‑1 (MQ=37)               gCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAg                                                                         <  2:1030814/101‑1 (MQ=37)               gCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAg                                                                         <  1:2725082/101‑1 (MQ=37)               gCCGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAg                                                                         <  2:2841915/101‑1 (MQ=37)                ccGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGa                                                                        <  2:3397668/101‑1 (MQ=37)                 cGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                       <  2:4425561/101‑1 (MQ=37)                 cGAGGCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                       <  1:641860/101‑1 (MQ=37)                    ggCGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                                                                    <  2:2131358/101‑1 (MQ=37)                      cGACCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGcc                                                                  <  2:2606674/101‑1 (MQ=37)                        aCCCTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGa                                                                <  1:3408880/101‑1 (MQ=37)                         cccTGCGGGGGCTCCAC.CCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGaa                                                               <  1:1087045/101‑1 (MQ=37)                          ccTGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.c                                                             <  2:1203007/101‑1 (MQ=37)                            tGCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCg                                                           <  2:1605340/101‑1 (MQ=37)                             gCGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.c                                                         >  2:3681964/1‑101 (MQ=25)                              cGGGGGCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.cc                                                        >  1:1677143/1‑101 (MQ=37)                                ggggCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACCAGAAACGCCGAA.CCC.cccc                                                      >  1:1312641/1‑101 (MQ=25)                                ggggCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.cccc                                                      <  1:2813353/101‑1 (MQ=37)                                   gCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.CCCCctt                                                   <  2:502260/101‑1 (MQ=37)                                   gCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.CCCCctt                                                   <  1:2590385/101‑1 (MQ=37)                                   gCTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.CCCCctt                                                   <  1:1843519/101‑1 (MQ=37)                                    cTCC.GCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.CCCCcttt                                                  <  1:1488086/101‑2 (MQ=37)                                      tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.CCCCctttc                                                 >  1:2330710/6‑99 (MQ=37)                                       ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCa                                                 <  2:2213473/97‑1 (MQ=37)                                       ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCG.CCCCctttcc                                                >  1:1797228/5‑98 (MQ=38)                                         gcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCAcc                                               <  1:4220803/99‑1 (MQ=38)                                          cccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCt                                              <  2:3119675/100‑1 (MQ=38)                                          cccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCt                                              <  2:4541455/100‑1 (MQ=38)                                                gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCt                                         >  1:4280145/4‑101 (MQ=39)                                                gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCt                                         >  2:3813176/4‑101 (MQ=255)                                                 cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTc                                        <  1:213107/99‑1 (MQ=39)                                                 cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGACCCCTTTCACCTTGGCTc                                        <  1:940393/99‑1 (MQ=39)                                                    ccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCaaa                                     >  2:4566712/1‑101 (MQ=255)                                                     cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAat                                    <  1:3778738/101‑1 (MQ=255)                                                     cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAat                                    <  2:3170923/101‑1 (MQ=255)                                                     cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAat                                    <  1:503206/101‑1 (MQ=255)                                                      ccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAata                                   <  2:2330548/101‑1 (MQ=255)                                                      ccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTT.TTTTTGATGGCACAAGAAACGCCGAACCCC.CCCCTTTCACCTTGGCTCAAata                                   >  1:2166800/1‑101 (MQ=255)                                                        ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAACTATc                                 >  1:2229733/1‑101 (MQ=255)                                                        ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATc                                 <  2:2453051/101‑1 (MQ=255)                                                        ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATc                                 <  1:1089342/101‑1 (MQ=255)                                                          cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAATAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATccc                               <  1:2859911/101‑1 (MQ=255)                                                          cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATccc                               >  1:838381/1‑101 (MQ=255)                                                          cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATccc                               <  2:2846664/101‑1 (MQ=255)                                                           gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCg                              >  2:2907970/1‑101 (MQ=255)                                                           gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCg                              >  1:702103/1‑101 (MQ=255)                                                           gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATACCg                              >  1:3275870/1‑101 (MQ=255)                                                            tATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCgg                             >  2:2570277/1‑101 (MQ=255)                                                              tttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCgggg                           >  2:1593539/1‑101 (MQ=255)                                                              tttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCgggg                           <  1:3197724/101‑1 (MQ=255)                                                                 aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTc                        <  1:3813461/101‑1 (MQ=255)                                                                  tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                       >  2:2368700/1‑101 (MQ=255)                                                                  tCCAAAGAAAAAGCAGCGGCTTGCCCCGAAGGACTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                       <  1:2685057/101‑1 (MQ=38)                                                                   cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                      <  2:1296528/101‑1 (MQ=255)                                                                       aGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCggggg                  >  1:2772505/1‑101 (MQ=255)                                                                           aaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGa              >  2:3707582/1‑101 (MQ=255)                                                                             agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGc            <  1:2119360/101‑1 (MQ=255)                                                                              gcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcc           <  1:1732596/101‑1 (MQ=255)                                                                               cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccc          <  2:2675189/101‑1 (MQ=255)                                                                               cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccc          >  1:828243/1‑101 (MQ=255)                                                                               cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccc          >  2:2041401/1‑101 (MQ=255)                                                                                 gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccccc        >  1:1375967/1‑99 (MQ=37)                                                                                 gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccccc        <  1:3682239/101‑3 (MQ=37)                                                                                  cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccg       <  1:314406/101‑4 (MQ=37)                                                                                  cGGCTTCCCCCGAAGGCC.TGTTTTTGATGCCAAAAGAAACGCGGAA.CCGCCCCCTTTCACCTTGGCCCAAATTCCCCGGGGGTCCGGGGGCGGAGcccccg       <  2:1797309/101‑4 (MQ=18)                                                                                   ggCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccgg      >  1:4653522/1‑97 (MQ=25)                                                                                     cTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcg    >  2:1270081/1‑95 (MQ=25)                                                                                      ttGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcgg   >  1:2738581/1‑94 (MQ=25)                                                                                       tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcggg  >  1:3001391/1‑93 (MQ=21)                                                                                       tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcggg  >  2:1105762/1‑93 (MQ=21)                                                                                       tGCCCCGAAGGCCTT.ATTTTGATGGCACAAGAAACGCCGAA.CCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcggg  >  1:3373897/1‑93 (MQ=21)                                                                                                       |                                                                                       TTCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCC.G.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAA.CCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTC  >  NC_014034/2185802‑2185986 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG