Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,198,736 Δ1 bp 100% coding (133/729 nt) cobA2 ← uroporphyrinogen‑III C‑methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,198,7360C→.100.0% 886.9 200coding (133/729 nt)cobA2uroporphyrinogen‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (97/102):  ref base (0/1):  total (97/103)

Only 100 of 235 total aligned reads displayed.

GCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCC  >  NC_014034/2198636‑2198833
                                                                                                    |                                                                                                 
gcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGC.GGCATGGcc                                                                                                  >  2:3582282/1‑101 (MQ=255)
gcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcc                                                                                                  <  2:2593732/101‑1 (MQ=255)
 cATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccc                                                                                                 >  1:3433131/1‑101 (MQ=255)
 cATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccc                                                                                                 <  2:2789596/101‑1 (MQ=255)
 cATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccc                                                                                                 <  2:4209292/101‑1 (MQ=255)
 cATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccc                                                                                                 >  1:4668238/1‑101 (MQ=255)
  aTGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccca                                                                                                <  2:1545913/101‑2 (MQ=255)
   tGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccag                                                                                               <  2:755037/101‑3 (MQ=255)
      tCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGAt                                                                                           <  1:1089396/101‑1 (MQ=255)
      tCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGAt                                                                                           <  2:585060/101‑1 (MQ=255)
       cgacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATc                                                                                          >  1:4014441/1‑101 (MQ=255)
        gacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CACCCGGGCGGGCATGG.CCCAGGATCg                                                                                         >  2:1562917/1‑101 (MQ=255)
         acgaGCAAGCGGCTCACATGATCCTGCTTCGGCGATGGCCGCCCTGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGGATGG.CCCAGGATCgg                                                                                        >  2:3092464/1‑101 (MQ=255)
         acgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCgg                                                                                        >  1:4099991/1‑101 (MQ=255)
           gaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcc                                                                                      >  2:1249873/1‑101 (MQ=255)
             gCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccg                                                                                    <  2:2412468/101‑2 (MQ=255)
                 gCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCcggcgg                                                                               <  2:2049195/101‑1 (MQ=255)
                  cGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGa                                                                              <  2:839615/101‑1 (MQ=255)
                  cGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGa                                                                              <  2:2323147/101‑1 (MQ=255)
                   ggCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaa                                                                             >  1:2397059/1‑101 (MQ=255)
                    gCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaaa                                                                            <  2:51390/101‑1 (MQ=255)
                      tCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGa                                                                          <  2:3316442/101‑1 (MQ=255)
                        acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCACGATCGG.CCCGGCGGAAAGAtc                                                                        <  1:2639406/101‑1 (MQ=255)
                         caTGATCCTTCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcg                                                                       <  2:670004/101‑1 (MQ=255)
                         caTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcg                                                                       >  1:663672/1‑101 (MQ=255)
                          aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                                      >  2:1608016/1‑101 (MQ=255)
                          aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                                      >  2:927563/1‑101 (MQ=255)
                          aTGATCCTGCTTCGGCGAGGCCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGCAAGAtcgc                                                                      >  2:2371706/1‑100 (MQ=255)
                             aTCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGa                                                                   <  1:3291723/101‑1 (MQ=255)
                              tCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGaa                                                                  <  1:4551809/101‑1 (MQ=255)
                                  gCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGc                                                              >  1:3380524/1‑101 (MQ=255)
                                  gCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGc                                                              >  1:1769833/1‑101 (MQ=255)
                                   cTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAccgtgacatcggc                                                             <  2:225861/101‑10 (MQ=255)
                                    ttCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcac                                                            >  2:1934336/1‑101 (MQ=255)
                                    ttCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcac                                                            >  1:4398760/1‑101 (MQ=255)
                                      cGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcacca                                                          >  2:245516/1‑101 (MQ=255)
                                       ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                                         <  2:1451285/101‑1 (MQ=255)
                                       ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                                         >  1:966130/1‑101 (MQ=255)
                                         cGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACAt                                                       <  2:1769714/101‑1 (MQ=255)
                                          gAGGGCCGCCCGGCCCGCTTTCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.GCCGGCGGAAAGATCGTCGAACAGCACCACATc                                                      >  2:287390/1‑101 (MQ=255)
                                          gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                                      <  1:3912517/101‑1 (MQ=255)
                                           aGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCg                                                     <  1:4118082/101‑1 (MQ=255)
                                           aGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCg                                                     >  2:3493041/1‑101 (MQ=255)
                                            gggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCgg                                                    <  1:1554642/101‑1 (MQ=255)
                                            gggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCgg                                                    <  1:4104654/101‑1 (MQ=255)
                                              gccgccCGGCCCGCTTGCCGACCGCGCTGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGcc                                                  <  1:2803903/101‑1 (MQ=255)
                                              gccgccCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGcc                                                  >  2:184964/1‑101 (MQ=255)
                                                cgccCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCtt                                                <  2:2686785/101‑1 (MQ=255)
                                                 gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTg                                               <  2:3534223/101‑1 (MQ=255)
                                                  cccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTgg                                              <  2:3783627/101‑1 (MQ=255)
                                                   ccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGc                                             <  2:1651871/101‑1 (MQ=255)
                                                    cggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCg                                            <  2:3363729/101‑1 (MQ=255)
                                                     ggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGa                                           >  1:1360987/1‑101 (MQ=255)
                                                       cccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGc                                         <  1:1249966/101‑1 (MQ=255)
                                                        ccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGcc                                        <  1:2839623/101‑1 (MQ=255)
                                                          gCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCgg                                      >  2:2483906/1‑101 (MQ=255)
                                                          gCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCgg                                      >  1:887856/1‑101 (MQ=255)
                                                           cTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGt                                     >  1:4470740/1‑101 (MQ=255)
                                                            ttGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTc                                    <  2:922100/101‑1 (MQ=255)
                                                            ttGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTc                                    >  2:2110495/1‑101 (MQ=255)
                                                                cGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACACCCCCACATCGGCCTTGGCGAGCCGGTCGGCa                                <  1:4157881/101‑1 (MQ=255)
                                                                 gACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGGGAGCCGACCGGCAg                               >  2:1915887/1‑101 (MQ=255)
                                                                    cgcgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCtt                            <  1:2986768/101‑1 (MQ=255)
                                                                      cgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGGAGCTTTc                          >  1:824172/1‑101 (MQ=255)
                                                                       gATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCa                         >  2:59085/1‑101 (MQ=255)
                                                                        aTGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCac                        >  1:1424949/1‑100 (MQ=255)
                                                                        aTGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAg                        >  1:3648458/1‑101 (MQ=255)
                                                                        aTGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAg                        >  1:4612917/1‑101 (MQ=255)
                                                                        aTGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAg                        >  2:3400867/1‑101 (MQ=255)
                                                                           aGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGt                     <  1:1966923/101‑1 (MQ=255)
                                                                           aGTTCGG.CCCCCAGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGt                     >  1:2529214/1‑101 (MQ=255)
                                                                             ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa                   <  1:1473193/101‑1 (MQ=255)
                                                                               tcggcccccGGGCGGGCCTGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAg                  <  2:4184707/97‑1 (MQ=255)
                                                                                cggcccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCCGCTTTCAGCGTCAGa                 >  1:1802462/4‑101 (MQ=255)
                                                                                cggcccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGa                 <  2:1981576/98‑1 (MQ=255)
                                                                                 ggcccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGaa                <  1:2110645/99‑1 (MQ=255)
                                                                                 gCCCCCCGGGCGGGCCTCGCCCC.GGAAC.GCCCCCGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGaa                <  2:824096/101‑1 (MQ=255)
                                                                                  gcccccGGGCGGGCCTGG.CCGAGTATCGG.CCCGGCGGAAAGATCGTCGAACAGCGCCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAg               <  2:1188188/100‑1 (MQ=255)
                                                                                  gcccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAg               <  1:3835714/100‑1 (MQ=255)
                                                                                  gcccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAg               >  2:2956427/2‑101 (MQ=255)
                                                                                   cccccGGGCGGGCATGG.CCCGGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGc              <  2:4594442/101‑1 (MQ=255)
                                                                                   cccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGc              <  1:2694655/101‑1 (MQ=255)
                                                                                    ccccGGGCGGGCATGG.CCGAGGAGC.GCCCCGGGGGAAGGATGGTCGAACAGCACCACACCGGCCTGGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCt             <  2:440378/101‑1 (MQ=255)
                                                                                     cccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTc            <  1:539568/101‑1 (MQ=255)
                                                                                      ccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTcc           <  2:3698152/101‑1 (MQ=255)
                                                                                      ccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTcc           >  1:2200089/1‑101 (MQ=255)
                                                                                      ccGGGCCGGCA.GGGCCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCACATCGGCCTGGGGGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTcc           <  1:3582550/101‑1 (MQ=255)
                                                                                       cgggcgggCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCg          <  1:1570789/101‑1 (MQ=255)
                                                                                       cgggcgggCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCg          >  1:24393/1‑101 (MQ=255)
                                                                                       cgggcgggCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCg          >  2:2754303/1‑101 (MQ=255)
                                                                                        gggcgggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCgg         <  1:1151147/101‑1 (MQ=255)
                                                                                         ggcgggcATGG.CCCAGGATCGG.CCGGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGa        <  2:4398468/101‑1 (MQ=255)
                                                                                          gcgggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCACACCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGAt       <  2:4564071/101‑1 (MQ=255)
                                                                                           cgggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATc      >  2:4301485/1‑101 (MQ=255)
                                                                                            gggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCg     <  1:1023834/101‑1 (MQ=255)
                                                                                            gggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCg     <  2:4004984/101‑1 (MQ=255)
                                                                                             ggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGc    >  1:3727775/1‑101 (MQ=255)
                                                                                             ggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGc    >  1:1716164/1‑101 (MQ=255)
                                                                                               cATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGccc  >  2:1114422/1‑101 (MQ=255)
                                                                                               cATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGccc  >  1:793388/1‑101 (MQ=255)
                                                                                                    |                                                                                                 
GCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCC  >  NC_014034/2198636‑2198833

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG