Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,236,666 Δ1 bp 2.9% intergenic (+17/‑112) RCAP_rcc02069 → / → RCAP_rcc02070 hypothetical protein/ArsR family transcriptional regulator/protein tyrosine phosphatase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,236,6660C→. 2.9% 7.5 ‑1.0 ‑0.3172intergenic (+17/‑112)RCAP_rcc02069/RCAP_rcc02070hypothetical protein/ArsR family transcriptional regulator/protein tyrosine phosphatase
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (84/83):  total (88/84)
Fisher's exact test strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.10e-01

Only 100 of 211 total aligned reads displayed.

CCTGGGAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGT  >  NC_014034/2236567‑2236766
                                                                                                   |                                                                                                    
ccTGGGAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGcc                                                                                                     >  2:2870846/1‑101 (MQ=255)
   gggAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGccccc                                                                                                  >  2:3841105/1‑101 (MQ=255)
     gAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGGCGCCTGccccccc                                                                                                >  2:757192/1‑101 (MQ=255)
     gAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGccccccc                                                                                                >  1:1580043/1‑101 (MQ=255)
        cgccggtggggCGATCCCGAGTTTTTCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCt                                                                                             <  2:2976512/93‑1 (MQ=255)
              gtGGGCGATGCCGGGGTTTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGccccc                                                                                       <  1:4284021/100‑1 (MQ=255)
              gggggCGATGCCGAGTATTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGccccc                                                                                       <  2:3338820/99‑1 (MQ=255)
               ggggcgggcccGGGGTTTTCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCg                                                                                      <  2:1358285/92‑1 (MQ=255)
                    gATGCCGAGTTTTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACg                                                                                 <  1:2744939/101‑1 (MQ=255)
                          gAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCCGCCCCCGCACCCGgcgcc                                                                           >  1:3466910/1‑100 (MQ=255)
                           agttttccccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAg                                                                          <  2:2440878/92‑1 (MQ=255)
                           aGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGGCGGGGAGCGCTCTGCCGCGCTCGACCGCCTGACCACCCCCTGCCCCCGCCACGGGCGCAg                                                                          >  2:2066860/1‑101 (MQ=255)
                           aGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTG.CCCCCCCCTGCCCCCGCAACGGGCGCAGt                                                                         >  1:3450/1‑101 (MQ=255)
                              aTTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCt                                                                       >  1:363634/1‑101 (MQ=255)
                                ttCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGc                                                                     >  2:1065608/1‑101 (MQ=255)
                                tccccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGc                                                                     <  1:1113553/97‑1 (MQ=255)
                                 ccccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGca                                                                    <  1:2975386/98‑1 (MQ=255)
                                  cccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGcac                                                                   <  1:922318/99‑1 (MQ=255)
                                  cccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGcac                                                                   <  1:3870534/99‑1 (MQ=255)
                                  cccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGcac                                                                   <  1:2569462/99‑1 (MQ=255)
                                  cACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGcac                                                                   >  1:3715383/1‑101 (MQ=255)
                                   ccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGcaca                                                                  <  2:1481801/100‑1 (MQ=255)
                                   ccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGcaca                                                                  <  1:4614530/100‑1 (MQ=255)
                                     cgggcgtttCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCACTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGt                                                                <  1:1063754/94‑1 (MQ=255)
                                        ccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcg                                                             <  1:3103799/101‑1 (MQ=255)
                                        ccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcg                                                             <  2:3490660/101‑1 (MQ=255)
                                        ccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcg                                                             <  2:2480923/101‑1 (MQ=255)
                                         cgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcgg                                                            <  1:3532600/101‑1 (MQ=255)
                                         cgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcgg                                                            >  2:2593484/1‑101 (MQ=255)
                                          gcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggc                                                           <  2:1200625/101‑1 (MQ=255)
                                          gcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggc                                                           >  2:1344223/1‑101 (MQ=255)
                                          gcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggc                                                           >  1:1447936/1‑101 (MQ=255)
                                          gcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggc                                                           >  1:1260066/1‑101 (MQ=255)
                                          gcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggc                                                           >  1:4577074/1‑101 (MQ=255)
                                            ttCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggcgg                                                         >  1:708192/1‑101 (MQ=255)
                                               gAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGgcgc                                                      >  2:4085683/1‑101 (MQ=255)
                                               gAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGgcgc                                                      >  2:3707901/1‑101 (MQ=255)
                                               gAAGCTGCGCTGCGGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGgcgc                                                      <  1:3996130/101‑1 (MQ=255)
                                                aaGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCCGCCCCCCCCCCAGGGGCGCACTCCCCAGTCCCGCGGCGcc                                                     >  2:4550452/1‑101 (MQ=255)
                                                aaGCTGCGCTGCGGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGcc                                                     <  1:1993703/101‑1 (MQ=255)
                                                 aGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGGACAGTCCGGCGGGGccc                                                    >  2:2015064/1‑100 (MQ=255)
                                                   ctgcgctgcTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGt                                                  <  1:44606/101‑1 (MQ=255)
                                                    tgcgctgcGGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTc                                                 <  2:610341/101‑1 (MQ=255)
                                                    cgcgctgcGGGCGGGCCGGGCCGGGCTCTGACGCGCTCGTCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTc                                                 <  2:473228/100‑1 (MQ=255)
                                                     gcgctgcTGGCGGCCCGGGCCGCGCTCTGACGCGCTCGGTCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCg                                                <  1:2126735/101‑1 (MQ=255)
                                                       gctgctGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGcc                                              <  1:4430115/101‑1 (MQ=255)
                                                       gctgctGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGcc                                              <  2:1265884/101‑1 (MQ=255)
                                                        ctgctgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCCCCACCGGCGCAGGCTGCCCCGTCCGGCGGCGCCCGGCGccc                                             >  1:846953/1‑100 (MQ=255)
                                                         tgctgGCGGGCCGGGCCGCGCTCTGCCGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCgg                                            <  1:3942214/101‑1 (MQ=255)
                                                         tgctgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGGCCGTCGCCgg                                            >  2:2791194/1‑101 (MQ=255)
                                                           ctgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGAt                                          <  1:61145/101‑1 (MQ=255)
                                                           cgggCGGCCCGGCCCCCGCTCTGCCGCGCCCGGCCCCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGAt                                          <  1:2793500/99‑1 (MQ=255)
                                                            tgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGAtt                                         >  1:1355476/1‑101 (MQ=255)
                                                            gggCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGAtt                                         <  1:510283/100‑1 (MQ=255)
                                                             ggCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTg                                        <  1:2871062/101‑1 (MQ=255)
                                                                gggccgggccGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCa                                     >  1:2477180/1‑101 (MQ=255)
                                                                 ggccgggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCAt                                    >  2:4058239/1‑101 (MQ=255)
                                                                 ggccgggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCAt                                    <  1:1178585/101‑1 (MQ=255)
                                                                 ggccgggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCAt                                    >  1:759793/1‑101 (MQ=255)
                                                                  gccgggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATg                                   <  2:1710957/101‑1 (MQ=255)
                                                                  gccgggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATg                                   <  1:2291679/101‑1 (MQ=255)
                                                                   ccgggccgCGCTCTGACGCGCTCGCCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGa                                  <  2:4594517/101‑1 (MQ=255)
                                                                    cgggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGaa                                 >  2:3398419/1‑101 (MQ=255)
                                                                    cgggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGaa                                 <  2:2834451/101‑1 (MQ=255)
                                                                     gggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCCACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGaaa                                >  1:1517504/1‑101 (MQ=255)
                                                                      ggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAg                               <  2:1619334/101‑1 (MQ=255)
                                                                      ggccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAg                               <  1:175607/101‑1 (MQ=255)
                                                                       gccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCCGCCCCCGCAACGGGCGCAGTCTGCACAGGCCGGCGGCGCCTGTCGCCGGACTGTCATAAAAGc                              >  2:4207675/1‑101 (MQ=255)
                                                                        ccgCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCATTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCt                             >  1:3921278/1‑101 (MQ=255)
                                                                         cgcgCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCtt                            <  1:3841390/101‑1 (MQ=255)
                                                                          gcgcTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTc                           <  1:565018/101‑1 (MQ=255)
                                                                           gcctctGACGCGATCGGCAGCCTGCCCCACCCCTGCCCCCGCAACGGGCGCAGTCTGCACACTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCa                          <  1:1834713/99‑1 (MQ=38)
                                                                            gcTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCAc                         >  1:688589/1‑101 (MQ=255)
                                                                               ctGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTgg                      >  2:3242876/1‑101 (MQ=255)
                                                                                tgattcgcTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGGTTGTCATGAAAGCTTCCCTGGa                     >  2:3949240/6‑101 (MQ=255)
                                                                                tGACGCGCTCGGCCGCCTGCCCCCCCCCT.CCCCCCCAAACGGCGCAGTCCTGACCGGCCGGGGGGGCCTGTCGCCCGGTTGTCATGGAAACtttccttggc                    >  2:462879/1‑93 (MQ=255)
                                                                                ggACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCTGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGa                     <  2:3619691/100‑1 (MQ=255)
                                                                                  gcgcgCTCGGCCCCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACg                   <  1:4646183/100‑1 (MQ=255)
                                                                                   cgcgCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACgg                  <  1:1680757/101‑1 (MQ=255)
                                                                                      gcccGGCCCCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACggggg               <  1:1326021/98‑1 (MQ=255)
                                                                                      gcTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGGGCCTGTCGCCGGATTGTCATTAAAGCTTCACCGGACggggg               >  2:1902136/1‑101 (MQ=255)
                                                                                      gcTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAACTTCACTGGACggggg               >  2:3789033/1‑101 (MQ=255)
                                                                                       cTCGGCCGCCTG.CCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGa             >  2:643164/1‑101 (MQ=255)
                                                                                         cGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAt            >  1:3149093/1‑101 (MQ=255)
                                                                                          ggCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAtt           >  2:1789225/1‑101 (MQ=255)
                                                                                          ggCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAtt           >  2:515535/1‑101 (MQ=255)
                                                                                           gccgccTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTg          >  1:954441/1‑101 (MQ=255)
                                                                                           atctccTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTg          <  2:2651971/97‑1 (MQ=255)
                                                                                            ccgccTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGc         >  1:2625318/1‑101 (MQ=255)
                                                                                            ccgccCGCCCCCCCACAGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGc         <  1:1346515/101‑1 (MQ=255)
                                                                                             cgccTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCt        <  1:4085969/101‑1 (MQ=255)
                                                                                              gccTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGGTTGCtt       >  1:1468005/1‑101 (MQ=255)
                                                                                              cccctcccccccccTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCtt       <  2:2952858/96‑1 (MQ=255)
                                                                                               ccTGCCCCCCCCCTGCCCCCGGAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTgagat      >  1:2652156/1‑97 (MQ=255)
                                                                                               ccTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTc      <  1:864158/101‑1 (MQ=255)
                                                                                                 tGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCt    >  1:1892225/1‑101 (MQ=255)
                                                                                                 tGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCt    <  2:707387/101‑1 (MQ=255)
                                                                                                 tGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCt    <  2:3096128/101‑1 (MQ=255)
                                                                                                  gCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTg   <  2:1559890/101‑1 (MQ=255)
                                                                                                   cccccccccTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGCATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGt  <  2:2380626/101‑1 (MQ=255)
                                                                                                   |                                                                                                    
CCTGGGAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCCTGCCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGT  >  NC_014034/2236567‑2236766

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG