Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,358 Δ1 bp 3.4% intergenic (‑154/‑151) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3580G→. 3.4% 13.3 ‑0.8 ‑0.1203intergenic (‑154/‑151)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (103/93):  total (108/95)
Fisher's exact test strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.44e-01

Only 100 of 225 total aligned reads displayed.

CCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAC  >  NC_014034/2249259‑2249456
                                                                                                            |                                                                                                        
  ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                                                                        >  2:6728/1‑101 (MQ=255)
  ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggg                                                                                                        >  2:2434816/1‑101 (MQ=255)
   gggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCgggg                                                                                                       <  1:3865195/101‑1 (MQ=255)
    ggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggggg                                                                                                      <  2:780584/101‑1 (MQ=255)
    ggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCggggg                                                                                                      >  2:4265205/1‑101 (MQ=255)
     gCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                                     >  1:1395678/1‑101 (MQ=255)
       aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                                                   >  1:1649433/1‑99 (MQ=255)
        agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGTTATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                                                                                  >  1:973071/1‑101 (MQ=38)
        agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                                  <  1:1215774/101‑1 (MQ=255)
        agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                                                  <  2:3774895/101‑1 (MQ=255)
          aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                                               <  1:4507158/101‑1 (MQ=255)
           agtgggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                              >  1:2665358/4‑101 (MQ=255)
           agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTTAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                              <  2:2233459/101‑1 (MQ=255)
           agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                              <  1:3048209/101‑1 (MQ=255)
           agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                              <  2:690510/101‑1 (MQ=255)
           agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                                                              <  2:839540/101‑1 (MQ=255)
           agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCAAAAATATCCCGGGGGGTGAA.Tg                                                                                              <  1:1580504/101‑1 (MQ=255)
            gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                                                             <  1:2957826/101‑1 (MQ=255)
                gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                         <  2:2587633/101‑1 (MQ=255)
                gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                         >  1:1977196/1‑101 (MQ=255)
                gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGAGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                                                         <  1:4045652/101‑1 (MQ=255)
                  tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..Gc                                                                                     >  1:2466060/6‑101 (MQ=255)
                   ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                    <  2:3403963/97‑1 (MQ=255)
                    tgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCa                                                                                   <  1:48882/98‑1 (MQ=255)
                     gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                  >  2:3371068/3‑101 (MQ=255)
                     gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                                                  <  2:1109557/99‑1 (MQ=255)
                      cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                                 >  1:649210/2‑101 (MQ=255)
                       ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                                                >  1:3318288/1‑101 (MQ=255)
                        cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                                                               <  2:2348383/101‑1 (MQ=255)
                         ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                              >  1:1183387/1‑101 (MQ=255)
                         ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                              >  2:3700126/1‑101 (MQ=255)
                         ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAggg                                                                              >  1:4304189/1‑101 (MQ=255)
                          cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                             >  1:679210/1‑101 (MQ=255)
                            tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCTTCTTGCCGAAAAAATCCCGGGGGGGGAA.GGGGCCA..GCCAGAgggggg                                                                           >  2:4001927/1‑101 (MQ=37)
                            tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                           >  1:3661675/1‑101 (MQ=255)
                            tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                                                           >  1:2773954/1‑101 (MQ=255)
                             cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                          <  1:3756107/101‑1 (MQ=255)
                             cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                          <  2:3544450/101‑1 (MQ=255)
                             cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                          >  2:4579212/1‑101 (MQ=255)
                              ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                         <  2:156629/101‑1 (MQ=255)
                               cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                        <  1:2241603/101‑1 (MQ=255)
                               cgtCCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...TGGG..C..GCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCCCA..GCCAGAGGGGGGCAg                                                                        <  2:3852007/101‑1 (MQ=25)
                               cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                                                            <  2:2140257/94‑1 (MQ=18)
                                gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                           >  2:143041/7‑101 (MQ=18)
                                gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                           >  2:2807528/7‑101 (MQ=18)
                                 tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g                                                                    <  2:4216260/101‑1 (MQ=255)
                                  gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g.c                                                                  >  1:3413164/1‑101 (MQ=255)
                                   ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cc                                                                 >  2:3461441/1‑101 (MQ=255)
                                    cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc                                                                >  2:3060633/1‑101 (MQ=255)
                                    cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc                                                                <  2:4022810/101‑1 (MQ=255)
                                     gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                               >  2:2321797/1‑101 (MQ=255)
                                     gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                               >  1:4100865/1‑101 (MQ=255)
                                     cggccT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cccc                                                               <  2:3154963/97‑1 (MQ=255)
                                     cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c.g                                                                    <  1:308289/100‑1 (MQ=18)
                                          t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttc                                                          >  2:538975/1‑97 (MQ=255)
                                            cccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccccccttcc                                                         >  2:1358030/1‑96 (MQ=255)
                                             ccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTcgc                                                         <  2:1977061/101‑1 (MQ=255)
                                              cccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTcgcg                                                        <  1:1250189/101‑1 (MQ=255)
                                                cGGGATATTTGCGGCAAGATGAA...GGGG..CATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCtgccc                                                         >  1:3361751/1‑97 (MQ=0)
                                                cGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcggg                                                      >  1:1804492/1‑101 (MQ=255)
                                                 gggATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCCCCtgcccc                                                        >  2:3008472/1‑96 (MQ=2)
                                                 gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCgcgggc                                                     >  1:3520456/1‑101 (MQ=255)
                                                    atatTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGggcggc                                                  <  2:1624353/101‑1 (MQ=255)
                                                       tttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCcgc                                               <  1:3508841/101‑1 (MQ=255)
                                                            ggCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGAcgc                                          <  1:1910438/101‑1 (MQ=255)
                                                              cAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.AGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCTTGCGGCCGCGAcgcgc                                        >  1:779436/1‑101 (MQ=25)
                                                                aGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGCCgcgcgc                                      >  1:4456455/1‑101 (MQ=255)
                                                                 gATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                     >  2:2469247/1‑101 (MQ=255)
                                                                 gATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc                                     <  1:2975977/101‑1 (MQ=255)
                                                                   tGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGcgccgc                                   >  2:1996427/1‑101 (MQ=255)
                                                                   tGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCC.GGGGGTGAA.AGGCGCA..GCCAGAAGGGGGGAG.CGG.CCCCCCTCGCCGGCGGCCGCGGCGCGCGCCGCa                                  >  2:4505292/1‑101 (MQ=25)
                                                                      a...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGAAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                <  1:4115703/101‑1 (MQ=255)
                                                                          gggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAG.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                               >  1:4271465/1‑101 (MQ=255)
                                                                          gggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                               <  1:1446647/101‑1 (MQ=255)
                                                                          gggg..g..gCTTTCATCTTGCCGAAAATAGCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                               <  1:4622264/101‑1 (MQ=255)
                                                                           ggg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAg                              >  1:3794724/1‑101 (MQ=255)
                                                                            gg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                             <  2:4111817/101‑1 (MQ=255)
                                                                                     tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                        <  1:321237/101‑1 (MQ=255)
                                                                                     tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                        >  2:112372/1‑101 (MQ=255)
                                                                                     tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                        <  1:4387094/101‑1 (MQ=255)
                                                                                      ttCATCTTGCCGAAAATATCCCGGGTGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGc                       >  1:2608560/1‑101 (MQ=37)
                                                                                      ttCATCATGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                       <  1:768392/101‑1 (MQ=255)
                                                                                        cATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                     <  1:4206859/101‑1 (MQ=255)
                                                                                         aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGCCGTGcccc                    >  1:2445144/1‑101 (MQ=255)
                                                                                           cTTGCGGAAAATATCCGGGGGGGTGAA.GGGCGCA..GCCGGGGGGGGGCAGCC.C.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                  <  1:997103/101‑1 (MQ=255)
                                                                                           cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                  >  2:2626868/1‑101 (MQ=255)
                                                                                           cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                  >  2:2570841/1‑101 (MQ=255)
                                                                                            ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                 >  2:2635338/1‑101 (MQ=255)
                                                                                            ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                 <  1:3628035/101‑1 (MQ=255)
                                                                                                cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa             >  1:1066059/1‑101 (MQ=255)
                                                                                                 gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt            <  1:2321959/101‑1 (MQ=255)
                                                                                                  aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt           >  1:2161595/1‑101 (MQ=255)
                                                                                                  aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt           >  1:3955649/1‑101 (MQ=255)
                                                                                                   aattcgccaggggggGGAG.TGCGGCA..GCGAGCGGGGGGCAC.C.GCCCCCCTCCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc          <  1:203184/92‑1 (MQ=255)
                                                                                                    aagcccgtggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt         <  1:594813/93‑1 (MQ=38)
                                                                                                      tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt       <  1:523221/101‑1 (MQ=255)
                                                                                                      tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGAGCCCCTTTGTCATTCTgc       >  1:3826008/1‑100 (MQ=255)
                                                                                                          ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCa   >  1:927373/1‑101 (MQ=255)
                                                                                                           cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc  <  2:3898244/101‑1 (MQ=255)
                                                                                                           cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCCCCTTCGAGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc  >  2:1807724/1‑101 (MQ=255)
                                                                                                            |                                                                                                        
CCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAC  >  NC_014034/2249259‑2249456

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG