Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,470,253 Δ1 bp 98.4% intergenic (‑186/+31) RCAP_rcc02294 ← / ← proB signal transduction histidine kinase/glutamate 5‑kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,470,2530G→.98.4% 2.3 ‑1.3 ‑0.0182intergenic (‑186/+31)RCAP_rcc02294/proBsignal transduction histidine kinase/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  new base (74/105):  ref base (0/3):  total (74/108)
Fisher's exact test strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.54e-01

Only 100 of 202 total aligned reads displayed.

CCTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTC  >  NC_014034/2470153‑2470349
                                                                                                    |                                                                                                
ccTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAg                                                                                                  <  2:186875/101‑1 (MQ=255)
 cTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgg                                                                                                 >  1:4660819/1‑101 (MQ=255)
  tGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAggg                                                                                                <  1:862173/101‑1 (MQ=255)
  tGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAggg                                                                                                <  1:2738221/101‑1 (MQ=255)
   ggCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggg                                                                                               <  1:1096008/101‑1 (MQ=255)
     cTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggg                                                                                             <  2:4054820/101‑1 (MQ=255)
      ttCGGTGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGGCGCCCGCGCGCATACACGGGTGTTGATGAAACCAAAggggggg                                                                                            >  1:283661/1‑101 (MQ=255)
      ttCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAggggggg                                                                                            >  2:1322294/1‑101 (MQ=255)
        cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          <  1:3695912/101‑3 (MQ=255)
        cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          >  1:230485/1‑99 (MQ=255)
         ggCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcc                                                                                         <  1:1585118/101‑1 (MQ=255)
          gcgcGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcct                                                                                        <  1:2587328/101‑2 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      >  2:93184/1‑101 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  1:3880450/101‑1 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      >  1:3525780/1‑101 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  1:352016/101‑1 (MQ=255)
           cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      >  1:134478/1‑101 (MQ=255)
           cgcgGGACCGTTGATGACGCGCGGGCGCCCAAGGAGCCCGCCCGGATCAACGCCGCCCGCGCGCAACCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  2:1778465/101‑1 (MQ=255)
            gcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGc                                                                                     >  1:4232105/1‑101 (MQ=255)
            gcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACACCACCCGAGCGCAAACCCGAGCGTTGACGAAACCAAAGGGGGGGGcctgc                                                                                      >  2:1839765/1‑98 (MQ=255)
              ggggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccc                                                                                   >  1:3783608/1‑101 (MQ=255)
              ggggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccc                                                                                   <  1:2780364/101‑1 (MQ=255)
               gggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccc                                                                                  <  1:2275608/101‑1 (MQ=255)
                ggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccccc                                                                                 >  1:3088152/1‑101 (MQ=255)
                  aCGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCct                                                                               <  1:3770584/101‑1 (MQ=255)
                    gTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctct                                                                             <  1:2654335/101‑1 (MQ=255)
                     ttGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTg                                                                            <  2:472939/101‑1 (MQ=255)
                       gatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGcc                                                                          >  1:1672588/1‑101 (MQ=255)
                        atgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGccc                                                                         >  2:1487695/1‑101 (MQ=255)
                          gaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCg                                                                       <  1:1966435/101‑1 (MQ=255)
                          gaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCg                                                                       <  1:1905573/101‑1 (MQ=255)
                          gaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCg                                                                       <  2:1600350/101‑1 (MQ=255)
                           aCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCgg                                                                      <  1:2221048/101‑1 (MQ=255)
                            cgcgcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGc                                                                     <  1:1846159/101‑1 (MQ=255)
                             gcgcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGcc                                                                    <  1:4209310/101‑1 (MQ=255)
                                cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 >  1:2934905/1‑101 (MQ=255)
                                cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 <  2:337223/101‑1 (MQ=255)
                                 gggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAg                                                                >  2:151965/1‑101 (MQ=255)
                                 gggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAg                                                                <  2:647977/101‑1 (MQ=255)
                                  ggCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGc                                                               <  2:36349/101‑1 (MQ=255)
                                  cttccgatctgAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGc                                                               <  1:2510150/91‑1 (MQ=255)
                                   gcgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGcc                                                              <  2:539726/101‑1 (MQ=255)
                                   gcgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGcc                                                              >  2:3207783/1‑101 (MQ=255)
                                   gcgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGcc                                                              <  2:720196/101‑1 (MQ=255)
                                      ccAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGAt                                                           >  1:1044394/1‑101 (MQ=255)
                                       cAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATc                                                          >  2:2415691/1‑101 (MQ=255)
                                        aaaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCa                                                         <  1:767599/101‑1 (MQ=255)
                                         aaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAc                                                        >  1:206307/1‑101 (MQ=255)
                                         aaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAc                                                        >  1:3308309/1‑101 (MQ=255)
                                           ggAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAccg                                                      <  2:3525522/101‑2 (MQ=255)
                                            gAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCAGATcggaag                                                     >  2:2509976/1‑96 (MQ=255)
                                              aCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgt                                                   <  2:1910628/101‑1 (MQ=255)
                                              aCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgt                                                   <  2:119658/101‑1 (MQ=255)
                                                  gcCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtcgtc                                               >  2:395435/1‑101 (MQ=255)
                                                  gcCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtcgtc                                               <  1:3558321/101‑1 (MQ=255)
                                                   cccGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGtcgtcg                                              <  2:2713890/101‑1 (MQ=255)
                                                    ccGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTcgc                                             <  2:3488894/101‑1 (MQ=255)
                                                    ccGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTcgc                                             >  1:1752257/1‑101 (MQ=255)
                                                     cGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTcgcg                                            >  2:2344597/1‑101 (MQ=255)
                                                       gATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGAt                                          <  1:1246811/101‑1 (MQ=255)
                                                        aTCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATg                                         >  1:739752/1‑101 (MQ=255)
                                                         tCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGc                                        >  2:2961065/1‑101 (MQ=255)
                                                          cAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGca                                       <  1:2390208/101‑1 (MQ=255)
                                                           aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                      <  2:4231276/101‑1 (MQ=255)
                                                           aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                      <  1:2102554/101‑1 (MQ=255)
                                                           aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                      <  2:3997514/101‑1 (MQ=255)
                                                           aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                      >  2:3821167/1‑101 (MQ=255)
                                                            aCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcaca                                     <  1:624271/101‑1 (MQ=255)
                                                             cgccgcCCGCGCGGAACCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACaa                                    <  2:1641394/101‑1 (MQ=255)
                                                              gccgccCGCGCGCAAACCCGGGGGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAg                                   >  2:4509677/1‑101 (MQ=255)
                                                                 ccccgcGCGCAAACCCGGGTGTTGATTAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgc                                <  2:1825697/100‑1 (MQ=255)
                                                                  cccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcg                               <  2:283629/101‑1 (MQ=255)
                                                                   ccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcgg                              <  2:1292334/101‑1 (MQ=255)
                                                                    cgcgcgCAACCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcggc                             <  1:2344759/101‑1 (MQ=255)
                                                                      cgcgcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcggcgc                           >  1:45748/1‑101 (MQ=255)
                                                                       gcgcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCATAAgcgcggcgcg                          <  1:2526115/101‑1 (MQ=255)
                                                                         gcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCgcggcgcggc                        >  1:1936310/1‑101 (MQ=255)
                                                                          cAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTGTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGcc                       <  2:754966/101‑1 (MQ=255)
                                                                          cAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGcc                       >  1:1449037/1‑101 (MQ=255)
                                                                          cAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGcc                       >  2:681765/1‑101 (MQ=255)
                                                                           aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCCCAAGCGCGGCGCGGccc                      >  2:1131859/1‑101 (MQ=255)
                                                                           aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGccc                      >  2:1026314/1‑101 (MQ=255)
                                                                           aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGccc                      <  2:3778447/101‑1 (MQ=255)
                                                                            agcccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCg                     <  2:2661944/99‑1 (MQ=255)
                                                                            aaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCg                     <  1:2179798/101‑1 (MQ=255)
                                                                             aCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCgg                    <  1:4462399/101‑1 (MQ=255)
                                                                               ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                  <  1:1166966/101‑1 (MQ=255)
                                                                                cGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATa                 <  1:3054288/101‑1 (MQ=255)
                                                                                    tgtTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGccc             >  1:3812579/1‑101 (MQ=255)
                                                                                     gtTGATGAAACCAAA.GGGGGTGCCCTGCCCCCCTCTGCCCCGGCCTTAGCCGATCACCATGTCGTCGCGATGCACCAGCGCGGCGCGGCCCGGATAGACca            >  2:33022/1‑101 (MQ=255)
                                                                                      ttGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcag           >  1:245957/1‑101 (MQ=255)
                                                                                       tgatgaAACCAAA.GGGGGGGCCCCGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc          <  2:834422/101‑1 (MQ=255)
                                                                                         atgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagcag        <  1:3468410/101‑1 (MQ=255)
                                                                                         atgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagcag        >  1:374151/1‑101 (MQ=255)
                                                                                          tgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCATAAGCGCGGCGCGGCCCGGATAGCCCagcagc       >  1:1501667/1‑101 (MQ=255)
                                                                                            aaaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCATAAGCGCGGCGCGGCCCGGATAGCCCAGCAgcgc     >  1:3967324/1‑101 (MQ=255)
                                                                                              aCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGccc   >  2:3292733/1‑100 (MQ=255)
                                                                                              aCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCt   >  1:3278093/1‑101 (MQ=255)
                                                                                               ccAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCCCAAGCGCGCCGCGGCCCGGATAGCCCAGCAGCCCCTc  >  2:4457244/1‑101 (MQ=255)
                                                                                               ccAAA.GGGGGGCCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTc  <  2:2283534/101‑1 (MQ=255)
                                                                                                    |                                                                                                
CCTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTC  >  NC_014034/2470153‑2470349

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG