Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,474,914	Δ1 bp	100%	intergenic (‑77/+49)	RCAP_rcc02301 ← / ← engA	hypothetical protein/GTP‑binding protein EngA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,474,914	0	G→.	100.0%	84.4	26	intergenic (‑77/+49)	RCAP_rcc02301/engA	hypothetical protein/GTP‑binding protein EngA
Reads supporting (aligned to +/- strand):  new base (20/5):  ref base (1/0):  total (21/5)

GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCCGCGGGTCGCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGC  >  NC_014034/2474814‑2475007                                                                                                     |                                                                                              gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                               <  1:1244641/101‑1 (MQ=255) gCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCg                                                                                               <  1:2037159/101‑1 (MQ=255)    cccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggg                                                                                            <  2:379642/101‑1 (MQ=255)    cccccGGGAT.GTTCCCCCAAAGGGGCCGGGAACCCCCGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggg                                                                                           <  2:2851798/101‑1 (MQ=255)     ccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggg                                                                                           <  2:1437575/101‑1 (MQ=255)      cccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggggg                                                                                          <  1:1860250/101‑1 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                         <  1:768270/101‑2 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                         <  1:3089540/101‑2 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                         <  1:4323882/101‑2 (MQ=255)       ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                         <  1:4343213/101‑2 (MQ=255)           gATATTTCCCCCAAGGTGACAGGAACCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAg                                                                                    <  1:3904676/101‑1 (MQ=255)           gATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAg                                                                                    <  2:3364361/101‑1 (MQ=255)           gATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAg                                                                                    >  2:2996598/1‑101 (MQ=255)            atatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGc                                                                                   <  2:1609158/101‑1 (MQ=255)             tatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcc                                                                                  <  2:2152260/101‑1 (MQ=255)             tatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcc                                                                                  <  2:2718800/101‑1 (MQ=255)             tatTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGCGGGGCggggggcgcggcc                                                                                   >  1:936895/1‑94 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCCAATACGCAAAGCGGGGGGGGC.GGGGGGCGCCGccc                                                                                 >  2:1679279/1‑101 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGG.GCAGccc                                                                                 >  2:1795079/1‑101 (MQ=255)              atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGGGC.GTGGGGCGCAGccc                                                                                 >  1:588686/1‑101 (MQ=255)                ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                               >  1:2976585/1‑101 (MQ=255)                  cccccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCcg                                                                             >  1:1195677/1‑101 (MQ=255)                  cccccAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGGGCGGGGGGG.GCAGccccccc                                                                             >  2:3498976/1‑100 (MQ=255)                        aGGTGACAGGAAACCGCGGCCCCCCCCACCCGAAGGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGTTGC.GGGGGGCGCAGCCCCCCGCggggc                                                                       >  1:2596793/1‑99 (MQ=255)                              cAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCggggccccccg                                                                 >  1:784385/1‑93 (MQ=37)                              cAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCCCggggtggcgcc                                                                 >  2:950855/1‑93 (MQ=37)                                     ccGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCGCCCCCGGGTTGc                                                          >  1:500718/1‑101 (MQ=25)                                       gcgGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCGCCCCGGGGTTTccc                                                        >  1:1318469/1‑101 (MQ=25)                                                                ggCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCGCCTCGGGGTTGCCCGCGGGGGCATCAGCCCCTCGGgttt                               >  1:2634824/1‑100 (MQ=21)                                                                        cTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCGCCTCGGGCCTGCCCCAGGCGACATCAGCCCCTCGGGTTCGAGGGCAg                       >  2:4559482/1‑101 (MQ=21)                                                                           ggCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCGCCTCGGGCCGGCCCCCGGGGACATCAGCCCCTCGGTTTCGGGGGGGGGcc                    >  1:3031755/1‑101 (MQ=18)                                                                               aaaTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCGCCTCGGGCTTGGCCGAGGCGACATCAGCCCTTCGGGTTCGAGGGCGGGCCCTTg                >  2:234391/1‑101 (MQ=25)                                                                                aaTACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGCCGCCCCGGGCGTGCCCGGGGGGGCACCAGCCCTTCCGTTTTGGGGGCGGGGCCGTGt               >  2:4256216/1‑101 (MQ=12)                                                                                       aaGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGAGCCTCGGGCCTGGCCGAGGCGACACCAGCCCTTCGGGTTCGAGGGCAGGACCTTGTCCTttcg        >  2:815171/1‑101 (MQ=18)                                                                                           cGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGTCCCCCCGGGCTTTCCCCAGGGGGCCTCAGCCCCTCGGTTTCGGGGGGAGGCCCCTGTCCtttggcggg    >  1:495413/1‑94 (MQ=12)                                                                                            ggggggTGC.GGGGGGCGCAGCCCCCCGCGGGTCGCCTCGGGGTTGCCCCAGGCGACAGCAGCCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTgcgg   >  2:3872753/1‑101 (MQ=25)                                                                                             gggggTGC.GGGGGGCGCAGCCCCCCGCGGGTCGCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTgcggc  >  1:2876307/1‑101 (MQ=255)                                                                                             gggggTGC.GGGGGGCGCAGCCCCCCGCGGGTCGCCCCCGGGGTGCCCCAGGCGACCTCAGCCCCTCGGTTTCGGGGGGAGGCCCCTGTCCTTGCGTgcggc  >  2:2491566/1‑101 (MQ=18)                                                                                             gggggTGC.GGGGGGCGCAGCCCCCCGCGGGGCGCCCCCGGCTTGCCCGGGGCGGCATCAGCCCCTCCGGTTCGAGGGCCGGCCCTTGTTCCCGCGGgcggc  >  1:2912582/1‑101 (MQ=12)                                                                                                     |                                                                                              GCTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCCGCGGGTCGCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGTTTCGAGGGCAGGCCCTTGTCCTTGCGTGCGGC  >  NC_014034/2474814‑2475007 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG