Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,493,971 Δ1 bp 3.1% intergenic (+19/‑223) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,493,9710G→. 3.1% 7.8 0.0 ‑0.0161intergenic (+19/‑223)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (42/114):  total (43/118)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.91e-01

Only 100 of 180 total aligned reads displayed.

TGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.G.GGGGG.G.CGCT.GCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAGCAG  >  NC_014034/2493876‑2494066
                                                                                                     |                                                                                               
ggATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCg                                                                                             <  2:1488038/100‑1 (MQ=39)
 gagggCCGAGCTGCCCACCCGCGCGCCGGGTCTGGCCCCGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGc                                                                                            <  1:1797970/98‑1 (MQ=38)
  aTGGCCGAGCGGCCCCCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCa                                                                                           <  2:3961752/101‑1 (MQ=38)
   tGGCCGAGCTGCCCCCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCaa                                                                                          <  1:883034/101‑1 (MQ=38)
   tGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCaa                                                                                          <  1:2791343/101‑1 (MQ=38)
   tGGCCGAGCTCCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCaa                                                                                          <  2:1690813/101‑1 (MQ=38)
    ggCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGGGTA.AGG.GGGGG.G.CGCG.GCCCCCCGTCCTCAAg                                                                                         >  2:1830487/1‑101 (MQ=255)
     gCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGc                                                                                        <  2:2633021/101‑1 (MQ=38)
     gCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGGCCGCAAGc                                                                                        >  1:960896/1‑101 (MQ=38)
        gAGCTGCCCCCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCggt                                                                                     <  1:898975/101‑2 (MQ=37)
        gAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCCAGCggc                                                                                     >  2:198472/1‑100 (MQ=38)
        gAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGGCCGCAAGccgc                                                                                     >  2:2545660/1‑98 (MQ=38)
          gCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACt                                                                                   <  2:1869229/101‑1 (MQ=37)
           cTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTc                                                                                  >  2:560328/1‑101 (MQ=37)
           cTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTc                                                                                  <  1:670319/101‑1 (MQ=37)
           cTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCCGCCGCAAGCGGCCTc                                                                                  >  1:4059277/1‑101 (MQ=38)
               ccACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTccccc                                                                              >  2:4223940/1‑101 (MQ=37)
               ccACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.GAGGGGG.G.GGCT.GGCCCCCGTCCGCAACCGGGCTccccc                                                                              >  1:3940115/1‑101 (MQ=255)
                 ccccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCg                                                                            <  2:4567270/100‑1 (MQ=25)
                 aCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GGCCCCCGTCCGCAAGCGGACTCCCCCCg                                                                            >  2:4339736/1‑101 (MQ=37)
                 aCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCg                                                                            >  2:3458920/1‑101 (MQ=37)
                 aCCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCACCCg                                                                            <  1:4392912/101‑1 (MQ=25)
                  cccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCgg                                                                           <  2:4030881/101‑1 (MQ=25)
                   ccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCAGGACAGGCGGAGTAGG.G.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCggg                                                                          <  2:1576482/101‑1 (MQ=25)
                     gcgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                                        <  2:2970912/101‑1 (MQ=25)
                      cgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.GGGT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGata                                                                       >  1:2243774/1‑101 (MQ=25)
                       gcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGatat                                                                      <  1:136867/101‑1 (MQ=25)
                        cgccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCCAGCGGACTCCCCCCGGGAAAtt                                                                     >  2:308257/1‑101 (MQ=25)
                         gccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATAttt                                                                    <  1:2828021/101‑1 (MQ=25)
                           cgcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgc                                                                  <  1:989608/101‑1 (MQ=25)
                            gcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCTCGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgca                                                                 <  1:4044647/101‑1 (MQ=21)
                            gcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.GGCT.GCCCCCCCTCCCCAAGCGGGGTCCCCCCCGGGGAttttcc                                                                 >  1:3429347/1‑98 (MQ=255)
                              gTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagc                                                               <  2:769952/101‑1 (MQ=25)
                               tCTGGCCCGGCGCCGCGGCGGTCGCGCGGCTCGACAGCCGGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                                              <  1:4519000/101‑1 (MQ=18)
                               tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                                              <  2:1373026/101‑1 (MQ=25)
                               tCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagca                                                              >  1:1264613/1‑101 (MQ=25)
                                 tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                                            <  1:4623226/101‑1 (MQ=21)
                                 tGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAg                                                            <  2:3276650/101‑1 (MQ=21)
                                  ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGa                                                           <  1:425134/101‑1 (MQ=21)
                                     ccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCTCCCGGGATATTTACAGCAAGATGa                                                        <  2:3203720/101‑1 (MQ=21)
                                     ccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGa                                                        <  2:1260855/101‑1 (MQ=21)
                                      cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                                       <  2:86021/101‑1 (MQ=21)
                                      cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.G.GGGGG.GCCGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                                       <  2:4418582/101‑1 (MQ=21)
                                       aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATNTTTGCAGGaannnnnnn                                                      >  2:453059/1‑94 (MQ=18)
                                       aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                      <  1:3263804/101‑1 (MQ=21)
                                         ctccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                    <  2:720224/99‑1 (MQ=18)
                                         cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAAcc                                                    >  2:3893179/1‑101 (MQ=21)
                                         cgccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAcc                                                    <  2:928915/101‑1 (MQ=21)
                                          gccgcGGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.GGCTCGCCCCCG.TCCGCAAGCGGGCTCCCCCCGCGATATGTTCAGGAAGGTGAAAcac                                                   >  2:3752442/1‑99 (MQ=16)
                                            cgcgGCGGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                 <  1:2415967/101‑1 (MQ=21)
                                            cgcgCCGGGCGCGCGCCCCGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                 <  2:4138218/101‑1 (MQ=17)
                                             gcggcgGGCGCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                <  1:3873785/101‑1 (MQ=21)
                                                gcgggcgCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                             <  2:3405661/101‑1 (MQ=21)
                                                 cgggcgCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                            <  1:576105/101‑1 (MQ=21)
                                                  gggcgCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                           <  2:3691524/101‑1 (MQ=21)
                                                   ggcgCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCGCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAACCGGATGCCttt                                          <  1:1624367/101‑1 (MQ=21)
                                                   ggcgCGCGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                          <  2:1947490/101‑1 (MQ=21)
                                                    gcgcgcGGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                         <  2:363510/101‑1 (MQ=21)
                                                       cgcgGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                      >  2:2974540/1‑101 (MQ=21)
                                                        gcgGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                     <  1:2334011/101‑1 (MQ=21)
                                                         cgGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                    <  1:2097046/101‑1 (MQ=21)
                                                         cgGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                    <  1:1966740/101‑1 (MQ=21)
                                                         cgGCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                    <  2:1020681/101‑1 (MQ=21)
                                                          ggCACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                   >  2:2736730/1‑101 (MQ=21)
                                                            cACGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGcc                                 >  2:1311081/1‑101 (MQ=21)
                                                              cGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                               <  1:2581620/101‑1 (MQ=21)
                                                              cGACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                               <  2:4362146/101‑1 (MQ=21)
                                                               gACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                              <  1:3525447/101‑1 (MQ=21)
                                                               gACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                              <  2:110926/101‑1 (MQ=21)
                                                               gACAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTTGCCGa                              >  2:1674435/1‑101 (MQ=17)
                                                                aCAGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                             <  2:4427230/101‑1 (MQ=21)
                                                                  aGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                           <  1:3816435/101‑1 (MQ=21)
                                                                  aGGCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                           <  2:647773/101‑1 (MQ=21)
                                                                   ggCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                          <  2:3559894/101‑1 (MQ=21)
                                                                   ggCCGAGTA.A.G.GGGGGCG.GTCG.GCCCCGCGCCCGCAAGGGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGCCCGAAata                          <  2:2306986/101‑1 (MQ=17)
                                                                    gCCGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                         <  2:1324367/101‑1 (MQ=21)
                                                                     tcGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                        <  1:4054669/100‑1 (MQ=18)
                                                                     ccGAGTA.GGG.GGGGG.G.CGCT.CCCCCCCGTCCGCAAGCGGACCCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                        <  2:2063019/101‑1 (MQ=18)
                                                                      cGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                       <  1:1686562/101‑1 (MQ=18)
                                                                      cGAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                       >  2:31263/1‑101 (MQ=18)
                                                                       gAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                      <  2:3429093/101‑1 (MQ=18)
                                                                       gAGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                      <  1:4192533/101‑1 (MQ=18)
                                                                        aGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGACATATCCCg                     >  2:704767/1‑101 (MQ=18)
                                                                        aGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                     <  1:1833890/101‑1 (MQ=18)
                                                                        aGTA.AGG.GGGGG.G.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                     >  1:1000152/1‑101 (MQ=18)
                                                                          gtaagg.ggggg.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                    >  1:1486152/6‑101 (MQ=255)
                                                                           taagg.ggggg.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                   >  1:3180596/5‑101 (MQ=18)
                                                                            aagg.ggggg.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                  >  1:1628290/4‑101 (MQ=255)
                                                                                 ggggg.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg              <  1:2172571/101‑1 (MQ=255)
                                                                                 ggggg.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg              <  1:4511415/101‑1 (MQ=255)
                                                                                  gggg.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa             >  1:3823557/1‑101 (MQ=255)
                                                                                  gggg.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa             <  2:2208328/101‑1 (MQ=255)
                                                                                     g.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATg          <  1:4340028/101‑1 (MQ=255)
                                                                                     g.g.CGCT.GCCCCCCGTCCGCAAGCGGACTCCCCCAGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATg          <  1:3232344/101‑1 (MQ=255)
                                                                                          gcT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCg       >  2:2159991/1‑101 (MQ=255)
                                                                                           cT.GCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGAAGCAAGATGAAACCGGATGCCTTTCATTTTGACCGAAATATCCCGGGGGGTGAATGGCGa      >  2:2703254/1‑101 (MQ=18)
                                                                                              gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGCTATTTGCAGCAAGATGCAACCGAATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGAGAAGGGGGaag    >  1:124217/1‑99 (MQ=18)
                                                                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaagcc  <  1:434851/101‑5 (MQ=255)
                                                                                               cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaagcc  <  1:589397/101‑5 (MQ=255)
                                                                                               cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaagc   <  1:198496/101‑4 (MQ=255)
                                                                                                     |                                                                                               
TGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCGAGTA.A.G.GGGGG.G.CGCT.GCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAGCAG  >  NC_014034/2493876‑2494066

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG