Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,022 Δ1 bp 6.8% intergenic (+70/‑172) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0220C→. 6.8% 22.1 ‑1.2 ‑0.5133intergenic (+70/‑172)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/3):  ref base (54/69):  total (61/72)
Fisher's exact test strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.14e-01

Only 100 of 143 total aligned reads displayed.

GGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGG.G.GCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCG  >  NC_014034/2493923‑2494120
                                                                                                      |                                                                                                        
 gcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                         <  2:3405661/101‑1 (MQ=21)
   gggcgCGCGGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                       <  1:4577252/101‑1 (MQ=21)
   gggcgCGCGGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                       <  2:3691524/101‑1 (MQ=21)
   gggcgCGCGGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                       <  2:3907789/101‑1 (MQ=21)
    ggcgCGCGGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                      <  2:1947490/101‑1 (MQ=21)
    ggcgCGCGGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                                                                                      <  2:399985/101‑1 (MQ=21)
     gcgcgcGGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                     <  1:4452450/101‑1 (MQ=21)
        cgcgGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                  >  2:2974540/1‑101 (MQ=21)
         gcgGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                 <  1:2334011/101‑1 (MQ=21)
          cgGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                                <  2:1020681/101‑1 (MQ=21)
          cgGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                                >  2:1188154/1‑101 (MQ=21)
          cgGCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                                <  1:1966740/101‑1 (MQ=21)
           ggCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                               >  2:2736730/1‑101 (MQ=21)
           ggCACGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                               >  2:1364262/1‑101 (MQ=21)
              aCGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATTTTGccc                                                                                            >  2:3578950/1‑101 (MQ=18)
               cGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                           <  2:4362146/101‑1 (MQ=21)
               cGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                           <  1:2581620/101‑1 (MQ=21)
               cGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                           <  1:1254986/101‑1 (MQ=21)
               cGACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                           <  1:2796169/101‑1 (MQ=21)
                gACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                          <  1:3525447/101‑1 (MQ=21)
                gACAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTTGCCGa                                                                                          >  2:1674435/1‑101 (MQ=17)
                 aCAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaa                                                                                         <  2:4427230/101‑1 (MQ=21)
                  cAGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                        <  2:1981220/101‑1 (MQ=21)
                   aGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                       <  1:3816435/101‑1 (MQ=21)
                   aGGCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                       <  2:647773/101‑1 (MQ=21)
                    ggCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                      <  2:3559894/101‑1 (MQ=21)
                    ggCCGAGTAA.GGGGG.GCGGTCGGCCCCGCGCCCGCAAGGGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCCTCTTGCCCGAAata                                                                                      <  2:2306986/101‑1 (MQ=17)
                     gCCGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                     <  2:1324367/101‑1 (MQ=21)
                      tcGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                    <  1:4054669/100‑1 (MQ=18)
                       cGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                   >  2:31263/1‑101 (MQ=18)
                       cGAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                   <  1:1686562/101‑1 (MQ=18)
                       cGAGTAAGGGGGG.G.GCCCTGCCCCCCGTCCGCAAGCCGACTCCCCCCGGCATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                   <  2:3151033/101‑1 (MQ=18)
                       agAGAAA.GGGGGAG.TCGCTGCCCCCCGTCCACAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                                                                                   <  1:530034/100‑1 (MQ=18)
                        gAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                  >  1:3418496/1‑101 (MQ=18)
                        gAGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                  <  2:3429093/101‑1 (MQ=18)
                         aGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                 <  1:1833890/101‑1 (MQ=18)
                         aGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                 >  1:1000152/1‑101 (MQ=18)
                         aGTAAGGGGGG.G.GCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAAGCATTTAATCTTGCCAGAAATATTCCg                                                                                 >  2:187457/1‑101 (MQ=17)
                          gtaagggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGGTATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                >  1:906249/6‑101 (MQ=17)
                          gtaagggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                >  1:1504263/6‑101 (MQ=255)
                          gtaagggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                >  1:1486152/6‑101 (MQ=255)
                          gtaagggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                <  1:1350477/96‑1 (MQ=255)
                           taagggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                               >  1:3180596/5‑101 (MQ=18)
                            aagggggg.g.gGCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                              <  2:1000069/98‑1 (MQ=18)
                            aagggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                              >  1:1628290/4‑101 (MQ=255)
                              gggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                            >  1:2629620/2‑101 (MQ=255)
                               ggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                           >  2:3028533/1‑100 (MQ=255)
                               ggggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                           <  2:960816/101‑1 (MQ=255)
                                gggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggggg                                                                          >  2:1621830/1‑99 (MQ=255)
                                gggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                          <  1:2172571/101‑1 (MQ=255)
                                gggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                          <  1:4511415/101‑1 (MQ=255)
                                 ggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                         >  1:3823557/1‑101 (MQ=255)
                                 ggg.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                         <  2:2208328/101‑1 (MQ=255)
                                   g.g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                      <  1:4515500/101‑1 (MQ=255)
                                     g.gCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                     <  1:4340028/101‑1 (MQ=255)
                                         gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                >  2:2159991/1‑101 (MQ=255)
                                          cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..G.a                                                              >  1:4349045/1‑101 (MQ=18)
                                           tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                             >  1:14024/1‑100 (MQ=255)
                                             cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                          <  2:1568902/101‑5 (MQ=255)
                                             cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                          >  1:422015/1‑97 (MQ=255)
                                             cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                          <  1:589397/101‑5 (MQ=255)
                                             cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                           <  1:198496/101‑4 (MQ=255)
                                              ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                         <  2:3670665/101‑6 (MQ=255)
                                                cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                        <  1:509831/97‑7 (MQ=255)
                                                 ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                       <  2:432097/98‑8 (MQ=255)
                                                  cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                      <  1:2418397/99‑9 (MQ=255)
                                                   gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                     >  2:4129599/2‑92 (MQ=255)
                                                         aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCa                                               <  1:814009/101‑1 (MQ=18)
                                                          aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCag                                              <  2:1264520/101‑2 (MQ=18)
                                                           gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATGTCAACGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGCagc                                             >  1:3989609/1‑99 (MQ=17)
                                                            cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcg                                            >  1:2943247/1‑98 (MQ=18)
                                                             ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCagcgc                                           >  1:798133/1‑97 (MQ=17)
                                                                cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccc                                      >  2:486759/1‑101 (MQ=18)
                                                                 tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCG.ccccc                                     <  2:1714876/101‑1 (MQ=17)
                                                                   cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccccct                                   <  2:2243612/101‑3 (MQ=17)
                                                                   cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.cccccct                                   <  1:2160141/101‑3 (MQ=17)
                                                                         ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                              >  1:1282178/1‑101 (MQ=2)
                                                                         ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcg                              >  2:4133702/1‑101 (MQ=2)
                                                                         ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcg                              >  2:4256564/1‑101 (MQ=17)
                                                                         ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCcg                              <  1:1726617/101‑1 (MQ=17)
                                                                          gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgc                             >  2:4290240/1‑100 (MQ=2)
                                                                           atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc                            <  1:1127028/101‑3 (MQ=2)
                                                                           atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgcc                            <  2:2444885/101‑3 (MQ=2)
                                                                            tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.CCCCCTGCCCcgccg                           <  2:2091493/101‑1 (MQ=2)
                                                                               ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCAGCGG.CCCCCGGCCCCGGCGGTc                        >  2:714330/1‑101 (MQ=18)
                                                                               ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTc                        <  1:1467624/101‑1 (MQ=17)
                                                                                tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                       >  1:3926664/1‑101 (MQ=18)
                                                                                 gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                      >  1:1658069/1‑101 (MQ=17)
                                                                                 gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCACAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCg                      >  1:4037011/1‑101 (MQ=18)
                                                                                  cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CCCCCCGCCCCCGCGGGcccgg                    >  1:4092509/1‑98 (MQ=17)
                                                                                  cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCACAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGt                     >  1:2256617/1‑101 (MQ=18)
                                                                                    gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAG.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                   >  2:294835/1‑101 (MQ=18)
                                                                                    gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTgg                   <  2:2629435/101‑1 (MQ=17)
                                                                                     caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggc                  >  2:2049065/1‑101 (MQ=18)
                                                                                     caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAGAGGGGGGCCGACC.CCCCCTGCCCCGGCGGTCCGCggg                  >  1:677081/1‑100 (MQ=17)
                                                                                              ccgggctGCCTTTAATCTTG.CCAAAACTTGCCGGGGGGGGAAGGGGC..GAAG.CGGGGGGGGGCCACCCC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg         <  2:2728849/95‑1 (MQ=255)
                                                                                                 ggATGCCTTTCATCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg      <  1:3085165/101‑1 (MQ=17)
                                                                                                  gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg     >  1:1199947/1‑101 (MQ=17)
                                                                                                    tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc   >  2:3668466/1‑101 (MQ=18)
                                                                                                     gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg  >  1:3532805/1‑101 (MQ=18)
                                                                                                      |                                                                                                        
GGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGG.G.GCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCG  >  NC_014034/2493923‑2494120

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG