Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,047 Δ1 bp 8.8% intergenic (+95/‑147) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0470G→. 8.8% 25.2 ‑0.3 ‑0.4114intergenic (+95/‑147)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/4):  ref base (56/48):  total (62/52)
Fisher's exact test strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.78e-01

Only 100 of 135 total aligned reads displayed.

AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146
                                                                                                    |                                                                                                           
aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGACATATCCCg                                                                                                             >  2:704767/1‑101 (MQ=18)
aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                                             >  1:1000152/1‑101 (MQ=18)
aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                                             <  1:1833890/101‑1 (MQ=18)
 gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                            <  1:1350477/96‑1 (MQ=255)
 gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                            >  1:1486152/6‑101 (MQ=255)
   aaggggggggGCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                          <  2:1000069/98‑1 (MQ=18)
   aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                          >  1:1628290/4‑101 (MQ=255)
      gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                                                       <  2:960816/101‑1 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggggg                                                                                                      >  2:1621830/1‑99 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                      <  1:1668650/101‑1 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                      <  1:4511415/101‑1 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                      <  1:2172571/101‑1 (MQ=255)
        gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                     <  2:2208328/101‑1 (MQ=255)
          gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                  <  1:4515500/101‑1 (MQ=255)
           ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                 <  1:4340028/101‑1 (MQ=255)
           ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCAGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                 <  1:3232344/101‑1 (MQ=255)
              gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                            >  2:2159991/1‑101 (MQ=255)
               cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..G.a                                                                                          >  1:4349045/1‑101 (MQ=18)
               cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGAAGCAAGATGAAACCGGATGCCTTTCATTTTGACCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                          >  2:2703254/1‑101 (MQ=18)
                 gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                        <  1:704842/101‑3 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                      >  1:422015/1‑97 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                      <  2:1568902/101‑5 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                      <  1:434851/101‑5 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                      <  1:589397/101‑5 (MQ=255)
                   ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                     <  2:3670665/101‑6 (MQ=255)
                     cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccag                                                                                    >  1:2985216/5‑95 (MQ=18)
                       cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                  <  1:2418397/99‑9 (MQ=255)
                        gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                 >  2:4129599/2‑92 (MQ=255)
                              aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCa                                                                          <  1:814009/101‑1 (MQ=18)
                               aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCag                                                                         <  2:1264520/101‑2 (MQ=18)
                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGG..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                  >  2:4255159/1‑101 (MQ=17)
                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                  >  2:2256315/1‑101 (MQ=18)
                                      tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.ccc.cc                                                               <  2:1714876/101‑1 (MQ=17)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccct                                                             <  2:2243612/101‑3 (MQ=17)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccct                                                             <  1:2160141/101‑3 (MQ=17)
                                         ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccctg                                                            <  2:2984987/101‑4 (MQ=17)
                                         ccccGGGATATCTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccctg                                                            >  1:2729047/1‑98 (MQ=17)
                                            cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCG.ccc.ccctgacc                                                         >  1:1814513/1‑95 (MQ=17)
                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCAC.CCC.CCTGcccc                                                         >  2:344576/1‑101 (MQ=17)
                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                        >  2:4133702/1‑101 (MQ=2)
                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                        >  1:1282178/1‑101 (MQ=2)
                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                        >  2:1903843/1‑101 (MQ=0)
                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcg                                                        >  1:991533/1‑101 (MQ=18)
                                              ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcg                                                        >  2:4256564/1‑101 (MQ=17)
                                               gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgc                                                       >  2:4290240/1‑100 (MQ=2)
                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                      >  1:3869648/1‑99 (MQ=0)
                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                      <  2:2444885/101‑3 (MQ=2)
                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                      <  1:1127028/101‑3 (MQ=2)
                                                atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcggc                                                      >  2:477874/1‑101 (MQ=17)
                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGT..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcggcg                                                     >  1:1457259/1‑101 (MQ=17)
                                                    ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCGG.CCC.CCGGCCCCGGCGGTc                                                  >  2:714330/1‑101 (MQ=18)
                                                    ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTc                                                  <  1:1467624/101‑1 (MQ=17)
                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                 <  2:1703597/101‑1 (MQ=17)
                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                 >  1:3926664/1‑101 (MQ=18)
                                                      gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCg                                                >  1:1658069/1‑101 (MQ=17)
                                                      gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCg                                                >  1:4037011/1‑101 (MQ=18)
                                                       cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGt                                               >  2:4340374/1‑101 (MQ=18)
                                                       cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGt                                               >  1:2256617/1‑101 (MQ=18)
                                                         gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAG.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg                                             >  2:294835/1‑101 (MQ=18)
                                                         gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg                                             <  2:2629435/101‑1 (MQ=17)
                                                          caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggc                                            >  2:2049065/1‑101 (MQ=18)
                                                          caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAG.AGGGGGGCCGACC.CCC.CCTGCCCCGGCGGTCCGCggg                                            >  1:677081/1‑100 (MQ=17)
                                                            aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGG.AAGCGCCCC.CCTGCCCCGGCGGTCCGGggcgg                                          >  1:1331463/1‑101 (MQ=17)
                                                               tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGgcgc                                       >  1:4604971/1‑101 (MQ=17)
                                                                 aaaCCGGATGCCTTTCATCTTCCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCt                                     <  2:3182635/101‑1 (MQ=17)
                                                                   ccgggctGCCTTTAATCTTG.CCAAAACTTGCCGGGGGGGGAAGGGGC..GAAG.CGGG.GGGGGGCCACCCC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                   <  2:2728849/95‑1 (MQ=255)
                                                                      ggATGCCTTTCATCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                <  1:3085165/101‑1 (MQ=17)
                                                                       gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                               >  1:1199947/1‑101 (MQ=17)
                                                                            cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                          <  2:2173145/101‑1 (MQ=17)
                                                                            cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                          <  2:3699520/101‑1 (MQ=17)
                                                                            cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                          >  1:4153737/1‑101 (MQ=18)
                                                                             tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         <  1:402025/101‑1 (MQ=17)
                                                                             tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         <  2:3939830/101‑1 (MQ=17)
                                                                             tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         <  1:2721423/101‑1 (MQ=17)
                                                                               tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                       <  2:3763269/101‑1 (MQ=18)
                                                                                cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCC.GCGGGGGCCAGCGCCCC.CCTGCCCCGCCGGTCCGTGGCGGGGCCTTGACGGGCggggggg                      >  2:349165/1‑99 (MQ=12)
                                                                                cATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..GAAG.TCAA.GGGGGGGCAGGCC.CCC.CCTAGCCAGGGCGTCCGGGGCGGGCCCTGGAGGGGGggggggg                      >  1:2574823/1‑99 (MQ=9)
                                                                                 aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                     <  1:31269/101‑1 (MQ=18)
                                                                                 aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                     >  1:3214311/1‑101 (MQ=18)
                                                                                  tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                    >  1:3698092/1‑101 (MQ=18)
                                                                                   cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCgg                   >  1:2097304/1‑101 (MQ=18)
                                                                                   cTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGGGCCTTGCCGCGCTGGGGCGCgg                   >  1:3664851/1‑101 (MQ=12)
                                                                                     tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGAGGCCCGTGGCGGCGCCTTGACGAGCGGGGGCGCGGGc                 >  2:1911068/1‑101 (MQ=17)
                                                                                     tGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCA.GAGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                >  1:3546810/1‑101 (MQ=21)
                                                                                        ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt              <  1:2906827/101‑1 (MQ=18)
                                                                                          gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCagatc            >  2:2489016/1‑97 (MQ=18)
                                                                                          gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa            <  1:2256477/101‑1 (MQ=18)
                                                                                          gAAATATCCCGGGGGGTGAA.TCGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa            <  1:4255447/101‑1 (MQ=18)
                                                                                          gAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCATCGC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGTGGGCGTGGGCATTaa            >  2:449851/1‑101 (MQ=21)
                                                                                          gAAATATCCCGGGGGGGGAA.AGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa            >  2:2097177/1‑101 (MQ=18)
                                                                                           aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc           >  1:1967380/1‑101 (MQ=18)
                                                                                            aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc          >  2:584359/1‑101 (MQ=21)
                                                                                              tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa        >  2:2484677/1‑101 (MQ=21)
                                                                                               atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGGATTAACCGaa       >  1:2619658/1‑101 (MQ=21)
                                                                                                tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa      <  1:867912/101‑1 (MQ=21)
                                                                                                tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa      <  2:14019/101‑1 (MQ=21)
                                                                                                 cccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt     >  1:890800/1‑101 (MQ=21)
                                                                                                   cGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg   <  1:3893464/101‑1 (MQ=25)
                                                                                                   cGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg   >  2:815951/1‑101 (MQ=25)
                                                                                                    ggggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCCACCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc  <  1:3245406/101‑1 (MQ=25)
                                                                                                    |                                                                                                           
AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG