Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,539,113 Δ1 bp 3.2% intergenic (‑106/+9) RCAP_rcc02366 ← / ← RCAP_rcc02367 NnrS family protein/calcium‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,539,1130G→. 3.2% 8.1 0.0 ‑0.5158intergenic (‑106/+9)RCAP_rcc02366/RCAP_rcc02367NnrS family protein/calcium‑binding protein
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (79/74):  total (82/76)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.10e-01

Only 100 of 201 total aligned reads displayed.

CTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCCGCGA  >  NC_014034/2539013‑2539213
                                                                                                    |                                                                                                     
cTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCg                                                                                                       <  2:3501983/101‑1 (MQ=255)
cTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCg                                                                                                       <  1:3558849/101‑1 (MQ=255)
 tCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGCGCAGGGGGCTTGCGGCGCCGTGCCgg                                                                                                      >  1:1551575/1‑101 (MQ=255)
 tCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCCGTCAAGCCGGGGATGTGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCgg                                                                                                      >  1:3862703/1‑101 (MQ=255)
 gccGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAGCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCgg                                                                                                      <  2:2848646/100‑1 (MQ=255)
   cGGCAGCGGGGATGTGCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCgggg                                                                                                    <  2:752892/101‑1 (MQ=255)
   cGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCgggg                                                                                                    >  1:1210426/1‑101 (MQ=255)
    ggCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCggggg                                                                                                   >  2:3660333/1‑101 (MQ=255)
     gCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGGGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCgggggg                                                                                                  >  1:1334317/1‑101 (MQ=255)
      cAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCggggggg                                                                                                 <  1:1258323/101‑1 (MQ=255)
         cGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCgggggggggg                                                                                              >  2:4261946/1‑100 (MQ=255)
          ggggGTGCCCGGGGGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGAATGCGCCAAACCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTc                                                                                             <  2:3424202/101‑1 (MQ=255)
          ggggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTc                                                                                             <  1:3645357/101‑1 (MQ=255)
          ggggATGTCCGGGGGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTc                                                                                             <  2:1295035/101‑1 (MQ=255)
           gggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCa                                                                                            >  1:1697966/1‑101 (MQ=255)
           gggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCa                                                                                            >  1:2466785/1‑101 (MQ=255)
            ggATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAg                                                                                           >  1:2846616/1‑101 (MQ=255)
              gtggccGGGTGGTGGGGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCGGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGTCAGCcg                                                                                        <  1:1051614/97‑1 (MQ=255)
              aTGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGcc                                                                                         >  2:4574699/1‑101 (MQ=255)
              aTGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGGCAGcc                                                                                         >  2:380804/1‑101 (MQ=255)
              aTGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGGCAGcc                                                                                         >  1:1717116/1‑101 (MQ=255)
               tggccGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCcg                                                                                        <  2:1966687/98‑1 (MQ=255)
               tggccGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCcg                                                                                        <  1:1004484/98‑1 (MQ=255)
                gTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCcgc                                                                                       >  2:1440846/1‑101 (MQ=255)
                  ccGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCgc.gg                                                                                    <  1:3517375/101‑1 (MQ=255)
                    gggTGGTGGCGCCGGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCgc.ggcg                                                                                  <  1:4618689/101‑1 (MQ=255)
                    gggTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGGCAGCCgc.ggcg                                                                                  >  2:4046284/1‑101 (MQ=255)
                    gggTGGGGGCGCCCGGCCGCCGGGCTGTCAAGGGGGGGGTGCGCCAAAACCCCGGGGCAGGGGGCTTGCGGGGCCGCGGCGGGGGGGGGGCAggaccgggg                                                                                   >  2:569051/1‑93 (MQ=255)
                      gtggtgGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCgc.ggcggc                                                                                >  1:1100564/1‑101 (MQ=255)
                      gtggtgGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCgc.ggcggc                                                                                >  1:3387937/1‑101 (MQ=255)
                      gtggggGCGCCGGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAACCCCCGGTGCGGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCgc.ggcggc                                                                                <  1:14144/101‑1 (MQ=255)
                       cttgacagcCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCCGCCgc.ggcggcg                                                                               >  2:2023637/8‑101 (MQ=255)
                        ggtggCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGgcgc                                                                              >  1:665794/1‑101 (MQ=255)
                        ggtggCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGgcgc                                                                              <  1:2777810/101‑1 (MQ=255)
                          tggCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCgg                                                                            >  2:1952512/1‑101 (MQ=255)
                          tggCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCgg                                                                            >  1:50663/1‑101 (MQ=255)
                            gcgcCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTc                                                                          <  2:3552013/101‑1 (MQ=255)
                              gcCCGGCCGCCGGGCTGTCAAGGTGGGGATGCCCCAAAACCCCGGGGCAGGGGGCTTGCGGCGCCGGGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCg                                                                        <  1:3418437/101‑1 (MQ=255)
                                ccGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGaa                                                                      <  1:3185868/101‑1 (MQ=255)
                                ccGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGGCAGCCGC.GGCGGCGCGGTCCGaa                                                                      >  2:3719984/1‑101 (MQ=255)
                                  ggCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAAt                                                                    <  2:2292992/101‑1 (MQ=255)
                                   gccgccGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCg                                                                  <  1:98084/101‑1 (MQ=255)
                                     cgccgGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCgg                                                                 >  1:2046588/1‑101 (MQ=255)
                                      gccgGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGc                                                                >  1:3993723/1‑101 (MQ=255)
                                      gccgGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGc                                                                >  2:2498196/1‑101 (MQ=255)
                                        cgGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCGGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCa                                                              <  1:241878/101‑1 (MQ=255)
                                          ggCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGc                                                            >  1:749715/1‑101 (MQ=255)
                                          ggCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGc                                                            >  2:3471797/1‑101 (MQ=255)
                                             tGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTc                                                         >  1:1607533/1‑101 (MQ=255)
                                              gTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTca                                                        <  2:1210334/101‑1 (MQ=255)
                                                cAAGGTGGGGATGCGCCAAACCCCCGCTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTcacc                                                      <  2:1531280/101‑1 (MQ=255)
                                                cAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGCCAGCCGC.GGCCGCGCGGTCCGAAATCGGCCAGGGGcacc                                                      >  1:4593103/1‑101 (MQ=255)
                                                 aaGGTGGGGATGCGCCAAACCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTcacca                                                     <  2:3904954/101‑1 (MQ=255)
                                                 aaGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTcacca                                                     <  2:590560/101‑1 (MQ=255)
                                                 aaGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGGCAGCCGC.GGGGGGGCGGGCCGAAAACGGCCAGCGGcacca                                                     >  1:1603108/1‑101 (MQ=255)
                                                 aaGGTGGGGATGCGCAAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGG.CAACCGCGGGGGGGGCGGCCCGAAACGGGCCAGGGCcaccc                                                     >  1:3933784/1‑100 (MQ=255)
                                                    gTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCCCGc                                                  >  2:4424311/1‑101 (MQ=255)
                                                     gggggATGCGCCAAACCCCCGGTGCAGGGGGCTGGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGcc                                                 <  1:1080929/100‑1 (MQ=255)
                                                      ggggATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCg                                                >  2:1246934/1‑101 (MQ=255)
                                                       gggATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCgc                                               >  1:4349947/1‑100 (MQ=255)
                                                       gggATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGt                                               >  2:2489557/1‑101 (MQ=255)
                                                       gggATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCC.GGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTc                                              >  1:3758806/1‑101 (MQ=255)
                                                         gATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGCCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCg                                             <  1:3999968/101‑1 (MQ=255)
                                                           tGCGCCAAAACCCGGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGcc                                           <  2:2941538/101‑1 (MQ=255)
                                                           tGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGcc                                           <  1:3125663/101‑1 (MQ=255)
                                                             cgcCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCgg                                         <  2:1641036/101‑1 (MQ=255)
                                                                cAAAACCCCGGTGCGGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGc                                      <  1:2207121/101‑1 (MQ=255)
                                                                cAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGc                                      >  1:1300233/1‑101 (MQ=255)
                                                                 aaaaCCCCGGTGCGGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCg                                     <  2:3956826/101‑1 (MQ=255)
                                                                 aaaaCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCg                                     <  1:76138/101‑1 (MQ=255)
                                                                  aaaCCCCGGTGCGGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCgg                                    <  2:95206/101‑1 (MQ=255)
                                                                    aCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGGGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTc                                  <  1:4542649/101‑1 (MQ=255)
                                                                    aCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTc                                  >  1:690758/1‑101 (MQ=255)
                                                                    aCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTc                                  <  2:2624549/101‑1 (MQ=255)
                                                                    aCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTc                                  >  2:2886925/1‑101 (MQ=255)
                                                                    aCCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTc                                  <  1:1889317/101‑1 (MQ=255)
                                                                       ccGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGGGCGGTcgcg                               >  2:2398741/1‑101 (MQ=255)
                                                                           tGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATc                           >  1:4465128/1‑101 (MQ=255)
                                                                           tGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGGCACCACGCCGTCGCCGGGGCGGTCGCGCATc                           >  1:865070/1‑101 (MQ=255)
                                                                            gCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCg                          >  1:1365946/1‑101 (MQ=255)
                                                                             cAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGAGGTCGCGCATCGc                         >  2:708937/1‑101 (MQ=255)
                                                                                ggggCTTGCGGCGCGGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCaaa                      <  2:2466619/101‑1 (MQ=255)
                                                                                 gggCTTGCGGCGCGGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGCCGCCGGTGCGGTCGCGCATCGCAAAc                     >  2:3771654/1‑101 (MQ=255)
                                                                                  ggCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAAcc                    >  2:2251494/1‑101 (MQ=255)
                                                                                  ggCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAAcc                    <  1:4171025/101‑1 (MQ=255)
                                                                                     ttGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGc                 >  2:2696858/1‑101 (MQ=255)
                                                                                      tGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCg                <  1:3072499/101‑1 (MQ=255)
                                                                                      tGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCg                <  1:181215/101‑1 (MQ=255)
                                                                                      tGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCg                >  2:2346414/1‑101 (MQ=255)
                                                                                       gcggcgCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCCTCGCAAACCAGCGa               >  2:3204858/1‑101 (MQ=255)
                                                                                       gcggcgCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGa               >  2:2078778/1‑101 (MQ=255)
                                                                                        cggcgCCGGGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGAt              <  2:665732/101‑1 (MQ=255)
                                                                                               gggcggggggggggTCAGCCGC.GGAGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGcc       <  1:1432035/96‑1 (MQ=255)
                                                                                               gTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGcc       >  1:970498/1‑101 (MQ=255)
                                                                                                 gCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCcg     >  2:1370158/1‑101 (MQ=255)
                                                                                                 gCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCcg     >  1:2978626/1‑101 (MQ=255)
                                                                                                  ccGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCcgc    <  1:380841/101‑1 (MQ=255)
                                                                                                  ccGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCcgc    <  1:4374017/101‑1 (MQ=255)
                                                                                                  ccGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCcgc    >  2:2844406/1‑101 (MQ=255)
                                                                                                    gggggggggTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCCGcga  >  1:1962955/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CTCCGGCAGCGGGGATGTCCGGGTGGTGGCGCCCGGCCGCCGGGCTGTCAAGGTGGGGATGCGCCAAAACCCCGGTGCAGGGGGCTTGCGGCGCCGTGCCGGGGGGGGGTCAGCCGC.GGCGGCGCGGTCCGAAATCGGCCAGCGTCACCACGCCGTCGCCGGTGCGGTCGCGCATCGCAAACCAGCGATCGCTGCCCGCGA  >  NC_014034/2539013‑2539213

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG