Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,559,935 Δ1 bp 100% intergenic (‑211/+59) ispZ ← / ← ftsY intracellular septation protein A/cell division protein FtsY

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,559,9350C→.100.0% 21.8 123intergenic (‑211/+59)ispZ/ftsYintracellular septation protein A/cell division protein FtsY
Reads supporting (aligned to +/- strand):  new base (105/9):  ref base (1/8):  total (106/17)
Fisher's exact test strand distribution p-value = 1.98e-07
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.85e-03

Only 100 of 158 total aligned reads displayed.

GGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGGGGGGGGCAGCGCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTCC  >  NC_014034/2559836‑2560033
                                                                                                     |                                                                                                  
  tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                                 >  2:2950554/1‑101 (MQ=255)
  tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                                 >  1:1407250/1‑101 (MQ=255)
  tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                                 >  1:3362986/1‑101 (MQ=255)
    tCCTGACAGGATTGTGTGCTTCGGGCCGCCGCAGGCACCGC.TTTCCTGGGGCGAACGGTCTGGGTTGTG.CCCGTCAGCTCACTTCGGCGGCAGCGccctcc                                                                                               >  2:763671/1‑101 (MQ=255)
    tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCGcccccc                                                                                               >  2:2766016/1‑101 (MQ=255)
      cTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccga                                                                                             >  2:860099/1‑99 (MQ=255)
       tGACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccgaa                                                                                            <  2:2762673/101‑4 (MQ=255)
        gACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCGccccccgaag                                                                                           >  1:3325473/1‑97 (MQ=255)
          cAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCANCGCAAGCGGGGGGCAGCG.CCCCCCGAAgcg                                                                                        >  1:1459533/1‑101 (MQ=255)
          cAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAgcg                                                                                        >  2:131257/1‑101 (MQ=255)
            ggtttGTCTCCCCCGCGCCGCGGCGGCC.CCTTTTTTCTGGGGGCGAAGCGCGGGGGGTTGG.CGCGCCAGCGCAGGGGGGGGGCGGC.CCCCCCCGAAGCGcc                                                                                      <  1:2100771/98‑1 (MQ=255)
            ggATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGcc                                                                                      >  2:4201112/1‑101 (MQ=255)
             gATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCt                                                                                     >  1:2327164/1‑101 (MQ=255)
              aTTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTg                                                                                    >  1:4176260/1‑101 (MQ=255)
              aTTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTg                                                                                    >  2:1958746/1‑101 (MQ=255)
               ttGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGa                                                                                   >  1:2514673/1‑101 (MQ=255)
                tgtgTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGGTGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAg                                                                                  >  2:4033878/1‑101 (MQ=255)
                tgtgTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAg                                                                                  >  1:2751928/1‑101 (MQ=255)
                 gtgtCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAgg                                                                                 >  2:2486723/1‑101 (MQ=255)
                 gtgtCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAgg                                                                                 >  1:800156/1‑101 (MQ=255)
                   gtCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGgcg                                                                               >  2:4342275/1‑101 (MQ=255)
                   gtCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGgcg                                                                               >  2:3819735/1‑101 (MQ=255)
                      cTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcgg                                                                            >  1:2408755/1‑101 (MQ=255)
                      cTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcgg                                                                            >  2:1741266/1‑101 (MQ=255)
                       ttCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcggc                                                                           >  2:4268518/1‑101 (MQ=255)
                       ttCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcggc                                                                           >  1:1205637/1‑101 (MQ=255)
                        tCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCgcggcg                                                                          >  2:4581061/1‑101 (MQ=255)
                         cgcgCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGcggcgg                                                                         >  2:3825825/1‑101 (MQ=255)
                              cgcAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGGTTg                                                                    >  2:628918/1‑101 (MQ=255)
                              cgcAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTg                                                                    >  2:2008478/1‑101 (MQ=255)
                                cagcagGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcc                                                                  >  2:4405362/1‑101 (MQ=255)
                                 agcagGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgccc                                                                 >  2:2034485/1‑101 (MQ=255)
                                   cagGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                               >  2:3921153/1‑101 (MQ=255)
                                   cagGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                               >  2:3664354/1‑101 (MQ=255)
                                         ccGTTTTCCCTGGGGCGAACCGCGGGGGGGTGG.CGCGCCGGCGCAGGGGGGGGGC.GCCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAAc                                                          <  1:2083477/101‑1 (MQ=21)
                                          cGCTTTTCGTGGGGCGAACCG.CGGGGGGTTGCCGCGTCCGCTCAGGCGGGGGCCAGC.CCCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACg                                                         <  2:1442397/101‑1 (MQ=16)
                                           gtttttCCTGGGGCGAAACGCGGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCGGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGa                                                        <  2:800082/99‑1 (MQ=37)
                                              tttCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGGCCGGCCAACGAAgg                                                     >  1:671028/1‑101 (MQ=255)
                                              tttCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCCTGTCCGCCCAACGAAgg                                                     >  2:3191725/1‑101 (MQ=255)
                                                tgcgggggGGAAGCGCGGGGGGGGGG.CGGGGCGGGGCAGGGGGGGGGGGGCCCCCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgc                                                    <  2:1142709/96‑1 (MQ=12)
                                                 ccTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgcgc                                                  >  1:4643917/1‑101 (MQ=255)
                                                 ccTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGgcgc                                                  >  1:2554240/1‑101 (MQ=255)
                                                  cTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCa                                                 >  1:3147630/1‑101 (MQ=255)
                                                         gAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGGTTGCCCGCCCCACGAAGGCGCCacccccc                                          >  1:926884/1‑100 (MQ=25)
                                                         gAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                                          >  2:706856/1‑101 (MQ=255)
                                                         gAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                                          >  1:433332/1‑101 (MQ=255)
                                                          aaccGCGGGGGGGTGG.CGCGTCAGCGCAGGGGGGGGGC.CCCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACAACCtt                                         <  1:3086737/98‑1 (MQ=21)
                                                          aaatcccGGGGCGTTG.GGCTTCCGCGTCAGGGGGGGGTCGGGCCCCCCGAAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                                          <  2:4593334/96‑1 (MQ=16)
                                                          aaaCGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCCACCACCtt                                         >  2:4534255/1‑101 (MQ=255)
                                                           aaCGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCCCCTTc                                        >  1:2348689/1‑101 (MQ=255)
                                                            ccGCGGGGGGGTGG.CGCGCGACCGCAAGGGGGGGACCGCG.CCCCCCAAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCa                                       <  2:544583/100‑1 (MQ=21)
                                                             cGCGGGGGGGTTG.GGCGTCAGCGAAGGGGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                                      <  2:1289603/101‑1 (MQ=25)
                                                             cGCCGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                                      >  2:2308527/1‑101 (MQ=255)
                                                             cGCCGGGGGGTGG.CGGGGCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAg                                      <  2:3068947/101‑1 (MQ=25)
                                                               ccGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTc                                    >  1:4474095/1‑101 (MQ=255)
                                                                cGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCCCCTTCAGTCg                                   >  1:4075753/1‑101 (MQ=255)
                                                                cGGGGGGTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                                   <  2:3148960/101‑1 (MQ=255)
                                                                 ggggggTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCTTCCGTCgg                                  >  2:3992547/1‑101 (MQ=25)
                                                                 ggggggTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCTTCAGTCgg                                  >  1:3284946/1‑101 (MQ=255)
                                                                 ggggggTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                                  <  2:2327002/101‑1 (MQ=255)
                                                                  gggggTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGc                                 >  2:496756/1‑101 (MQ=255)
                                                                    gggTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCtt                               >  1:4654188/1‑101 (MQ=255)
                                                                     ggTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGGGCAACCCCCTTCAGTCGGCTTc                              >  2:2601180/1‑101 (MQ=25)
                                                                     ggTTG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTc                              <  2:2202056/101‑1 (MQ=255)
                                                                       ttG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCgg                            >  2:1064051/1‑100 (MQ=255)
                                                                        tG.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGAt                           >  2:2716253/1‑101 (MQ=255)
                                                                         g.cgcGTCAGCGCGAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATc                          >  1:2526650/1‑101 (MQ=255)
                                                                         g.cgcGTCAGCGCAAGCGGGGGGCCGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGACGGCGCACCCACCCTCAGTCGGCTTCGATc                          >  2:794533/1‑101 (MQ=25)
                                                                            gcgTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATccc                        >  1:626912/1‑101 (MQ=255)
                                                                            gcgTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATccc                        >  1:1712733/1‑101 (MQ=255)
                                                                             ggaccgcggAAGGGGGGGGC.CCCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcccc                       <  1:1311214/96‑1 (MQ=25)
                                                                             cgTCAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCCTCGATcccc                       >  1:2943833/1‑101 (MQ=255)
                                                                                cAGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacc                    >  1:1384595/1‑101 (MQ=255)
                                                                                 aGCGCAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacca                   >  1:3869606/1‑101 (MQ=255)
                                                                                  gcgcAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAg                  >  1:4420977/1‑101 (MQ=255)
                                                                                   cgcAGGGGGGGGGCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgc                 <  2:348351/101‑1 (MQ=255)
                                                                                   cgcAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAAAAACCTTCCGTCGGCTTCGATCCCCCCCAgc                 >  1:293865/1‑101 (MQ=21)
                                                                                   cgcAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCCCCAgc                 >  1:1289894/1‑101 (MQ=255)
                                                                                   cgaaaGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgc                 <  2:559510/98‑1 (MQ=255)
                                                                                    gcAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcg                >  1:491704/1‑101 (MQ=255)
                                                                                    gaaaGGGGGGGGCAGCG.CCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcg                <  2:515323/99‑1 (MQ=255)
                                                                                    ccagggggggggCCCCCCCCCCCGAAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgc                 <  2:3515937/97‑1 (MQ=25)
                                                                                     cAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTTGATCCCCACCAgcgc               >  2:3737991/1‑101 (MQ=255)
                                                                                     cAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgc               >  2:3068074/1‑101 (MQ=255)
                                                                                     cAAGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAAAGAAGGCGCAACCACCTTCAGTCGGCTTCCATCCccaccacccc               >  2:2131334/1‑97 (MQ=21)
                                                                                     cAAGCGGGGGCCCGCG.CCCCCGGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgc               <  1:692440/101‑1 (MQ=25)
                                                                                      aaGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTGGGCTTAGATACCCACCAGagcg              >  2:4416985/1‑101 (MQ=21)
                                                                                      aaGCGGGGGGCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGcgcg              >  1:3057170/1‑101 (MQ=255)
                                                                                        ggggggggC.GCCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggc            <  1:3446787/101‑1 (MQ=255)
                                                                                         gggggggCGGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcg           <  2:2657518/101‑1 (MQ=255)
                                                                                         gggggggCCGCCCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggc            <  2:2554066/101‑1 (MQ=255)
                                                                                         cggggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGGGGGCTTGCCCGCCCAACGAAGGCGCAACCCCCTTCAGGCGGCTTCGATCCCCACCAACccggcg           >  1:1111805/2‑101 (MQ=255)
                                                                                          ggggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTNANNNGGCTTCGATCCCCACCAGCgcggcgg          >  1:2598157/1‑101 (MQ=255)
                                                                                          ggggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCCCCCAACGAAGGCGCAACCACCCTCAGTCGGCTTCGATCCCCACCCGCccggcgg          >  2:1688366/1‑101 (MQ=25)
                                                                                           ggggcCAGC.CCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcggc         <  1:605723/101‑1 (MQ=255)
                                                                                            ggggcgccccccccccAAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcggc         <  1:2573121/92‑1 (MQ=37)
                                                                                            ggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCCTCAGTCGGCTTCGATCCCCACCAGCgcggcggcg        >  1:1749652/1‑101 (MQ=255)
                                                                                            ggggCAGCG.CCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCgcggcggcg        >  1:2807640/1‑101 (MQ=255)
                                                                                              gggccgcccccccaGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGa       <  1:3821976/94‑1 (MQ=255)
                                                                                                   gccccccccGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTcc  <  1:4201400/99‑1 (MQ=255)
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GGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGC.CCGCTTTTCCTTGGGCGAAACGCCGGGGGGTTG.CGCGTCAGCGCAAGGGGGGGGCAGCGCCCCCCCGAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCGGCGGCGAATTCC  >  NC_014034/2559836‑2560033

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG