BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,640,201	Δ1 bp	100%	intergenic (‑177/+99)	thrS ← / ← RCAP_rcc02462	threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,640,201	0	A→.	100.0%	144.1	146	intergenic (‑177/+99)	thrS/RCAP_rcc02462	threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand): new base (62/51): ref base (15/18): total (77/69)
Fisher's exact test strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.32e-05

Only 100 of 158 total aligned reads displayed.


AGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.G.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640102‑2640297


aGGCGTATCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCGCCCCTCTT.G.GCC.TTCGGCCATTTCACCCCCCGAGGTACTTGGAGCAAGACGa                                                                                                    <  1:2659316/101‑1 (MQ=25)


 ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCCCTT.G.G.CGCTTGGGCCATTTACCCCCCCAGGTATTTGGAGGAAGATGaa                                                                                                   >  1:2089534/1‑101 (MQ=255)


   cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCTCTTCT.C.C.TTCGGCCAATTCACCCCCCCCGGTATTTTGAGCAAGATGaaaa                                                                                                 >  2:1682965/1‑101 (MQ=25)


      gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                              <  1:1109101/101‑5 (MQ=25)


        catccGTGGCGCTTTTGCGCACGCCATATCGGGGCGCAGCGCCCCTCTT.GCA.C.TTAGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATGaaatgccat                                                                                            <  2:2492458/98‑7 (MQ=255)


         aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                          <  2:355009/101‑1 (MQ=21)


          aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..g                                                                                       <  1:1652240/101‑1 (MQ=25)


           ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg..gg                                                                                      <  1:401648/101‑1 (MQ=25)


              tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTTCG.T.C.TTTGGCCAATTCAGCCTCCGAGGTATTTTTGCCAAGATG.CAAGAGCATG..GGTTc                                                                                   >  2:3141611/1‑101 (MQ=16)


              tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTc                                                                                   <  2:553602/101‑1 (MQ=21)


              tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTc                                                                                   <  1:2114179/101‑1 (MQ=21)


               ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCa                                                                                  <  2:2789973/101‑1 (MQ=25)


                gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCAt                                                                                 <  2:898430/101‑1 (MQ=25)


                 cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCATCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATc                                                                                >  1:1989832/1‑101 (MQ=21)


                    ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTg                                                                             <  2:2517352/101‑1 (MQ=25)


                        gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                         <  1:2311517/101‑1 (MQ=21)


                           gccccgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAat                                                                     <  2:2641040/95‑1 (MQ=14)


                            agccAAAAGGGGGCCGCTGCCCCCCTCTT.G.GTC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAat                                                                     <  2:746158/98‑1 (MQ=25)


                             ggCAAAAGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAata                                                                    <  2:3575984/101‑1 (MQ=21)


                             cccgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAatat                                                                   <  2:2473130/97‑1 (MQ=17)


                               cgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                                                 <  1:4234149/99‑1 (MQ=17)


                               cgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                                                 <  2:575675/99‑1 (MQ=17)


                               cgaaaAGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                                                 >  2:4119652/3‑101 (MQ=17)


                                gaaaAGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATccc                                                                <  1:1215644/100‑1 (MQ=17)


                                 aaaGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                                <  2:3189647/101‑1 (MQ=21)


                                 aaaGGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                                <  1:518442/101‑1 (MQ=25)


                                 aaaAGGGGGCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                               <  2:2748614/101‑1 (MQ=17)


                                  aaagggggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:3705510/4‑101 (MQ=17)


                                    agggggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                            >  1:1356112/2‑101 (MQ=18)


                                     gggggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggg                                                            <  2:2731094/101‑1 (MQ=25)


                                     gggggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggg                                                            <  2:1352989/101‑1 (MQ=25)


                                     gggggCGCTG.CCCCCTCTT.G.GCC.TTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggg                                                          <  2:2120767/101‑1 (MQ=2)


                                       gggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACGCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggggg                                                          <  2:2321714/101‑1 (MQ=25)


                                       gggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                         <  2:2966740/101‑3 (MQ=18)


                                       gggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                         >  1:4408096/1‑99 (MQ=18)


                                       gggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                         >  2:3699403/1‑99 (MQ=18)


                                        ggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGG..GGTT.ATCTTGCCCCAAATATCCCggggggga                                                        >  2:2151925/1‑99 (MQ=18)


                                        ggCGCTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                         <  2:1026362/101‑1 (MQ=25)


                                        gccgcTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                                                        <  1:1084325/99‑4 (MQ=21)


                                          cgcTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCGCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                      <  2:3939473/101‑6 (MQ=21)


                                          cgcTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                      <  2:240521/101‑6 (MQ=21)


                                           gcTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                     >  1:3459872/1‑95 (MQ=21)


                                            cTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGGTATTTGCGGCAAGGGGAAAGGA.TGGG..GGTT.TTCTTGCCCCCAATATCCCgggggggaggcg                                                    >  1:1756701/1‑95 (MQ=18)


                                            cTGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                    >  1:2889601/1‑94 (MQ=25)


                                             tGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGCGGTATTTTGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCCAATATCCCGGGGGGGGGCCt                                                    >  2:1749875/1‑101 (MQ=21)


                                             tGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcca                                                    >  2:2032427/1‑100 (MQ=25)


                                             tGCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                    <  1:3683008/101‑1 (MQ=25)


                                              gCCCCCCTCTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggggaggcgaa                                                  >  1:127641/1‑93 (MQ=25)


                                               ccccccTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                                  >  2:1899673/1‑101 (MQ=25)


                                               ccccccTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                                  >  2:1541300/1‑101 (MQ=25)


                                                  cccTCTT.G.GCC.TTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGTCTCAAATATCCCGGGGGGAGTCCTGAAAg                                               <  1:3644658/101‑1 (MQ=25)


                                                  cccTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                               >  1:2889481/1‑101 (MQ=37)


                                                  cccTCTT.G.GAC.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                               <  1:2032281/101‑1 (MQ=37)


                                                   ccTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              >  1:462203/1‑101 (MQ=37)


                                                   ccTCTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              <  1:3501361/101‑1 (MQ=37)


                                                    ctctT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             >  2:3327683/1‑101 (MQ=37)


                                                    ctctT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             >  1:3527331/1‑101 (MQ=37)


                                                     tctT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                            <  1:1242583/101‑1 (MQ=37)


                                                          tggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  2:1307922/5‑101 (MQ=37)


                                                            gcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                       <  2:1989697/99‑1 (MQ=37)


                                                              c.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     >  1:4438987/1‑101 (MQ=37)


                                                                ttCGGACAAGTCACCCCCCGAGGTATTTGGGGCAAGATGAAAA.ACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                    <  2:1841463/101‑1 (MQ=25)


                                                                 tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                   <  1:4496931/101‑1 (MQ=255)


                                                                   ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                 <  1:1063811/101‑1 (MQ=255)


                                                                    gCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                <  1:3351117/101‑1 (MQ=255)


                                                                    gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                >  2:158525/1‑101 (MQ=37)


                                                                     ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                               >  1:2359969/1‑101 (MQ=37)


                                                                        aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            >  1:4191721/1‑101 (MQ=255)


                                                                         ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           <  2:1371737/101‑1 (MQ=255)


                                                                          tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          >  1:914169/4‑101 (MQ=25)


                                                                           caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                         <  2:4109499/99‑1 (MQ=255)


                                                                           caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                         >  1:1358911/3‑101 (MQ=255)


                                                                            cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                        <  2:2067508/100‑1 (MQ=255)


                                                                             cccaccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       <  1:2319124/101‑1 (MQ=255)


                                                                               ccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                     >  2:2959146/1‑101 (MQ=255)


                                                                                 ccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                   >  1:1167436/1‑101 (MQ=255)


                                                                                 ccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                   >  2:426744/1‑101 (MQ=255)


                                                                                 ccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                   >  1:3175284/1‑101 (MQ=255)


                                                                                   gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 >  2:34989/1‑101 (MQ=255)


                                                                                   gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCt                 >  2:1794586/1‑101 (MQ=255)


                                                                                    aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                >  1:4329800/1‑101 (MQ=255)


                                                                                    aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                >  1:1691652/1‑101 (MQ=255)


                                                                                    aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                >  2:3117512/1‑101 (MQ=255)


                                                                                    aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                >  1:2481085/1‑101 (MQ=255)


                                                                                       tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:3314646/1‑101 (MQ=255)


                                                                                       tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:756393/1‑101 (MQ=255)


                                                                                       tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:4578592/1‑101 (MQ=255)


                                                                                         tttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           >  2:3242799/1‑101 (MQ=255)


                                                                                           tGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         >  1:2135655/1‑101 (MQ=255)


                                                                                           tGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         <  1:2606822/101‑1 (MQ=255)


                                                                                            ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  2:1075966/1‑101 (MQ=255)


                                                                                            ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  1:1476741/1‑101 (MQ=255)


                                                                                            ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  2:2639042/1‑101 (MQ=255)


                                                                                             gagcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg       >  1:3223233/4‑101 (MQ=255)


                                                                                             gagcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg       >  2:743558/4‑101 (MQ=255)


                                                                                              agcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt      >  1:2821026/3‑101 (MQ=255)


                                                                                                cAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  2:3984726/1‑101 (MQ=255)


                                                                                                cAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  1:1454864/1‑101 (MQ=255)


                                                                                                 aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGTGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   >  2:2311959/1‑101 (MQ=255)


                                                                                                  aGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  >  1:789907/1‑101 (MQ=255)


AGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.G.G.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640102‑2640297

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG