Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,208 Δ1 bp 18.2% intergenic (‑184/+92) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2080C→.18.2% 97.1 ‑1.5 ‑0.0165intergenic (‑184/+92)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/16):  ref base (79/55):  total (94/71)
Fisher's exact test strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.83e-01

Only 100 of 169 total aligned reads displayed.

AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCG  >  NC_014034/2640111‑2640308
                                                                                                   |                                                                                                    
aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                                   <  2:355009/101‑1 (MQ=21)
 aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                                  <  1:1652240/101‑1 (MQ=25)
  ccGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATggg                                                                                                 <  1:401648/101‑1 (MQ=25)
     tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                              <  2:553602/101‑1 (MQ=21)
     gggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                              <  2:2139839/100‑1 (MQ=25)
      ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                             <  2:2789973/101‑1 (MQ=25)
       gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                            <  2:898430/101‑1 (MQ=25)
        cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCATCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATc                                                                                           >  1:1989832/1‑101 (MQ=21)
          cTTGTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                                         <  1:2347939/101‑1 (MQ=25)
           ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                                        <  2:2517352/101‑1 (MQ=25)
              tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGcgt                                                                                     <  2:65143/101‑3 (MQ=21)
               gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTAGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                    <  1:4563587/101‑1 (MQ=25)
               gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                    <  1:2311517/101‑1 (MQ=21)
                cgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                                                   <  2:3073057/101‑1 (MQ=25)
                  gccccgaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                                <  2:2641040/95‑1 (MQ=14)
                   agccAAAAGGGGGCCGCTGCCCCCCTCTT.GGTCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                                <  2:746158/98‑1 (MQ=25)
                    ggCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                               <  2:3575984/101‑1 (MQ=21)
                    cccgaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                              <  2:2473130/97‑1 (MQ=17)
                      cgaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                            >  2:4119652/3‑101 (MQ=17)
                       gaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                           <  1:1215644/100‑1 (MQ=17)
                        aaaAGGGGGCCCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                          <  1:258985/101‑1 (MQ=18)
                         aaagggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                         >  2:3705510/4‑101 (MQ=17)
                           agggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                       >  1:1356112/2‑101 (MQ=18)
                            gggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                                       <  2:1352989/101‑1 (MQ=25)
                              gggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                    >  2:4392741/1‑99 (MQ=18)
                              gggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                    <  2:2966740/101‑3 (MQ=18)
                              gggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                                     >  2:2721403/1‑101 (MQ=25)
                               ggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCggggggga                                                                   >  2:2151925/1‑99 (MQ=18)
                               gccgcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                                   <  1:1084325/99‑4 (MQ=21)
                                gcgcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                                  <  1:4293630/101‑5 (MQ=21)
                                 cgcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                 <  2:240521/101‑6 (MQ=21)
                                 cgcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                 <  2:2002466/101‑6 (MQ=21)
                                  gcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                                >  1:3459872/1‑95 (MQ=21)
                                   ttGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                <  1:2032567/100‑1 (MQ=25)
                                   cTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                               >  1:2889601/1‑94 (MQ=25)
                                    tGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGCGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCCAATATCCCGGGGGGGGGCCt                                                               >  2:1749875/1‑101 (MQ=21)
                                    tGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcca                                                               >  2:2032427/1‑100 (MQ=25)
                                    tGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCCGGAGATTTGCGGCAAGGTGAAAGGA..TGGGGTATATCTTGCCCCCAATATCCCgagggtgcggcga                                                              >  2:317634/1‑93 (MQ=18)
                                     gCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggggaggcgaa                                                             >  1:127641/1‑93 (MQ=25)
                                      ccccccTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                                             >  2:1541300/1‑101 (MQ=25)
                                      ccccccTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                                             >  2:1899673/1‑101 (MQ=25)
                                         cccTCTT.GGCCTTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGTCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                          <  1:3644658/101‑1 (MQ=25)
                                         cccTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                          >  1:2889481/1‑101 (MQ=37)
                                          ccTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                         >  1:462203/1‑101 (MQ=37)
                                          ccTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                         <  1:1402267/101‑1 (MQ=37)
                                          ccTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                         <  1:3501361/101‑1 (MQ=37)
                                           ctctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                        >  1:3527331/1‑101 (MQ=37)
                                            tctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                       >  1:3813506/1‑101 (MQ=37)
                                            tctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                       <  1:1242583/101‑1 (MQ=37)
                                                     ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                               <  2:3205341/101‑1 (MQ=255)
                                                     ttCGGACAAGTCACCCCCCGAGGTATTTGGGGCAAGATGAAAA.ACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                               <  2:1841463/101‑1 (MQ=25)
                                                      tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                              >  2:1080611/1‑101 (MQ=37)
                                                        ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                            <  1:1063811/101‑1 (MQ=255)
                                                        ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                            >  1:1294296/1‑101 (MQ=37)
                                                         gCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                           <  1:3351117/101‑1 (MQ=255)
                                                         gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                           >  2:158525/1‑101 (MQ=37)
                                                           cAATTCACCCACCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                         >  2:2318962/1‑101 (MQ=25)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                       >  2:2746508/1‑101 (MQ=37)
                                                             aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                       >  1:4191721/1‑101 (MQ=255)
                                                              ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                      <  2:1371737/101‑1 (MQ=255)
                                                                 cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                   <  2:2067508/100‑1 (MQ=255)
                                                                  ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                  <  1:1749994/101‑1 (MQ=255)
                                                                  cccaccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                  <  1:2319124/101‑1 (MQ=255)
                                                                      ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                              >  1:1167436/1‑101 (MQ=255)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                            >  2:34989/1‑101 (MQ=255)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                            >  1:4412355/1‑101 (MQ=255)
                                                                        gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCt                            >  2:1794586/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                           >  1:2481085/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                           >  2:3117512/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                           >  2:289868/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                           <  1:2129859/101‑1 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                           >  1:4329800/1‑101 (MQ=255)
                                                                         aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                           >  1:1691652/1‑101 (MQ=255)
                                                                            tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                        >  1:3314646/1‑101 (MQ=255)
                                                                            tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                        >  2:3426415/1‑101 (MQ=255)
                                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGAACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                       >  1:1498210/1‑101 (MQ=255)
                                                                             aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCATGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTATTtatc                       >  1:860266/1‑101 (MQ=25)
                                                                              tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                      >  2:3242799/1‑101 (MQ=255)
                                                                                tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                    >  1:2135655/1‑101 (MQ=255)
                                                                                tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                    <  1:2606822/101‑1 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                   <  2:3813221/101‑1 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                   >  1:1476741/1‑101 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                   >  2:1075966/1‑101 (MQ=255)
                                                                                 ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                   >  2:2639042/1‑101 (MQ=255)
                                                                                  gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg                  >  1:3223233/4‑101 (MQ=255)
                                                                                   agcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt                 >  1:2821026/3‑101 (MQ=255)
                                                                                     cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc               >  2:3984726/1‑101 (MQ=255)
                                                                                      aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc              <  1:981903/101‑1 (MQ=255)
                                                                                       aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg             >  2:3689162/1‑101 (MQ=255)
                                                                                       aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg             >  1:789907/1‑101 (MQ=255)
                                                                                         gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg            >  2:4115085/5‑101 (MQ=255)
                                                                                         gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg            >  1:656106/5‑101 (MQ=255)
                                                                                          atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc           >  1:4487681/4‑101 (MQ=255)
                                                                                           tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt          >  1:3107867/3‑101 (MQ=255)
                                                                                            gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc         >  1:4622746/2‑101 (MQ=255)
                                                                                             aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa        >  2:4197405/1‑101 (MQ=255)
                                                                                              aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTCCGGCTCAg       >  1:4593618/1‑101 (MQ=255)
                                                                                                gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa     >  2:669812/1‑101 (MQ=255)
                                                                                                  ccATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAcc   >  2:4425626/1‑101 (MQ=255)
                                                                                                   cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg  >  1:4443634/1‑101 (MQ=255)
                                                                                                   |                                                                                                    
AACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCG  >  NC_014034/2640111‑2640308

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG