Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,770,912 Δ1 bp 3.2% intergenic (‑330/‑84) RCAP_rcc02582 ← / → lon hypothetical protein/ATP‑dependent protease La

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,770,9120C→. 3.2% 7.7 ‑2.6 ‑0.1158intergenic (‑330/‑84)RCAP_rcc02582/lonhypothetical protein/ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (96/57):  total (97/61)
Fisher's exact test strand distribution p-value = 7.35e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.13e-01

Only 100 of 212 total aligned reads displayed.

TCGAACCAAGCCGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTCCCCTC  >  NC_014034/2770812‑2771012
                                                                                                     |                                                                                                    
tCGAACCAAGCCGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.c                                                                                                      <  1:2998802/101‑1 (MQ=255)
       aaGCCGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.cccccccc                                                                                               >  2:2983604/1‑101 (MQ=255)
       aaGCCGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGGCGGGTCGGCGCCGTTGAACTGGGGCT.cccccccc                                                                                               >  1:798553/1‑101 (MQ=255)
          ccGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCTGTTCGGCGCGGTTGAACTGTGCCT.ccccccccccc                                                                                            >  2:1603401/1‑100 (MQ=255)
          ccGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCt                                                                                            <  1:1693042/101‑1 (MQ=255)
           cGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTGGGGTGGGCGCGGTTGAAATTGGCCT.cccccccccccc                                                                                           >  1:1965177/1‑99 (MQ=255)
           cGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTa                                                                                           <  1:13337/101‑1 (MQ=255)
           cGGGCGTGCGATGCGGGGCGTTCTGCCGCAGTGGGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTa                                                                                           <  2:1860898/101‑1 (MQ=255)
            gggGTTGGGGTGCCGGGCGGTCCGCCGGCGGGGGAAGGCTTGATGGCCGCGGGGCGGGCTGTCGGTTCGGGCCCGGTTAACCGTGCCT.CCCCCCCCCCTAg                                                                                          <  2:506686/101‑1 (MQ=255)
            gggCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGGGCAGTTGAACTGTGCCT.cccccccccccaa                                                                                          >  2:4317516/1‑98 (MQ=255)
             ggCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGGGCAGTTGAACTGTGCCT.CCCCCCCCCCCAGa                                                                                         >  1:3228095/1‑101 (MQ=255)
             ggCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCCAGa                                                                                         >  2:1704789/1‑101 (MQ=255)
               tttcgggtggggggGGTTCTGCCGCAGGGGTAAGGCTTGAGGACGGGGGGGGGGGCTTGCGGTTCGGCGCAGTTGAACTTGGCCC.CCCCCCCCCCTAGata                                                                                       <  2:84906/91‑1 (MQ=255)
               cTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGata                                                                                       <  2:3549512/101‑1 (MQ=255)
                ttGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGGTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCCAGatat                                                                                      >  1:3036198/1‑101 (MQ=255)
                ttGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGGGCAGTTGAACTGTGCCT.CCCCCCCCCCTCGatat                                                                                      >  1:3867682/1‑101 (MQ=255)
                ttGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGatat                                                                                      >  1:212723/1‑101 (MQ=255)
                ttGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGatat                                                                                      >  1:653142/1‑101 (MQ=255)
                     gttcGGGGGGTTCCGCCGGAGTTGGAAGACTTTATGACGGGGGGGCGGGCTGGCGGTTCGGCGCAGTTGAACTGTGCCC.CCCCCCCCCCTAGATATGGGGc                                                                                 <  2:3971858/98‑1 (MQ=255)
                     aTGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGGCGGTTCCGCGCAGTTGAACTTTGCCT.CCCCCCCCCCTAGATATGGGGc                                                                                 <  2:2201695/101‑1 (MQ=255)
                        gggggGGTTTTGCCGCGGTGGAAAGACTTGAGGCGGGGGGGGGGGGCTGTGGTTTCGGCGCGGTTAAACTGT.CCC.CCCCCCCCCCTAGATATGGGGCATgg                                                                             <  1:521754/100‑1 (MQ=255)
                         ggggCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGGGGGGTGTCGGGTCGGCGCAGGTGAACTGTGCCT.CCCCCCCCCCCCAATATggggggtgg                                                                             >  1:1991987/1‑96 (MQ=255)
                         ggggCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATgg                                                                             >  2:619592/1‑101 (MQ=255)
                          gggCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGGTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCCAGATATGGGGCATggg                                                                            >  2:2620022/1‑101 (MQ=255)
                          gggCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATggg                                                                            <  2:3164795/101‑1 (MQ=255)
                              gTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCCAGATTTGGGGGCTGGGAAgg                                                                        >  2:3175715/1‑101 (MQ=255)
                              gTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCCAGATATGGGGCATGGGAAgg                                                                        >  2:1157436/1‑101 (MQ=255)
                               ttCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAggg                                                                       >  1:1125118/1‑101 (MQ=255)
                                  tggcccAGTGTGGAGTCTTGATGTCGGGGGGGGGGGCTTTTGGTTGGGCGGCGTTGAACTGTGGCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACg                                                                    <  2:2213316/96‑1 (MQ=255)
                                   gCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCCCGATATGGGGCATGGGAAgggcggg                                                                   >  2:1366054/1‑97 (MQ=255)
                                      gCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGtt                                                                <  1:1002402/101‑1 (MQ=255)
                                          tgtgAAGACTTGATGACGGCGGGGCGGGCTGTTGGTTCGGCGCAATTGAACTGTGCCT.CCCCCCCCCCCAGGTATTGGGCATGGGAAGGGACGGGTTGCCa                                                            >  2:4568476/1‑101 (MQ=255)
                                          tgtgAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCTCCCCCCCCCCCTAGATATGGGGGATGGGAAGGGAGGGGGTGcc                                                             >  1:3186843/1‑101 (MQ=255)
                                          tgagAAGACTTGATGACAGCGGAGCGGGCCGTCGGTTCGGCGCAGTCGAACTGTGCCC.CACACCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCa                                                            <  2:798479/98‑1 (MQ=255)
                                           gtgAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCaa                                                           >  1:3373875/1‑101 (MQ=255)
                                            tgAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAAt                                                          >  2:1665773/1‑101 (MQ=255)
                                            ggAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT..CCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATc                                                         <  1:268050/100‑1 (MQ=255)
                                             gAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTGGAATTGGGGCATGGGAAGGGAGGGGGTGCCAATc                                                         >  1:4188695/1‑101 (MQ=255)
                                             gAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATc                                                         <  2:1557451/101‑1 (MQ=255)
                                             gAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATc                                                         >  1:4671487/1‑101 (MQ=255)
                                             gAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATc                                                         >  2:237766/1‑101 (MQ=255)
                                              aaGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATcg                                                        <  1:1508152/101‑1 (MQ=255)
                                               aGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGCCGGGTTGCCAATcgc                                                       <  1:3932318/101‑1 (MQ=255)
                                               aGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGGTGCCAATcgc                                                       >  1:1723845/1‑101 (MQ=255)
                                                gACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATcgcg                                                      <  1:2540622/101‑1 (MQ=255)
                                                    ttatggggggggggcgggCTGTCGGTTCGGGGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGAc                                                  <  2:4228598/91‑1 (MQ=255)
                                                     gaggccgggggGGGGGGCGGTCGGTTGGGCGCGTTTAACCTGTCCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGAcc                                                 <  1:3969701/96‑1 (MQ=255)
                                                       tgaCGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGCGGGGTTGCCAATCGCGGGACCAg                                               >  1:4125810/1‑101 (MQ=255)
                                                       tgaCGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAg                                               <  1:1631067/101‑1 (MQ=255)
                                                       tgaCGGAGGGGCGGGCTTGCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAg                                               <  1:2049371/101‑1 (MQ=255)
                                                         gcggtggTGCGGGCTGGCGGTTCGGGGCAGTTTGACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTg                                             <  2:1894915/100‑1 (MQ=255)
                                                         aCGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTg                                             <  2:4450456/101‑1 (MQ=255)
                                                              ggggcgggCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGGGGGACCAGTGCCttt                                        >  2:3073685/1‑101 (MQ=255)
                                                                ggcgggcTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTcg                                      <  2:2059536/101‑1 (MQ=255)
                                                                ggcgggcTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGAAATGGGGCATGGGGAGGGACGGGGTGCCAATCCCGGGGCCGGTGGCTTTcg                                      >  1:4321285/1‑101 (MQ=255)
                                                                ggcgggcTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCCTGGTATTGGGCGTGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGGCTTTcc                                      >  2:3877776/1‑100 (MQ=255)
                                                                  cgggcTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTcgcc                                    <  1:2214391/101‑1 (MQ=255)
                                                                   gggcTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGGGCCTTTcgccg                                   >  1:1511167/1‑101 (MQ=255)
                                                                     gcTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCa                                 >  2:2420023/1‑101 (MQ=255)
                                                                     gcTGTCGGTTCGGCGCAGTTGAACCGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCa                                 >  1:599318/1‑101 (MQ=255)
                                                                        ttCGGTTCGGCGCAGTTGAACTGTGCCT..CCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACa                             <  2:2437016/100‑1 (MQ=255)
                                                                          cGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACaa                            <  2:653080/101‑1 (MQ=255)
                                                                            gTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAAAAATGGGGGATGGGAAGGGGGGGGGGGCCAATCCCGGGGCCCGGGCCTTTCGCCCCAACCaaag                          >  1:873708/1‑99 (MQ=255)
                                                                             ttCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGga                         >  1:1480809/1‑101 (MQ=255)
                                                                             ttCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGga                         <  1:2234394/101‑1 (MQ=255)
                                                                               cGGCTCAGTTGAACTGTGCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGgaca                       <  2:932273/101‑1 (MQ=255)
                                                                               cGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTTCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGgaca                       >  1:4628102/1‑101 (MQ=255)
                                                                               cGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGgaca                       <  2:4652140/101‑1 (MQ=255)
                                                                               cGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGgaca                       >  2:30965/1‑101 (MQ=255)
                                                                                 gcgcAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGgacaga                     <  1:4524446/101‑1 (MQ=255)
                                                                                  cgcAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGCCCAGTGCCTTTCGCCCCAAACAAGgcaagac                    >  1:1889914/1‑101 (MQ=255)
                                                                                   gcAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGgacagaca                   >  1:2368628/1‑101 (MQ=255)
                                                                                   gcAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGgacagaca                   >  1:2346886/1‑101 (MQ=255)
                                                                                     aGTTTAACTGTGCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAAt                 <  1:382654/101‑1 (MQ=255)
                                                                                      gTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGGGCCTTTCGCCGCAAACAAGGACAGACAATg                >  1:1177340/1‑101 (MQ=255)
                                                                                      gTTGAACTGTGCCT.CCCCCCCCCCTAAATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGAACCAGTGCCTTTCCCCGCAAACAAGGAAAGAAAATg                >  1:1405480/1‑101 (MQ=255)
                                                                                       ttggattgtgCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGa               >  1:3759750/7‑101 (MQ=255)
                                                                                       ttGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGa               <  2:4034734/101‑1 (MQ=255)
                                                                                       gtGAACGGGGCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGa               <  1:3132476/100‑1 (MQ=255)
                                                                                         taaCTGTGCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGAcc             <  2:3029319/100‑1 (MQ=255)
                                                                                         gAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGAcc             <  2:3142689/101‑1 (MQ=255)
                                                                                         gAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGAcc             >  2:681781/1‑101 (MQ=255)
                                                                                          aaCTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCg            <  2:1226870/101‑1 (MQ=255)
                                                                                          aaCTGTGCCT.CCCCCCCCCCTAGATACGGGGCATGGGAAGGGACGCGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCg            <  1:3920651/101‑1 (MQ=255)
                                                                                           aactggtgccccccccctccTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCg            <  2:4188407/91‑1 (MQ=255)
                                                                                           aCTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGc           >  2:2265672/1‑101 (MQ=255)
                                                                                            cTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCa          <  2:2134817/101‑1 (MQ=255)
                                                                                            cTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCa          >  2:1945968/1‑101 (MQ=255)
                                                                                            cTGTGCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCa          <  2:1305940/101‑1 (MQ=255)
                                                                                            cTGTGCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCa          <  1:2104961/101‑1 (MQ=255)
                                                                                             tgtgCCT..CCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACt        >  2:3469988/1‑101 (MQ=255)
                                                                                              gtgCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGGGCCTTTCGCCGCAAAAAAGGACAGAAAATGCCCGcccc        >  2:3715180/1‑98 (MQ=255)
                                                                                               tgCCC.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTc       <  1:2620207/101‑1 (MQ=255)
                                                                                                gCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTcc      >  2:3505072/1‑101 (MQ=255)
                                                                                                  cT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTcccc    >  1:4232180/1‑101 (MQ=255)
                                                                                                   t.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTCCCCt   >  1:1353700/1‑101 (MQ=255)
                                                                                                    cccccccccccTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTCCCCt   <  2:1543845/100‑1 (MQ=255)
                                                                                                    cccccccccccTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTCCCCt   <  1:4421478/100‑1 (MQ=255)
                                                                                                    cccccccccccTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTCCCCt   <  2:289499/100‑1 (MQ=255)
                                                                                                     ccccccccccTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTCCCCTc  <  2:2561348/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
TCGAACCAAGCCGGGCTTGCGATGCGGGGCGTTCTGCCGCAGTGTGAAGACTTGATGACGGCGGGGCGGGCTGTCGGTTCGGCGCAGTTGAACTGTGCCT.CCCCCCCCCCTAGATATGGGGCATGGGAAGGGACGGGTTGCCAATCGCGGGACCAGTGCCTTTCGCCGCAAACAAGGACAGACAATGACCGCACTCCCCTC  >  NC_014034/2770812‑2771012

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 41 ≤  ATCG