Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,708 Δ1 bp 3.1% intergenic (+51/‑109) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7080G→. 3.1% 13.2 ‑2.1 ‑1.1229intergenic (+51/‑109)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (114/108):  total (120/109)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.27e-02

Only 100 of 247 total aligned reads displayed.

AGCTGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAC  >  NC_014034/2950608‑2950808
                                                                                                    |                                                                                                     
aGCTGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATg                                                                                                       >  2:3116939/1‑101 (MQ=255)
aGCTGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATg                                                                                                       <  2:2753328/101‑1 (MQ=255)
aGCTGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATg                                                                                                       >  2:1260214/1‑101 (MQ=255)
 gCTGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGa                                                                                                      <  2:3323242/101‑1 (MQ=255)
 gCTGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGa                                                                                                      <  1:2101061/101‑1 (MQ=255)
  cTGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTCGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGaa                                                                                                     <  2:3601434/101‑1 (MQ=255)
    gCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAac                                                                                                   <  2:2990447/101‑1 (MQ=255)
     cccGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCACCGGGATATTTGAGGAAAGATGAAaca                                                                                                  >  2:2263409/1‑101 (MQ=255)
      ccGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAacac                                                                                                 >  1:1738059/1‑101 (MQ=255)
       cGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACg                                                                                                >  2:3805686/1‑101 (MQ=255)
           gTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGcccc                                                                                            >  1:3086213/1‑101 (MQ=255)
           gTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGcccc                                                                                            <  1:1787118/101‑1 (MQ=255)
             gccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCtt                                                                                          <  2:2607033/101‑1 (MQ=255)
              ccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCttt                                                                                         <  1:1445082/101‑1 (MQ=255)
               cccccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTc                                                                                        <  1:480125/99‑1 (MQ=255)
                gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                       >  1:1975510/1‑101 (MQ=255)
                gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                       <  1:672649/101‑1 (MQ=255)
                gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                       <  2:3784391/101‑1 (MQ=255)
                 cccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCAt                                                                                      <  1:3932125/101‑1 (MQ=255)
                 cccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCAt                                                                                      <  1:108704/101‑1 (MQ=255)
                    tCGCCGCAATATGCGGACCTTAATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGCCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCtt                                                                                   <  1:1124427/101‑1 (MQ=255)
                      gccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGAGATATTTGAGGCAAGATGAAACACGCCCCTTTCGTCTAGc                                                                                 >  1:749400/1‑101 (MQ=255)
                        cgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGccc                                                                               >  1:4197477/1‑101 (MQ=255)
                           aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                            >  2:4413940/1‑101 (MQ=255)
                             tatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAat                                                                          >  2:3443760/1‑101 (MQ=255)
                              atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                         >  1:2328251/1‑101 (MQ=255)
                                gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                       >  2:2174292/1‑101 (MQ=255)
                                gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                       >  2:4015178/1‑101 (MQ=255)
                                gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                       >  1:1826386/1‑101 (MQ=255)
                                  ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTTGAGGGAAGATGAAAGACGCCCCTTTCATTTTTCCCCAAATATccc                                                                     >  1:852845/1‑101 (MQ=25)
                                  ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                     >  2:1716949/1‑101 (MQ=255)
                                  ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                     <  2:1169979/101‑1 (MQ=255)
                                  ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                     <  1:3805971/101‑1 (MQ=255)
                                   gAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.g                                                                   >  2:1418998/1‑101 (MQ=255)
                                    aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gg                                                                  <  2:4205115/101‑1 (MQ=255)
                                     aCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggg                                                                 <  1:1260307/101‑1 (MQ=255)
                                       ttGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggggg                                                               >  2:154569/1‑101 (MQ=255)
                                         gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                             <  1:3767988/101‑1 (MQ=255)
                                            ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAt                                                          <  1:1710253/101‑1 (MQ=255)
                                             tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAAtt                                                         >  2:1872361/1‑101 (MQ=255)
                                             tCTGAAGCAAGCCGGGGCCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAtt                                                         <  2:2354138/101‑1 (MQ=255)
                                               tGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTg                                                       <  1:3345757/101‑1 (MQ=255)
                                               tGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTg                                                       >  2:2932716/1‑101 (MQ=255)
                                                gAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGc                                                      >  1:2268050/1‑101 (MQ=255)
                                                 aagcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGcc                                                     >  1:2608436/1‑101 (MQ=255)
                                                  agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                    <  2:640020/101‑1 (MQ=255)
                                                    caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                  >  2:2785142/1‑101 (MQ=255)
                                                    caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                  <  2:4051662/101‑1 (MQ=255)
                                                     aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                 >  2:1563630/1‑101 (MQ=255)
                                                     aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTAATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                 >  1:4418369/1‑101 (MQ=255)
                                                      agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGTCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                >  1:3697286/1‑101 (MQ=255)
                                                       gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAAGACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                               >  1:4562086/1‑101 (MQ=255)
                                                       gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                               <  2:719133/101‑1 (MQ=255)
                                                         cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                             <  1:1417590/101‑1 (MQ=255)
                                                         cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                             <  2:2448282/101‑1 (MQ=255)
                                                         cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                             >  2:556662/1‑101 (MQ=255)
                                                          gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                            >  2:510913/1‑101 (MQ=255)
                                                           ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaaa                                           <  1:29375/101‑1 (MQ=255)
                                                             ggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAaga                                         <  2:2526065/101‑1 (MQ=255)
                                                               ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgg                                       <  2:2154258/101‑1 (MQ=255)
                                                                tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggg                                      <  2:3085969/101‑1 (MQ=255)
                                                                 ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGGATTTGCCGTCAGGCCAAGAgggg                                     >  1:3413912/1‑101 (MQ=255)
                                                                 ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                     <  2:327772/101‑1 (MQ=255)
                                                                 ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                     >  2:4284328/1‑101 (MQ=255)
                                                                  ttcccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggggg                                    <  2:825051/99‑1 (MQ=255)
                                                                   ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggc                                  >  1:1373142/9‑99 (MQ=25)
                                                                   gcttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGGGAATTGGCC.AAAGGCCAAGAGGGGGCa                                  >  1:4449982/10‑101 (MQ=2)
                                                                   gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGc                                   <  2:3573432/101‑1 (MQ=255)
                                                                    tcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggca                                 <  2:3005747/94‑4 (MQ=25)
                                                                    cttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAg                                 <  1:280985/93‑1 (MQ=2)
                                                                    cttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAg                                 <  1:2544062/93‑1 (MQ=2)
                                                                     cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggcag                                >  1:363569/7‑97 (MQ=25)
                                                                     cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggcag                                >  2:2659336/7‑97 (MQ=25)
                                                                     ccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAg                                 >  1:2469077/1‑101 (MQ=255)
                                                                     ccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAg                                 <  1:1992223/101‑1 (MQ=255)
                                                                     ccccGCACCCCCGGGATATGTGAGGCAAGATGAATCTCGCCACTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAg                                 <  2:870315/101‑1 (MQ=25)
                                                                        cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTc                              >  1:3588810/1‑101 (MQ=255)
                                                                         ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcc                             >  1:567581/1‑101 (MQ=255)
                                                                          gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccc                            <  1:3733935/101‑1 (MQ=255)
                                                                          gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccc                            <  1:707198/101‑1 (MQ=255)
                                                                           aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcccc                           >  2:2007493/1‑101 (MQ=255)
                                                                             ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                         <  1:2217595/101‑1 (MQ=255)
                                                                                cGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcc                      <  2:1003602/101‑1 (MQ=255)
                                                                                  ggATATTTGAGGCAACATGAACCTCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGCCACCTCCCGCTGccgc                    <  2:3697011/101‑3 (MQ=25)
                                                                                   gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                   >  2:1379935/1‑101 (MQ=255)
                                                                                    atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCgg                  >  1:2445185/1‑101 (MQ=255)
                                                                                     tatTTGAGGCAAGATGAAACCCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                 <  1:4293749/101‑1 (MQ=255)
                                                                                     tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                 >  1:3142713/1‑101 (MQ=255)
                                                                                      atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                <  1:1571555/101‑1 (MQ=255)
                                                                                      atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                >  2:2424968/1‑101 (MQ=255)
                                                                                          gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg            >  1:985354/1‑101 (MQ=255)
                                                                                          gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg            >  2:442864/1‑101 (MQ=255)
                                                                                           aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg           >  2:2194679/1‑101 (MQ=255)
                                                                                              cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg        >  2:3537657/1‑101 (MQ=255)
                                                                                              cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg        >  1:3001757/1‑101 (MQ=255)
                                                                                              cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg        >  1:2114588/1‑101 (MQ=255)
                                                                                                aGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCg      >  1:2776931/1‑101 (MQ=255)
                                                                                                  aTGAAACACGCCCCTTTCATCTTGCCCGAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGa    >  2:3225652/1‑101 (MQ=255)
                                                                                                   tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa   <  1:4543567/101‑1 (MQ=255)
                                                                                                   tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa   >  2:1043623/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
AGCTGCCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAC  >  NC_014034/2950608‑2950808

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG