Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,995,678 Δ1 bp 3.5% intergenic (+218/+2) RCAP_rcc02802 → / ← RCAP_rcc02803 membrane dipeptidase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,995,6780G→. 3.5% 2.5 0.0 ‑0.285intergenic (+218/+2)RCAP_rcc02802/RCAP_rcc02803membrane dipeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (64/18):  total (67/18)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.59e-01

GGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTCGAA  >  NC_014034/2995578‑2995778
                                                                                                         |                                                                                                    
ggcggcCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGGGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCg                                                                                                      >  1:4607369/1‑101 (MQ=255)
   ggcCCGGCCCCCTGCTTTCCTGTGC.AAAAATCGG.CGGGGGGGTGCGGGGGGGGGGCCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTc                                                                                                   <  2:4436225/101‑1 (MQ=16)
     cccAGCGCCCT.CTTTTCCTTGGACAAAATGCG.CGGGGGGGTGGGGGGGGGGGGCCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAg                                                                                                 <  2:403594/101‑1 (MQ=25)
      ccAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGt                                                                                                >  2:1417389/1‑101 (MQ=255)
         cggctttgtttttCCTTGG.AAAAATACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCAGCCGAGGCGAAATACCCC.CTCAgc                                                                                                >  2:2260547/10‑100 (MQ=0)
           gcCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGGCGCCCCGGCTT..GCCCGGGCGAAATCCCCCGCCCAATCCCag                                                                                           >  2:1915329/1‑101 (MQ=18)
                gtttttCCTTGG.AAAAATACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCAGCCGAGGCGAAATACCCC.CTCAGCccttcat                                                                                         >  1:1058553/3‑96 (MQ=0)
                  ttttCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACttt                                                                                    >  1:2991227/1‑101 (MQ=255)
                   tttCCTGGG.CAAGAGACG.GCGGGGGGGGGGGGGGGGGCGGGCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTc                                                                                   <  1:1362820/101‑1 (MQ=25)
                    ttCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGGCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCCGTCCCCCAGACTTTCg                                                                                  >  1:2053905/1‑101 (MQ=21)
                     tCCTTGG.AAAAATACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCAGCCGAGGCGAAATACCCC.CTCAGCccttcatcttca                                                                                    >  2:3659601/1‑91 (MQ=0)
                        ctggaaaaaaTCCG.GGGGGGGGGGGGGGGGGCGGTCCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGAcc                                                                               <  1:3123620/95‑1 (MQ=25)
                          gg.CAAAATACG.CCGGGGGGTGCGGGGGGCAGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCg                                                                            >  1:4528715/1‑101 (MQ=255)
                                aaTACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGATCAGTCCCCGAGACTTTCACCCTCGAACCg                                                                       >  2:1088520/1‑101 (MQ=25)
                                  tACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGCCGCCCCGGCCT..GGCGGGGCGAAAACCCCCGCTCAGTCCCCGAGACTTTCGACCTCGAACCggg                                                                     >  1:3081407/1‑101 (MQ=37)
                                           ggggTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGAcgc                                                             >  1:596885/1‑101 (MQ=255)
                                            gggTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGAcgcg                                                            >  1:1300790/1‑101 (MQ=255)
                                              gTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGCCGCCGa                                                          >  2:952220/1‑101 (MQ=255)
                                                gCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATg                                                        <  1:199170/101‑1 (MQ=255)
                                                gCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATg                                                        >  1:1771106/1‑101 (MQ=255)
                                                gCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCACAGAATTTCGACCTCGAACCGGGGCAGACGCGGATg                                                        >  2:602670/1‑101 (MQ=255)
                                                 cGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAATCCCAGAGACTTTCGACCTCGCACCCGGCCCGACGCGGATgc                                                       >  2:2518057/1‑101 (MQ=38)
                                                  ggggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATgcg                                                      >  2:3420874/1‑101 (MQ=255)
                                                  ggggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATgcg                                                      >  2:3504661/1‑101 (MQ=255)
                                                  ggggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATgcg                                                      >  1:3097920/1‑101 (MQ=255)
                                                  ggggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGGCAGACGCGGATgcg                                                      >  1:1270322/1‑101 (MQ=255)
                                                   gggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATgcgc                                                     >  2:3386575/1‑101 (MQ=255)
                                                   gggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGGCAGACGCGGATgcgc                                                     >  2:392222/1‑101 (MQ=255)
                                                      ggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAgg                                                  >  1:2403843/1‑101 (MQ=255)
                                                      ggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAgg                                                  <  1:333612/101‑1 (MQ=255)
                                                       gCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGc                                                 >  2:3670598/1‑101 (MQ=255)
                                                        cGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCg                                                >  1:3492674/1‑101 (MQ=255)
                                                         ggccccccccG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCgg                                               <  1:2518231/97‑1 (MQ=255)
                                                         ggAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCgg                                               >  2:3372982/1‑101 (MQ=255)
                                                         ggAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCgg                                               >  1:2275589/1‑101 (MQ=255)
                                                          gAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCggg                                              >  1:143844/1‑101 (MQ=255)
                                                          gAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCggg                                              >  2:1258319/1‑101 (MQ=255)
                                                          gAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCggg                                              >  2:3109753/1‑101 (MQ=255)
                                                           aGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGc                                             >  2:4376988/1‑101 (MQ=255)
                                                           aGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGc                                             >  2:3306607/1‑101 (MQ=255)
                                                            gCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGTGcc                                            >  1:722063/1‑101 (MQ=255)
                                                            gCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCCCAGTCCCAGAGACTTTCGACCTCGAACCGGGGCAGACGCGGATGGGCAGGCGGGcc                                            >  1:884355/1‑101 (MQ=255)
                                                               ccccG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCATAGACTTTCGACCTCGAACCGGGCCAGACGCGGATGCGCAGGCGGGCCGaa                                         >  2:4589351/1‑101 (MQ=255)
                                                                ccgg.cgGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGaaa                                        <  2:2403038/98‑1 (MQ=255)
                                                                cccG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGaaa                                        >  2:1984235/1‑101 (MQ=255)
                                                                 ccG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAg                                       >  2:3390873/1‑101 (MQ=255)
                                                                  cg.cgGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGc                                      >  1:1944243/1‑101 (MQ=255)
                                                                  cg.cgGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGc                                      >  1:3197355/1‑101 (MQ=255)
                                                                     cgGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCg                                    >  2:4258746/1‑101 (MQ=255)
                                                                      gggTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGc                                   >  2:3815812/1‑101 (MQ=255)
                                                                      gggTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCCCAAGCCGc                                   >  2:3278915/1‑101 (MQ=255)
                                                                       ggTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCa                                  <  2:3668349/101‑1 (MQ=255)
                                                                       ggTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCa                                  >  1:1369421/1‑101 (MQ=255)
                                                                        gTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCaa                                 >  1:4260701/1‑101 (MQ=255)
                                                                         tCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAg                                <  1:2815667/101‑1 (MQ=255)
                                                                          cGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAgg                               >  2:4661810/1‑101 (MQ=255)
                                                                           gcaccGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGc                              >  1:2656614/4‑101 (MQ=255)
                                                                            ccccGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCt                             >  2:3635483/1‑101 (MQ=255)
                                                                            ccccGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCt                             >  1:3160357/1‑101 (MQ=255)
                                                                             cccGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTg                            >  2:4304256/1‑101 (MQ=255)
                                                                              ccGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCGgg                           >  2:3091148/1‑101 (MQ=255)
                                                                                ggCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCg                         >  1:3936322/1‑101 (MQ=255)
                                                                                 gCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGa                        >  2:3649390/1‑101 (MQ=255)
                                                                                  cTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGaa                       >  1:4202829/1‑101 (MQ=255)
                                                                                   tt..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAAt                      <  1:4259034/101‑1 (MQ=255)
                                                                                   tt..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAAt                      >  1:1500237/1‑101 (MQ=255)
                                                                                   tt..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAAt                      <  1:1088609/101‑1 (MQ=255)
                                                                                    t..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAAtt                     <  2:2990998/101‑1 (MQ=255)
                                                                                         cGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCg                  >  2:4256229/1‑101 (MQ=255)
                                                                                          ggggCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCgg                 >  1:509541/1‑101 (MQ=255)
                                                                                          ggggCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCgg                 <  1:1859652/101‑1 (MQ=255)
                                                                                          ggggCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCgg                 >  1:1149167/1‑101 (MQ=255)
                                                                                            ggCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGa               <  2:1393420/101‑1 (MQ=255)
                                                                                              cGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAAt             >  2:2267519/1‑101 (MQ=255)
                                                                                              cGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCAGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAAt             >  2:2381422/1‑101 (MQ=255)
                                                                                               gAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCTAATTGCGGGAAtt            >  1:2302351/1‑101 (MQ=255)
                                                                                               gAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAAtt            <  2:2053765/101‑1 (MQ=255)
                                                                                               gAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAAtt            >  2:2558240/1‑101 (MQ=255)
                                                                                               gAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAAtt            >  2:3559373/1‑101 (MQ=255)
                                                                                                aaaTCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTg           >  1:822891/1‑101 (MQ=255)
                                                                                                aaaTCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTg           >  2:3411495/1‑101 (MQ=255)
                                                                                                aaaTCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTCGCGAATTGCGGGAATTg           >  2:3928219/1‑101 (MQ=255)
                                                                                                 aaTCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTgc          >  2:1077817/1‑101 (MQ=255)
                                                                                                 aaTCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTgc          >  1:1292005/1‑101 (MQ=255)
                                                                                                   tCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTgcgc        >  2:3848891/1‑101 (MQ=255)
                                                                                                   tCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTgcgc        >  2:246170/1‑101 (MQ=255)
                                                                                                   tCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTgcgc        <  2:1270229/101‑1 (MQ=255)
                                                                                                     ccccGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCt      >  2:3539268/1‑101 (MQ=255)
                                                                                                     ccccGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCCGGCGAATTGCGGGAATTGCGCCt      >  2:1861890/1‑101 (MQ=255)
                                                                                                      cccGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTc     >  1:4085268/1‑101 (MQ=255)
                                                                                                      cccGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTc     >  1:15507/1‑101 (MQ=255)
                                                                                                        cGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTCGa   >  1:3687028/1‑101 (MQ=255)
                                                                                                        cGCTCACTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCCCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTCgt   <  1:4589650/101‑2 (MQ=255)
                                                                                                         gCTCAGTCCCAGAGATTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTCGaa  <  2:2605827/101‑1 (MQ=255)
                                                                                                         gCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTCGaa  <  1:4304545/101‑1 (MQ=255)
                                                                                                         gCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTCGaa  >  1:1547859/1‑101 (MQ=255)
                                                                                                         gCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTCGaa  >  1:2133913/1‑101 (MQ=255)
                                                                                                         |                                                                                                    
GGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGCCGCAAGGCTGGCGAATTGCGGGAATTGCGCCTCGAA  >  NC_014034/2995578‑2995778

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG