Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,943 Δ1 bp 5.0% coding (161/1467 nt) RCAP_rcc03392 → hemolysin D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,9430C→. 5.0% 12.0 0.0 ‑0.4121coding (161/1467 nt)RCAP_rcc03392hemolysin D
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (64/51):  total (67/54)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.14e-01

Only 100 of 156 total aligned reads displayed.

GCAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCGG  >  NC_014034/3596843‑3597041
                                                                                                    |                                                                                                   
gCAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGCGCGAACCGGAAGc                                                                                                     >  2:538635/1‑101 (MQ=255)
gCAACCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGc                                                                                                     <  1:3578827/101‑1 (MQ=255)
gCAACCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGc                                                                                                     <  1:302376/101‑1 (MQ=255)
  aacccccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGccc                                                                                                   <  2:1956158/98‑1 (MQ=255)
   acccccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGcccc                                                                                                  <  1:1930154/99‑1 (MQ=255)
    cccccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGccccc                                                                                                 <  2:1777976/100‑1 (MQ=255)
     ccccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCCGAAGcccccc                                                                                                >  2:2189664/1‑101 (MQ=255)
       ccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGcccccccc                                                                                              <  1:1765972/101‑1 (MQ=255)
          gCCGCAAGCCCCCCGCCCCCCTCGGCCCGCCCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCg                                                                                           <  1:4581707/101‑1 (MQ=255)
          gCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCg                                                                                           <  1:3886421/101‑1 (MQ=255)
          gCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCg                                                                                           <  1:3654685/101‑1 (MQ=255)
             ccAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGcc                                                                                        <  2:1025473/100‑1 (MQ=255)
             ccAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCACCCCCCGGcc                                                                                        <  1:395437/100‑1 (MQ=255)
              cAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCg                                                                                       >  2:154910/1‑101 (MQ=255)
               aaGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCgg                                                                                      >  1:3059727/1‑100 (MQ=255)
                aGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAAACGGAAGCCCCCCCCCCGGCCGAg                                                                                     >  1:1648961/1‑101 (MQ=255)
                 gcccccccccccccTCGGCCCGCCCGCGTTGCAGGCGGCAGGACCCGGCCTTCCCCCCCCGGCCGCCCGGGGGGACCCGGAACCCCCCCCCCCCGcccgga                                                                                    >  2:4090117/1‑97 (MQ=255)
                  ccccccgccccccCCGGCCCGACCGCGTTGCAGGCGGCAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGgc                                                                                   <  1:3323737/101‑1 (MQ=255)
                  cccccccccccccCCGGCCCGACCGCGGTGCCGGCGGCAGGGACCGGCCTTCCCCCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGgc                                                                                   <  1:2449563/101‑1 (MQ=255)
                   cccccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCGGCCGAGgcgc                                                                                 >  1:2987777/1‑101 (MQ=255)
                    ccccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGGGgcgc                                                                                 >  1:929876/1‑101 (MQ=255)
                    ccccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGgcgc                                                                                 <  1:4523711/101‑1 (MQ=255)
                       cgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCCCGGa                                                                              >  2:1396183/1‑101 (MQ=255)
                       ccccccccTCGGCCCGCCCCGGTGGCGGCCGCCGGGGCCCGCCCTCCCCCCCCCGGCCCGCCGGGGGGACCCGGAACCCCCCCCCCCGGCCGAGGCGCGGa                                                                              <  2:550086/99‑1 (MQ=255)
                        cccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGAt                                                                             <  1:366058/100‑1 (MQ=255)
                        cccccccCCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGAt                                                                             <  1:1133627/100‑1 (MQ=255)
                          cccccTCGGCCCGACCGCGTTGCAGGCGGCCGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGCCCCGCAtcc                                                                           >  1:557446/1‑100 (MQ=255)
                          cccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGAtct                                                                           <  1:4666824/101‑1 (MQ=255)
                          cccccCCGGCCCGACCGCGTTGCAGGCGGCAGGGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGAtct                                                                           <  1:148930/101‑1 (MQ=255)
                          cccccCCGGCCCGACCGCGTTGCAGGCGGCAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGAtct                                                                           <  1:3936118/101‑1 (MQ=255)
                          cccccCCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCGGCCGAGGCGCGGAtctc                                                                          <  1:4301023/101‑1 (MQ=255)
                            cccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCGGCCGAGGCGCGGATCTCGc                                                                        <  2:2633808/101‑1 (MQ=255)
                               ccGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGccc                                                                      <  2:4447833/100‑1 (MQ=255)
                                cGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCCGCCCGGGCGCGGCGCTCGcccc                                                                     >  2:82/1‑101 (MQ=255)
                                 ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCg                                                                    <  2:3933179/101‑1 (MQ=255)
                                 ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCg                                                                    <  2:162820/101‑1 (MQ=255)
                                        ccGCGTTGCAGGCGGCAGCGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGgcg                                                             <  2:4549135/101‑1 (MQ=255)
                                        ccGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCGGGGGCGCGGGGCTGGCCCCGGGGGgcg                                                             >  1:3407130/1‑101 (MQ=255)
                                        ccGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGgcg                                                             <  1:538683/101‑1 (MQ=255)
                                         cgcgTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGGGGCGCGGATCCCGCCCCGGGGGgcgc                                                            >  1:270103/1‑101 (MQ=255)
                                          gcgTTGCAGGCGGCAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgct                                                           <  1:2738940/101‑1 (MQ=255)
                                           cgTGGCGGGGGGAAGAGCCCGCCCTCCCCGCCCCGGCCCGCCGGAGCGAACCGGAA.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctgc                                                         <  2:1390782/101‑1 (MQ=255)
                                             ttGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctgct                                                        <  2:1921412/101‑1 (MQ=255)
                                                 aGGCGGCAGAGACCGGCCTTTCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATc                                                    >  2:313576/1‑101 (MQ=255)
                                                 aGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGGTGATc                                                    >  2:664468/1‑101 (MQ=255)
                                                      gcAGAGCCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.Ta                                              <  2:2002601/101‑1 (MQ=255)
                                                      gcAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.Ta                                              <  1:2279825/101‑1 (MQ=255)
                                                      gcAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCGGCCGAGGCGCGGAGCTCGCCCCCGGGGGCGCCGCTGGTCGGG.TAt                                             >  2:1444240/1‑101 (MQ=255)
                                                        agagACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGGCGGGGGGCGGTTCTCGCCCCGGGGGGCGCCGCGGATCGGccgtg                                             >  2:2957418/1‑97 (MQ=255)
                                                        agagACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGGTCTCGCCCCGGGGGGCGCCGCTGATCGGC.TATg                                            >  1:3289599/1‑101 (MQ=255)
                                                        agagACCGGCCTTCCCGCCCCGGCCCACCGGAGCAAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATg                                            <  2:3005761/101‑1 (MQ=255)
                                                          agaCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATgcc                                          >  2:3404325/1‑99 (MQ=255)
                                                          agaCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTc                                          >  2:1049158/1‑101 (MQ=255)
                                                          agaCCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTc                                          >  1:262907/1‑101 (MQ=255)
                                                             ccGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACg                                       <  1:4650077/101‑1 (MQ=255)
                                                              cGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCCCCCCGGGGGGCGCGGCTGATCGGC.TATGTCACGc                                      >  1:684176/1‑101 (MQ=255)
                                                              cGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCCCGGCGCGGATCTCTCCCCGGGTGGCGCTCCGGTTCGGC.TTTGTCAcct                                      >  2:4666134/1‑99 (MQ=255)
                                                               ggCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGGTCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCt                                     >  1:4655988/1‑101 (MQ=255)
                                                                 ccTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCACCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTg                                   >  2:1894971/1‑101 (MQ=255)
                                                                 ccTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAG.CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGc                                  <  1:2151363/101‑1 (MQ=255)
                                                                 ccTCCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTg                                   <  1:942368/101‑1 (MQ=255)
                                                                     cccGCCCGGGCCCGCCGGGGCGAACCGGGAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGt                               <  1:2189814/101‑1 (MQ=255)
                                                                     cccGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGt                               <  2:537295/101‑1 (MQ=255)
                                                                     cccGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCCCCCCGGGTGGCGCTGCTGATCGGC.TATGTCCCGCTTGCGGt                               >  2:395499/1‑101 (MQ=255)
                                                                      ccGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGtt                              <  1:154924/101‑1 (MQ=255)
                                                                       cGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGGGCTGCTGATCGGC.TATGTCACGCTTGCGGTTc                             >  1:762920/1‑101 (MQ=255)
                                                                        cccccGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCt                            <  1:2821996/100‑1 (MQ=255)
                                                                          cccGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTgg                          <  1:3415461/101‑1 (MQ=255)
                                                                          cccGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCCCGCTTGCGGTTCTgg                          >  1:2956216/1‑101 (MQ=255)
                                                                           ccGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGt                         <  1:3372563/101‑1 (MQ=255)
                                                                           ccGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTTTGGt                         >  1:2996938/1‑101 (MQ=255)
                                                                            cGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGGGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCCCGCTTGCGGTTCTGGTc                        >  1:910878/1‑101 (MQ=255)
                                                                             ggCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCg                       <  1:389010/101‑1 (MQ=255)
                                                                               cccGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCggg                     >  2:3753933/1‑101 (MQ=255)
                                                                                   ccgGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCCGGGGGCGCTGCTGATCGGC.TTTGTCACCCTTGCGGGTCTGGTCGGGGGCt                 >  2:2695722/1‑101 (MQ=255)
                                                                                    cgGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTTCGGTTCTGGTCGGGGGCtt                >  1:1574127/1‑101 (MQ=255)
                                                                                     ggAGCGAACCGGAAGCCCCCCCCCCGGCCGGGGCGCGGATCCCGCCCCGGGGGGGGCCGCTGGTCGGC.TTTGTCACGCTTGCGGTTCTGGTCGGGGGGTTc               >  1:1706520/1‑101 (MQ=255)
                                                                                     ggAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATAGGC.TATGTCCCGCTTGCGGTTCTGGTCGGGGGCTTc               >  2:172855/1‑101 (MQ=255)
                                                                                     ggAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGGGCTGGTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTc               >  1:1234506/1‑101 (MQ=255)
                                                                                       ggCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCgg             <  2:4655687/100‑1 (MQ=255)
                                                                                       ggCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCgg             <  1:2731689/100‑1 (MQ=255)
                                                                                       aGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCgg             >  2:2184632/1‑101 (MQ=255)
                                                                                        gCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCggg            <  2:205021/101‑1 (MQ=255)
                                                                                         cGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGc           <  2:3653412/101‑1 (MQ=255)
                                                                                          gAACCGGAAGCCCCCCCCCCGGCCGGGGGGCGGGTCTCCCCCCCGGGGGGGGCGCCGATCGGC.TTTTTTCCCCCTGCGGCTCTGGGCGGGGGGTTCggggt          >  1:958277/1‑99 (MQ=255)
                                                                                          gAACCGGAAG.CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCtt         >  2:2120900/1‑101 (MQ=255)
                                                                                           aaCCGGAAGCCCCCCCCCCGGCCGGGGGGGGGATTCAGCCCCGGGGGGGCCTG.TGGTCCGCTTATGTCACGCTGCGGGTTCTGGCCGGGGGCTTCGGGCtt         >  2:2676484/1‑101 (MQ=255)
                                                                                           aaCCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCtt         >  1:4494491/1‑101 (MQ=255)
                                                                                           aaCCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCtt         >  1:2949279/1‑101 (MQ=255)
                                                                                           aaCCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGCCGGGGGCTTGGGGCtt         >  2:2425532/1‑101 (MQ=255)
                                                                                            aCCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCttt        <  2:1740763/101‑1 (MQ=255)
                                                                                            aCCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCttt        <  2:2827023/101‑1 (MQ=255)
                                                                                              cGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTgg      >  1:3615995/1‑101 (MQ=255)
                                                                                               ggAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGt     <  2:4292563/101‑1 (MQ=255)
                                                                                               ggAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGt     >  1:381868/1‑101 (MQ=255)
                                                                                                gAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTgggc    >  2:3032778/1‑99 (MQ=255)
                                                                                                gAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTc    <  2:2309932/101‑1 (MQ=255)
                                                                                                gAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTc    >  2:3229962/1‑101 (MQ=255)
                                                                                                 aaGCCCCCCCCCCGGCCGCGCGGCGGATCTCGCCCCGGGGGGCGCTCCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCGTCGGGCTTTggcag   >  2:3824228/1‑98 (MQ=255)
                                                                                                  aGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCgg  <  1:4266127/101‑1 (MQ=255)
                                                                                                    |                                                                                                   
GCAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCCGGAGCGAACCGGAAGCCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCGG  >  NC_014034/3596843‑3597041

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG