Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 408,487 Δ1 bp 100% intergenic (+105/+43) glnA1 → / ← dapA glutamine synthetase/dihydrodipicolinate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034408,4870A→.100.0% 542.8 154intergenic (+105/+43)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (108/45):  ref base (0/1):  total (108/46)

Only 100 of 173 total aligned reads displayed.

CTGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGG....G.....GC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408387‑408571
                                                                                                    |                                                                                                    
cggggggCTCTCCCCCAAGCCCCCGGGGATATTTGGGGCAAGATAAACCCGTTTCCCTTCTTCTGCCCGCAAATACCCGGGGGGGAGGCTTGCTGAAAGCa                                                                                                      <  1:4230648/99‑1 (MQ=16)
cgggggcCTCTCCCCCCAGCCCCCGGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCa                                                                                                      <  1:2442465/99‑1 (MQ=25)
cTGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGGAAGATGAAGCCGATGTCCTTCATCTTTCCGCAAATATCCCCGGGGGGGGCTTGCTGAAACCa                                                                                                      >  2:1989352/1‑101 (MQ=25)
 tGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCCTCTTGCCGCAAATATCCCGGGGGGAGGGTTGCTGAAAGCaa                                                                                                     >  2:825357/1‑101 (MQ=255)
 tGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaa                                                                                                     >  1:2777807/1‑101 (MQ=255)
   ggggCTCTGCCCCCAGCCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaaga                                                                                                   <  2:749009/101‑3 (MQ=255)
   ggggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaaga                                                                                                   >  1:3547312/1‑99 (MQ=255)
   ggggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaaga                                                                                                   >  2:2992851/1‑99 (MQ=255)
   gggcCTCTGCCCCCGGCCCCCCGGGATATTGGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCaaga                                                                                                   <  1:959866/101‑3 (MQ=21)
        tctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCCGAAAGCaacacgggg                                                                                              >  2:242960/1‑94 (MQ=255)
          tGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACgggg....g.....g                                                                                  <  2:3073394/101‑1 (MQ=255)
           gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gc                                                                                 >  2:2276345/1‑101 (MQ=255)
             ccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcag                                                                               >  2:746743/1‑99 (MQ=255)
              ccaagccccccGGGTTATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcaga                                                                              <  1:1264218/95‑4 (MQ=25)
               ccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagag                                                                             >  2:4132563/1‑97 (MQ=255)
                 agcccccgGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcc                                                                           <  2:4039632/98‑7 (MQ=25)
                 agccccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcc                                                                           <  2:1697371/98‑7 (MQ=25)
                 aGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCCGAAAGC.AACACGGGG....G.....Gcagagcc                                                                           >  2:3588934/1‑95 (MQ=25)
                  gACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagccc                                                                          >  2:4568544/1‑94 (MQ=25)
                   aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcccc                                                                         >  1:1065102/1‑93 (MQ=25)
                   aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcccc                                                                         <  1:2679001/101‑9 (MQ=25)
                   aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcccc                                                                         >  2:487519/1‑93 (MQ=25)
                    cccccGGGATATTTGCGGCAAGATGAAGCCGATGTTCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagccccc                                                                        >  1:4172146/1‑92 (MQ=25)
                    cccccGGACTATTACCAGAAAGATCAACCGGATGCCCTTCATCTTACCGCAAATGTCCCGGGGGGAGGCTTGCTAAAAGCAAA.AGGGGG....G.....Gcagagccccc                                                                        <  2:4317961/101‑10 (MQ=255)
                     ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcccccc                                                                       <  1:2206378/101‑11 (MQ=25)
                                 tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGcc                                                         <  1:1908193/101‑1 (MQ=21)
                                  gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCa                                                        >  1:3848788/1‑101 (MQ=21)
                                  gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCa                                                        >  2:2159111/1‑101 (MQ=21)
                                   cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCaa                                                       >  1:2749363/1‑101 (MQ=21)
                                     gCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCaaaa                                                     <  2:4497507/101‑1 (MQ=21)
                                       aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAgg                                                   <  1:4496811/101‑1 (MQ=21)
                                       aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAgg                                                   <  2:1790302/101‑1 (MQ=21)
                                       aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAgg                                                   >  1:674154/1‑101 (MQ=21)
                                        aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGga                                                  >  1:2223795/1‑101 (MQ=21)
                                        aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTCGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGga                                                  <  2:4448927/101‑1 (MQ=21)
                                          aTGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGgacc                                                >  2:1816819/1‑101 (MQ=21)
                                           tGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGgaccg                                               >  2:781156/1‑101 (MQ=21)
                                             agcccGGTGTCTTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGgaccgac                                             <  2:40315/98‑1 (MQ=18)
                                             aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGgaccgac                                             <  2:258401/101‑1 (MQ=21)
                                              aGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGgaccgacc                                            <  1:1537906/101‑1 (MQ=21)
                                               gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGGACAGGTCGCCAAAAGGACCGACCt                                           >  2:1386654/1‑101 (MQ=18)
                                               gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCt                                           <  1:2749404/101‑1 (MQ=21)
                                               gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCt                                           >  1:241500/1‑101 (MQ=21)
                                               gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....GCAGAGAC.CC.CCGCACAGGTCGCCAAAAGgaccgacat                                           >  1:1521024/1‑99 (MQ=21)
                                                ccGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....GCAGAG.CGCC.CCGCACAGGTCGCCAAAAGGACCGACCTc                                          <  1:4190236/101‑1 (MQ=21)
                                                 cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCa                                         >  1:100880/1‑101 (MQ=21)
                                                  gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        >  1:3223216/1‑101 (MQ=21)
                                                   aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       <  2:1406402/101‑1 (MQ=21)
                                                   aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       >  2:1563419/1‑101 (MQ=21)
                                                      tCCTTCATCTTGCCGCAAATTTCCCGGGGGGAGTTTTGCTGCAAGC.AAGACGGGGTGCAGA....GC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACTTCTGATCa                                    <  1:3407254/101‑1 (MQ=12)
                                                      tCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                    <  1:4497985/101‑1 (MQ=21)
                                                       ccTTCATCTTGCCGCAGATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  1:4075084/1‑101 (MQ=25)
                                                       ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  2:479483/1‑101 (MQ=21)
                                                        cTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                  >  1:4389455/1‑101 (MQ=21)
                                                         ttCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                                 >  2:1651569/1‑101 (MQ=21)
                                                          tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCCAAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:4010994/1‑101 (MQ=18)
                                                          tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:3182280/1‑101 (MQ=21)
                                                          tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:766393/1‑101 (MQ=21)
                                                           cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               >  1:4480274/1‑101 (MQ=21)
                                                            aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                              >  1:2837731/1‑101 (MQ=21)
                                                            aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                              >  1:1240321/1‑101 (MQ=21)
                                                             tCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                             >  2:4056323/1‑101 (MQ=21)
                                                             tCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                             >  2:4368198/1‑101 (MQ=21)
                                                              cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            >  2:1147166/1‑101 (MQ=25)
                                                              cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            >  2:2230083/1‑101 (MQ=25)
                                                              cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            <  1:1386509/101‑1 (MQ=25)
                                                                tGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....GCAGAG.C.CCGCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtg                          >  1:3921440/1‑101 (MQ=25)
                                                                 gCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtgt                         <  1:1794525/101‑1 (MQ=25)
                                                                   cGCAACTAGCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgacggg                       <  2:3470564/101‑6 (MQ=255)
                                                                   cGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTg                       >  2:1826721/1‑101 (MQ=25)
                                                                    gCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGc                      <  2:1137399/101‑1 (MQ=25)
                                                                     cAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                     >  2:4633618/1‑101 (MQ=25)
                                                                      aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  1:2987627/1‑101 (MQ=37)
                                                                      aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  1:4441865/1‑101 (MQ=37)
                                                                      aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  2:3814545/1‑101 (MQ=37)
                                                                       aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   <  2:2777609/101‑1 (MQ=255)
                                                                       aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  2:1368927/1‑101 (MQ=255)
                                                                        atatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcg                  >  2:3408732/1‑101 (MQ=255)
                                                                         tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCNNCNNCAGATCAGCCCGGCGTGTTGCATcgc                 >  1:3045995/1‑101 (MQ=255)
                                                                         tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgc                 >  1:4292572/1‑101 (MQ=255)
                                                                            cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc              <  2:3520928/101‑1 (MQ=255)
                                                                            cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc              >  1:3322444/1‑101 (MQ=255)
                                                                            cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc              <  2:3049990/101‑1 (MQ=255)
                                                                             ccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggcg             >  2:1922394/1‑101 (MQ=255)
                                                                              cGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGt            >  2:600178/1‑101 (MQ=255)
                                                                               ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:3346193/1‑101 (MQ=255)
                                                                               ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:3254146/1‑101 (MQ=255)
                                                                               ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:544094/1‑101 (MQ=255)
                                                                               ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  1:2616540/1‑101 (MQ=255)
                                                                               ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:1253382/1‑101 (MQ=255)
                                                                                gggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa          >  1:2126809/1‑101 (MQ=255)
                                                                                gggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa          >  2:1001673/1‑101 (MQ=255)
                                                                                 ggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa         <  2:2405812/101‑1 (MQ=255)
                                                                                   ggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCCTCGCGGCGTCAAgc       >  1:1019174/1‑101 (MQ=255)
                                                                                    gAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  1:2392474/1‑101 (MQ=255)
                                                                                    gAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  2:4294398/1‑101 (MQ=255)
                                                                                     aGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcgc     >  2:1304473/1‑101 (MQ=255)
                                                                                       gCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATAGCGGCGTCAAgagagg   >  2:264864/1‑96 (MQ=255)
                                                                                        cTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc  >  1:609187/1‑101 (MQ=255)
                                                                                        cTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc  >  1:51870/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CTGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGG....G.....GC.....C.CC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408387‑408571

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG