Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,464 Δ1 bp 3.8% intergenic (+67/+81) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4640G→. 3.8% 14.7 ‑3.1 ‑0.4183intergenic (+67/+81)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (73/101):  total (81/102)
Fisher's exact test strand distribution p-value = 4.43e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.75e-01

Only 100 of 201 total aligned reads displayed.

CGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGG  >  NC_014034/432365‑432564
                                                                                                     |                                                                                                      
cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAg                                                                                                        <  1:3930188/101‑1 (MQ=255)
  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGcc                                                                                                      <  2:3572371/101‑1 (MQ=255)
   gcGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                                                                     <  2:4442502/100‑1 (MQ=255)
   aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGT.ATTTTTAGCAAGATGGAAggac                                                                                                     >  2:833919/1‑98 (MQ=18)
   aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                                                                     <  2:1598431/101‑1 (MQ=255)
      cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATc                                                                                                  <  2:618995/101‑1 (MQ=255)
      cTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATc                                                                                                  <  2:1974829/101‑1 (MQ=255)
       tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATcc                                                                                                 <  2:3356647/101‑1 (MQ=255)
            gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                            >  1:1804970/1‑101 (MQ=255)
            gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCCAGATGAAAGCCGATCCGGGCt                                                                                            <  1:3433570/101‑1 (MQ=255)
             cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                           >  2:250936/1‑101 (MQ=255)
               gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                         <  2:2373731/101‑1 (MQ=255)
                cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                        >  2:4520429/1‑101 (MQ=255)
                 ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGGAAAATGAAAGACGGTCCggggcttggt                                                                                       >  1:38039/1‑94 (MQ=17)
                 ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                       <  2:3735403/101‑1 (MQ=255)
                  gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                      <  1:1638360/101‑1 (MQ=255)
                  gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                      <  1:2792080/101‑1 (MQ=255)
                   cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                     <  2:869401/101‑1 (MQ=255)
                       ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                                <  1:1954685/101‑1 (MQ=255)
                       ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                                <  2:1574779/101‑1 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                               <  1:2718539/101‑1 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                               <  1:333529/101‑1 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                               <  1:1474658/101‑1 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                               <  1:3752183/101‑1 (MQ=255)
                          cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.Gc                                                                            <  1:4167960/101‑1 (MQ=255)
                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.Gcc                                                                           <  1:68002/101‑1 (MQ=255)
                              tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCa                                                                        >  2:1021115/1‑101 (MQ=255)
                              tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAAATGAAAGACGGTCCGGGCTTCGTTTCATC.TT.GCCCCa                                                                        >  2:3248387/1‑101 (MQ=18)
                               gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaa                                                                       >  1:161997/1‑101 (MQ=255)
                                aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTAATTTAAGGAAAAATAAAACCGGACCGGGGCTTCGTTT.ATCTTT.GCCCCaa                                                                       >  2:3207103/1‑101 (MQ=2)
                                aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaaa                                                                      >  1:4534122/1‑101 (MQ=255)
                                aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaaa                                                                      <  2:1736919/101‑1 (MQ=255)
                                 ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGGTTCGTTTCATC.TT.GCCCCAAat                                                                     <  2:2939540/101‑1 (MQ=255)
                                 ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAat                                                                     <  2:2621547/101‑1 (MQ=255)
                                  gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAata                                                                    <  1:4201899/101‑1 (MQ=255)
                                  gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAata                                                                    <  2:3487981/101‑1 (MQ=255)
                                    cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATc                                                                  >  1:2735875/1‑101 (MQ=255)
                                     gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATcc                                                                 >  1:3722448/1‑101 (MQ=255)
                                     gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATcc                                                                 <  1:2185617/101‑1 (MQ=255)
                                     gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATcc                                                                 >  1:4312483/1‑101 (MQ=255)
                                     gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAG.ATTTGAGGGAAGATGAAAGCCGATCAGGGCTTCTTTTCATC.TT.CACCCAAAAATcc                                                                 >  1:3011440/1‑101 (MQ=18)
                                       gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttg                                                    >  1:2388947/4‑100 (MQ=2)
                                       gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttg                                                    >  1:3063551/4‑100 (MQ=2)
                                       gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCg                                                               <  1:19822/101‑1 (MQ=255)
                                        acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttgg                                                   >  1:2229861/3‑99 (MQ=2)
                                         cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttggc                                                  >  2:4559270/2‑98 (MQ=2)
                                         aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAATCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCggg                                                             >  2:4638799/1‑101 (MQ=255)
                                         aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCggg                                                             >  1:486387/1‑101 (MQ=255)
                                             gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTg                                                         <  2:1475165/101‑1 (MQ=255)
                                              ggCGCCGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGa                                                        <  2:757808/101‑1 (MQ=25)
                                                cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAt                                                      <  2:2824239/101‑1 (MQ=255)
                                                 ccTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAtt                                                     <  2:2867068/100‑1 (MQ=255)
                                                  cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAttt                                                    <  1:3269642/101‑1 (MQ=255)
                                                   tGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAtttt                                                   >  2:4164595/1‑101 (MQ=255)
                                                    gCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTg                                                  >  1:4387358/1‑101 (MQ=255)
                                                     ccccccGTCCTGCGGACCCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTgg                                                 <  2:4189086/101‑1 (MQ=255)
                                                       ccccGTCCTGCGGACTCCCCCCGGGAT.ATTTTAGGGAAGAAGAAAACCGATCCCGGGCTCGTTTCATC.TTGGCCCAAAAAATCCCGGGGGGGAGTTTTgcg                                                >  2:3973020/1‑99 (MQ=12)
                                                          cgtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTTgcgccg                                            >  1:157086/5‑99 (MQ=255)
                                                           gtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                           <  2:1144797/98‑1 (MQ=255)
                                                             ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                         >  1:1532915/2‑101 (MQ=255)
                                                             ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                         <  1:4351667/100‑1 (MQ=255)
                                                               tGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                       <  2:2013252/101‑1 (MQ=255)
                                                               tGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                       <  2:1628959/101‑1 (MQ=255)
                                                                gCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                      <  1:4290940/101‑1 (MQ=255)
                                                                  ggACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                    >  1:2011061/1‑101 (MQ=255)
                                                                   gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                   >  1:2293202/1‑101 (MQ=255)
                                                                   gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                   <  2:3018378/101‑1 (MQ=255)
                                                                   gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                   <  1:833995/101‑1 (MQ=255)
                                                                    aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                  <  2:389526/101‑1 (MQ=255)
                                                                    aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                  >  1:4662428/1‑101 (MQ=255)
                                                                      tCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                <  2:4289789/101‑1 (MQ=255)
                                                                      tCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                <  1:4639100/101‑1 (MQ=255)
                                                                       ccccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                               >  2:1787468/1‑101 (MQ=255)
                                                                        cccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                              <  2:2743097/101‑1 (MQ=255)
                                                                         ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                             >  2:3830828/1‑101 (MQ=255)
                                                                          cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCTTTTCATA.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGTGGCAg                            >  2:1509760/1‑101 (MQ=255)
                                                                          cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                            >  2:783530/1‑101 (MQ=255)
                                                                            cGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                          >  2:2521322/1‑101 (MQ=255)
                                                                             gggAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                         >  2:2174067/1‑101 (MQ=255)
                                                                              ggAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgcg                        <  1:4588656/101‑2 (MQ=255)
                                                                               gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                       >  1:4047097/1‑101 (MQ=255)
                                                                                at.atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                      <  1:2973486/101‑1 (MQ=255)
                                                                                at.atTTGAGGCAAGATGAAAACCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                      >  2:595535/1‑101 (MQ=255)
                                                                                 t.atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                     >  1:2873087/1‑101 (MQ=255)
                                                                                   atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                    <  1:1769233/101‑1 (MQ=255)
                                                                                    tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                   >  1:1184163/1‑101 (MQ=255)
                                                                                    tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                   >  1:1121381/1‑101 (MQ=255)
                                                                                     ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                  >  2:168727/1‑101 (MQ=255)
                                                                                        aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc               >  1:2836223/1‑101 (MQ=255)
                                                                                         ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc              <  2:332713/101‑1 (MQ=255)
                                                                                          gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg             >  2:2731098/1‑101 (MQ=255)
                                                                                            aaGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg           >  1:1025979/1‑101 (MQ=255)
                                                                                             aGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg          >  1:855383/1‑101 (MQ=255)
                                                                                              gaggAAAGCCGATCCGGGCTTCGTTTCATC.TT.TCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc         <  2:4400977/98‑1 (MQ=255)
                                                                                                  aaaGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg     >  2:2052682/1‑101 (MQ=255)
                                                                                                  aaaGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggagcgg     >  2:631590/1‑101 (MQ=255)
                                                                                                   aaGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc    >  1:1878708/1‑101 (MQ=255)
                                                                                                   aaGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc    >  2:1058500/1‑101 (MQ=255)
                                                                                                    aGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg   >  2:4251532/1‑101 (MQ=255)
                                                                                                    aGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg   <  2:3251377/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
CGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGG  >  NC_014034/432365‑432564

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG