Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,465 Δ1 bp 3.8% intergenic (+68/+80) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4650C→. 3.8% 14.0 ‑3.1 ‑0.4184intergenic (+68/+80)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (75/102):  total (81/103)
Fisher's exact test strand distribution p-value = 4.49e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.03e-01

Only 100 of 199 total aligned reads displayed.

GCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGC  >  NC_014034/432366‑432565
                                                                                                    |                                                                                                     
  gcGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                     <  2:4442502/100‑1 (MQ=255)
  aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                     <  2:2707178/101‑1 (MQ=255)
  aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                     <  2:3375059/101‑1 (MQ=255)
     cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                                  <  2:618995/101‑1 (MQ=255)
       ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                <  1:4309525/101‑1 (MQ=255)
       tgcgggggccgcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                <  2:2015319/99‑1 (MQ=18)
        tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                               <  1:1487165/101‑1 (MQ=255)
         cggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCggg                                                                                              <  1:4239376/101‑1 (MQ=255)
           ggggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                            <  1:853348/99‑1 (MQ=255)
           gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCGATCCGGGCt                                                                                            <  1:3433570/101‑1 (MQ=255)
               cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                        >  2:4520429/1‑101 (MQ=255)
               cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                        >  2:2929000/1‑101 (MQ=255)
                ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                       >  1:1629064/1‑101 (MQ=255)
                ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGGAAAATGAAAGACGGTCCggggcttggt                                                                                       >  1:38039/1‑94 (MQ=17)
                 gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                      <  1:2792080/101‑1 (MQ=255)
                  cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                     <  2:3050214/101‑1 (MQ=255)
                    ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                   <  1:1053233/101‑1 (MQ=255)
                    ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                   <  2:1129175/101‑1 (MQ=255)
                       cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                <  1:2718539/101‑1 (MQ=255)
                       cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                <  1:3752183/101‑1 (MQ=255)
                       cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                <  1:333529/101‑1 (MQ=255)
                        aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.g                                                                              <  1:4666643/101‑1 (MQ=255)
                         cgtcTGAGGCCGGGCAGGGGGGCGCTTCCCCCCGTCCTGGGGAGTGCCCCCGGGATATTTGTGGCAGCATGAAAGCCGATCCGGGCTTCGTTTCATCtttc                                                                              <  2:3847219/98‑3 (MQ=18)
                              gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAAGCAAGATGAAAGTCGATACGGGCTTCGTTTCATCTT.GCCCCaa                                                                        >  2:32480/1‑101 (MQ=18)
                                  ccGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAatat                                                                    <  2:1742254/101‑1 (MQ=255)
                                   cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATc                                                                   >  2:2813133/1‑101 (MQ=255)
                                   cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATc                                                                   >  1:2735875/1‑101 (MQ=255)
                                    gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATcc                                                                  >  1:3722448/1‑101 (MQ=255)
                                    gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAAATGAAAGCAGATCCGGGCTTCGTTTCATCTT.TCCCCAAatatac                                                                  >  1:1176229/1‑99 (MQ=18)
                                    gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAGATTTGAGGGAAGATGAAAGCCGATCAGGGCTTCTTTTCATCTT.CACCCAAAAATcc                                                                  >  1:3011440/1‑101 (MQ=18)
                                     ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTT.GCCCGAAATATCCCAGGGGTGAAtt                                                      >  1:1996360/5‑101 (MQ=2)
                                     ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTT.GCCCGAAATATCCCAGGGGTGAAtt                                                      >  1:4436668/5‑101 (MQ=2)
                                     ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATccc                                                                 <  2:351802/101‑1 (MQ=255)
                                      gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTT.GCCCGAAATATCCCAGGGGTGAAttg                                                     >  1:3063551/4‑100 (MQ=2)
                                      gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTT.GCCCGAAATATCCCAGGGGTGAAttg                                                     >  1:2388947/4‑100 (MQ=2)
                                      gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTT.GCCCGAAATATCCCAGGGGTGAAttg                                                     <  1:4617392/98‑2 (MQ=2)
                                      gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCg                                                                <  1:1585537/101‑1 (MQ=255)
                                        cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTT.GCCCGAAATATCCCAGGGGTGAAttggc                                                   >  2:4559270/2‑98 (MQ=2)
                                        aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAATCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCggg                                                              >  2:4638799/1‑101 (MQ=255)
                                        aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCGTCGTTTCATCTT.GCCCCAAATATCCCggg                                                              >  2:2142120/1‑101 (MQ=255)
                                         ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCgggg                                                             >  1:4644551/1‑101 (MQ=255)
                                           ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGt                                                           <  2:179948/101‑1 (MQ=255)
                                            gggCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTg                                                          <  1:503068/101‑1 (MQ=255)
                                             ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGa                                                         >  1:1890163/1‑101 (MQ=255)
                                             ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGa                                                         <  2:1673987/101‑1 (MQ=255)
                                             ggCGCCGCCCCCCGTCCTGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGa                                                         <  2:757808/101‑1 (MQ=25)
                                                gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAtt                                                      <  2:2827217/101‑1 (MQ=255)
                                                gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGGGAAtt                                                      >  1:750196/1‑101 (MQ=255)
                                                 cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAttt                                                     <  2:2282586/101‑1 (MQ=255)
                                                 cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAttt                                                     <  1:3269642/101‑1 (MQ=255)
                                                 cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAttt                                                     >  2:2511118/1‑101 (MQ=255)
                                                 cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGGGAAttt                                                     >  2:1537481/1‑101 (MQ=255)
                                                  tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAtttt                                                    >  2:4164595/1‑101 (MQ=255)
                                                  tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAtttt                                                    >  1:2219773/1‑101 (MQ=255)
                                                    ccccccGTCCTGCGGACCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTgg                                                  <  2:4189086/101‑1 (MQ=255)
                                                     cccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGgc                                                 >  2:2009999/1‑101 (MQ=255)
                                                      ccccGTCCTGCGGACTCCCCCCGGGATATTTTAGGGAAGAAGAAAACCGATCCCGGGCTCGTTTCATCTTGGCCCAAAAAATCCCGGGGGGGAGTTTTgcg                                                 >  2:3973020/1‑99 (MQ=12)
                                                          gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                            <  1:4540420/98‑1 (MQ=255)
                                                           tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                           <  2:161983/99‑1 (MQ=255)
                                                           tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                           <  2:1712181/99‑1 (MQ=255)
                                                           tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                           >  2:3334519/3‑101 (MQ=255)
                                                            ccTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGGGGATTTTGGCGCAGCCa                                          >  2:1576406/2‑101 (MQ=255)
                                                            ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                          >  1:1863663/2‑101 (MQ=255)
                                                             cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                         >  2:2972446/1‑101 (MQ=255)
                                                                   aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                   >  1:1345510/1‑101 (MQ=255)
                                                                   aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                   >  2:3787760/1‑101 (MQ=255)
                                                                     tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                 <  2:4289789/101‑1 (MQ=255)
                                                                        ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                              <  2:846274/101‑1 (MQ=255)
                                                                         cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                             >  2:783530/1‑101 (MQ=255)
                                                                          ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGGTGCGTTTCATCTT.GCCCCAAATGTGCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                            <  1:155314/101‑1 (MQ=25)
                                                                          ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                            <  1:899342/101‑1 (MQ=255)
                                                                          ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                            <  1:197290/101‑1 (MQ=255)
                                                                           cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                           >  1:2282133/1‑101 (MQ=255)
                                                                           cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTGCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                           >  2:2377296/1‑101 (MQ=255)
                                                                              gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                        <  1:4382486/101‑1 (MQ=255)
                                                                               atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                       <  1:2973486/101‑1 (MQ=255)
                                                                               atatTTGAGGCAAGATGAAAACCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                       >  2:595535/1‑101 (MQ=255)
                                                                               atatTGGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                       <  2:273260/101‑1 (MQ=255)
                                                                                tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                      >  1:2873087/1‑101 (MQ=255)
                                                                                    tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                  >  2:1285456/1‑101 (MQ=255)
                                                                                     gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                 >  1:208632/1‑101 (MQ=255)
                                                                                      aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                >  1:2836223/1‑101 (MQ=255)
                                                                                      aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                >  2:3631547/1‑101 (MQ=255)
                                                                                        gCAGGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              <  1:4164883/101‑1 (MQ=255)
                                                                                        gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              <  2:943700/101‑1 (MQ=255)
                                                                                        gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              <  1:4324902/101‑1 (MQ=255)
                                                                                         cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc             >  2:3281942/1‑101 (MQ=255)
                                                                                          aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg            >  1:1025979/1‑101 (MQ=255)
                                                                                            gaggAAAGCCGATCCGGGCTTCGTTTCATCTT.TCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc          <  2:4400977/98‑1 (MQ=255)
                                                                                             aTGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcg         >  1:1769544/1‑101 (MQ=255)
                                                                                              tGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc        >  2:3250078/1‑101 (MQ=255)
                                                                                               gAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg       >  1:1659956/1‑101 (MQ=255)
                                                                                                aaaGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg      >  2:255172/1‑101 (MQ=255)
                                                                                                aaaGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg      <  2:1039238/101‑1 (MQ=255)
                                                                                                aaaGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg      >  1:4188969/1‑101 (MQ=255)
                                                                                                aaaGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggagcgg      >  2:631590/1‑101 (MQ=255)
                                                                                                  aGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg    >  2:4251532/1‑101 (MQ=255)
                                                                                                  aGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg    >  2:1497541/1‑101 (MQ=255)
                                                                                                   gCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAACATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcgg   <  1:3973305/101‑1 (MQ=255)
                                                                                                    ccGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc  >  2:3907041/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
GCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGC  >  NC_014034/432366‑432565

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG