Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,466 Δ1 bp 3.8% intergenic (+69/+79) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4660C→. 3.8% 14.0 ‑3.1 ‑0.6184intergenic (+69/+79)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (74/102):  total (81/103)
Fisher's exact test strand distribution p-value = 4.44e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.69e-01

Only 100 of 198 total aligned reads displayed.

CACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGC  >  NC_014034/432367‑432565
                                                                                                     |                                                                                                    
cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGcc                                                                                                      <  2:3572371/101‑1 (MQ=255)
 aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                                                                     <  2:1598431/101‑1 (MQ=255)
    cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATc                                                                                                  <  2:618995/101‑1 (MQ=255)
    cTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATc                                                                                                  <  2:1974829/101‑1 (MQ=255)
     tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATcc                                                                                                 <  2:3356647/101‑1 (MQ=255)
      ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                <  1:4309525/101‑1 (MQ=255)
       tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                               <  1:1487165/101‑1 (MQ=255)
        cggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCggg                                                                                              <  1:4239376/101‑1 (MQ=255)
          ggggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                            <  1:853348/99‑1 (MQ=255)
          gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCCAGATGAAAGCCGATCCGGGCt                                                                                            <  1:3433570/101‑1 (MQ=255)
           cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                           >  2:250936/1‑101 (MQ=255)
            ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                          <  2:4309508/101‑1 (MQ=255)
             gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                         <  2:2373731/101‑1 (MQ=255)
               ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                       >  1:1629064/1‑101 (MQ=255)
               ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGGAAAATGAAAGACGGTCCggggcttggt                                                                                       >  1:38039/1‑94 (MQ=17)
               ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                       <  2:3735403/101‑1 (MQ=255)
                gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                      <  1:2792080/101‑1 (MQ=255)
                   ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                   <  1:1053233/101‑1 (MQ=255)
                   ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                   <  2:1129175/101‑1 (MQ=255)
                     ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                                <  2:1574779/101‑1 (MQ=255)
                      cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                               <  1:3752183/101‑1 (MQ=255)
                      cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                               <  1:2718539/101‑1 (MQ=255)
                      cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                               <  1:333529/101‑1 (MQ=255)
                       aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTg                                                                              <  1:4666643/101‑1 (MQ=255)
                          ccTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGccc                                                                           >  2:3957674/1‑101 (MQ=255)
                            tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAAATGAAAGACGGTCCGGGCTTCGTTTCATC.TTGCCCCa                                                                         >  2:3248387/1‑101 (MQ=18)
                              aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTAATTTAAGGAAAAATAAAACCGGACCGGGGCTTCGTTT.ATCTTTGCCCCaa                                                                        >  2:3207103/1‑101 (MQ=2)
                              aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                                                       <  2:1736919/101‑1 (MQ=255)
                               ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGGTTCGTTTCATC.TTGCCCCAAat                                                                      <  2:2939540/101‑1 (MQ=255)
                               ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                                                      <  2:2621547/101‑1 (MQ=255)
                                gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                     <  2:3487981/101‑1 (MQ=255)
                                  cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATc                                                                   >  1:2735875/1‑101 (MQ=255)
                                   gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                  <  2:4264487/101‑1 (MQ=255)
                                   gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                  >  1:3722448/1‑101 (MQ=255)
                                   gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAAATGAAAGCAGATCCGGGCTTCGTTTCATC.TTTCCCCAAatatac                                                                  >  1:1176229/1‑99 (MQ=18)
                                   gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAG.ATTTGAGGGAAGATGAAAGCCGATCAGGGCTTCTTTTCATC.TTCACCCAAAAATcc                                                                  >  1:3011440/1‑101 (MQ=18)
                                    ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAtt                                                      >  1:1996360/5‑101 (MQ=2)
                                    ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAtt                                                      >  1:4436668/5‑101 (MQ=2)
                                     gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                                     >  1:2388947/4‑100 (MQ=2)
                                     gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                                     >  1:3063551/4‑100 (MQ=2)
                                     gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                                     <  1:4617392/98‑2 (MQ=2)
                                     gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCg                                                                <  1:1585537/101‑1 (MQ=255)
                                      cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCgg                                                               >  2:4588357/1‑101 (MQ=255)
                                        ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCgggg                                                             >  1:4644551/1‑101 (MQ=255)
                                           gggCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                                          <  1:503068/101‑1 (MQ=255)
                                           gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                                          <  2:1475165/101‑1 (MQ=255)
                                            ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGa                                                         >  1:1890163/1‑101 (MQ=255)
                                            ggCGCCGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGa                                                         <  2:757808/101‑1 (MQ=25)
                                              cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                                                       <  2:2824239/101‑1 (MQ=255)
                                               gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAAtt                                                      >  1:750196/1‑101 (MQ=255)
                                               ccTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtt                                                      <  2:2867068/100‑1 (MQ=255)
                                                cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                                     <  1:3269642/101‑1 (MQ=255)
                                                 tGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtttt                                                    >  1:2219773/1‑101 (MQ=255)
                                                         gtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                            <  1:4540420/98‑1 (MQ=255)
                                                           ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                          <  2:4066967/100‑1 (MQ=255)
                                                           ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                          >  1:1863663/2‑101 (MQ=255)
                                                             tGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                        <  2:1628959/101‑1 (MQ=255)
                                                             tGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                        <  2:2013252/101‑1 (MQ=255)
                                                                 gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                    <  2:3018378/101‑1 (MQ=255)
                                                                 gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                    >  2:4551371/1‑101 (MQ=255)
                                                                  aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                   >  1:1345510/1‑101 (MQ=255)
                                                                  aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                   <  2:389526/101‑1 (MQ=255)
                                                                     ccccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                                >  2:1787468/1‑101 (MQ=255)
                                                                      cccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                               <  2:2743097/101‑1 (MQ=255)
                                                                       ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                              <  2:846274/101‑1 (MQ=255)
                                                                       ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                              >  2:3830828/1‑101 (MQ=255)
                                                                        cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCTTTTCATA.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGTGGCAg                             >  2:1509760/1‑101 (MQ=255)
                                                                        cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                             >  2:783530/1‑101 (MQ=255)
                                                                         ccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGGTGCGTTTCATC.TTGCCCCAAATGTGCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                            <  1:155314/101‑1 (MQ=25)
                                                                         ccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                            <  1:197290/101‑1 (MQ=255)
                                                                         ccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                            <  1:899342/101‑1 (MQ=255)
                                                                          cGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                           >  1:2282133/1‑101 (MQ=255)
                                                                          cGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                           >  2:2521322/1‑101 (MQ=255)
                                                                           gggAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                          >  2:2174067/1‑101 (MQ=255)
                                                                             gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                        <  1:4382486/101‑1 (MQ=255)
                                                                             gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                        >  2:442442/1‑101 (MQ=255)
                                                                              at.atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                       <  1:2973486/101‑1 (MQ=255)
                                                                              at.atTTGAGGCAAGATGAAAACCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                       >  2:595535/1‑101 (MQ=255)
                                                                               t.atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                      >  1:2873087/1‑101 (MQ=255)
                                                                                 atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                     <  2:419522/101‑1 (MQ=255)
                                                                                   ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                   >  2:168727/1‑101 (MQ=255)
                                                                                   ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                   >  2:4479727/1‑101 (MQ=255)
                                                                                    tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                  >  2:1285456/1‑101 (MQ=255)
                                                                                     gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                 >  1:208632/1‑101 (MQ=255)
                                                                                      aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                >  1:2836223/1‑101 (MQ=255)
                                                                                       ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc               <  2:332713/101‑1 (MQ=255)
                                                                                        gCAGGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              <  1:4164883/101‑1 (MQ=255)
                                                                                        gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              <  2:943700/101‑1 (MQ=255)
                                                                                        gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              <  2:4167242/101‑1 (MQ=255)
                                                                                        gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              >  2:2731098/1‑101 (MQ=255)
                                                                                        gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              <  1:4324902/101‑1 (MQ=255)
                                                                                          aaGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg            >  1:1025979/1‑101 (MQ=255)
                                                                                             aTGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcg         >  1:1769544/1‑101 (MQ=255)
                                                                                               gAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg       >  1:1659956/1‑101 (MQ=255)
                                                                                                aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg      >  2:2052682/1‑101 (MQ=255)
                                                                                                aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg      <  2:1039238/101‑1 (MQ=255)
                                                                                                aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg      >  1:4188969/1‑101 (MQ=255)
                                                                                                aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggagcgg      >  2:631590/1‑101 (MQ=255)
                                                                                                  aGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg    <  2:3251377/101‑1 (MQ=255)
                                                                                                   gCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAACATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcgg   <  1:3973305/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGC  >  NC_014034/432367‑432565

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG