Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,469 Δ1 bp 3.8% intergenic (+72/+76) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4690T→. 3.8% 12.5 ‑3.0 ‑0.5185intergenic (+72/+76)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (79/99):  total (85/100)
Fisher's exact test strand distribution p-value = 4.93e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.45e-01

Only 100 of 197 total aligned reads displayed.

CTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCG  >  NC_014034/432371‑432568
                                                                                                   |                                                                                                    
cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                                     <  2:618995/101‑1 (MQ=255)
  tgcgggggccgcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                   <  2:2015319/99‑1 (MQ=18)
   tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                                  <  1:1487165/101‑1 (MQ=255)
      gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                               >  1:1804970/1‑101 (MQ=255)
      gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCCAGATGAAAGCCGATCCGGGCt                                                                                               <  1:3433570/101‑1 (MQ=255)
          cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                           >  2:2929000/1‑101 (MQ=255)
            gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                         <  1:1638360/101‑1 (MQ=255)
            gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                         <  1:2792080/101‑1 (MQ=255)
             cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                        <  2:3050214/101‑1 (MQ=255)
               ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                      <  1:1053233/101‑1 (MQ=255)
                 ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                    <  2:1574779/101‑1 (MQ=255)
                 ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                    <  1:1954685/101‑1 (MQ=255)
                  cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                   <  1:3741999/101‑1 (MQ=255)
                  cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                   <  1:333529/101‑1 (MQ=255)
                  cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                   <  1:2718539/101‑1 (MQ=255)
                    cgtcTGAGGCCGGGCAGGGGGGCGCTTCCCCCCGTCCTGGGGAGTGCCCCCGGGATATTTGTGGCAGCATGAAAGCCGATCCGGGCTTCGTTTCATCtttc                                                                                 <  2:3847219/98‑3 (MQ=18)
                    cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.Gc                                                                                <  1:4167960/101‑1 (MQ=255)
                     gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.Gcc                                                                               <  1:68002/101‑1 (MQ=255)
                        tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCa                                                                            >  2:1021115/1‑101 (MQ=255)
                         gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCaa                                                                           >  1:161997/1‑101 (MQ=255)
                         gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAAGCAAGATGAAAGTCGATACGGGCTTCGTTTCATCTT.GCCCCaa                                                                           >  2:32480/1‑101 (MQ=18)
                          aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCaaa                                                                          >  1:4534122/1‑101 (MQ=255)
                           ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAat                                                                         <  2:2621547/101‑1 (MQ=255)
                            gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAata                                                                        <  1:4201899/101‑1 (MQ=255)
                            gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAata                                                                        <  2:3487981/101‑1 (MQ=255)
                             ccGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAatat                                                                       <  2:1742254/101‑1 (MQ=255)
                              cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATc                                                                      >  1:2735875/1‑101 (MQ=255)
                              cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATc                                                                      >  2:2813133/1‑101 (MQ=255)
                               gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATcc                                                                     <  1:2185617/101‑1 (MQ=255)
                               gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATcc                                                                     >  1:4312483/1‑101 (MQ=255)
                               gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAAATGAAAGCAGATCCGGGCTTCGTTTCATCTT.TCCCCAAatatac                                                                     >  1:1176229/1‑99 (MQ=18)
                               gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAGATTTGAGGGAAGATGAAAGCCGATCAGGGCTTCTTTTCATCTT.CACCCAAAAATcc                                                                     >  1:3011440/1‑101 (MQ=18)
                                 gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTT.GCCCGAAATATCCCAGGGGTGAAttg                                                        >  1:3063551/4‑100 (MQ=2)
                                 gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCg                                                                   <  1:19822/101‑1 (MQ=255)
                                  cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCgg                                                                  >  2:4588357/1‑101 (MQ=255)
                                  acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTT.GCCCGAAATATCCCAGGGGTGAAttgg                                                       >  1:2229861/3‑99 (MQ=2)
                                   aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCggg                                                                 >  1:486387/1‑101 (MQ=255)
                                   aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCGTCGTTTCATCTT.GCCCCAAATATCCCggg                                                                 >  2:2142120/1‑101 (MQ=255)
                                      ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGt                                                              <  2:179948/101‑1 (MQ=255)
                                       gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTg                                                             <  2:1475165/101‑1 (MQ=255)
                                        ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGa                                                            <  2:1673987/101‑1 (MQ=255)
                                        ggCGCCGCCCCCCGTCCTGCGGCCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGa                                                            <  2:757808/101‑1 (MQ=25)
                                           gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAtt                                                         <  2:2827217/101‑1 (MQ=255)
                                            cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAttt                                                        >  2:2511118/1‑101 (MQ=255)
                                            cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAttt                                                        <  1:3269642/101‑1 (MQ=255)
                                            cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAttt                                                        <  2:2282586/101‑1 (MQ=255)
                                             tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAAtttt                                                       >  2:4164595/1‑101 (MQ=255)
                                              gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTg                                                      >  1:4387358/1‑101 (MQ=255)
                                               ccccccGTCCTGCGGACCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTgg                                                     <  2:4189086/101‑1 (MQ=255)
                                                cccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGgc                                                    >  2:2009999/1‑101 (MQ=255)
                                                 ccccGTCCTGCGGACTCCCCCCGGGATATTTTAGGGAAGAAGAAAACCGATCCCGGGCTCGTTTCATCTTGGCCCAAAAAATCCCGGGGGGGAGTTTTgcg                                                    >  2:3973020/1‑99 (MQ=12)
                                                    cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTTgcgccg                                                >  1:157086/5‑99 (MQ=255)
                                                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                               <  2:1144797/98‑1 (MQ=255)
                                                      tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                              <  2:1712181/99‑1 (MQ=255)
                                                      tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                              <  2:161983/99‑1 (MQ=255)
                                                      tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                              >  2:3334519/3‑101 (MQ=255)
                                                       ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                             <  1:4351667/100‑1 (MQ=255)
                                                        cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                            >  2:2972446/1‑101 (MQ=255)
                                                          gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                          <  1:4290940/101‑1 (MQ=255)
                                                            ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                        >  1:2011061/1‑101 (MQ=255)
                                                             gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                       >  2:4551371/1‑101 (MQ=255)
                                                             gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                       >  1:2293202/1‑101 (MQ=255)
                                                             gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                       <  1:833995/101‑1 (MQ=255)
                                                              aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                      >  2:3787760/1‑101 (MQ=255)
                                                              aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                      >  1:1345510/1‑101 (MQ=255)
                                                              aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                      >  1:4662428/1‑101 (MQ=255)
                                                                tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                    <  2:4289789/101‑1 (MQ=255)
                                                                tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                    <  1:2418742/101‑1 (MQ=255)
                                                                tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                    <  1:4639100/101‑1 (MQ=255)
                                                                   ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                 <  2:846274/101‑1 (MQ=255)
                                                                    cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCTTTTCATATT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGTGGCAg                                >  2:1509760/1‑101 (MQ=255)
                                                                    cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                >  2:783530/1‑101 (MQ=255)
                                                                      cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                              >  2:2521322/1‑101 (MQ=255)
                                                                      cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTGCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                              >  2:2377296/1‑101 (MQ=255)
                                                                        ggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgcg                            <  1:4588656/101‑2 (MQ=255)
                                                                         gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                           >  1:4047097/1‑101 (MQ=255)
                                                                          atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                          <  1:2973486/101‑1 (MQ=255)
                                                                          atatTTGAGGCAAGATGAAAACCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                          >  2:595535/1‑101 (MQ=255)
                                                                          atatTGGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                          <  2:273260/101‑1 (MQ=255)
                                                                           tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                         >  1:2873087/1‑101 (MQ=255)
                                                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                        >  1:3816961/1‑101 (MQ=255)
                                                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                        <  1:1769233/101‑1 (MQ=255)
                                                                              ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                      >  2:4479727/1‑101 (MQ=255)
                                                                              ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                      <  1:3757144/101‑1 (MQ=255)
                                                                                 aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                   >  2:3631547/1‑101 (MQ=255)
                                                                                 aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                   >  1:2836223/1‑101 (MQ=255)
                                                                                   gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                 <  2:943700/101‑1 (MQ=255)
                                                                                    cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc                >  2:3281942/1‑101 (MQ=255)
                                                                                     aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg               >  1:1025979/1‑101 (MQ=255)
                                                                                      aGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg              >  1:855383/1‑101 (MQ=255)
                                                                                       gaggAAAGCCGATCCGGGCTTCGTTTCATCTT.TCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc             <  2:4400977/98‑1 (MQ=255)
                                                                                         tGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc           >  2:3250078/1‑101 (MQ=255)
                                                                                           aaaGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggagcgg         >  2:631590/1‑101 (MQ=255)
                                                                                            aaGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc        >  2:1058500/1‑101 (MQ=255)
                                                                                            aaGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc        >  1:1878708/1‑101 (MQ=255)
                                                                                             aGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg       >  2:4251532/1‑101 (MQ=255)
                                                                                               ccGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc     >  2:3907041/1‑101 (MQ=255)
                                                                                                 gATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc   >  1:3873013/1‑101 (MQ=255)
                                                                                                  aTCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCTgcggcgcggcggcgcg  >  1:1548959/1‑101 (MQ=255)
                                                                                                  aTCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCACCccgacgcggcggagcg  >  1:3715506/1‑101 (MQ=21)
                                                                                                   |                                                                                                    
CTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCG  >  NC_014034/432371‑432568

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG