Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,471 Δ1 bp 3.9% intergenic (+74/+74) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4710C→. 3.9% 13.9 ‑2.9 ‑0.2180intergenic (+74/+74)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (79/94):  total (85/95)
Fisher's exact test strand distribution p-value = 5.33e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.13e-01

Only 100 of 198 total aligned reads displayed.

TTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCG  >  NC_014034/432372‑432571
                                                                                                     |                                                                                                     
tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATcc                                                                                                       <  2:3356647/101‑1 (MQ=255)
 tgcgggggccgcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                      <  2:2015319/99‑1 (MQ=18)
  tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCgg                                                                                                     <  1:1487165/101‑1 (MQ=255)
     gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                                  >  1:1804970/1‑101 (MQ=255)
     gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCCAGATGAAAGCCGATCCGGGCt                                                                                                  <  1:3433570/101‑1 (MQ=255)
      cggcgggggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                                 <  1:2845501/101‑1 (MQ=255)
       ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                                <  2:4309508/101‑1 (MQ=255)
         cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                              >  2:4520429/1‑101 (MQ=255)
         cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTATGGCAAGATGAAAGGCCATCCCGGCTTCGt                                                                                              >  1:273292/1‑101 (MQ=14)
          ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                             <  2:3735403/101‑1 (MQ=255)
           gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                            <  1:1638360/101‑1 (MQ=255)
              ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                         <  1:1053233/101‑1 (MQ=255)
              ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                         <  2:1129175/101‑1 (MQ=255)
                ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                                      <  1:1954685/101‑1 (MQ=255)
                ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                                      <  2:1574779/101‑1 (MQ=255)
                 cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                                     <  1:3741999/101‑1 (MQ=255)
                 cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                                     <  1:333529/101‑1 (MQ=255)
                   cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGc                                                                                   <  1:4167960/101‑1 (MQ=255)
                    gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                                                                  <  1:68002/101‑1 (MQ=255)
                     ccTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGccc                                                                                 >  2:3957674/1‑101 (MQ=255)
                       tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                                                               >  2:1021115/1‑101 (MQ=255)
                       tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                                                               <  1:3006872/101‑1 (MQ=255)
                       tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAAATGAAAGACGGTCCGGGCTTCGTTTCATC.TTGCCCCa                                                                               >  2:3248387/1‑101 (MQ=18)
                        gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                                                              >  1:161997/1‑101 (MQ=255)
                         aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTAATTTAAGGAAAAATAAAACCGGACCGGGGCTTCGTTT.ATCTTTGCCCCaa                                                                              >  2:3207103/1‑101 (MQ=2)
                         aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                                                             >  1:4534122/1‑101 (MQ=255)
                          ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGGTTCGTTTCATC.TTGCCCCAAat                                                                            <  2:2939540/101‑1 (MQ=255)
                           gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                           >  2:616582/1‑101 (MQ=255)
                           gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                           <  1:4201899/101‑1 (MQ=255)
                            ccGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAatat                                                                          <  2:1742254/101‑1 (MQ=255)
                              gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                        >  1:4312483/1‑101 (MQ=255)
                              gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                        <  2:4264487/101‑1 (MQ=255)
                              gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                        <  1:2185617/101‑1 (MQ=255)
                              gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAAATGAAAGCAGATCCGGGCTTCGTTTCATC.TTTCCCCAAatatac                                                                        >  1:1176229/1‑99 (MQ=18)
                               ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATccc                                                                       <  2:351802/101‑1 (MQ=255)
                                gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                                           >  1:3063551/4‑100 (MQ=2)
                                gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                                           >  1:2388947/4‑100 (MQ=2)
                                gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCg                                                                      <  1:19822/101‑1 (MQ=255)
                                 acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttgg                                                          >  1:2229861/3‑99 (MQ=2)
                                  cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttggc                                                         >  2:4559270/2‑98 (MQ=2)
                                  aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAATCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggg                                                                    >  2:4638799/1‑101 (MQ=255)
                                  aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggg                                                                    >  1:486387/1‑101 (MQ=255)
                                  aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCGTCGTTTCATC.TTGCCCCAAATATCCCggg                                                                    >  2:2142120/1‑101 (MQ=255)
                                     ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGt                                                                 <  2:179948/101‑1 (MQ=255)
                                      gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                                                <  2:1475165/101‑1 (MQ=255)
                                       ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGa                                                               <  2:1673987/101‑1 (MQ=255)
                                       ggCGCCGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGa                                                               <  2:757808/101‑1 (MQ=25)
                                         ggcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                                                             <  1:2929229/100‑1 (MQ=255)
                                         cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                                                             <  2:2824239/101‑1 (MQ=255)
                                          ccTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtt                                                            <  2:2867068/100‑1 (MQ=255)
                                           cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                                           >  2:2511118/1‑101 (MQ=255)
                                           cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                                           <  1:3269642/101‑1 (MQ=255)
                                           cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                                           <  2:2282586/101‑1 (MQ=255)
                                             gCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTg                                                         >  1:4387358/1‑101 (MQ=255)
                                               cccccGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGgc                                                       >  2:2009999/1‑101 (MQ=255)
                                                   cgtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTTgcgccg                                                   >  1:157086/5‑99 (MQ=255)
                                                     tccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                                 <  2:1712181/99‑1 (MQ=255)
                                                     tccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                                 <  2:161983/99‑1 (MQ=255)
                                                      ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                <  2:4066967/100‑1 (MQ=255)
                                                      ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                <  1:4351667/100‑1 (MQ=255)
                                                         gCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                             <  1:4290940/101‑1 (MQ=255)
                                                           ggACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                           >  1:2011061/1‑101 (MQ=255)
                                                            gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                          <  2:3018378/101‑1 (MQ=255)
                                                            gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                          >  1:2293202/1‑101 (MQ=255)
                                                            gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                          <  1:2740740/101‑1 (MQ=255)
                                                            gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                          <  1:833995/101‑1 (MQ=255)
                                                             aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                         <  2:389526/101‑1 (MQ=255)
                                                             aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                         >  1:4662428/1‑101 (MQ=255)
                                                             aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                         >  1:1345510/1‑101 (MQ=255)
                                                               tCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                       <  1:4639100/101‑1 (MQ=255)
                                                                 cccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                     <  2:2743097/101‑1 (MQ=255)
                                                                  ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                    >  2:3830828/1‑101 (MQ=255)
                                                                  ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                    <  2:846274/101‑1 (MQ=255)
                                                                   cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCTTTTCATA.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGTGGCAg                                   >  2:1509760/1‑101 (MQ=255)
                                                                   cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                   >  2:783530/1‑101 (MQ=255)
                                                                     cGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTGCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                 >  2:2377296/1‑101 (MQ=255)
                                                                       ggAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgcg                               <  1:4588656/101‑2 (MQ=255)
                                                                        gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                              >  2:442442/1‑101 (MQ=255)
                                                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                           <  2:419522/101‑1 (MQ=255)
                                                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                           >  1:3816961/1‑101 (MQ=255)
                                                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                           <  1:2813349/101‑1 (MQ=255)
                                                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                           <  1:1769233/101‑1 (MQ=255)
                                                                              ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                         <  1:3757144/101‑1 (MQ=255)
                                                                               tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                        >  2:1285456/1‑101 (MQ=255)
                                                                                  ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                     <  2:332713/101‑1 (MQ=255)
                                                                                   gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                    <  2:943700/101‑1 (MQ=255)
                                                                                   gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                    <  2:4167242/101‑1 (MQ=255)
                                                                                   gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                    >  2:2731098/1‑101 (MQ=255)
                                                                                     aaGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                  >  1:1025979/1‑101 (MQ=255)
                                                                                      aGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                 >  1:855383/1‑101 (MQ=255)
                                                                                           aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg            >  2:255172/1‑101 (MQ=255)
                                                                                           aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg            <  2:1039238/101‑1 (MQ=255)
                                                                                           aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggagcgg            >  2:631590/1‑101 (MQ=255)
                                                                                            aaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc           >  1:1878708/1‑101 (MQ=255)
                                                                                             aGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg          <  2:3251377/101‑1 (MQ=255)
                                                                                              gCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAACATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcgg         <  1:3973305/101‑1 (MQ=255)
                                                                                                 gATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc      <  1:250960/101‑1 (MQ=255)
                                                                                                  aTCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCTgcggcgcggcggcgcg     >  1:1548959/1‑101 (MQ=255)
                                                                                                  aTCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCACCccgacgcggcggagcg     >  1:3715506/1‑101 (MQ=21)
                                                                                                    ccGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc   <  1:4064135/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
TTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCG  >  NC_014034/432372‑432571

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG