Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 513,941 Δ1 bp 100% coding (1662/1929 nt) mcpI ← methyl‑accepting chemotaxis protein McpI

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034513,9410A→.100.0% 728.7 163coding (1662/1929 nt)mcpImethyl‑accepting chemotaxis protein McpI
Reads supporting (aligned to +/- strand):  new base (68/94):  ref base (1/0):  total (69/94)

Only 100 of 211 total aligned reads displayed.

GCCGACGGGGGTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAACAAGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGTCACC  >  NC_014034/513841‑514037
                                                                                                    |                                                                                                 
gCCGACGGGGGTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAACa                                                                                                   <  1:3485046/101‑1 (MQ=255)
 ccGACGGGGGTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAACag                                                                                                  <  2:2537575/101‑2 (MQ=255)
     cGGGGGTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCCGCGCGGCGCAGAGGCCCCGGGGTCCGGGCGAAACagaacc                                                                                              >  1:4215155/1‑96 (MQ=255)
      gggggTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCa                                                                                            <  2:1241631/101‑1 (MQ=255)
      gggggTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCa                                                                                            <  1:4636839/101‑1 (MQ=255)
      gggggTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAAGGGTTTGGGGCGGAAC.AGCACCa                                                                                            >  1:3874542/1‑101 (MQ=255)
       ggggTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCAt                                                                                           <  2:3590500/101‑1 (MQ=255)
       ggggTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCAt                                                                                           <  2:208099/101‑1 (MQ=255)
       ggggTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGGGGGGAAC.AGCACCac                                                                                           >  1:1976738/1‑100 (MQ=255)
          gTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGc                                                                                        <  2:3211467/101‑1 (MQ=255)
            ttttggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCt                                                                                      <  2:1068449/101‑1 (MQ=255)
            ttttggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCt                                                                                      <  2:2886580/101‑1 (MQ=255)
             tttggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTc                                                                                     >  2:3710677/1‑101 (MQ=255)
             tttggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTc                                                                                     <  1:3199316/101‑1 (MQ=255)
             tttggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAAGGGGTCGGGGCGGAAC.AGCCCCCTCGcccc                                                                                     >  1:4091703/1‑99 (MQ=255)
              ttggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGGTTTGGGGGGGAAC.AGCACCATCGCCTcc                                                                                    >  1:45517/1‑100 (MQ=255)
              ttggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGGTTCGGTGCGGAAC.AGCACCATCGCCTcc                                                                                    >  1:4294926/1‑100 (MQ=255)
               tggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTcgc                                                                                   <  1:3181847/101‑1 (MQ=255)
                ggttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTcgcg                                                                                  <  1:2405369/101‑1 (MQ=255)
                  ttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCa                                                                                <  1:982013/101‑1 (MQ=255)
                  ttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCa                                                                                <  2:1587986/101‑1 (MQ=255)
                  ttttggGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGGGCGGAA.AAGCACCATCGCCCCgcgcc                                                                                >  1:1853618/1‑100 (MQ=255)
                      gggggCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAAGGCTTCGGTGCGAAA.AAGCAACATCCCCTCgcgccggtg                                                                            >  2:2331089/1‑96 (MQ=255)
                       ggggCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCCTCGGTGCGAAAC.AGCACCATCGCCCCGCGCCGtctc                                                                           >  2:1643849/1‑98 (MQ=255)
                         ggCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.gcgc                                                                        <  2:369142/101‑1 (MQ=255)
                          gCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGcc                                                                       <  2:4610583/101‑1 (MQ=255)
                           cTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGGGCGAAAC.AGCACCATCGCCCCCCGCAGGT.GCGccc                                                                      >  1:926183/1‑100 (MQ=255)
                            tttGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGGTTTGGGGCGAAACAA.CACCCTCGCCTCGCGCAGTT.GCGCCGa                                                                     >  2:3325726/1‑101 (MQ=255)
                             ttGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGAc                                                                    <  2:525208/101‑1 (MQ=255)
                              tGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGGCCCGGCGCGGCGCAGAGGCCAGGGCTTCGGGGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACa                                                                   >  1:4132859/1‑101 (MQ=255)
                              tGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACa                                                                   <  2:309738/101‑1 (MQ=255)
                                 tttCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGGCTCGGTGCGAAA.AAGCAACATCGCCTCGCGCAGTTGGCGCC.ACATGc                                                                >  1:441073/1‑101 (MQ=255)
                                   tCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGccc                                                              <  1:79193/101‑1 (MQ=255)
                                   tCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGGGCGAAAC.AGCACCATCGCCCCGCGCCGTT.GCGCCGACATGccc                                                              >  1:2423405/1‑101 (MQ=255)
                                    cggcggGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCg                                                             <  2:1363080/101‑1 (MQ=255)
                                    cggcggGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAAGGCTTCGGTGCGAAA.AAGCACCATCGCCTCGCGCCGGT.GGGcccaactggccg                                                             >  2:2309962/1‑91 (MQ=255)
                                      gcggGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGAc                                                           <  2:3774798/101‑1 (MQ=255)
                                        ggggCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAg                                                         >  2:3176482/1‑101 (MQ=255)
                                         gggCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGc                                                        <  1:2695641/101‑1 (MQ=255)
                                          ggCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCa                                                       <  1:863743/101‑1 (MQ=255)
                                             ttGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCAccc                                                    <  1:4241909/101‑1 (MQ=255)
                                              tGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCg                                                   <  1:4319239/101‑1 (MQ=255)
                                              tGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCg                                                   <  1:3076692/101‑1 (MQ=255)
                                              tGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCg                                                   <  2:197271/101‑1 (MQ=255)
                                              tGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGGGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCg                                                   >  2:2501146/1‑101 (MQ=255)
                                               gCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCgg                                                  <  2:4465493/101‑1 (MQ=255)
                                                ccGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGc                                                 >  1:3594225/1‑101 (MQ=255)
                                                ccGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGc                                                 <  1:179233/101‑1 (MQ=255)
                                                ccGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCCCCGACATGCCCGACAGCACCCGGc                                                 >  2:2590303/1‑101 (MQ=255)
                                                 cGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCt                                                <  1:3782681/101‑1 (MQ=255)
                                                  gggCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCtt                                               <  2:4132571/101‑1 (MQ=255)
                                                  gggCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCtt                                               <  1:1580420/101‑1 (MQ=255)
                                                   ggCTCAGGCGACCCGGCGCGTCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTg                                              <  2:1528372/101‑1 (MQ=255)
                                                   ggCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGCCATGCCCGACAGCACCCCGCttc                                              >  2:3903787/1‑100 (MQ=255)
                                                   ggCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTg                                              <  1:752047/101‑1 (MQ=255)
                                                    gCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTgc                                             >  1:1504087/1‑101 (MQ=255)
                                                     cTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTgcc                                            <  2:897977/101‑1 (MQ=255)
                                                      tCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTgccg                                           >  1:2800228/1‑101 (MQ=255)
                                                      tCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTgccg                                           <  1:3387115/101‑1 (MQ=255)
                                                        aGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTgccgcc                                         >  1:1370940/1‑101 (MQ=255)
                                                        aGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTgccgcc                                         >  2:3421782/1‑101 (MQ=255)
                                                        aGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTgccgcc                                         <  1:2383357/101‑1 (MQ=255)
                                                        aGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCCCCGACATGCCCGACAGCACCCGGCTTgccgcc                                         <  2:2729546/101‑1 (MQ=255)
                                                        aGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGGGCGGAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACGCGGCTTgccgcc                                         >  2:1864032/1‑101 (MQ=255)
                                                         ggCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGcccccg                                        >  1:2630981/1‑101 (MQ=255)
                                                         gccggcccGGCGCGGCGCAGAGGCCAGGCCTTCGGTGCGAAAC.AGCGCCGTCGCCCGGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGccgccg                                        <  2:2161915/96‑1 (MQ=255)
                                                          gCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCcgt                                       >  2:4649289/1‑101 (MQ=255)
                                                           cGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGccgccgcc                                      <  2:638570/101‑3 (MQ=255)
                                                            gACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCcgtcg                                     <  2:454971/101‑1 (MQ=255)
                                                              cccGGCGCGGCGCAGAGGCCAGGGCTTCTGGGCGAAAC.AGCACCATCGCCTCGCGCAGTG.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGtt                                   <  1:1774934/101‑1 (MQ=255)
                                                                cggcgcggcgCAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGTCAGCACCCGGCTTGCCGCCGTCGTTTc                                 <  1:3095163/101‑1 (MQ=255)
                                                                 ggcgcggcgcAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTcc                                >  1:1237275/1‑101 (MQ=255)
                                                                 ggcgcggcgcAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGCCGTTAcc                                >  2:3929622/1‑101 (MQ=255)
                                                                   cgcggcgcAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTc                              >  2:2466512/1‑101 (MQ=255)
                                                                      ggcgcAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGaa                           <  1:2331251/101‑1 (MQ=255)
                                                                         gcggagGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACAt                        <  1:3004348/98‑1 (MQ=255)
                                                                          cAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATc                       >  2:1181962/1‑101 (MQ=255)
                                                                          cAGAGGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATc                       <  2:2800012/101‑1 (MQ=255)
                                                                          cAGAAGCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCCCGAACATc                       >  2:1733162/1‑101 (MQ=255)
                                                                              ggCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCa                   <  1:2553195/101‑1 (MQ=255)
                                                                               gCCAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCAc                  >  1:4610904/1‑101 (MQ=255)
                                                                                 cAGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGt                <  1:1871912/101‑1 (MQ=255)
                                                                                  aGGGCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGtt               >  1:559979/1‑101 (MQ=255)
                                                                                   ggtcTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTc              >  1:3338018/4‑101 (MQ=255)
                                                                                   gggCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTc              >  1:600586/1‑101 (MQ=255)
                                                                                     gCTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTctg            >  2:711870/1‑101 (MQ=255)
                                                                                      cTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTctgc           <  2:4091417/101‑1 (MQ=255)
                                                                                      cTTCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCCCCGACATGCCCGACAGCACCCGGCTTGCCCCCGTCGTTTTCTCGAACATCGCCACGCTctgc           >  2:1304895/1‑101 (MQ=255)
                                                                                       ttCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTctgct          >  2:1139711/1‑101 (MQ=255)
                                                                                       ttCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTctgct          <  1:2207089/101‑1 (MQ=255)
                                                                                        tCGGTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTctgctg         >  2:2408940/1‑101 (MQ=255)
                                                                                          ggTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCg       >  2:2029724/1‑101 (MQ=255)
                                                                                          ggTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGCCGTTTCCTCTAACACCGCCACGCTCTGCTGCg       >  1:3286527/1‑101 (MQ=255)
                                                                                           gTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGt      <  2:3814785/101‑1 (MQ=255)
                                                                                           gTGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGt      >  2:4301964/1‑101 (MQ=255)
                                                                                            tGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGTc     >  2:2841148/1‑101 (MQ=255)
                                                                                            tGCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGTc     <  1:4264688/101‑1 (MQ=255)
                                                                                             gCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGTCa    >  2:3870636/1‑101 (MQ=255)
                                                                                             gCGAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGTCa    <  2:3487421/101‑1 (MQ=255)
                                                                                               gAAAC.AGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGTCAcc  <  1:1097847/101‑1 (MQ=255)
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GCCGACGGGGGTTTTTGGTTTTGGGGGCTTTGGTTTCGGCGGGGCTTGCCGGGCTCAGGCGACCCGGCGCGGCGCAGAGGCCAGGGCTTCGGTGCGAAACAAGCACCATCGCCTCGCGCAGTT.GCGCCGACATGCCCGACAGCACCCGGCTTGCCGCCGTCGTTTCCTCGAACATCGCCACGTTCTGCTGCGTCACC  >  NC_014034/513841‑514037

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG