Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 735,234 Δ1 bp 2.0% intergenic (‑140/‑345) bchF ← / → ppaA 2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034735,2340C→. 2.0% 4.4 ‑0.5 ‑0.1197intergenic (‑140/‑345)bchF/ppaA2‑vinyl bacteriochlorophyllide hydratase/regulatory protein PpaA
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (105/86):  total (109/88)
Fisher's exact test strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.80e-01

Only 100 of 240 total aligned reads displayed.

AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCG  >  NC_014034/735134‑735333
                                                                                                     |                                                                                                   
aaaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.c                                                                                                     >  1:3627237/1‑101 (MQ=255)
 aaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cc                                                                                                    >  2:2389476/1‑101 (MQ=255)
 aaaCTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cc                                                                                                    <  2:2667060/101‑1 (MQ=255)
    cTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccc                                                                                                 <  1:10416/101‑1 (MQ=255)
     tGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccccc                                                                                                >  1:4400128/1‑101 (MQ=255)
     tGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccccc                                                                                                >  2:1426860/1‑101 (MQ=255)
     tGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccccc                                                                                                <  1:3009396/101‑1 (MQ=255)
      ggTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccccc                                                                                               >  1:61620/1‑101 (MQ=255)
       gTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccccccc                                                                                              >  1:460644/1‑101 (MQ=255)
       gTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.cccccccc                                                                                              <  1:1765819/101‑1 (MQ=255)
        tCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGGACGCAAGGGCGCCTTGATGCAGCGG.ccccccccc                                                                                             >  1:4004494/1‑101 (MQ=255)
        tCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.ccccccccc                                                                                             >  2:910540/1‑101 (MQ=255)
         cTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCg                                                                                            >  1:3134102/1‑101 (MQ=255)
         cTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCg                                                                                            >  1:2724676/1‑101 (MQ=255)
          ttGTCACGAGAAAGTGTTAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.TCACCCCCCgg                                                                                           >  2:664496/1‑101 (MQ=255)
           tGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGc                                                                                          >  1:859599/1‑101 (MQ=255)
           tGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGc                                                                                          >  1:2696773/1‑101 (MQ=255)
           tGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGc                                                                                          >  2:3810824/1‑101 (MQ=255)
            gTCACGAGAAAGTGTCAATGAAAACTTACACCCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCt                                                                                         >  1:4132631/1‑101 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAc                                                                                     >  2:2204410/1‑101 (MQ=255)
                cGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAc                                                                                     >  2:4305784/1‑101 (MQ=255)
                 gagaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAcc                                                                                    <  1:4663625/101‑1 (MQ=255)
                  agaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACcg                                                                                   <  1:3434720/101‑1 (MQ=255)
                  agaAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACcg                                                                                   <  2:3948699/101‑1 (MQ=255)
                     aaGTGTCAATGAAACCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCgg                                                                                <  2:2400868/101‑1 (MQ=255)
                     aaGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCgg                                                                                >  1:1843609/1‑101 (MQ=255)
                        tgtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCa                                                                             >  1:1374872/1‑101 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCAc                                                                            <  2:3434524/101‑1 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCAc                                                                            <  1:4546858/101‑1 (MQ=255)
                         gtCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCAc                                                                            <  2:1527517/101‑1 (MQ=255)
                           taaTTAAAACTTGCGCTCAAGGGGCGGACCAAACCCCGCCTCCCTTGGGCGCAAGGGCTCCTTTGTGCCGGCG.CCCCCCCCCGGCTAAACCGCGGTCACGa                                                                          <  2:3092901/100‑1 (MQ=255)
                           cAATGAAAACTTACCCTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGa                                                                          <  2:892568/101‑1 (MQ=255)
                           cAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGa                                                                          <  2:3173197/101‑1 (MQ=255)
                           cAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGa                                                                          >  1:3506099/1‑101 (MQ=255)
                            aaTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGAc                                                                         <  1:2670719/101‑1 (MQ=255)
                             aTGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACt                                                                        >  1:4111358/1‑101 (MQ=255)
                              tGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTc                                                                       <  2:1373833/101‑1 (MQ=255)
                               gAAAACTTACACGCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCg                                                                      <  1:797875/101‑1 (MQ=255)
                                 aaaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCCGGCTAAACCGCGGTCACGACTCGt                                                                     <  1:1889413/101‑1 (MQ=255)
                                  aaCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTcc                                                                   >  2:1173073/1‑101 (MQ=255)
                                   aCTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGGCCCCCCCCCCGGCTAAACCGCGGTCACGACTCGTcc                                                                   <  1:3069192/101‑1 (MQ=255)
                                    cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCgg                                                                 <  2:1921074/101‑1 (MQ=255)
                                    cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGGCCgg                                                                 >  1:3220333/1‑101 (MQ=255)
                                    cTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAACGGCGCCTTGATCCAGGGG.CTCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCgg                                                                 <  1:3921895/101‑1 (MQ=255)
                                     ttACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCggg                                                                >  1:1111277/1‑101 (MQ=255)
                                       acacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCCCGACTCGTCCGGGCa                                                              >  1:3333279/1‑101 (MQ=255)
                                       acacTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCa                                                              <  2:2140489/101‑1 (MQ=255)
                                         acTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCCCGACCCGCCCGGGCaaa                                                            >  2:2065510/1‑99 (MQ=255)
                                         acTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGa                                                            <  1:1005119/101‑1 (MQ=255)
                                         acTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGa                                                            >  1:210343/1‑101 (MQ=255)
                                          cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           <  2:2268379/101‑1 (MQ=255)
                                          cTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           <  1:1228383/101‑1 (MQ=255)
                                          cTCAGGGGCGGGACGAAACGCCGCTGCCCTGGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaa                                                           <  1:1609065/101‑1 (MQ=255)
                                           tCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGaaa                                                          >  2:4551307/1‑101 (MQ=255)
                                           tCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCACCCCCCGCTAAACCGCGGGCACGACTTGCCCGGGccgcac                                                          >  2:1289531/1‑96 (MQ=255)
                                            cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                         <  1:3170762/101‑1 (MQ=255)
                                            cAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAAc                                                         <  1:1236777/101‑1 (MQ=255)
                                               gggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgg                                                      <  2:3535140/101‑1 (MQ=255)
                                                ggCGGGCCGAACCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcggg                                                     <  2:2338860/101‑1 (MQ=255)
                                                ggCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcggg                                                     <  1:241299/101‑1 (MQ=255)
                                                 gCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggc                                                    <  2:2523232/101‑1 (MQ=255)
                                                   gggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcgg                                                  >  2:472278/1‑101 (MQ=255)
                                                   gggACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACcgggcgg                                                  >  1:4088238/1‑101 (MQ=255)
                                                     gACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCgggcgggg                                                >  2:2031156/1‑101 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                             >  1:3715570/1‑101 (MQ=255)
                                                        gAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGggcggggcgg                                             <  2:4174199/101‑1 (MQ=255)
                                                         aaaCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcggg                                            >  1:1812290/1‑101 (MQ=255)
                                                         aaaCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAAACGCGGACACGACTCGTCCGGGCAAAAACCGGgcggggcggg                                            >  1:1402138/1‑101 (MQ=255)
                                                          ctcgccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGa                                           <  2:3304589/99‑1 (MQ=255)
                                                          aaCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGgcggggcgggg                                           >  1:825104/1‑100 (MQ=255)
                                                           aCGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGACAGAAACCGGGCGGGGCGGGAt                                          >  1:2496600/1‑101 (MQ=255)
                                                            cgccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGaag                                         >  1:3304834/1‑99 (MQ=255)
                                                             gccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCCCGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTgc                                        >  1:1362799/1‑101 (MQ=255)
                                                              ccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgcg                                       <  2:553135/101‑1 (MQ=255)
                                                              ccgcTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATgcg                                       >  2:3183886/1‑101 (MQ=255)
                                                                 cTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGGTGCGCCt                                    >  1:4375624/1‑101 (MQ=255)
                                                                 cTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCt                                    >  2:3636374/1‑101 (MQ=255)
                                                                  cgcccgagcgcccAAGGGCGCCTTGTTGCCGCGG.CACCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCtt                                   <  1:752802/94‑1 (MQ=255)
                                                                   gCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCttt                                  <  1:1527366/101‑1 (MQ=255)
                                                                      cTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTtct                               >  1:2260303/1‑101 (MQ=255)
                                                                      cTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGACGCGCCCTTtcc                               >  2:1626411/1‑100 (MQ=255)
                                                                            cgtaaGGGCGCCATGAGGAAACGG.CCCCCTCCCGGCTAATCCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGCCGGGATGCGCCTTTTCTCAACCt                         <  2:792842/98‑1 (MQ=255)
                                                                            cgcAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCt                         <  1:2169653/101‑1 (MQ=255)
                                                                               aaGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGc                      >  1:3727869/1‑101 (MQ=255)
                                                                                aGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGcc                     <  2:2812630/101‑1 (MQ=255)
                                                                                       cTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCt              <  2:4015254/101‑1 (MQ=255)
                                                                                         tGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTg            >  1:3236771/1‑101 (MQ=255)
                                                                                          gATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATNGGCCCTTTCTCCACCTGGCCCGTATTTTGc           >  1:994858/1‑101 (MQ=255)
                                                                                          gATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGc           >  2:3506620/1‑101 (MQ=255)
                                                                                          gATGCAGCGG.CCCCCCCCCGGCCACACCGCGGGCCCGGCCCGGCCCGGCAGAAACCGGGCCGGGCCGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGc           <  2:497670/101‑1 (MQ=255)
                                                                                            tGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgt         <  2:3719314/101‑1 (MQ=255)
                                                                                              cAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTTcgtcg       >  2:4131723/1‑101 (MQ=255)
                                                                                              cAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcg       <  2:61614/101‑1 (MQ=255)
                                                                                              cAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcg       <  2:3033179/101‑1 (MQ=255)
                                                                                              cAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcgtcg       >  1:1927/1‑101 (MQ=255)
                                                                                               aGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCCACCTGGCCCGTATCTTGcgtcct      >  2:4637686/1‑99 (MQ=255)
                                                                                               aGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGcggcgt      >  2:854274/1‑101 (MQ=255)
                                                                                                  gg.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTgg  >  1:2846525/1‑100 (MQ=255)
                                                                                                  gg.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGc   <  2:2749444/101‑1 (MQ=255)
                                                                                                   g.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCg  <  2:1388842/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
AAAACTGGTCTTGTCACGAGAAAGTGTCAATGAAAACTTACACTCAGGGGCGGGACGAAACGCCGCTGCCCTTGCGCGCAAGGGCGCCTTGATGCAGCGG.CCCCCCCCCGGCTAAACCGCGGTCACGACTCGTCCGGGCAGAAACCGGGCGGGGCGGGATGCGCCTTTTCTCAACCTGGCCCGTATCTTGCGTCGTGGCG  >  NC_014034/735134‑735333

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG