Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014035	34,743	Δ1 bp	100%	coding (399/1041 nt)	RCAP_rcp00049 ←	iron siderophore/cobalamin ABC transporter permease

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014035	34,743	0	G→.	100.0%	128.8	57	coding (399/1041 nt)	RCAP_rcp00049	iron siderophore/cobalamin ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (36/20):  ref base (0/1):  total (36/21)

CGACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGCCAG  >  NC_014035/34643‑34841                                                                                                     |                                                                                                   cgACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATg                                                                                                    <  2:3667427/101‑1 (MQ=32) cgACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATg                                                                                                    <  1:2026045/101‑1 (MQ=32) cgACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATg                                                                                                    >  2:1611918/1‑101 (MQ=32) cgACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATg                                                                                                    >  1:2618911/1‑101 (MQ=32)   ccccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATgga                                                                                                  <  2:4056708/100‑2 (MQ=14)    cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                                 <  2:3873680/101‑3 (MQ=11)    cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                                 <  2:2781426/101‑3 (MQ=11)    cccccGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATggac                                                                                                 <  1:600370/101‑3 (MQ=11)                        ttCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCg                                                                            >  1:4082056/1‑101 (MQ=14)                         tCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGa                                                                           >  2:1729739/1‑101 (MQ=17)                           ggCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATg                                                                         >  1:1251375/1‑101 (MQ=17)                           ggCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATg                                                                         <  1:3088632/101‑1 (MQ=17)                           ggCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCAAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATg                                                                         <  1:2790207/101‑1 (MQ=12)                            gCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGc                                                                        <  2:1804081/101‑1 (MQ=17)                             cccGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGcc                                                                       >  2:943939/1‑101 (MQ=17)                                gCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAg                                                                    >  1:2685575/1‑101 (MQ=17)                                  tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCa                                                                  >  2:3591413/1‑101 (MQ=17)                                  tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCa                                                                  >  2:1716062/1‑101 (MQ=17)                                  tGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGACCAGCa                                                                  >  2:4524893/1‑101 (MQ=12)                                   ggTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCAc                                                                 >  2:2508046/1‑101 (MQ=17)                                      cgagcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGAt                                                              >  2:3753664/4‑101 (MQ=12)                                       gcgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATg                                                             <  2:4269693/101‑1 (MQ=17)                                       gcgcCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATg                                                             >  2:2232122/1‑101 (MQ=17)                                          ccGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAcc                                                          <  2:102877/101‑1 (MQ=17)                                          ccGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAcc                                                          <  2:3324980/101‑1 (MQ=17)                                           cGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGAccc                                                         >  1:546351/1‑100 (MQ=12)                                           cGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCg                                                         >  2:3245781/1‑101 (MQ=18)                                           cGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCg                                                         >  2:3369440/1‑101 (MQ=18)                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                        >  1:1617112/1‑101 (MQ=18)                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                        >  2:488194/1‑101 (MQ=18)                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                        >  2:3242934/1‑101 (MQ=18)                                            gTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGc                                                        <  1:2458064/101‑1 (MQ=17)                                             ttCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGcc                                                       >  2:702662/1‑101 (MQ=18)                                              tCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCa                                                      >  2:386141/1‑101 (MQ=18)                                               cGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAc                                                     >  2:3645253/1‑101 (MQ=18)                                               cGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAc                                                     >  1:13706/1‑101 (MQ=18)                                               cGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAc                                                     <  2:1980806/101‑1 (MQ=17)                                                gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                    >  2:3739763/1‑101 (MQ=18)                                                gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                    <  1:2515500/101‑1 (MQ=17)                                                gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                    <  2:2685379/101‑1 (MQ=17)                                                gACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCAcc                                                    <  2:2533387/101‑1 (MQ=17)                                                   aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgcg                                                 <  1:1364367/101‑1 (MQ=17)                                                   aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgcg                                                 >  1:2810563/1‑101 (MQ=18)                                                   aaaaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACcgcg                                                 <  1:3579265/101‑1 (MQ=17)                                                     aaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGcc                                               >  2:1627377/1‑101 (MQ=18)                                                     aaGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGcc                                               >  1:4203042/1‑101 (MQ=17)                                                        cgcCACGAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGt                                            <  2:2821299/101‑1 (MQ=12)                                                        cgcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGt                                            <  2:4166797/101‑1 (MQ=17)                                                         gcCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTc                                           <  1:3178021/101‑1 (MQ=17)                                                          ccaccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCg                                          <  1:1138625/101‑1 (MQ=17)                                                             ccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAgg                                       <  2:2426874/101‑1 (MQ=17)                                                             ccaGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAgg                                       >  1:1480137/1‑101 (MQ=17)                                                              caGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGc                                      >  2:517385/1‑101 (MQ=17)                                                              caGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGc                                      <  2:1790384/101‑1 (MQ=17)                                                               aGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGcc                                     >  1:3102667/1‑101 (MQ=17)                                                               aGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGcc                                     >  1:690481/1‑101 (MQ=17)                                                                  gcgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCA.GCGACACCGACAGCCCGCCCGGCCGTTGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGccccc                                  <  1:705523/101‑1 (MQ=11)                                                                   cgAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccg                                 >  1:199572/1‑101 (MQ=17)                                                                     aaGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGG.CAGGGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgcc                               >  2:2052664/1‑101 (MQ=255)                                                                     aaGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgcc                               >  1:2285284/1‑101 (MQ=17)                                                                      aGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgccg                              >  1:3668460/1‑101 (MQ=17)                                                                      aGGCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCccgccg                              <  2:3225250/101‑1 (MQ=17)                                                                       ggCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCga                             >  1:3328282/1‑101 (MQ=17)                                                                       ggCAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCga                             >  1:1641669/1‑101 (MQ=17)                                                                         cAGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCgaga                           >  2:3771850/1‑101 (MQ=14)                                                                          aGCGAAGGCGCCCGCGAAAGCCCCCAT.GGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGAc                          >  1:723467/1‑101 (MQ=11)                                                                               aGGCGCCCGCGAAAGCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCa                     <  2:2751033/101‑1 (MQ=17)                                                                                         gAACGCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATCCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAggg           <  2:3928395/101‑1 (MQ=255)                                                                                           aaGCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCCCCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGtt         <  1:3753939/101‑1 (MQ=12)                                                                                            aGCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTc        <  2:3649144/101‑1 (MQ=17)                                                                                             gCCCCCA.GGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCg       <  1:3246026/101‑1 (MQ=17)                                                                                                ccccagggACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGCGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGc     <  2:2013024/96‑1 (MQ=12)                                                                                                   cagggACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGCCAg  <  1:720572/99‑1 (MQ=25)                                                                                                     |                                                                                                   CGACCCCCGCGAGGATCGTGTGGTTCGGCCCGCTGGTCGCGCCGTTCGACAAAAGCGCCACCAGCGCGAAGGCAGCGAAGGCGCCCGCGAAAGCCCCCATGGGACAGCGACAGCCCGCCCGGCCGATGCCCAGCACGATGACCGCCACCGCGCCGGTCGAGGCCCCCGCCGAGACGCCCAGCACGAAGGGTTCGGCCAG  >  NC_014035/34643‑34841 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG