Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,200,790 +G 2.2% coding (671/672 nt) trpF → phosphoribosylanthranilate isomerase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,200,7902.→G 2.2% 5.1 ‑4.2 ‑0.1181coding (671/672 nt)trpFphosphoribosylanthranilate isomerase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (60/117):  total (64/117)
Fisher's exact test strand distribution p-value = 1.47e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.49e-01

Only 100 of 220 total aligned reads displayed.

ACCGGGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200690‑1200889
                                                                                                     |                                                                                                       
gccGGGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTa                                                                                                          <  2:3524032/100‑1 (MQ=255)
aCCGGGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTa                                                                                                          <  1:3430640/101‑1 (MQ=255)
 ccGGGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCCGGGGGA...g                                                                                                      >  1:2147093/1‑101 (MQ=255)
  cGGGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...gg                                                                                                     <  1:332158/101‑1 (MQ=255)
  cGGGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...gg                                                                                                     <  2:1194353/101‑1 (MQ=255)
   ggggCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...ggg                                                                                                    <  2:2258986/101‑1 (MQ=255)
   ggggCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...ggg                                                                                                    <  2:1607547/101‑1 (MQ=255)
    gggCGGCTCAGGTCGACGTTTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGt                                                                                                   <  1:1321594/101‑1 (MQ=255)
    gggCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...gggg                                                                                                   >  2:1520464/1‑100 (MQ=255)
     ggcggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.g                                                                                                 <  2:4300109/101‑1 (MQ=255)
      gcggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gg                                                                                                <  2:36515/101‑1 (MQ=255)
        ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gggg                                                                                              <  2:1066167/101‑1 (MQ=255)
        ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gggg                                                                                              <  1:1339648/101‑1 (MQ=255)
         gcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggg                                                                                             >  2:4330372/1‑100 (MQ=255)
         gcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA...GGGG.ggggg                                                                                             >  2:780623/1‑100 (MQ=255)
          cTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.gggggg                                                                                            >  2:3640969/1‑99 (MQ=255)
            cAGGTGGGCGTGTCGTCGGGGGTGGACTCGGCGCCCGGGGTTAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.ttt                                                                                         <  1:1632179/101‑2 (MQ=255)
            cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.ttt                                                                                         <  1:3013587/101‑2 (MQ=255)
            cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggggtg                                                                                          >  1:199469/1‑97 (MQ=255)
             agtgcGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.tttc                                                                                        <  2:3934303/97‑3 (MQ=255)
             aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.ggggggttc                                                                                         >  2:1458496/1‑96 (MQ=255)
             aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.tttc                                                                                        <  2:1797244/101‑3 (MQ=255)
             aGGTCGACGGGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.tttc                                                                                        <  1:1250513/101‑3 (MQ=255)
               gTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggggttcac                                                                                       >  2:1880234/1‑94 (MQ=255)
                tCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTcacc                                                                                      <  1:2120307/101‑1 (MQ=255)
                 cGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTcaccc                                                                                     >  1:3584183/1‑101 (MQ=255)
                 cGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggggtttaccc                                                                                     >  1:2458050/1‑92 (MQ=255)
                   aCGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTcaccccc                                                                                   <  1:1520573/101‑1 (MQ=255)
                     gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTcacccccac                                                                                 <  2:4428216/101‑1 (MQ=255)
                     gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTcacccccac                                                                                 <  2:2961417/101‑1 (MQ=255)
                     gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGCA...GGGTGGGGGT.TTcacccccac                                                                                 >  1:3846186/1‑101 (MQ=255)
                       gtcgtcGGGGGGGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTcaccccccccc                                                                               >  1:1460518/1‑97 (MQ=255)
                          gtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTtaccccccaacccc                                                                            >  2:1037224/1‑101 (MQ=255)
                          gtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTcacccccccacccc                                                                            >  2:2297958/1‑101 (MQ=255)
                            cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCCGGGGTAG..GGGG.GGGGG.TTcacccccaacccccca                                                                          >  2:2095230/1‑101 (MQ=255)
                            cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTcacccccacacccccc                                                                          >  1:4461096/1‑100 (MQ=255)
                            cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTcacccccacaccccca                                                                          >  1:2913430/1‑101 (MQ=255)
                            cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTcacccccacacccccc                                                                          >  2:2766540/1‑100 (MQ=255)
                             gggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAg                                                                         <  1:830605/101‑1 (MQ=255)
                              ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCCCCCCCACACCCCCAGc                                                                        <  1:383440/101‑1 (MQ=255)
                                ggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGt                                                                      <  2:4091405/101‑1 (MQ=255)
                                 gTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTa                                                                     <  2:3204655/101‑1 (MQ=255)
                                    gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTAttt                                                                  <  2:1234507/101‑1 (MQ=255)
                                    gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTAttt                                                                  <  1:3392084/101‑1 (MQ=255)
                                     aaTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGG.GGGG.GGGGT.TTaacccccccccccccgccgatttt                                                                  >  2:44548/1‑92 (MQ=255)
                                       tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTgg                                                               <  1:2200259/101‑1 (MQ=255)
                                        cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTCACCCCCACACCCCCGGCGGATTTTGGc                                                              >  2:2238529/1‑101 (MQ=255)
                                        cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGc                                                              <  1:1854608/101‑1 (MQ=255)
                                         ggCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCCCCCCCACACCCCCAGCGTATTTTGGcc                                                             <  1:4401559/101‑1 (MQ=255)
                                         ggCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGcc                                                             <  1:304339/101‑1 (MQ=255)
                                             cccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGGGG.GGGGT.TTCCCCCCCACCCCCCCCGCATTTTTTgccaca                                                           >  2:3196805/1‑96 (MQ=255)
                                               cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTCACCCCCCCACCCCCAGCGTATTTTGGCCaagaag                                                       >  1:3773724/1‑101 (MQ=255)
                                                 gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAgaagaa                                                     <  2:33651/101‑1 (MQ=255)
                                                 gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAgaagaa                                                     <  1:3711850/101‑1 (MQ=255)
                                                 gggTCAAGGAGGCCGCCCCGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAgaagaa                                                     <  1:4643868/101‑1 (MQ=255)
                                                  ggTCAGGGAGGCCGCCCGGATTGCCGCCTGTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGaaa                                                    <  2:2707492/101‑1 (MQ=255)
                                                  ggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCCCACCCCCCGCGTATTTTGGCCAAGAAGaaa                                                    >  1:2740145/1‑101 (MQ=255)
                                                     cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAg                                                 <  2:3617849/101‑1 (MQ=255)
                                                     cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTTACCCCCACACCCCCAGCGTATTTTGGCACAGAAGAAAAAg                                                 >  2:761289/1‑101 (MQ=255)
                                                      aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTCACCCCCACACCCCCCGCGGATTTTGGCCAAGAAGAAAAAgg                                                >  1:3347596/1‑101 (MQ=255)
                                                       aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTTAACCCCACACCCCCCCCGCATTTTGGCCAAGGAAAAACATGa                                               >  1:3626698/1‑101 (MQ=255)
                                                       aggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTCACCCCCACACCCCCAGCGGATTTTGGCCAAGAAGAAACAGGa                                               >  1:4341487/1‑101 (MQ=255)
                                                        ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTTACCCCCACACCCCCACGGTATTTTTGCCAAGAAGAAACaagca                                              >  1:4384528/1‑97 (MQ=255)
                                                        ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGaa                                              <  1:986794/101‑1 (MQ=255)
                                                        ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGaa                                              <  1:560817/101‑1 (MQ=255)
                                                        ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGaa                                              <  2:3058109/101‑1 (MQ=255)
                                                         gaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAAc                                             <  2:957606/101‑1 (MQ=255)
                                                          aggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACt                                            <  1:2633549/101‑1 (MQ=255)
                                                          aggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACt                                            <  1:3781464/101‑1 (MQ=255)
                                                            gccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCTCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACttt                                          <  2:2384859/101‑1 (MQ=255)
                                                             ccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTa                                         <  1:3093168/101‑1 (MQ=255)
                                                              cgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGATGAAACAGGAACTTTAc                                        <  1:4074621/101‑1 (MQ=255)
                                                              cgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAc                                        <  1:4511524/101‑1 (MQ=255)
                                                               gccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAcc                                       <  2:3988762/101‑1 (MQ=255)
                                                                cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAccc                                      <  2:1815760/101‑1 (MQ=255)
                                                                cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAccc                                      >  2:373685/1‑101 (MQ=255)
                                                                 ccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAcccc                                     <  1:944901/101‑1 (MQ=255)
                                                                 ccGGAGTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTAcccc                                     <  1:2301345/101‑1 (MQ=255)
                                                                  cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...TGGTGGGGGG.TTCACCCCCACACCCCCAGCGGATTTTGGCCAAGAAGAAACAGGAACTTTACCCCg                                    >  1:2705342/1‑101 (MQ=255)
                                                                  cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCg                                    <  2:2149702/101‑1 (MQ=255)
                                                                   ggATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCgg                                   >  2:2539061/1‑100 (MQ=255)
                                                                    gATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAg                                  <  2:2909972/101‑1 (MQ=255)
                                                                     aTTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAgg                                 <  1:3230863/101‑1 (MQ=255)
                                                                      ttGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAggg                                <  2:1493301/101‑1 (MQ=255)
                                                                            ccTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgc                          <  2:718153/101‑1 (MQ=255)
                                                                             cTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTCACCCCCACACACCCAGCGTATTTTGGCCAAGAAGAAACAGGCACTTTACCCCGAGGGGGGGgcg                         >  2:793464/1‑101 (MQ=255)
                                                                              tttGTCGCGGCGGCGCAGGGGTAGGGGGGG.GGGGTGTTCCCCCCCCCCCCCCCCG.GCTTTTTGCCCAAAAAAAAAAAGAAACTTTCCCCCCGggggggggg                          >  1:1742564/1‑100 (MQ=255)
                                                                                    gcggcggcgCAGGGGTA...GGGTGGGGGT.TTCCCCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATc                  <  2:3187691/101‑1 (MQ=255)
                                                                                    gcggcggcgCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGGGCTATc                  >  2:3440226/1‑101 (MQ=255)
                                                                                      ggcggcgCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg                <  1:4283440/101‑1 (MQ=255)
                                                                                        cggcgCAGGGTTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctgct              <  2:3312269/101‑1 (MQ=255)
                                                                                           cgcAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGtt           <  2:3806320/101‑1 (MQ=255)
                                                                                            gcAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTg          >  2:422694/1‑101 (MQ=255)
                                                                                            gcAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTg          <  2:358545/101‑1 (MQ=255)
                                                                                             cAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTgg         <  1:2539235/101‑1 (MQ=255)
                                                                                              aGGGGTA...GGGTGGGGGG.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTggg        >  2:2819680/1‑100 (MQ=255)
                                                                                                   tA...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTg   >  1:586918/1‑101 (MQ=255)
                                                                                                    a...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  >  2:643411/1‑101 (MQ=255)
                                                                                                    a...GGGTGGGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  <  1:1152559/101‑1 (MQ=255)
                                                                                                    a...GGGTGGGGGG.TTCACCCCCACACCCCCCGCGTATTTTTGGCAAGAAGAAAAAGGAACTTTACCCCGAGGGGGGGGGGCGCTCTCTGCTGTTGGGGCGGGa  >  2:3301659/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
ACCGGGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGT.TTCACCCCCACACCCCCAGCGTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200690‑1200889

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG