Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,200,794 +G 100% intergenic (+3/‑114) trpF → / → trpB1 phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,200,7941.→G100.0% 99.6 162intergenic (+3/‑114)trpF/trpB1phosphoribosylanthranilate isomerase/tryptophan synthase subunit beta
Reads supporting (aligned to +/- strand):  new base (32/99):  ref base (22/9):  total (54/108)
Fisher's exact test strand distribution p-value = 2.60e-06
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.45e-03

Only 100 of 204 total aligned reads displayed.

GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200694‑1200889
                                                                                                        |                                                                                                 
gggCGGCTCAGGTCGACGTTTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGt                                                                                                    <  1:1321594/101‑1 (MQ=255)
  gcggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gg                                                                                                 <  2:36515/101‑1 (MQ=255)
    ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gggg                                                                                               <  1:1339648/101‑1 (MQ=255)
    ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gggg                                                                                               <  2:1066167/101‑1 (MQ=255)
    ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gggg                                                                                               <  1:2090416/101‑1 (MQ=255)
    ggcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gggg                                                                                               <  1:855194/101‑1 (MQ=255)
     gcTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGGA...GGGG.ggggg                                                                                              >  2:780623/1‑100 (MQ=255)
      cTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.gggggt                                                                                             <  2:4436939/101‑3 (MQ=255)
      cTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.gggggg                                                                                             >  2:3640969/1‑99 (MQ=255)
       tCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.tt                                                                                           <  1:3106302/101‑1 (MQ=255)
       tCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggggt                                                                                            >  1:2126147/1‑98 (MQ=255)
        cAGGTGGGCGTGTCGTCGGGGGTGGACTCGGCGCCCGGGGTTAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.ttt                                                                                          <  1:1632179/101‑2 (MQ=255)
        cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.ttt                                                                                          <  1:4396326/101‑2 (MQ=255)
        cAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggggtg                                                                                           >  1:199469/1‑97 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.ggggggttc                                                                                          >  2:1458496/1‑96 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.tttc                                                                                         <  2:1797244/101‑3 (MQ=255)
         aGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggggttc                                                                                          >  2:2919674/1‑96 (MQ=255)
         aGGTCGACGGGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGg.tttc                                                                                         <  1:1250513/101‑3 (MQ=255)
           gTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.ggggggttcac                                                                                        >  2:1880234/1‑94 (MQ=255)
           gTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGG.gggggggggtg                                                                                        >  1:3312518/1‑94 (MQ=255)
                 gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTcacccccac                                                                                  <  2:2961417/101‑1 (MQ=255)
                 gtgtCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGCA...GGGTGGGGGT.TTcacccccac                                                                                  >  1:3846186/1‑101 (MQ=255)
                   gtcgtcGGGGGGGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTcaccccccccc                                                                                >  1:1460518/1‑97 (MQ=255)
                     cgtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGT.TTcgcccccacaacc                                                                              >  2:1152467/1‑101 (MQ=255)
                      gtcgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTcacccccccacccc                                                                             >  2:2297958/1‑101 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCCGGGGTAG..GGGG.GGGGG.TTcacccccaacccccca                                                                           >  2:2095230/1‑101 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTcacccccacacccccc                                                                           >  1:4461096/1‑100 (MQ=255)
                        cgGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTcacccccacaccccca                                                                           >  1:2913430/1‑101 (MQ=255)
                         gggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAg                                                                          <  2:2196993/101‑1 (MQ=255)
                          ggggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCCCCCCCACACCCCCAGc                                                                         <  1:383440/101‑1 (MQ=255)
                           gggTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCCGGGGTAG..GGGG.GGGGG.TTTACCCCCCCACCCCCAGC.g                                                                       >  1:4041446/1‑101 (MQ=255)
                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTAttt                                                                  <  1:3392084/101‑1 (MQ=255)
                                gAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTAttt                                                                  <  2:1234507/101‑1 (MQ=255)
                                 aaTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTAtttt                                                                 <  2:2783948/101‑1 (MQ=255)
                                  aTCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCCCACCCCCAGC.GTATTTTg                                                                >  1:358582/1‑101 (MQ=255)
                                   tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTgg                                                               <  1:2200259/101‑1 (MQ=255)
                                   tCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTgg                                                               <  1:4429866/101‑1 (MQ=255)
                                    cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTCACCCCCACACCCCCAGC.GTATTTTGGc                                                              >  1:1519582/1‑101 (MQ=255)
                                    cGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGc                                                              <  1:1854608/101‑1 (MQ=255)
                                        gcCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTCACCCCCACACCCCCAGC.GTATTTTGGCCaag                                                          >  2:2524294/1‑101 (MQ=255)
                                         cccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGGGGGGG.GGGGT.TTCCCCCCCACCCCCCCCGC.ATTTTTTgccaca                                                           >  2:3196805/1‑96 (MQ=255)
                                          ccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTCACCCCCCCACCCCCCGC.GCATTTTGGCCcagaa                                                        >  2:3599594/1‑101 (MQ=255)
                                          ccGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCCCCCCCACACCCCCAGC.GTATTTTGGCCaagaa                                                        <  1:3075692/101‑1 (MQ=255)
                                           cGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTCACCCCCCCACCCCCAGC.GTATTTTGGCCaagaag                                                       >  1:3773724/1‑101 (MQ=255)
                                             gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTCACCCCCACACCCCCCGC.GTATTTTTGCCAAgaagaa                                                     >  1:4361083/1‑101 (MQ=255)
                                             gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAgaagaa                                                     <  1:3711850/101‑1 (MQ=255)
                                             gggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAgaagaa                                                     <  2:33651/101‑1 (MQ=255)
                                             gggTCAAGGAGGCCGCCCCGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAgaagaa                                                     <  1:4643868/101‑1 (MQ=255)
                                              ggTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCCCACCCCCCGC.GTATTTTGGCCAAGAAGaaa                                                    >  1:2740145/1‑101 (MQ=255)
                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAg                                                 <  2:2849410/101‑1 (MQ=255)
                                                 cAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTTACCCCCACACCCCCAGC.GTATTTTGGCACAGAAGAAAAAg                                                 >  2:761289/1‑101 (MQ=255)
                                                  aaGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TTCACCCCCACACCCCCCGC.GGATTTTGGCCAAGAAGAAAAAgg                                                >  1:3347596/1‑101 (MQ=255)
                                                    ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGaa                                              <  1:560817/101‑1 (MQ=255)
                                                    ggaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGaa                                              <  2:3058109/101‑1 (MQ=255)
                                                     gaggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAAc                                             <  2:957606/101‑1 (MQ=255)
                                                      aggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAGG.GGGG.GGGGG.TTTCCCCCCCCACCCCCCCC.GGATTTTTGCCAAAAAAaaaaaagaca                                             >  2:3985336/1‑94 (MQ=255)
                                                      aggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACt                                            <  1:3024280/101‑1 (MQ=255)
                                                      aggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACt                                            <  1:3781464/101‑1 (MQ=255)
                                                      aggCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACt                                            <  1:2633549/101‑1 (MQ=255)
                                                        gccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCTCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACttt                                          <  2:2384859/101‑1 (MQ=255)
                                                         ccgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTa                                         <  2:3582738/101‑1 (MQ=255)
                                                          cgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTAc                                        <  1:4511524/101‑1 (MQ=255)
                                                          cgccCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTAc                                        <  2:2739947/101‑1 (MQ=255)
                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTAccc                                      <  2:1815760/101‑1 (MQ=255)
                                                            cccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTAccc                                      >  2:373685/1‑101 (MQ=255)
                                                             ccGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTAcccc                                     <  2:3243069/101‑1 (MQ=255)
                                                             ccGGAGTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTAcccc                                     <  1:2301345/101‑1 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTAG..GGGG.GGGGG.TGTACCCCCCCACCCCCCCCGGGTTTTTGGCCAGAAAGAAAAGGAAAATTTCcccc                                     >  2:4028333/1‑101 (MQ=255)
                                                              cGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...TGGTGGGGGG.TTCACCCCCACACCCCCAGC.GGATTTTGGCCAAGAAGAAACAGGAACTTTACCCCg                                    >  1:2705342/1‑101 (MQ=255)
                                                                 aTTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAgg                                 <  2:2706916/101‑1 (MQ=255)
                                                                 aTTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTCACCCCCACAACCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACCTTACCCCGAgg                                 >  2:4324041/1‑101 (MQ=255)
                                                                  ttGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAggg                                <  2:1493301/101‑1 (MQ=255)
                                                                        ccTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCCCCCCCACCCCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgc                          <  2:2146943/101‑1 (MQ=255)
                                                                        ccTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgc                          <  2:718153/101‑1 (MQ=255)
                                                                        ccTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgc                          <  2:2241374/101‑1 (MQ=255)
                                                                        ccTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgc                          <  1:3641237/101‑1 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgcg                         <  1:4330664/101‑1 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgcg                         <  1:2991909/101‑1 (MQ=255)
                                                                         cTTTGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGG.TTCACCCCCACACACCCAGC.GTATTTTGGCCAAGAAGAAACAGGCACTTTACCCCGAGGGGGGGgcg                         >  2:793464/1‑101 (MQ=255)
                                                                          tttGTCGCGGCGGCGCAGGGGTAGGGGGGG.GGGGTGTTCCCCCCCCCCCCCCCCG..GCTTTTTGCCCAAAAAAAAAAAGAAACTTTCCCCCCGggggggggg                          >  1:1742564/1‑100 (MQ=255)
                                                                           tggTCGCGGCGGGGCGGGGGTA...GGGTGGGGGT.TTCGCCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTCCCCCGGGGGGGGGgcgcg                       <  2:100177/99‑1 (MQ=255)
                                                                            tGTCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACGCCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGgcgcgc                      <  1:3501360/101‑1 (MQ=255)
                                                                              tCGCGGCGGCGCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGCGGCGCGCTa                    <  2:3463330/101‑1 (MQ=255)
                                                                                gcggcggcgCAGGGGTA...GGGTGGGGGT.TTCCCCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATc                  <  2:3187691/101‑1 (MQ=255)
                                                                                 cggcggcgCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGGGCTATct                 >  2:380418/1‑101 (MQ=255)
                                                                                 cggcggcgCAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATct                 <  1:735261/101‑1 (MQ=255)
                                                                                  gtcggcgCAGGGGTA...GGGTGGGGGT.TTCCCCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg                <  2:3820038/99‑1 (MQ=255)
                                                                                  ggcggcgCAGGGGTA...GGGTGGGGGG.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctg                >  1:4275450/1‑101 (MQ=255)
                                                                                    cggcgCAGGGTTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATctgct              <  2:3312269/101‑1 (MQ=255)
                                                                                      gcgcAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGAGCTATCTGCTGt            <  1:2524468/101‑1 (MQ=255)
                                                                                       cgcAGGGGTA...GGGTGGGGGT.TTCCCCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGtt           <  1:948931/101‑1 (MQ=255)
                                                                                        gcAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTg          >  2:422694/1‑101 (MQ=255)
                                                                                        gcAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTg          <  2:4384236/101‑1 (MQ=255)
                                                                                        gcAGGGGTA...GGGTGGGGGG.TTCACCCCCCCACCCCCCGC.GGATTTTTGCCAAGAAGAAAAAGGAACCTTACCCCCAGGGGGGGGGGCGCCATCTGCTGGTg          >  2:4271266/1‑101 (MQ=255)
                                                                                         cAGGGGTA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTgg         <  1:2539235/101‑1 (MQ=255)
                                                                                               tA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTg   >  1:586918/1‑101 (MQ=255)
                                                                                               tA...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTg   >  2:1551986/1‑101 (MQ=255)
                                                                                                a...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  >  2:643411/1‑101 (MQ=255)
                                                                                                a...GGGTGGGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGa  <  1:1152559/101‑1 (MQ=255)
                                                                                                a...GGGTGGGGGG.TTCACCCCCACACCCCCCGC.GTATTTTTGGCAAGAAGAAAAAGGAACTTTACCCCGAGGGGGGGGGGCGCTCTCTGCTGTTGGGGCGGGa  >  2:3301659/1‑101 (MQ=255)
                                                                                                        |                                                                                                 
GGGCGGCTCAGGTCGACGTGTCGTCGGGGGTGGAATCGGCGCCCGGGGTCAAGGAGGCCGCCCGGATTGCCGCCTTTGTCGCGGCGGCGCAGGGGTA...GGGT.GGGGT.TTCACCCCCACACCCCCAGC.GTATTTTGGCCAAGAAGAAACAGGAACTTTACCCCGAGGGGGGGGCGCGCTATCTGCTGTTGGCGCGTGA  >  NC_014034/1200694‑1200889

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG