Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,301,060 +C 91.6% intergenic (+17/‑92) urtA → / → urtC urea ABC transporter permease UrtA/urea ABC transporter permease UrtC

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,301,0601.→C91.6% 28.8 ‑1.6 ‑0.8131intergenic (+17/‑92)urtA/urtCurea ABC transporter permease UrtA/urea ABC transporter permease UrtC
Reads supporting (aligned to +/- strand):  new base (50/70):  ref base (2/9):  total (52/79)
Fisher's exact test strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.62e-01

Only 100 of 143 total aligned reads displayed.

TGGATGATCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGG.CG.C.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTCCCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGA  >  NC_014034/1300960‑1301152
                                                                                                      |                                                                                             
tGGATGATCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGc.cg                                                                                                >  2:2204633/1‑101 (MQ=255)
 ggATGATCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGc.cg.c                                                                                              >  1:3454962/1‑101 (MQ=255)
 ggATGATCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGc.cg.c                                                                                              >  1:4184341/1‑101 (MQ=255)
  gatgatCTGCTTCATCATCATCTTCGTCCAGTTCCGCCCGCGCGGCATCATGGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.c.c                                                                                            <  2:1945630/101‑1 (MQ=255)
   atgatCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.c.cc                                                                                           >  1:4140509/1‑100 (MQ=255)
    tgatCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.c.cct                                                                                          >  1:3624434/1‑99 (MQ=255)
    tgatCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.c.cct                                                                                          >  2:1655790/1‑99 (MQ=255)
     gatCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.C.Cctt                                                                                         <  1:4307829/101‑1 (MQ=255)
     gatCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.C.Cctt                                                                                         <  2:3518165/101‑1 (MQ=255)
       tCCTCTTCATCATCATCTTCCTCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCGGGAGATTGAGCCGATGCCCCCCGC.CG.C.Cctttc                                                                                       <  2:3004927/101‑3 (MQ=255)
       tCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.C.Cctttc                                                                                       <  2:3283626/101‑3 (MQ=255)
       tCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.C.Cctttc                                                                                       <  2:3976827/101‑3 (MQ=255)
       tCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.C.Cctttc                                                                                       >  2:3580252/1‑99 (MQ=255)
        ccTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCa                                                                                       <  2:3847408/101‑1 (MQ=255)
        ccTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGAGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCa                                                                                       <  1:4493741/101‑1 (MQ=255)
         ctctTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCAt                                                                                      >  1:2720573/1‑101 (MQ=255)
         ctctTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCAt                                                                                      <  2:1242468/101‑1 (MQ=255)
         ctctTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CG.C.Cctttcct                                                                                     >  1:2839981/1‑97 (MQ=255)
           ctTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCt                                                                                    <  1:2770598/101‑1 (MQ=255)
           ctTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCt                                                                                    >  1:3142521/1‑101 (MQ=255)
            ttCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCtt                                                                                   <  1:3884671/101‑1 (MQ=255)
            ttCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCtt                                                                                   <  2:1353082/101‑1 (MQ=255)
             tcatcatcaTCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTg                                                                                  <  1:3909859/101‑1 (MQ=255)
              catcaccatCAACGTCCCGTTCCGCCCGCGAGGCATCTTCGCGCGGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGc                                                                                 <  1:990851/101‑1 (MQ=255)
                tcatcatcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGccc                                                                               <  1:1650906/101‑1 (MQ=255)
                 catcatcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCa                                                                              <  2:2961994/101‑1 (MQ=255)
                 catcatcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCa                                                                              <  1:839722/101‑1 (MQ=255)
                  atcatcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCaa                                                                             <  2:3442333/101‑1 (MQ=255)
                  atcatcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCaa                                                                             >  1:4420831/1‑101 (MQ=255)
                  atcatcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCaa                                                                             <  1:1795431/101‑1 (MQ=255)
                   tcatcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCaaa                                                                            >  2:1490957/1‑101 (MQ=255)
                    catcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCaaaa                                                                           <  2:3632858/101‑1 (MQ=255)
                    catcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCaaaa                                                                           <  2:1462556/101‑1 (MQ=255)
                     atcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGCCCC.C.CTTTCATCTTGCCCAAAat                                                                          <  1:2645944/101‑1 (MQ=255)
                     atcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAat                                                                          <  2:2649163/101‑1 (MQ=255)
                      tcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAata                                                                         <  1:4085439/101‑1 (MQ=255)
                      tcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAata                                                                         <  1:3307485/101‑1 (MQ=255)
                      tcTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAata                                                                         <  1:2441623/101‑1 (MQ=255)
                       cTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAatat                                                                        <  1:3258992/101‑1 (MQ=255)
                       cTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAatat                                                                        <  1:2810551/101‑1 (MQ=255)
                       cTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAatat                                                                        >  2:1790899/1‑101 (MQ=255)
                          cATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCGGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATccc                                                                     <  2:1792463/101‑1 (MQ=255)
                          cATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATccc                                                                     <  2:4563517/101‑1 (MQ=255)
                          cATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATccc                                                                     <  2:1241106/101‑1 (MQ=255)
                          cATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATccc                                                                     >  2:4163128/1‑101 (MQ=255)
                          cATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATccc                                                                     <  1:2302244/101‑1 (MQ=255)
                           aTCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCcg                                                                    <  1:950019/101‑1 (MQ=255)
                           aTCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCcg                                                                    >  2:1467991/1‑101 (MQ=255)
                           aTCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCcg                                                                    <  1:4012789/101‑1 (MQ=255)
                           aTCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCcg                                                                    <  2:2360401/101‑1 (MQ=255)
                              cAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCgg                                                                 <  1:3103142/101‑1 (MQ=255)
                               aGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCggg                                                                >  1:1675716/1‑101 (MQ=255)
                               aGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCggg                                                                <  2:2503168/101‑1 (MQ=255)
                                gTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCgggg                                                               >  1:155595/1‑101 (MQ=255)
                                  tCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGt                                                             <  2:797159/101‑1 (MQ=255)
                                    cgcccgcGCGGCATCCTGGCGCTGCGCGGCCCCGCGGCCGGAGATTGAGCCGATGCCCCCCGC.CG.CTCTTTCATCTTGCCCAAAATATCCCGCGGGAGTcc                                                           <  1:3430879/101‑1 (MQ=255)
                                    cgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTcc                                                           <  2:3293705/101‑1 (MQ=255)
                                    cgcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTcc                                                           <  2:2771666/101‑1 (MQ=255)
                                     gcccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTccg                                                          <  2:4083880/101‑2 (MQ=255)
                                      cccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTccgg                                                         >  2:2715777/1‑99 (MQ=255)
                                      cccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTccgg                                                         <  2:2714790/101‑3 (MQ=255)
                                       ccgcGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTCCGgg                                                        <  1:3097601/101‑1 (MQ=37)
                                        cgcgcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGTCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTCCGggg                                                       <  1:1061801/101‑1 (MQ=37)
                                        cgcgcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTCCGggg                                                       <  2:2942705/101‑1 (MQ=37)
                                         gcgcgGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTCCGgggg                                                      <  2:3142277/101‑1 (MQ=37)
                                         gcgcgGCATCATCGCGCTGAGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCACCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTCCGgggg                                                      >  1:4506894/1‑101 (MQ=25)
                                             ggCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGcgcg                                                 <  1:3267341/101‑1 (MQ=25)
                                               catcatCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGaa                                               >  1:4172398/1‑101 (MQ=25)
                                               catcatCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGaa                                               <  2:2567260/101‑1 (MQ=25)
                                               catcatCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGaa                                               <  1:355214/101‑1 (MQ=25)
                                               catcatCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGaa                                               <  1:1272014/101‑1 (MQ=25)
                                                atcatcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAg                                              <  1:1435733/101‑1 (MQ=25)
                                                atcatcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAg                                              <  2:2839765/101‑1 (MQ=25)
                                                atcatcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAg                                              <  2:4418778/101‑1 (MQ=25)
                                                atcatcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAg                                              <  1:1787939/101‑1 (MQ=25)
                                                  catcGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGcc                                            >  1:2437066/1‑101 (MQ=25)
                                                    tcGCGCTGCGCGGCCGCGCCGCGGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGcccc                                          <  2:2542293/101‑1 (MQ=21)
                                                    ccgcgCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGcccc                                          <  1:709695/100‑1 (MQ=21)
                                                     cgcgCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGccccc                                         <  2:4353018/101‑1 (MQ=21)
                                                     cgcgCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGccccc                                         <  2:1673744/101‑1 (MQ=21)
                                                     cgcgCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGccccc                                         <  1:591574/101‑1 (MQ=21)
                                                     cgcgCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGccccc                                         <  1:2713118/101‑1 (MQ=21)
                                                        gcTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGgc                                      >  2:2378486/1‑101 (MQ=21)
                                                         cTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGgcg                                     >  1:1745836/1‑101 (MQ=21)
                                                          tGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGccccagatcg                                    >  1:3655075/1‑95 (MQ=18)
                                                          tGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGG.CCCGGGGGCGCGAAGCCCCCGgcgc                                    >  2:4641133/1‑101 (MQ=21)
                                                              cgGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCgccgcc                                >  1:1904286/1‑101 (MQ=18)
                                                                 ccgcgccgcCGGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGa                             >  2:2285025/1‑101 (MQ=18)
                                                                      ccgccgGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCg                        >  1:1679902/1‑101 (MQ=18)
                                                                          cgGAGATTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGgaga                    >  2:4600482/1‑101 (MQ=18)
                                                                               aTTGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCg               >  1:3538474/1‑101 (MQ=18)
                                                                                ttGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGa              >  1:2689049/1‑101 (MQ=18)
                                                                                ttGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGa              >  2:3134525/1‑101 (MQ=18)
                                                                                 tGAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGAt             >  1:804257/1‑101 (MQ=18)
                                                                                  gAGCCGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGATg            >  2:2676645/1‑101 (MQ=18)
                                                                                     ccGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGATGaca         >  1:432209/1‑101 (MQ=18)
                                                                                      cGATGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGATGacac        >  2:100102/1‑101 (MQ=18)
                                                                                         tGCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGG.CCCGGGGGCGCGAAGGCCACGGCGCCGCCAGATCCCGGAGACCCCGATGAcacacc     >  2:3612573/1‑99 (MQ=21)
                                                                                          gCCCCCCGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGATGACACAGcc    >  2:138117/1‑101 (MQ=18)
                                                                                            cccccGC.CGCC.CTTTCATCTTGCCCAAAATATCCCGCGGGGGT.CCGGAGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGa  >  2:772723/1‑101 (MQ=18)
                                                                                                      |                                                                                             
TGGATGATCCTCTTCATCATCATCTTCATCCAGTTCCGCCCGCGCGGCATCATCGCGCTGCGCGGCCGCGCCGCCGGAGATTGAGCCGATGCCCCCCGG.CG.C.CTTTCATCTTGCCCAAAATATCCCGCGGGGGTCCCGGGGGCGCGAAGCCCCCGGCGCCGCCAGATCCCGGAGACCCCGATGACACAGCCGA  >  NC_014034/1300960‑1301152

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG