Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,303,931	+CA	100%	intergenic (+118/+39)	urtE → / ← RCAP_rcc01231	urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,303,931	1	.→C	100.0%	171.7	85	intergenic (+118/+39)	urtE/RCAP_rcc01231	urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (51/33):  ref base (1/0):  total (52/33)
*	NC_014034	1,303,931	2	.→A	100.0%	169.8	85	intergenic (+118/+39)	urtE/RCAP_rcc01231	urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (50/33):  ref base (1/0):  total (52/33)

TGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGG..G..G.C.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCA  >  NC_014034/1303858‑1303995                                                                                 ||                                                                   tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGgggcggg                                              <  1:2480518/101‑1 (MQ=14) tGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGgggcggg                                              >  2:169142/1‑101 (MQ=14)  ggggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGgggcgggc                                             <  2:1302747/101‑1 (MQ=14)   gggCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggcc                                            >  2:4068038/1‑101 (MQ=14)      cAAGATAACCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgca                                         <  1:2988281/101‑1 (MQ=9)       aaGATAACCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcag                                        <  2:324371/101‑1 (MQ=9)       aaGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcag                                        <  2:3513518/101‑1 (MQ=14)       aaGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcag                                        >  1:3519800/1‑101 (MQ=14)       aaGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcag                                        >  1:3361931/1‑101 (MQ=9)        aGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagg                                       >  1:4163937/1‑101 (MQ=14)        aGATAAACCCCATCCTTTCACCTTGCCGCAAATACCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagg                                       <  2:2228843/101‑1 (MQ=9)         gATAAACCCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcaggg                                      <  1:696988/101‑1 (MQ=9)         gATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCCG.CGCCCCCTTTTCGGGGCgggccgccagg                                      >  2:2901542/1‑97 (MQ=9)          aTAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAGGAGGGGG..GCAG.CGCCCCCTTTTAGGGGCgggccgcagggc                                     >  1:1387025/1‑101 (MQ=9)           tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggcc                                    >  1:3835737/1‑101 (MQ=14)           tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggcc                                    >  1:2445950/1‑101 (MQ=14)           tAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggcc                                    <  2:3334533/101‑1 (MQ=14)            aagccctgtCCTTTCGCCTTGCCGCAAAGGTTCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggccg                                   <  1:2545422/93‑1 (MQ=2)            aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggccg                                   >  2:1990618/1‑101 (MQ=14)            aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggccg                                   >  2:235268/1‑101 (MQ=14)             aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggccgc                                  >  1:951809/1‑101 (MQ=14)             aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggccgc                                  >  2:2594452/1‑101 (MQ=14)              aCCCCATCCTTTCTCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCgggccgcagggccgca                                 <  1:2532924/101‑1 (MQ=9)               ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                <  2:211985/101‑1 (MQ=14)               ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                >  1:625181/1‑101 (MQ=14)               ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGGTCA..G.CGCGCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                >  2:2830202/1‑101 (MQ=9)               ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGGACCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                >  1:3428062/1‑101 (MQ=9)                cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc                               >  2:4394458/1‑101 (MQ=14)                 ccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAccc                              >  2:468793/1‑101 (MQ=14)                  cATCCTTTCACCTTGCCGCAAATCTCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                             <  1:1934072/101‑1 (MQ=9)                  cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                             <  1:681799/101‑1 (MQ=14)                   ctCCTTTCTCGTTGCGGTATATATCCTGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGT..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCCCCCTg                            <  2:4553359/100‑1 (MQ=2)                     ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc                          >  2:2763581/1‑101 (MQ=14)                     ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc                          >  1:244201/1‑101 (MQ=14)                      cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                         >  2:3139949/1‑101 (MQ=14)                      cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                         <  2:3417652/101‑1 (MQ=14)                       tttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAg                        >  1:1922202/1‑101 (MQ=14)                       tttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAg                        <  2:3606259/101‑1 (MQ=14)                        ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                       >  1:3545773/1‑101 (MQ=14)                         tccccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                      <  1:2659521/98‑1 (MQ=9)                         tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                      <  1:672721/101‑1 (MQ=14)                         tCACCTAGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGGCGCAGGGCCGCACCCGGTCAgcg                      >  2:1984083/1‑101 (MQ=9)                          cACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcgc                     >  2:2595452/1‑101 (MQ=17)                          cACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcgc                     >  1:4546583/1‑101 (MQ=17)                           aCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGGCGCAGGGCCGGACCCTGTAAgggcc                    >  2:1834988/1‑100 (MQ=2)                            ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                   >  2:138254/1‑101 (MQ=17)                            ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                   <  1:2833865/101‑1 (MQ=17)                            ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                   <  2:2129937/101‑1 (MQ=17)                            ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                   <  1:1950939/101‑1 (MQ=17)                             cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGACAAGAGGGGG..GCAGAG.CCCCCTTTTCGGGGCGGGGCGCCGGGCCGCCCCCCGTCAGCGCATc                  >  1:1346634/1‑101 (MQ=2)                              ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCACCCTTTTCGGGGCGGGCCACAGGGCCGCACCCTGTCAGCGCATcc                 >  1:3921635/1‑101 (MQ=9)                              ttGCCGCAAATATCCCGGGGGGCGAATTGGCCGAAGGCCAAGAGGGGG..GGAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc                 >  2:3620349/1‑101 (MQ=12)                              ttGCCGAAAATAGCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc                 <  1:2308745/101‑1 (MQ=9)                                gCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcccc               >  1:4168821/1‑101 (MQ=17)                                gCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcccc               <  2:3552468/101‑1 (MQ=17)                                 ccGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCa              >  2:2137204/1‑101 (MQ=17)                                  cGCAAATATCCGGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa             <  1:240545/101‑1 (MQ=12)                                  cGCAAATATCCCGGGGGTTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa             <  2:3034055/101‑1 (MQ=12)                                  cGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa             <  1:2070820/101‑1 (MQ=17)                                  cGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa             >  2:1536373/1‑101 (MQ=17)                                  cGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa             <  2:2367764/101‑1 (MQ=17)                                  cGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGCACGCAGGGCCGCACCCCGTAAGGGCATCCCCaa             >  2:2675182/1‑101 (MQ=2)                                   gCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCCTCCCCAAg            >  2:2919270/1‑101 (MQ=12)                                   gCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAg            >  2:4312132/1‑101 (MQ=17)                                   gCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAg            <  1:3847456/101‑1 (MQ=17)                                    cAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc           >  1:2142319/1‑101 (MQ=17)                                    cAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc           >  2:3592606/1‑101 (MQ=17)                                    cAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc           >  1:611256/1‑101 (MQ=17)                                    cAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc           <  1:2563645/101‑1 (MQ=17)                                     aaaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc          >  1:2088477/1‑101 (MQ=17)                                     aaaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc          >  1:4562583/1‑101 (MQ=17)                                      aaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCACgccc         >  2:388819/1‑101 (MQ=12)                                      aaTATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgccc         >  2:1184657/1‑101 (MQ=17)                                       atatCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccg        >  1:3954970/1‑101 (MQ=17)                                       atatCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccg        <  2:1726875/101‑1 (MQ=17)                                         atCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc      >  2:2463653/1‑101 (MQ=18)                                         atCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc      >  1:2169724/1‑101 (MQ=18)                                         atCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc      >  2:1910988/1‑101 (MQ=18)                                         atCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc      >  1:55191/1‑101 (MQ=18)                                         atCCCGGGGGGTGAATTGGCCGAAGGCAAAGAGGGGG..GCAG.CTCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgcc      <  2:2520446/101‑1 (MQ=16)                                          tCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc     <  2:2789066/101‑1 (MQ=18)                                          tCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc     <  1:1331944/101‑1 (MQ=18)                                          tCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAGCCCCAAggccgccc     >  1:662158/1‑101 (MQ=12)                                          tCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGCCCGCACCCTGTCAGCGCATCCCCAAgcccgccc     >  1:3979998/1‑101 (MQ=12)                                            ccGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCccgcccgc   >  1:3638509/1‑101 (MQ=18)                                             cGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCa  >  2:2702082/1‑101 (MQ=18)                                             cGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCa  <  1:1369859/101‑1 (MQ=18)                                             cGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCa  >  1:710613/1‑101 (MQ=18)                                             cGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCa  >  2:4137729/1‑101 (MQ=18)                                             cGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAG.CGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCa  <  1:1330941/101‑1 (MQ=18)                                                                                 ||                                                                   TGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGG..G..G.C.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCA  >  NC_014034/1303858‑1303995 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG