Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,320,651 +G 100% intergenic (+109/+76) potI1 → / ← RCAP_rcc01247 polyamine ABC transporter permease PotI/membrane transport family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,320,6511.→G100.0% 493.5 154intergenic (+109/+76)potI1/RCAP_rcc01247polyamine ABC transporter permease PotI/membrane transport family protein
Reads supporting (aligned to +/- strand):  new base (88/66):  ref base (0/0):  total (88/66)

Only 100 of 174 total aligned reads displayed.

ATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.G.GCA..GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGC  >  NC_014034/1320556‑1320741
                                                                                                  |                                                                                            
aTCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCA..gggg                                                                                         <  1:1942317/101‑2 (MQ=255)
aTCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCA..gggg                                                                                         <  2:1162785/101‑2 (MQ=255)
  ccGAACCGCAGAAAGGGTGTCTGTCTGCCCACCTCTTTTGATGCGCAAAATTCGCCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCA..ggggtt                                                                                       <  2:3858700/101‑4 (MQ=37)
  ccGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCA..ggggct                                                                                       <  2:2155166/101‑4 (MQ=255)
  ccGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCA..ggggct                                                                                       <  2:3274250/101‑4 (MQ=255)
   cGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCA..ggggctg                                                                                      <  1:4135988/101‑5 (MQ=255)
    gAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCA..ggggctgc                                                                                     >  2:1486716/1‑96 (MQ=255)
    gAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCCTTTTCTGCGCAAAAATCACCCCCCCCCGATATTTTAAGAAAGGCGAAAATGAAAGG.GCA..ggggcggg                                                                                     >  2:3164389/1‑96 (MQ=255)
        cGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGgctgcgctg                                                                                  >  2:2574390/1‑101 (MQ=25)
            gAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCt                                                                              >  2:510202/1‑101 (MQ=25)
            gAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAAATCACCCCCCGAGGATATTTGAAGCAAGGGGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCt                                                                              >  1:126128/1‑101 (MQ=25)
             aaaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCtt                                                                             <  1:3554791/101‑1 (MQ=37)
              aaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTc                                                                            <  1:3256765/101‑1 (MQ=37)
              aaGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTc                                                                            <  1:819916/101‑1 (MQ=37)
                ggggggCTGTCTGCCCCCCTCTTTTGCTGCGCCAAATTCACCCCCCGAGGATATTTGAAGCAAGGGGAAACTGAAAGG.GCAG.GGGCTGCGGTGCCCTTcct                                                                          >  1:908739/1‑99 (MQ=25)
                ggggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCAt                                                                          <  1:3829157/101‑1 (MQ=37)
                ggggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCAt                                                                          >  1:646794/1‑101 (MQ=37)
                  ggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCAtat                                                                        <  1:4019632/101‑3 (MQ=37)
                  ggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCt                                                                        <  2:3574197/101‑1 (MQ=38)
                  ggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCt                                                                        <  2:3534330/101‑1 (MQ=38)
                  ggggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCt                                                                        <  2:1883337/101‑1 (MQ=38)
                   gggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCtt                                                                       <  1:2579743/101‑1 (MQ=38)
                   gggCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCtt                                                                       <  2:232718/101‑1 (MQ=38)
                       tGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCca                                                                   <  1:2249641/101‑1 (MQ=38)
                       tGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCca                                                                   <  2:1975981/101‑1 (MQ=38)
                         tCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCcaca                                                                 >  1:3865354/1‑101 (MQ=38)
                         tCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCcaca                                                                 >  2:3737672/1‑101 (MQ=38)
                         tCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGGGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCCTGCcaca                                                                 >  2:4404790/1‑101 (MQ=37)
                          cTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACaa                                                                <  1:3696878/101‑1 (MQ=38)
                           tGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACaaa                                                               >  2:1068882/1‑101 (MQ=38)
                           tGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCCAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACaaa                                                               >  2:2521716/1‑101 (MQ=38)
                            gCCCCCCTCTTTTTCTGCGCAAAATTCACCCCCCCAGGATATTTGAAGCAAGGGGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCCTCTTTCCACcaaa                                                              >  2:1065416/1‑100 (MQ=255)
                            gCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACaaaa                                                              >  2:2926170/1‑100 (MQ=38)
                              cccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GGAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAatat                                                            >  1:1896747/1‑101 (MQ=38)
                               ccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATc                                                           >  1:3946205/1‑101 (MQ=39)
                               ccccTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAACG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATc                                                           <  2:753681/101‑1 (MQ=38)
                                 ccTCTTCTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATccc                                                         <  1:748525/101‑1 (MQ=38)
                                  ctctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCg                                                        >  1:1624569/1‑101 (MQ=38)
                                   tctTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgg                                                       >  1:2363510/1‑101 (MQ=39)
                                      tttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCggggg                                                    <  2:4172532/101‑1 (MQ=38)
                                      tttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCggggg                                                    >  1:672560/1‑101 (MQ=38)
                                      tttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGAGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCggggg                                                    >  1:4539029/1‑101 (MQ=38)
                                       ttGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCgggggg                                                   <  1:2442986/101‑1 (MQ=38)
                                        tgctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGa                                                  <  1:3456382/101‑1 (MQ=38)
                                        tgctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGa                                                  <  1:1675964/101‑1 (MQ=38)
                                        tgctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGa                                                  >  1:1106069/1‑101 (MQ=38)
                                         gctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAg                                                 >  2:1862365/1‑101 (MQ=38)
                                         gctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAg                                                 >  1:405867/1‑101 (MQ=38)
                                          ctgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGAGAAACTGAAA.GCGCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGt                                                >  1:2293784/1‑101 (MQ=38)
                                           tgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTc                                               >  2:3977496/1‑101 (MQ=38)
                                           tgcGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTc                                               <  1:1486825/101‑1 (MQ=38)
                                            gcgcAAAATTCACGCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTcc                                              <  1:4112512/101‑1 (MQ=37)
                                              gcAAATTTCCCCCCCCGAGGATATTTGAACCAAGTGGAACCTGAAA.G.GGAAGGGGCTGCGCTGCCCTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCCg                                            <  2:847421/101‑1 (MQ=21)
                                                aaaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCa                                          >  2:3083631/1‑101 (MQ=37)
                                                aaaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCa                                          >  1:1255292/1‑101 (MQ=37)
                                                 aaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAg                                         >  1:2070731/1‑101 (MQ=37)
                                                 aaaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAg                                         >  1:3751007/1‑101 (MQ=37)
                                                  aaTTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAgg                                        >  1:2219987/1‑101 (MQ=37)
                                                    ttCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTACCACAAATATCCCGGCGGGAGTCCCGCAGGGa                                      <  1:4073926/101‑1 (MQ=37)
                                                     tCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAc                                     >  1:2987972/1‑101 (MQ=37)
                                                     tCACCCCCCGAGGATATTTGAAGCAAGGCGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGCGAAGTCCCGCAGGGAc                                     >  2:3682331/1‑101 (MQ=25)
                                                      cACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                   >  2:4323553/1‑101 (MQ=25)
                                                      cACCCCCCGAGGATATTTGAAGCAAGGTGAAACCGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.g                                   <  1:3978085/101‑1 (MQ=25)
                                                       aCCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gg                                  >  2:1433545/1‑101 (MQ=25)
                                                        ccccccGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggg                                 <  1:46517/101‑1 (MQ=25)
                                                         cccccGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggg                                <  1:3760853/101‑1 (MQ=25)
                                                           cccGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.gggggg                              <  2:330904/101‑2 (MQ=25)
                                                            ccGAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggc                             >  2:4439843/1‑99 (MQ=21)
                                                              gAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcag                           <  2:183136/101‑5 (MQ=21)
                                                              gAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcag                           >  1:1648712/1‑97 (MQ=21)
                                                              gAGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcag                           >  2:3124270/1‑97 (MQ=21)
                                                               aGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCCGGGAC.ggggggcagc                          >  1:1777969/1‑96 (MQ=21)
                                                               aGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcagc                          >  1:2627337/1‑96 (MQ=21)
                                                               aGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcagc                          <  1:2816451/101‑6 (MQ=21)
                                                               aGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcagc                          >  2:660001/1‑96 (MQ=21)
                                                               aGGATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.ggggggcagc                          <  1:835060/101‑6 (MQ=21)
                                                                ggATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcg                          >  1:1790722/1‑101 (MQ=18)
                                                                ggATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcg                          >  1:3194722/1‑101 (MQ=18)
                                                                 gATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcgc                         >  1:1163338/1‑101 (MQ=18)
                                                                 gATATTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAgcgc                         >  2:160974/1‑101 (MQ=18)
                                                                  atatTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                        >  1:4454440/1‑101 (MQ=18)
                                                                  atatTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGcc                        >  2:1117031/1‑101 (MQ=18)
                                                                   tatTTGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccc                       >  1:2700852/1‑101 (MQ=18)
                                                                     tttGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGccccc                     <  1:2734463/101‑1 (MQ=18)
                                                                      ttGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGAAGCGCCCCCt                    >  2:689056/1‑101 (MQ=18)
                                                                       tGAAGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCtt                   <  1:82427/101‑1 (MQ=18)
                                                                       cgatctaaGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCtt                   <  1:3766143/95‑1 (MQ=17)
                                                                         aaGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGc                 >  2:2669407/1‑101 (MQ=18)
                                                                         aaGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGc                 <  1:3056721/101‑1 (MQ=18)
                                                                          aGCAAGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCt                >  1:3893460/1‑101 (MQ=18)
                                                                             aaGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCttagatcg             >  2:3765868/1‑95 (MQ=17)
                                                                             aaGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTAc             >  2:1660191/1‑101 (MQ=18)
                                                                              aGGTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACg            <  1:4440138/101‑1 (MQ=18)
                                                                                gTGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGc          >  2:263124/1‑101 (MQ=18)
                                                                                 tGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCa         >  1:1023705/1‑101 (MQ=18)
                                                                                 tGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCa         <  2:4631849/101‑1 (MQ=18)
                                                                                 tGAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCa         <  1:2521890/101‑1 (MQ=18)
                                                                                  gAAACTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAg        >  2:2193491/1‑101 (MQ=18)
                                                                                   aaaCTGAAAGG.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAgg       >  2:3998231/1‑101 (MQ=18)
                                                                                       taaaa.G.GCAG.GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCGCCCCCTTGCTTACGGCAGGATcgc  <  1:1331046/99‑1 (MQ=18)
                                                                                                  |                                                                                            
ATCCGAACCGCAGAAAGGGGGGCTGTCTGCCCCCCTCTTTTGCTGCGCAAAATTCACCCCCCGAGGATATTTGAAGCAAGGTGAAACTGAAA.G.GCA..GGGCTGCGCTGCCCTTCATCTTGCCACAAATATCCCGGGGGGAGTCCCGCAGGGAC.GGGGGCAGCGCCCCCTTGCTTACGGCAGGATCGC  >  NC_014034/1320556‑1320741

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG