BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,946	+A	8.2%	coding (828/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,946	1	.→A	8.2%	106.6 ‑0.8 ‑0.1	343	coding (828/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (12/16): ref base (163/152): total (175/168)
Fisher's exact test strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.45e-01

Only 100 of 366 total aligned reads displayed.


CCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCG  >  NC_014034/1396846‑1397046


ccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACA.GCCT.GGGCA.Gc                                                                                                              >  2:790807/1‑101 (MQ=255)


  gcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.Cg                                                                                                           <  2:4164783/101‑1 (MQ=255)


    gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                                         <  1:2849313/101‑1 (MQ=255)


      gggGGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                                      <  1:2155617/101‑1 (MQ=255)


       ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                                     >  2:4602678/1‑101 (MQ=255)


       ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                                     >  2:2136133/1‑101 (MQ=255)


         cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                                   <  2:1144867/101‑1 (MQ=255)


         cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAA.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                                   >  2:1903913/1‑101 (MQ=255)


          gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                                  <  1:1325130/101‑1 (MQ=255)


          gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACA.GCCT.GCGCG.GG.CGCA.TCGGCt                                                                                                  >  2:1619760/1‑101 (MQ=255)


          gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACATCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                                  >  2:4036180/1‑101 (MQ=255)


             gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.Tcc                                                                                              >  1:1087713/1‑101 (MQ=255)


              aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCg                                                                                             <  2:382221/101‑1 (MQ=255)


              aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCg                                                                                             <  1:4016466/101‑1 (MQ=255)


               tCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.c                                                                                           >  1:841487/1‑101 (MQ=255)


                  aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTg                                                                                       <  1:187150/101‑1 (MQ=255)


                   aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..c                                                                                    <  2:1532053/101‑1 (MQ=255)


                     ccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..Cgg                                                                                  >  2:1347213/1‑100 (MQ=255)


                       ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTg.g                                                                               <  1:2691227/101‑1 (MQ=255)


                       ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGCg.g                                                                               >  1:3224278/1‑101 (MQ=255)


                        gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCGCGCGGGGGCGTGGATCGAAGCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CCG..CGGg.gc                                                                              >  1:3985993/1‑100 (MQ=38)


                         tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTc                                                                             <  2:463615/101‑1 (MQ=255)


                               gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTccc                                                                       >  2:4221109/1‑101 (MQ=255)


                                  aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTcccccc                                                                    <  2:1248073/101‑1 (MQ=255)


                                   gtccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCACCCcg                                                                   <  2:944517/99‑1 (MQ=255)


                                        cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                              <  2:4304358/101‑1 (MQ=255)


                                         gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCACTCCCCCCGCggggg                                                             >  1:2714616/1‑101 (MQ=255)


                                          cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                            <  1:1034042/101‑1 (MQ=255)


                                            gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                          >  2:2728398/1‑101 (MQ=255)


                                              cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                        <  2:841411/101‑1 (MQ=255)


                                               attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  2:1696901/91‑1 (MQ=0)


                                                tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  1:2244726/1‑101 (MQ=255)


                                                tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:2113141/101‑1 (MQ=255)


                                                gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  2:3143444/92‑1 (MQ=9)


                                                gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  1:477244/92‑1 (MQ=9)


                                                cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  1:3768479/9‑101 (MQ=2)


                                                cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  2:1040619/93‑1 (MQ=2)


                                                 tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                     >  2:3650505/9‑101 (MQ=2)


                                                 tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:4507913/93‑1 (MQ=9)


                                                 tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  2:258215/93‑1 (MQ=9)


                                                 gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  2:685449/94‑1 (MQ=2)


                                                 acccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  2:2478642/94‑1 (MQ=0)


                                                  cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:2026338/95‑1 (MQ=2)


                                                  cccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  2:1207063/7‑101 (MQ=2)


                                                   aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:4392637/95‑1 (MQ=9)


                                                    accctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  1:1937866/96‑1 (MQ=9)


                                                    acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  2:178334/6‑101 (MQ=9)


                                                     gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:163732/98‑1 (MQ=2)


                                                     cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  1:3730702/5‑101 (MQ=2)


                                                     aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATAGatc                                                 >  1:1879412/1‑99 (MQ=255)


                                                     aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCCCCGTGGCGTGGAGCGgaa                                                 >  2:3964688/1‑101 (MQ=37)


                                                      tgggTCGCCCCCCCGGCGGGGGCGTGGATCGAAACATGTCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  2:2528082/98‑1 (MQ=2)


                                                        cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                               <  2:2302311/101‑2 (MQ=2)


                                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                              >  2:2680248/1‑99 (MQ=9)


                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                              <  1:3073927/101‑2 (MQ=2)


                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                           <  1:3631851/101‑6 (MQ=2)


                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                            >  1:572720/1‑97 (MQ=9)


                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                           <  1:2911496/101‑6 (MQ=2)


                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                          <  2:523761/101‑7 (MQ=2)


                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCCAAAcccggc                                           >  1:869624/1‑96 (MQ=9)


                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                           >  1:974881/1‑96 (MQ=9)


                                                              tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                        >  2:2429733/1‑93 (MQ=2)


                                                              tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                        >  2:2967744/1‑93 (MQ=2)


                                                              tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActacaccgg                                        >  1:3884661/1‑93 (MQ=2)


                                                              tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                         >  2:340878/1‑94 (MQ=9)


                                                              cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                        <  1:1756856/100‑1 (MQ=255)


                                                               ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                          >  2:3748013/1‑99 (MQ=2)


                                                               ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                          <  1:247915/101‑3 (MQ=2)


                                                               ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                       <  1:1500287/101‑1 (MQ=255)


                                                               ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                        >  1:2991329/1‑93 (MQ=9)


                                                                cccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                                      >  1:2614198/1‑91 (MQ=2)


                                                                 ccccGCGGGGGCTTGGATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTC..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                     <  2:200086/101‑1 (MQ=255)


                                                                 ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                        <  2:2927158/101‑5 (MQ=2)


                                                                 ccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                                      >  1:421990/1‑91 (MQ=2)


                                                                 ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                     >  1:2293306/1‑101 (MQ=255)


                                                                 ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtc                                     >  1:722427/1‑101 (MQ=255)


                                                                 ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                      <  2:4425292/101‑11 (MQ=2)


                                                                 ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcga                                      >  1:2507218/1‑91 (MQ=2)


                                                                    cgggggggCGTGCATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                  <  2:1426817/98‑1 (MQ=255)


                                                                      cgGGGGGGTGGATCGAAACA.GCCT.GCCCG.GN.CGCA.TCGGCT.TCCT.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                <  1:1231237/101‑1 (MQ=255)


                                                                       gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                               >  2:3471174/1‑101 (MQ=255)


                                                                       gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                               >  2:2223901/1‑101 (MQ=255)


                                                                         gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                             <  2:2369390/101‑1 (MQ=255)


                                                                           gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                           >  2:2040512/1‑101 (MQ=255)


                                                                            cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                          >  1:2408764/1‑101 (MQ=255)


                                                                              tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                        <  2:3195365/101‑1 (MQ=255)


                                                                              tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                        >  2:1862839/1‑101 (MQ=255)


                                                                               ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCCGCCGGa                       >  2:1108903/1‑101 (MQ=255)


                                                                                gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                      <  1:2047772/101‑1 (MQ=255)


                                                                                     aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCTCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                 >  1:4331037/1‑101 (MQ=255)


                                                                                     aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGt                 >  1:3480855/1‑101 (MQ=255)


                                                                                      aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc                >  2:3023395/1‑101 (MQ=255)


                                                                                       aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc               <  1:3150124/101‑1 (MQ=255)


                                                                                           gCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc            >  1:3512471/1‑101 (MQ=255)


                                                                                             cT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGGCAGCCGGACGAGGTCCGTCGc          >  2:414347/1‑101 (MQ=255)


                                                                                                 cgcg.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcc       >  2:1320461/1‑101 (MQ=255)


                                                                                                  gcg.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc      >  1:1461669/1‑101 (MQ=255)


                                                                                                   cg.GC.CGCC.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCCGGCGATGTCCGTCGCTcccc     <  2:3296814/101‑1 (MQ=255)


                                                                                                    g.gC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc    >  2:3539394/1‑101 (MQ=255)


                                                                                                      gC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc   <  2:4511398/101‑1 (MQ=255)


CCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCG  >  NC_014034/1396846‑1397046

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG