BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,026	+A	14.3%	coding (908/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,026	1	.→A	14.3%	160.8 ‑2.9 ‑0.1	273	coding (908/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (17/22): ref base (143/91): total (160/113)
Fisher's exact test strand distribution p-value = 5.27e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.85e-01

Only 100 of 291 total aligned reads displayed.


GATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396926‑1397126


 aTCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                                                                                        >  2:4014005/1‑101 (MQ=255)


   cGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GAt                                                                                                      <  2:2494166/101‑1 (MQ=255)


        cAGCGTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...g                                                                                              <  2:2985757/101‑1 (MQ=255)


         aGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...Gt                                                                                             <  2:2037908/101‑1 (MQ=255)


          gCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTc                                                                                            >  1:3512471/1‑101 (MQ=255)


            cTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGc                                                                                          >  2:461799/1‑101 (MQ=255)


               cgcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcc                                                                                       >  2:1320461/1‑101 (MQ=255)


                gcgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTccc                                                                                      >  2:1256528/1‑101 (MQ=255)


                 cgGCCGCCTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCCGGC.GATGTCC...GTCGCTcccc                                                                                     <  2:3296814/101‑1 (MQ=255)


                 cgGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccc                                                                                     <  1:2018958/101‑1 (MQ=255)


                   gCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTcccccc                                                                                   <  1:2289942/101‑1 (MQ=255)


                      gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                                                <  2:756917/101‑1 (MQ=255)


                      gCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCcgcg                                                                                >  1:2411195/1‑101 (MQ=255)


                        aTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggg                                                                              >  1:2390105/1‑101 (MQ=255)


                        aTCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCggg                                                                              >  2:3046191/1‑101 (MQ=255)


                         tCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCgggg                                                                             <  1:393653/101‑1 (MQ=255)


                            gtttgcGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCg                                                                          >  1:4317809/6‑101 (MQ=255)


                            gCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCg                                                                          <  2:855754/101‑1 (MQ=255)


                            gCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCg                                                                          >  1:4543313/1‑101 (MQ=255)


                             cTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGt                                                                         <  2:2244564/101‑1 (MQ=255)


                              ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                                        >  2:931131/1‑101 (MQ=255)


                              ttCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTg                                                                        <  2:1676148/101‑1 (MQ=255)


                                ggatcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTg                                                                        >  1:4405421/11‑101 (MQ=2)


                                ccGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                                      >  1:2689549/1‑101 (MQ=255)


                                ccGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                                      >  1:462783/1‑101 (MQ=255)


                                  gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  1:1119958/101‑1 (MQ=255)


                                  gCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGCGGATc                                                                    >  1:85295/1‑101 (MQ=255)


                                  atcccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGa                                                                      >  1:1601526/9‑101 (MQ=2)


                                   ccTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  2:3512213/101‑1 (MQ=255)


                                   ccTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   >  1:3159592/1‑101 (MQ=255)


                                    cagcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    >  2:2212014/8‑101 (MQ=2)


                                    cTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                  >  1:2458862/1‑101 (MQ=255)


                                     ccgccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  2:2653565/96‑1 (MQ=2)


                                     agcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   >  1:1608934/7‑101 (MQ=2)


                                      gCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  2:375360/101‑1 (MQ=255)


                                       cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               <  1:785648/101‑1 (MQ=255)


                                       cGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               <  1:2136283/101‑1 (MQ=255)


                                        ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                >  1:3866678/4‑101 (MQ=0)


                                        ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                >  1:523809/4‑101 (MQ=0)


                                        ggcgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  2:1396239/98‑1 (MQ=0)


                                        ccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  1:1034040/99‑1 (MQ=2)


                                         tGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                             <  1:907884/101‑1 (MQ=255)


                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGTGGGCGTGGATCGAAAc                                                               <  1:4486819/100‑1 (MQ=2)


                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               >  1:1380751/2‑101 (MQ=2)


                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                               <  1:1883622/100‑1 (MQ=2)


                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcg                                                              >  1:1834991/1‑100 (MQ=2)


                                          cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                              >  2:581921/2‑100 (MQ=2)


                                           gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaa                                                             >  1:1697012/1‑99 (MQ=2)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgt                                                          >  2:2296662/1‑99 (MQ=2)


                                              gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactg                                                          >  1:1441731/1‑96 (MQ=2)


                                              ccTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTc                                                        <  1:1222609/100‑1 (MQ=255)


                                               cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgta                                                         <  1:4246383/101‑4 (MQ=2)


                                               cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgta                                                         <  2:1915654/101‑4 (MQ=2)


                                               cTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCt                                                       >  2:2844597/1‑101 (MQ=255)


                                                tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                                      <  2:4240799/101‑1 (MQ=255)


                                                tCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCtt                                                      >  2:62121/1‑101 (MQ=255)


                                                tCCCCCCGCGGGGGCGTGGATCGAAAC.CCGCCACG.TCTTGGAACAGCGTGCC.ATTGGCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaactgac                                                        >  1:622795/1‑94 (MQ=2)


                                                cccccccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtag                                                        <  1:103860/100‑5 (MQ=2)


                                                 ccccccGCAGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtaga                                                       >  2:4522955/1‑96 (MQ=2)


                                                    cccGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGcgtagatct                                                    <  1:2546810/101‑9 (MQ=2)


                                                       gcgGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGAt                                               >  1:3288056/1‑101 (MQ=255)


                                                         gggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...TTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgg                                             >  1:3702467/1‑101 (MQ=255)


                                                          ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                                            >  2:2418484/1‑101 (MQ=255)


                                                          ggggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATggg                                            >  2:706963/1‑101 (MQ=255)


                                                           gggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATgggg                                           >  1:4113737/1‑101 (MQ=255)


                                                            ggCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgc                                          <  2:1973408/101‑1 (MQ=255)


                                                              ggTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcgc                                        <  2:3602927/100‑1 (MQ=255)


                                                              cGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGgcgc                                        >  1:1484964/1‑101 (MQ=255)


                                                               gTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGcgcg                                       <  2:3222105/101‑1 (MQ=255)


                                                                  gATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGc                                    >  1:989637/1‑101 (MQ=255)


                                                                   aTCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGca                                   >  2:4348824/1‑101 (MQ=255)


                                                                     cGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGcaca                                 >  1:280764/1‑101 (MQ=255)


                                                                      gAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                >  1:2226515/1‑101 (MQ=255)


                                                                      gAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACAt                                >  2:1594751/1‑101 (MQ=255)


                                                                       aaaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCCGTCTCGCGGGACGGGGCGCCAGCACAtt                               >  2:1780033/1‑100 (MQ=37)


                                                                        aaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                              >  2:2158809/1‑101 (MQ=255)


                                                                        aaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                              >  2:1861384/1‑101 (MQ=255)


                                                                        aaCATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                              >  2:2725228/1‑101 (MQ=255)


                                                                        aaCACCTGCACG.TCGTTGGTCAGGCGGAC.GATGTGC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgt                              <  2:4536463/101‑1 (MQ=37)


                                                                           aTCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATgtcgt                           <  1:2175456/101‑1 (MQ=255)


                                                                             cAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGGGATGGGGCGCGAGCACATgtcgtgc                         >  2:314530/1‑99 (MQ=255)


                                                                               gCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCt                       <  1:4210281/101‑1 (MQ=255)


                                                                                  cg.tcgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc                    >  2:3694417/1‑101 (MQ=255)


                                                                                  cg.tcgtTGGTCAGCCGGAC.GAGGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccc                    >  1:2646522/1‑101 (MQ=255)


                                                                                   g.tcgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccc                   >  2:4145463/1‑101 (MQ=255)


                                                                                     tcgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTccccc                  >  1:4041633/1‑101 (MQ=255)


                                                                                      cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                 >  1:1977417/1‑101 (MQ=255)


                                                                                      cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                 >  2:2363721/1‑101 (MQ=255)


                                                                                      cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                 >  1:2945225/1‑101 (MQ=255)


                                                                                      cgtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTcccccc                 <  2:4448888/101‑1 (MQ=255)


                                                                                       gtTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcg                >  2:4468523/1‑101 (MQ=255)


                                                                                         tGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGTATCGAAACTGCCGTCTTGACTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgcg              >  1:3983871/1‑101 (MQ=255)


                                                                                         tGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgcg              >  2:4094792/1‑101 (MQ=255)


                                                                                         tGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgcg              >  1:3085845/1‑101 (MQ=255)


                                                                                         tGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgcg              >  1:3586265/1‑101 (MQ=255)


                                                                                         tGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCcgcg              >  1:3385921/1‑101 (MQ=255)


                                                                                                ccGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGt       <  1:1320554/101‑1 (MQ=255)


                                                                                                 cGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTg      <  1:4186662/101‑1 (MQ=255)


                                                                                                  ggAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTgg     >  2:1183170/1‑101 (MQ=255)


                                                                                                    aC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGAt   <  1:2076375/101‑1 (MQ=255)


GATCGAAACAGCCTGCGCGGCCGCATCGGCTTCCGCCTGCGTGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCC...GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396926‑1397126

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG