Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,208 +C 2.9% coding (1090/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2081.→C 2.9% 10.5 ‑0.9 ‑0.5209coding (1090/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (109/94):  total (111/98)
Fisher's exact test strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.47e-01

Only 100 of 238 total aligned reads displayed.

CCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCC  >  NC_014034/1397108‑1397308
                                                                                                     |                                                                                                    
 cccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.c                                                                                                     <  1:940058/101‑1 (MQ=255)
  ccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cg                                                                                                    <  2:482053/101‑1 (MQ=255)
    gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                                  <  1:144558/101‑1 (MQ=255)
    gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                                  <  1:3269160/101‑1 (MQ=255)
    gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                                                                  <  2:3079885/101‑1 (MQ=255)
     cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgacg                                                                                                 >  1:3692533/1‑101 (MQ=255)
     cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCCTGCGCCGTCCCTGCCCCCGCGGGGGCGTGGATCGAAACGTGTCCGCCa.ctccc                                                                                                 >  2:1113762/1‑97 (MQ=38)
      gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGc                                                                                                >  1:636298/1‑101 (MQ=255)
      gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGc                                                                                                <  2:4204438/101‑1 (MQ=255)
         ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                                                                             <  1:62127/101‑1 (MQ=255)
         ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                                                                             >  1:4333536/1‑101 (MQ=255)
         ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                                                                             <  1:3538228/101‑1 (MQ=255)
         ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                                                                             <  2:671398/101‑1 (MQ=255)
          gCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTg                                                                                            <  2:3686347/101‑1 (MQ=255)
             ctgATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAc                                                                                         <  1:3062956/99‑1 (MQ=255)
              ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                                                                        <  2:3853943/101‑1 (MQ=255)
               gATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCa                                                                                       >  2:414064/1‑101 (MQ=255)
                  cGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGAt                                                                                    <  2:1531576/101‑1 (MQ=255)
                   gAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATc                                                                                   >  2:2721376/1‑101 (MQ=255)
                    aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                                                                  >  1:3242167/1‑101 (MQ=255)
                    aaaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                                                                  <  1:1737905/101‑1 (MQ=255)
                     aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                                                                 <  1:2424498/101‑1 (MQ=255)
                     aaCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                                                                 <  1:2040652/101‑1 (MQ=255)
                      aCGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACGCGTCTGCCA.CGACGCCCTGCACCAGATCcgc                                                                                <  1:4145751/101‑1 (MQ=255)
                       cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                               >  1:2982545/1‑101 (MQ=255)
                       cGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgcg                                                                               <  2:3287811/101‑1 (MQ=255)
                        gTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCgcgc                                                                              <  1:876888/101‑1 (MQ=255)
                         tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                                                             >  1:271669/1‑101 (MQ=255)
                           cAGGTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGt                                                                           >  2:3953226/1‑101 (MQ=255)
                           cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGt                                                                           <  2:2785504/101‑1 (MQ=255)
                           cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGt                                                                           <  1:2250011/101‑1 (MQ=255)
                            cgCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                                                                          <  1:2620566/100‑1 (MQ=255)
                            aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                                                                          <  1:1027333/101‑1 (MQ=255)
                            aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                                                                          >  1:1200884/1‑101 (MQ=255)
                            aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                                                                          >  2:2474732/1‑101 (MQ=255)
                               tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CAACGCCCTGCACCAGATCCGCGCCGTCGCt                                                                       >  2:755258/1‑101 (MQ=255)
                               ccgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACGTGTCCGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCt                                                                       <  1:1610737/100‑1 (MQ=255)
                                cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGGTCCAAACGTGTCTGCCA.CGACGCCCTGCCCAAGATCCGCGCCGTCGCTc                                                                      >  2:352181/1‑101 (MQ=38)
                                  cgcCGCTTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                                                                    <  2:161333/101‑1 (MQ=255)
                                  cgcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                                                                    >  1:4072016/1‑101 (MQ=255)
                                   gcCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTTTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccc                                                                   >  1:3654384/1‑101 (MQ=255)
                                    ccACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccccc                                                                  <  2:943982/101‑1 (MQ=255)
                                     cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                                                                 <  2:2983696/101‑1 (MQ=255)
                                     cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                                                                 >  1:724954/1‑101 (MQ=255)
                                     cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                                                                 <  2:378742/101‑1 (MQ=255)
                                       caTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgc                                                               >  2:4627473/1‑101 (MQ=255)
                                         tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgg                                                             >  1:2008477/1‑101 (MQ=255)
                                           tCTTCGCCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCgggg                                                           <  2:2112615/101‑1 (MQ=255)
                                                 aCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                     <  2:1767485/101‑1 (MQ=255)
                                                    gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  >  2:3201776/1‑101 (MQ=255)
                                                      gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATTTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  2:1981517/101‑1 (MQ=2)
                                                      gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  2:1403129/101‑1 (MQ=2)
                                                       cgcCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                               <  2:2157543/101‑1 (MQ=255)
                                                        gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              >  2:2855815/1‑101 (MQ=255)
                                                        gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              >  2:4000184/1‑101 (MQ=255)
                                                         ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                             <  1:2739976/101‑1 (MQ=255)
                                                         ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                             <  2:151250/101‑1 (MQ=255)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                          <  1:4468818/101‑1 (MQ=255)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcg                                         >  2:4298586/1‑101 (MQ=255)
                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                        <  1:2363883/101‑1 (MQ=255)
                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                        <  2:3114508/101‑1 (MQ=255)
                                                               cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                       >  1:1300903/1‑101 (MQ=255)
                                                               cTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgcg                                       <  1:2315981/101‑1 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACACCgcgc                                      >  1:4403976/1‑101 (MQ=255)
                                                                cccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCgcgc                                      <  1:1113851/100‑1 (MQ=255)
                                                                  cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAc                                    >  1:121534/1‑101 (MQ=255)
                                                                   ccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcg                                   >  2:3327137/1‑101 (MQ=255)
                                                                    cccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCAcgc                                  <  2:2408602/101‑1 (MQ=255)
                                                                           gggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                           <  2:1477341/101‑1 (MQ=255)
                                                                           gggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACg                           <  1:1783790/101‑1 (MQ=255)
                                                                            ggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACgg                          <  2:4070452/101‑1 (MQ=255)
                                                                               gTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCCGCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                       <  1:4001838/101‑1 (MQ=255)
                                                                               gTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                       >  1:3376478/1‑101 (MQ=255)
                                                                               gTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGt                       >  1:1496174/1‑101 (MQ=255)
                                                                                tGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                      >  1:2217304/1‑101 (MQ=255)
                                                                                tGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTc                      <  2:887626/101‑1 (MQ=255)
                                                                                tGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACACCGCGCACGCGCGAACGGCGTc                      >  1:4044104/1‑101 (MQ=255)
                                                                                 ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCggcg                     >  1:1933650/1‑98 (MQ=255)
                                                                                 ggATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCa                     <  2:1065409/101‑1 (MQ=255)
                                                                                  gATCGAAACGTGTCTGCCC.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAg                    <  2:627354/101‑1 (MQ=255)
                                                                                  gATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAg                    >  1:4114875/1‑101 (MQ=255)
                                                                                   aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                   >  1:3455723/1‑101 (MQ=255)
                                                                                   aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                   >  2:25788/1‑101 (MQ=255)
                                                                                   aTCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGc                   >  2:137047/1‑101 (MQ=255)
                                                                                     cGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGccc                 >  1:75549/1‑101 (MQ=255)
                                                                                      gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                <  1:1088438/101‑1 (MQ=255)
                                                                                      gAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGcccc                >  1:4504586/1‑101 (MQ=255)
                                                                                       aaaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcg               >  2:1593810/1‑101 (MQ=255)
                                                                                        aaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCcgc              >  2:4528294/1‑101 (MQ=255)
                                                                                        aaCGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACAGCGCACGCGCGAACGGCGTCAGCCCcgc              >  2:4558397/1‑101 (MQ=255)
                                                                                           gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa           <  2:3165458/101‑1 (MQ=255)
                                                                                           gtgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGaa           >  2:4268317/1‑101 (MQ=255)
                                                                                            tgtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAAc          >  1:4256501/1‑101 (MQ=255)
                                                                                             gtCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACg         <  2:2518638/101‑1 (MQ=255)
                                                                                               cTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGACCGTc       >  2:653502/1‑101 (MQ=255)
                                                                                                  ccA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGcc    >  1:834746/1‑100 (MQ=255)
                                                                                                  ccA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCt    >  1:4638254/1‑101 (MQ=255)
                                                                                                   cA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc   <  2:2658549/101‑1 (MQ=255)
                                                                                                   cA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTc   <  2:1142057/101‑1 (MQ=255)
                                                                                                    a.cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTcc  >  1:3866418/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
CCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGCGCACGCGCGAACGGCGTCAGCCCCGCGAACGTCGCTCC  >  NC_014034/1397108‑1397308

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG