Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,606,281 +T 2.2% coding (13/1083 nt) mraY → phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,606,2811.→T 2.2% 8.9 ‑3.9 ‑0.1179coding (13/1083 nt)mraYphospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (64/111):  total (68/111)
Fisher's exact test strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.89e-01

Only 100 of 219 total aligned reads displayed.

CGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTCCT  >  NC_014034/1606181‑1606381
                                                                                                     |                                                                                                       
cGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGc                                                                                                          <  1:703500/101‑1 (MQ=255)
cGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGc                                                                                                          <  2:1084119/101‑1 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.t                                                                                                        >  1:3716868/1‑101 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.t                                                                                                        >  1:2290684/1‑101 (MQ=255)
 gAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.t                                                                                                        <  2:2557801/101‑1 (MQ=255)
   aaGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTg                                                                                                      <  1:2052198/101‑1 (MQ=255)
    aGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGc                                                                                                     <  1:395930/101‑1 (MQ=255)
    aGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGc                                                                                                     <  1:738503/101‑1 (MQ=255)
     ggCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttt                                                                                                    >  1:3274471/1‑98 (MQ=255)
     ggCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.g                                                                                                   <  2:1727438/101‑1 (MQ=255)
       cTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.tttttta                                                                                                  >  2:4522164/1‑96 (MQ=255)
        tCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.tttttgac                                                                                                 >  2:3560297/1‑95 (MQ=255)
        tCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttcgac                                                                                                 >  2:2460354/1‑95 (MQ=255)
        tCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAAc                                                                                                <  1:1013872/101‑1 (MQ=255)
         cGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttttaac                                                                                                >  1:3768030/1‑94 (MQ=255)
         cGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttgctac                                                                                                >  2:1572469/1‑94 (MQ=255)
          gAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttgtgcct                                                                                               >  1:3938481/1‑93 (MQ=255)
          gAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttgtagct                                                                                               >  1:2151941/1‑93 (MQ=255)
          gAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttgatcac                                                                                               >  2:4167432/1‑93 (MQ=255)
           aaGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttttatctt                                                                                              >  2:2785707/1‑92 (MQ=255)
           aaGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.ttttttaccct                                                                                              >  1:3442187/1‑92 (MQ=255)
           aaGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.tttttgtccct                                                                                              >  2:3438405/1‑92 (MQ=255)
           aaGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GTACC.tt                                                                                            >  1:977018/1‑101 (MQ=255)
           aaGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.tt                                                                                            <  2:823205/101‑1 (MQ=255)
            aGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GAACC.tct                                                                                           >  2:2387690/1‑99 (MQ=255)
             ggTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTc                                                                                          <  1:4191339/101‑1 (MQ=255)
             ggTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTc                                                                                          <  1:1631174/101‑1 (MQ=255)
              gTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.g                                                                                        <  1:2785905/101‑1 (MQ=255)
              gTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.g                                                                                        <  2:2861474/101‑1 (MQ=255)
              gTTTCGCTCGGGGTTGACGCGCTCAGAAATCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.g                                                                                        <  2:3042881/101‑1 (MQ=255)
               tttCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.gg                                                                                       <  1:144990/101‑1 (MQ=255)
                 tCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGAc                                                                                     <  1:4577484/101‑1 (MQ=255)
                 tCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGAc                                                                                     <  2:4431446/101‑1 (MQ=255)
                  cGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GGACC.ttttcgtcg                                                                                     >  1:1145141/1‑95 (MQ=255)
                     tCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACgggg                                                                                 <  2:4243349/101‑1 (MQ=255)
                      cGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTC.GTACC.TTTC.TGGCggggg                                                                                >  1:2350238/1‑101 (MQ=255)
                       gTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGc                                                                               <  1:3283181/101‑1 (MQ=255)
                       gTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGc                                                                               <  1:2477583/101‑1 (MQ=255)
                        tGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GTACC.TTTT.TGACggggggg                                                                              >  1:1990664/1‑99 (MQ=255)
                         ggTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGCTTTTG.GAACC.TTT..GGAGgggggggg                                                                             >  1:1536871/1‑98 (MQ=255)
                          gTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GAAAC.TTTT.CGACggggggggg                                                                            >  2:3748538/1‑97 (MQ=255)
                          gTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGAt                                                                            <  2:3239308/101‑1 (MQ=255)
                            tGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGAttt                                                                          <  1:1928542/101‑1 (MQ=255)
                              aCGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.TAACC.CTTT.GGCCgggggggggtgtt                                                                        >  1:4622918/1‑93 (MQ=255)
                                gcgcTGAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATttcttc                                                                      <  2:4143844/101‑1 (MQ=255)
                                gcgcTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATttcttc                                                                      <  2:2963520/101‑1 (MQ=255)
                                 cgcTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCa                                                                     <  1:539676/101‑1 (MQ=255)
                                 cgcTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATCCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCa                                                                     <  1:2678512/101‑1 (MQ=255)
                                     cAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACct                                                                 <  2:1475781/101‑1 (MQ=255)
                                        aaaaCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCtt                                                              <  2:3767755/101‑1 (MQ=255)
                                         aaaCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTc                                                             <  1:4344616/101‑1 (MQ=255)
                                         aaaCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTc                                                             <  1:236995/101‑1 (MQ=255)
                                         aaaCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAAAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTc                                                             <  2:1834195/101‑1 (MQ=255)
                                          aaCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTcc                                                            <  1:2242187/101‑1 (MQ=255)
                                          aaCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTcc                                                            <  1:2870471/101‑1 (MQ=255)
                                           aCTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCg                                                           <  2:1618435/101‑1 (MQ=255)
                                             tCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCt                                                         <  2:3530224/101‑1 (MQ=255)
                                             tCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCt                                                         <  2:2039196/101‑1 (MQ=255)
                                                gTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GGACC.TTTT.GGACGGGGGGGGGTTCTTTCACCCCTTCCCCtccc                                                      >  1:150089/1‑98 (MQ=255)
                                                 tCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAt                                                     <  1:3034551/101‑1 (MQ=255)
                                                 tCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAt                                                     <  1:3346409/101‑1 (MQ=255)
                                                 tCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACAt                                                     <  1:3560555/101‑1 (MQ=255)
                                                   aGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GGGAC.TTTC.TGGCGGGGGGGGGTTCTTCCACACCTTCCCCTGCACCa                                                   >  1:2635629/1‑101 (MQ=255)
                                                      ccGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCt                                                <  2:509645/101‑1 (MQ=255)
                                                      ccGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCt                                                <  1:4522459/101‑1 (MQ=255)
                                                      ccGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCt                                                <  1:4280956/101‑1 (MQ=255)
                                                       cGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAAGC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCtt                                               <  2:4033782/101‑1 (MQ=255)
                                                        ggCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTc                                              <  2:1800909/101‑1 (MQ=255)
                                                         gcgcGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTcc                                             <  2:407928/101‑1 (MQ=255)
                                                         gcgcGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTACGCTACATCACCTTcc                                             <  1:1771726/101‑1 (MQ=255)
                                                          cgcgGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCcg                                            <  2:231250/101‑1 (MQ=255)
                                                             ggCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GAACC.TTTT.GGGCGGGGGGGGTTTTTTTTACCTCTTCCCCTACATCACCTTCCccgc                                         >  1:3525220/1‑101 (MQ=255)
                                                                agagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GGACC.TTTT.GGACGGGGGGGGTTTTTTTTAACTCTTTCCCTACATACCCTTCCCCGCCgc                                      >  1:4456716/1‑100 (MQ=255)
                                                                agagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GAACC.CTTCTGGCCGGGGGGGGTTTTTTAAACCCCTTCC.CCGCAGCACCTTCCGCGcccc                                      >  2:3832531/1‑99 (MQ=255)
                                                                agagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTC.GGACC.TTTT.GGACGGGGGGGGTTTTTTCAACCTCTTCCCCTCCATTCCCCTCCCCGcccg                                      >  2:1607272/1‑99 (MQ=255)
                                                                agagGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.CAACC.TTTC.CGGCGGGGGCGGTGTCTTTAAACTCCTCGGCTACACCACCCTCCTCGCCgc                                      >  2:281816/1‑100 (MQ=255)
                                                                    gCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTC.GAACC.TTTT.CGGCGGGGGGGGTTTTTTTACCCCTTTCCCCTACATTACCTTCCCCGCCCGTggg                                  >  1:644966/1‑101 (MQ=255)
                                                                     ccTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTT.GAACC.TTTT.GGGCGGGGGGGGTTTTTTTAACCTCTTTCCCTACATCACCTTCCCCCCCCGGgggg                                 >  1:2432990/1‑101 (MQ=255)
                                                                      cTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTTC.GAACC.TTTT.GGACGGGGGGGGTTTTTTTAACCTCTTCCCCTACATCACCTTCCCCGCCGGGggggg                                >  1:2586680/1‑100 (MQ=255)
                                                                       ttCAAGGGAAAAGAATAATGCTTTACTGGC.TTTC.GAACC.CTTT.GGACGGGGGGGGTTTTTTTAACCTCTTTCCCTACATCACCTTCCCCCCCCGGgggggc                               >  1:1114947/1‑99 (MQ=255)
                                                                        tCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCct                              <  2:331135/101‑1 (MQ=255)
                                                                         cAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCctt                             <  2:2011367/101‑1 (MQ=255)
                                                                          aaGGGAAAAGAATAATGCTTTACTGGCTTTTG.GACCC.TTTC.GG.CGGGGGGGGGTTCTTCAACCTCCTCCCCTACATCCCCCTCCCCGCCCGCGGGGGcttt                            >  2:1868482/1‑100 (MQ=255)
                                                                          aaGGGAAAAGAATAATGCTTTACTGGC.TTTC.GAACC.CTTT.GGGCGGGGGGGGTTTTTTTTCACTCTTCCCCTACATCACCTTCCCCCCCCGGGGGGcccct                            >  1:3161627/1‑98 (MQ=255)
                                                                            gggAAAAGAATAATGCTTTACTGGC.TTTG.GAACC.CTTT.AGACGGGGGCGATTTTTTTAACCTCTTCCCCTTCATCACCTTCCGCCCCGGTGGGGCgtttct                          >  1:1231159/1‑98 (MQ=255)
                                                                              gAAAAGAATAATGCTTTACTGGC.TTTCGGACCC.TTTC.GG.CGGGGGGGGTTTTTTTAACCCCTTCCCCTACCTCACCTTCCCCGCCCGTGGGGGCctcttcc                        >  1:135427/1‑100 (MQ=255)
                                                                               aaaaGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGGGGTTTTTTCAACCTCTTCCGCTACATCACCTTCCGCGCCCGTGGGGCCTTCTTCAc                       >  2:3941448/1‑101 (MQ=255)
                                                                                aaaGAATAATGCTTTACTGGCTTTGG.AAACC.TTTG.GGCGGGGGGGGTTTTTTTAAACCCCTT.CGCCTCATAACCCTCCCCGCCGGGGGGGGgttcttgtcc                      >  2:2297104/1‑97 (MQ=255)
                                                                                aaaGAATAATGCTTTACTGGC.TTTC.GAACCTTTTC.GGCCGGGGG.GGTTTCTTCAACCTCTTTCCCCACAACACCTTTCCCGCCCGTGGGGGCTTCTTCTcc                      >  2:723394/1‑101 (MQ=255)
                                                                                   gAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACcgcg                   <  1:922801/101‑1 (MQ=255)
                                                                                      taatGCTTTACTGGC.TTGC.GAACC.TTTC.GGGCGGGGGGGGTTTTTTCAAACTCTTCCGCTACATCACCTTCCGCGCCCGGGGGGCCTTCTTTACCCCGCTg                >  2:1691942/1‑101 (MQ=255)
                                                                                       aatGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGGGGTTTTTTCAACCTCTTCCGCTACATCAACTTCCCCGCCCGTGGGGGCTTCTTTACCGCGCTGa               >  2:26956/1‑101 (MQ=255)
                                                                                         tGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATc             <  2:2200822/101‑1 (MQ=255)
                                                                                           cTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCCCCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCtt           <  1:3516176/101‑1 (MQ=255)
                                                                                           cTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCtt           <  2:115478/101‑1 (MQ=255)
                                                                                            tttACTGGC.TTGC.GAAGC.TTTG.GGACGGGGGCGATTTCTACGACCTCTTCCGCTACATCACCGTCCGCGCCGGTGCGGCCTTCTTCAACGCGCTGATCTTc          <  1:3103432/101‑1 (MQ=255)
                                                                                            tttACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTc          <  1:1242358/101‑1 (MQ=255)
                                                                                              tACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCgg        <  2:4324312/101‑1 (MQ=255)
                                                                                                  ggC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACCCGCCTGACCTTCGCCTTc    >  1:231128/1‑101 (MQ=255)
                                                                                                    c.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTCCt  >  1:3815499/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
CGAAAGGCTCGAAGGTTTCGCTCGTGGTTGACGCGCTCAGAAAACTCGGTCAGCCCGGCGCGGCAGAGGCCTTCAAGGGAAAAGAATAATGCTTTACTGGC.TTGC.GAACC.TTTC.GGACGGGGGCGATTTCTTCAACCTCTTCCGCTACATCACCTTCCGCGCCGGTGGGGCCTTCTTCACCGCGCTGATCTTCGGCTTCCT  >  NC_014034/1606181‑1606381

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG