Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,891 +A 4.2% intergenic (‑144/+49) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8911.→A 4.2% 25.0 ‑0.3 ‑0.2190intergenic (‑144/+49)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (93/89):  total (98/92)
Fisher's exact test strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.36e-01

Only 100 of 209 total aligned reads displayed.

AGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCG  >  NC_014034/1734792‑1734990
                                                                                                       |                                                                                                    
 gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCATGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.g                                                                                                     <  1:1763434/101‑1 (MQ=255)
 gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.g                                                                                                     <  1:1580250/101‑1 (MQ=255)
  ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.gg                                                                                                    >  1:66097/1‑101 (MQ=255)
    aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGcc                                                                                                  >  1:4395081/1‑101 (MQ=255)
    aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGcc                                                                                                  >  2:197414/1‑101 (MQ=255)
    aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGcc                                                                                                  >  2:2059384/1‑101 (MQ=255)
       ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCCGGGCAATTATGGCAAGAATAAAATGGC.GGCCtc                                                                                                >  1:5823/1‑100 (MQ=17)
        gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCa                                                                                              <  2:910693/101‑1 (MQ=255)
         cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCAt                                                                                             <  2:2530820/101‑1 (MQ=255)
         cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCAGGATATGTATGGCAAG.ATGGAACTGC.GGCCTTCAt                                                                                             >  2:1880036/1‑101 (MQ=18)
          tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCCTCAtt                                                                                            >  1:40369/1‑100 (MQ=255)
           gccccTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAT.ATGAAACTGC.GGCCTTCATCt                                                                                           <  2:1116824/97‑1 (MQ=18)
           gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCt                                                                                           <  1:732751/101‑1 (MQ=255)
            aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTCC.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCtt                                                                                          <  1:3938117/101‑1 (MQ=255)
             cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTg                                                                                         <  1:4629208/101‑1 (MQ=255)
              gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGc                                                                                        <  2:1366481/101‑1 (MQ=255)
              gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGc                                                                                        >  2:4465189/1‑101 (MQ=255)
              gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGc                                                                                        <  1:3065265/101‑1 (MQ=255)
                  cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                    >  1:2294734/1‑101 (MQ=255)
                  cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                    <  2:3397376/101‑1 (MQ=255)
                  cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                    >  1:474469/1‑101 (MQ=255)
                    tCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                  >  1:3719863/1‑101 (MQ=255)
                     cAGCCGTCGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                 <  2:3681062/101‑1 (MQ=255)
                     cAGCCGACGGGCCGGGCAGGGGGGCGGG.GCCCCCCGTCCGGCGGACTCCCCCCCGTTTATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                 >  2:2328859/1‑101 (MQ=17)
                     cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                 >  2:6950/1‑101 (MQ=255)
                     cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                 >  1:1167391/1‑101 (MQ=255)
                      aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                >  2:2922739/1‑101 (MQ=255)
                      aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                >  2:1428254/1‑101 (MQ=255)
                         cGACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                             <  2:372355/101‑1 (MQ=255)
                          gACGGGCCGGGCAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTCCATCTTGCCATAAATATccc                                                                            >  1:295962/1‑101 (MQ=255)
                             gggccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATTCC.ggg                                                                        >  2:35097/1‑101 (MQ=255)
                               tgacggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                     >  1:2761741/9‑101 (MQ=2)
                               gccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                                      <  2:3253867/101‑1 (MQ=255)
                               gccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                                      <  2:416160/101‑1 (MQ=255)
                                ccgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                     <  2:3784874/101‑1 (MQ=255)
                                 cgggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                    >  1:663736/1‑101 (MQ=255)
                                 acggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                   <  2:4332654/95‑3 (MQ=2)
                                 acggacggggggCTCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                   >  2:4309734/7‑99 (MQ=2)
                                  gggcAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                   <  1:3864913/101‑1 (MQ=255)
                                    gacggggggCGCT.G.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaattg                                                                <  1:3012753/98‑6 (MQ=9)
                                     cAGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                                >  1:4273313/1‑101 (MQ=255)
                                      aGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                               >  2:4078406/1‑101 (MQ=255)
                                      aGGGGGGCGCT.GCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                               <  2:1640858/101‑1 (MQ=255)
                                               gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                       <  2:3867039/96‑1 (MQ=255)
                                                 tgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                                                     >  1:4439373/4‑101 (MQ=255)
                                                  gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                    <  1:1021295/99‑1 (MQ=255)
                                                   ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                   <  2:10326/100‑1 (MQ=255)
                                                    cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                  <  1:1724979/101‑1 (MQ=255)
                                                     ccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                 <  1:1097123/101‑1 (MQ=255)
                                                     ccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                 >  2:4560387/1‑101 (MQ=255)
                                                     ccccGGCCTGCGGGCTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                 <  1:2431716/101‑1 (MQ=255)
                                                      cccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAc                                                >  2:159375/1‑101 (MQ=255)
                                                       ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                               >  2:122683/1‑101 (MQ=255)
                                                       ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAAcc                                               >  2:1020723/1‑101 (MQ=255)
                                                            cTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGa                                          <  1:1192006/101‑1 (MQ=255)
                                                             tGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                         <  2:2105691/101‑1 (MQ=255)
                                                                  gcTCCCCCCGGGATATTTAGGGCAAG.ATGAACCTGC.GGCCTTCATCTTGCGATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                    <  1:2380043/100‑1 (MQ=255)
                                                                  aCTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                    >  2:3647358/1‑101 (MQ=255)
                                                                  aCTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                    <  1:2077801/101‑1 (MQ=255)
                                                                     ccccccGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                 <  1:2826345/101‑1 (MQ=255)
                                                                        cccGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                              >  1:1485187/1‑101 (MQ=255)
                                                                        cccGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg                              <  1:2509553/101‑1 (MQ=255)
                                                                         ccGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                             <  1:3378216/101‑1 (MQ=255)
                                                                         ccGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                             <  2:4373336/101‑1 (MQ=255)
                                                                         ccGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                             <  1:3897627/101‑1 (MQ=255)
                                                                          cGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                            >  2:3140917/1‑101 (MQ=255)
                                                                          cGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                            >  2:594725/1‑101 (MQ=255)
                                                                           gggATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                           >  2:908707/1‑101 (MQ=255)
                                                                           gggATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                           >  2:1611064/1‑101 (MQ=255)
                                                                           gggATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                           >  1:3845935/1‑101 (MQ=255)
                                                                           gggATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                           >  2:4568177/1‑101 (MQ=255)
                                                                           gggATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATACGCgg                           >  2:1275148/1‑101 (MQ=255)
                                                                           gggATATTTATGGCAAG.ATGAAACTGC.GGCCATCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCAAAGATGACAAGATCCGCgg                           >  1:2629682/1‑101 (MQ=255)
                                                                            ggATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGc                          >  1:1867227/1‑101 (MQ=255)
                                                                              atatTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                        <  1:2169977/101‑1 (MQ=255)
                                                                                atTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGGCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                      >  1:2107108/1‑101 (MQ=255)
                                                                                atTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                      >  1:3142166/1‑101 (MQ=255)
                                                                                 tttATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                     >  2:3824198/1‑101 (MQ=255)
                                                                                 tttATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                     >  1:912657/1‑101 (MQ=255)
                                                                                   tATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                   >  1:1369339/1‑101 (MQ=255)
                                                                                    aTGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                  >  2:517194/1‑101 (MQ=255)
                                                                                     tGGCAAT.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                 <  2:410099/101‑1 (MQ=255)
                                                                                     tGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTc                 <  2:2373514/101‑1 (MQ=255)
                                                                                        cAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt              >  2:4586482/1‑101 (MQ=255)
                                                                                         aaG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg             >  2:271040/1‑101 (MQ=255)
                                                                                          aG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga            >  1:907959/1‑101 (MQ=255)
                                                                                           g.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat           >  2:2120534/1‑101 (MQ=255)
                                                                                             aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg          <  2:1753445/101‑1 (MQ=255)
                                                                                              tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga         >  2:1486800/1‑101 (MQ=255)
                                                                                              tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga         >  1:4166847/1‑101 (MQ=255)
                                                                                               gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg        <  2:40363/101‑1 (MQ=255)
                                                                                               gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg        >  2:4514987/1‑101 (MQ=255)
                                                                                               gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg        <  2:2387437/101‑1 (MQ=255)
                                                                                                 aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa      <  1:1114439/101‑1 (MQ=255)
                                                                                                 aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa      <  2:663674/101‑1 (MQ=255)
                                                                                                  aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa     >  1:1409056/1‑101 (MQ=255)
                                                                                                   cTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg    <  2:2934946/101‑1 (MQ=255)
                                                                                                    tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc   >  2:284747/1‑101 (MQ=255)
                                                                                                    tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGGTTTCGCTGATGAGCAAgc   >  2:1079233/1‑101 (MQ=255)
                                                                                                     gC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcg  <  2:337959/101‑1 (MQ=255)
                                                                                                       |                                                                                                    
AGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCG  >  NC_014034/1734792‑1734990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG