BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,740,769	+G	96.9%	intergenic (+6/+129)	cxp → / ← RCAP_rcc01610	thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,740,769	1	.→G	96.9%	8.0 ‑1.7 ‑0.1	160	intergenic (+6/+129)	cxp/RCAP_rcc01610	thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand): new base (85/70): ref base (1/4): total (86/74)
Fisher's exact test strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.93e-01

Only 100 of 193 total aligned reads displayed.


CGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.GGG.GCGCTGCCCCCCGCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGG  >  NC_014034/1740670‑1740864


cgAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.g                                                                                                 >  1:1411377/1‑101 (MQ=255)


    aCGATGGTGCCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.ggg.gg                                                                                            <  2:4069575/101‑2 (MQ=255)


     cGATCGGGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.ggg.ggc                                                                                           <  1:878922/101‑3 (MQ=255)


      gATGGTGACCCCCGGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.ggg.ggcg                                                                                          <  1:1825994/101‑4 (MQ=255)


       aTCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.ggg.ggcgc                                                                                         >  1:2059918/1‑97 (MQ=255)


        tCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.ggg.ggcgct                                                                                        <  1:2312439/101‑6 (MQ=255)


         cGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTg                                                                                        >  1:3951452/1‑101 (MQ=255)


          gTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGc                                                                                       >  2:636635/1‑101 (MQ=255)


           tGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGcc                                                                                      <  1:453437/101‑1 (MQ=255)


            gACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGccc                                                                                     >  1:442881/1‑101 (MQ=255)


            gACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGccc                                                                                     >  2:594461/1‑101 (MQ=255)


             aCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGcccc                                                                                    >  2:4247619/1‑101 (MQ=255)


             aCCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGcccc                                                                                    <  2:3413818/101‑1 (MQ=255)


              ccGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGccccc                                                                                   >  1:1234701/1‑101 (MQ=255)


               cGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGcccccg                                                                                  <  2:2700632/101‑2 (MQ=255)


                 ccTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGcccccgcc                                                                                >  2:127737/1‑98 (MQ=255)


                  cTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGcccccgccg                                                                               >  2:3221426/1‑97 (MQ=255)


                  cTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGcccccgccg                                                                               >  2:1402948/1‑97 (MQ=255)


                   tGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGcccccgccga                                                                              >  2:2223142/1‑96 (MQ=255)


                    gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAg                                                                             >  2:203390/1‑101 (MQ=255)


                          tCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATg                                                                       >  1:4495791/1‑101 (MQ=255)


                          tCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATg                                                                       <  2:3316817/101‑1 (MQ=255)


                           cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGc                                                                      <  2:2371618/101‑1 (MQ=255)


                           cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGc                                                                      >  2:3350233/1‑101 (MQ=255)


                             aGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCt                                                                    >  2:4543973/1‑101 (MQ=255)


                              gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTc                                                                   >  2:489613/1‑101 (MQ=255)


                              gCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTc                                                                   <  2:4423136/101‑1 (MQ=255)


                               ccGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCg                                                                  <  1:4316136/101‑1 (MQ=255)


                                 gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGc                                                                >  2:4271681/1‑101 (MQ=255)


                                 gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGc                                                                <  2:3027984/101‑1 (MQ=255)


                                  aCGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGcc                                                               <  1:3106297/101‑1 (MQ=255)


                                   cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGccc                                                              >  1:4451238/1‑101 (MQ=255)


                                   cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGccc                                                              >  2:3973148/1‑101 (MQ=255)


                                   cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGccc                                                              >  2:2657609/1‑101 (MQ=255)


                                     gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCGCCCGCCGAGGCGATGCCTCGGccccc                                                            >  2:273703/1‑101 (MQ=255)


                                     gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGccccc                                                            <  2:4320121/101‑1 (MQ=255)


                                      aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGcccccc                                                           >  1:2902630/1‑101 (MQ=255)


                                        gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCgg                                                         >  1:3650312/1‑101 (MQ=255)


                                        gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCgg                                                         <  1:2979110/101‑1 (MQ=255)


                                        gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCgg                                                         >  1:1549032/1‑101 (MQ=255)


                                          aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGa                                                       <  2:3596651/101‑1 (MQ=255)


                                          aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGa                                                       >  1:4340775/1‑101 (MQ=255)


                                           ccGCTTCTGGACTATCTCGAAAGCAAATGCGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGat                                                      <  2:4285185/101‑1 (MQ=255)


                                           ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGat                                                      <  1:2752507/101‑1 (MQ=255)


                                           ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGat                                                      <  1:3324044/101‑1 (MQ=255)


                                                tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                 >  2:3685704/1‑101 (MQ=38)


                                                tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.GGGTGCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                 >  1:3924174/1‑101 (MQ=38)


                                                 cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                >  2:214703/1‑101 (MQ=38)


                                                  tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                               >  2:688882/1‑101 (MQ=38)


                                                   ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                              >  1:1684777/1‑101 (MQ=38)


                                                    gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                             <  1:3035160/101‑1 (MQ=38)


                                                      cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                           >  2:298661/1‑101 (MQ=38)


                                                      cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCGGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                           <  2:2837022/101‑1 (MQ=38)


                                                       tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          <  1:4544268/101‑1 (MQ=38)


                                                       tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          >  2:1515761/1‑101 (MQ=38)


                                                       tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                          >  1:1279395/1‑101 (MQ=38)


                                                        aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                         >  1:1926270/1‑101 (MQ=38)


                                                        aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                         >  1:2835387/1‑101 (MQ=38)


                                                          ctcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGGAATTTTGAGAAAGATg                                       >  2:1756260/1‑101 (MQ=255)


                                                          ctcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                       <  2:4507447/101‑1 (MQ=37)


                                                          ctcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                       <  1:3217946/101‑1 (MQ=37)


                                                           tcGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGa                                      <  2:4311865/101‑1 (MQ=37)


                                                            cGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaa                                     <  1:4217326/101‑1 (MQ=37)


                                                             gAAAGCAAATACGCCGCGATCTCCCGGCTTTGAGGG.C.GAGGGG.GCGCGGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    <  1:3588409/101‑1 (MQ=37)


                                                             gAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                    >  2:1822063/1‑101 (MQ=37)


                                                              aaaGCAAATCCGCCGCGATCTACCGGCTTGGGGGG.CGGG.GGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                   <  1:3570613/101‑1 (MQ=25)


                                                              aaaGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                   >  2:4018677/1‑101 (MQ=37)


                                                               aaGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAgg                                  >  2:2482832/1‑101 (MQ=37)


                                                                aGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                 <  1:3096605/101‑1 (MQ=38)


                                                                aGCAAAGACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                 <  1:792847/101‑1 (MQ=37)


                                                                 gaaaaTGCGCCGCGATTTGCGGGCTTTGAGGG.G.GAGGGG.GCGCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                <  2:4006619/99‑1 (MQ=21)


                                                                 gCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                <  2:4084424/101‑1 (MQ=38)


                                                                 gCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                <  1:1001338/101‑1 (MQ=38)


                                                                  cAAATACGCCGCGATCTCCCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCAt                               <  1:3188004/101‑1 (MQ=37)


                                                                   aaaTACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCTCCCGCCGAGGCGATGCTTCGCCCCCCCGGGACATTTCTAGCAAGATGCAAggaaca                              >  2:158508/1‑97 (MQ=255)


                                                                   aaaTACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                              >  2:1180149/1‑101 (MQ=37)


                                                                   aaaTACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                              >  1:1098638/1‑101 (MQ=37)


                                                                   aaaTACGCCGCGATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                              <  1:4653298/101‑1 (MQ=38)


                                                                      tccgccgcGATCTACCGGCTTTGGGGG.CGGG.GGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                           <  1:927729/99‑1 (MQ=37)


                                                                      caCGCCCCGGTCTACCGGCTTTGAGGG.C.GAGGGG.GGGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                           <  1:522466/100‑1 (MQ=25)


                                                                          ccgcGATCTACCGGCTTGGAGGG.C.GAGGGG.GCCCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                       <  2:1483986/101‑1 (MQ=25)


                                                                             cgATCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGGTTCATc                    >  2:3646701/1‑101 (MQ=37)


                                                                               aTCTTCCGGTTTTGGGGGTG.GG.GGG.GCGCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                  <  1:1756379/101‑1 (MQ=18)


                                                                               aTCTACGGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                  <  2:2982871/101‑1 (MQ=25)


                                                                                tCTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                 >  1:2467835/1‑101 (MQ=25)


                                                                                 cTACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                >  1:1059650/1‑101 (MQ=25)


                                                                                  tACCGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt               >  2:818558/1‑101 (MQ=25)


                                                                                   aCCGGCTTTGGGGG.G.GAGGGG.GCGCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt              <  1:3350482/101‑1 (MQ=21)


                                                                                    ccGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc             <  1:690329/101‑1 (MQ=25)


                                                                                    ccGGCTTTGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc             <  1:3605024/101‑1 (MQ=25)


                                                                                        cTTTGGGGG.G.GAGGGG.GCGCTCCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt         <  2:371845/101‑1 (MQ=21)


                                                                                         tttGGGGG.C.GAGGGG.GCGCGGCGCCCTCCGAGGTGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGcgtttaaga        <  2:1927983/101‑9 (MQ=18)


                                                                                         tttGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa        >  1:3879553/1‑101 (MQ=25)


                                                                                         tttGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa        >  2:2572826/1‑101 (MQ=25)


                                                                                         ttggAGGG.C.GAGGGG.GCGCTTCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa        <  2:3841516/98‑1 (MQ=21)


                                                                                          ttGAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc       >  1:3700862/1‑101 (MQ=25)


                                                                                          gggAGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc       <  1:3444165/99‑1 (MQ=21)


                                                                                           gggggg.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCTCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc      <  1:208558/98‑1 (MQ=21)


                                                                                             aGGG.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc    >  1:58374/1‑101 (MQ=25)


                                                                                               gg.C.GAGGGG.GCGCTGCCCCCGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCgg  <  1:2218101/101‑1 (MQ=25)


CGAGACGATCGTGACCGCCTGCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTTGAGGG.C.GA.GGG.GCGCTGCCCCCCGCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGG  >  NC_014034/1740670‑1740864

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 23 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG