Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,745,454 +C 100% intergenic (‑114/+654) RCAP_rcc01613 ← / ← metH4 hypothetical protein/methionine synthase subunit B

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,745,4541.→C100.0% 54.7 125intergenic (‑114/+654)RCAP_rcc01613/metH4hypothetical protein/methionine synthase subunit B
Reads supporting (aligned to +/- strand):  new base (57/50):  ref base (2/16):  total (59/66)
Fisher's exact test strand distribution p-value = 7.81e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.97e-01

Only 100 of 170 total aligned reads displayed.

GCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CG.GCGCGAGCTTTGGCACCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAGGCGC  >  NC_014034/1745354‑1745549
                                                                                                         |                                                                                               
gCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGGACCT.C.a                                                                                                  >  2:2518424/1‑101 (MQ=255)
 cAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGGCGA.CGCGCGCGCGC.TTGGCACCT.C.A.c                                                                                                >  2:3268094/1‑101 (MQ=255)
   gcgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.ccc                                                                                              <  2:3425423/101‑1 (MQ=255)
    cgcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.cccc                                                                                             >  1:2340661/1‑101 (MQ=255)
     gcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGGTCGGCCAGCGGCCTGGCGATCGCGGCGGTCGA.CGCGCGCGAGC.GTCGCACCT.C.A.ccccc                                                                                            >  1:3166374/1‑101 (MQ=255)
     gcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.ccccc                                                                                            >  2:640293/1‑101 (MQ=255)
     gcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.ccccc                                                                                            >  2:2173161/1‑101 (MQ=255)
     gcCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGCCGATCGCGGCGGACGA.CGCGCGCGAGC.TTGGCACCT.C.A.ccccc                                                                                            >  1:309449/1‑101 (MQ=255)
      ccccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.cccccc                                                                                           <  1:1085989/101‑1 (MQ=255)
       cccTGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGATC.TTGGCACCT.C.A.ccccccc                                                                                          <  2:4299632/101‑1 (MQ=255)
       cccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.ccccccc                                                                                          <  1:3908922/101‑1 (MQ=255)
       cccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.ccccccc                                                                                          <  1:1161050/101‑1 (MQ=255)
        ccGGTGCGGGGGGCGGGGTTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.cccccccc                                                                                         <  2:797688/101‑1 (MQ=255)
        ccGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.cccccccc                                                                                         <  2:195629/101‑1 (MQ=255)
         cGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.ccccccccc                                                                                        >  2:417904/1‑100 (MQ=255)
         cGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.ccccccccc                                                                                        <  2:1047804/101‑2 (MQ=255)
           gTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.cccccccccgg                                                                                      >  1:4615708/1‑98 (MQ=255)
            tggggggggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGTTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.cccccccccggc                                                                                     <  2:714181/98‑5 (MQ=255)
            ggcggggggcgggggTCGGCATCCCGGCCGCCGGACCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCCATC.TTGGAACCT.CAA.CCCCCCCCGgc                                                                                      <  2:634411/100‑1 (MQ=255)
              cggggggcgggggTCGGCAGCCCGGACGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACCC.GCGCGCGC.TTACCACCT.C.A.CCCCCCCCCgcgcc                                                                                   >  2:4478898/1‑100 (MQ=255)
                  gggcgtgggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCg                                                                                <  2:1759800/101‑1 (MQ=255)
                  gggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCg                                                                                <  2:3364963/101‑1 (MQ=255)
                  gggcgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCg                                                                                >  1:2746597/1‑101 (MQ=255)
                     ggggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGca                                                                             <  1:2518598/100‑1 (MQ=255)
                     ggggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGca                                                                             <  1:2708053/100‑1 (MQ=255)
                     cgggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCCCCGGca                                                                             >  1:2554817/1‑101 (MQ=255)
                      gggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGcac                                                                            <  1:4087876/101‑1 (MQ=255)
                       ggggTCGGCCGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGcaca                                                                           <  2:1911893/101‑1 (MQ=255)
                       ggggTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCCGCGCACCGGcaca                                                                           >  2:1723298/1‑101 (MQ=255)
                         ggTCGGCAGCCCGGCCGGCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGc                                                                         <  2:3705026/101‑1 (MQ=255)
                          gTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGcc                                                                        <  1:1376873/101‑1 (MQ=255)
                            cGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAG.GTTGGCACCT.C.ACCCCCCCCCGGCGCGCCGGCACCGCCag                                                                      >  1:4292871/1‑101 (MQ=255)
                               cAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGcccggga                                                                   >  2:1339739/1‑96 (MQ=255)
                                cgCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCagagag                                                                  <  2:922083/100‑1 (MQ=255)
                                 gCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgc                                                                 >  1:4456224/1‑101 (MQ=255)
                                   ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                               <  2:3810614/101‑1 (MQ=255)
                                   ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                               <  2:2598823/101‑1 (MQ=255)
                                   ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                               <  2:2842126/101‑1 (MQ=255)
                                   ccGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAgcgc                                                               <  1:2904473/101‑1 (MQ=255)
                                    cGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCCGCGCACCGGCACAGCCAGAGAGggcg                                                              >  1:2046703/1‑101 (MQ=255)
                                      gCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCgcggc                                                            >  1:358317/1‑101 (MQ=255)
                                          tCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGTGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGcc                                                        <  1:3922258/101‑1 (MQ=255)
                                           cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGACCC.GCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                       >  1:2999060/1‑101 (MQ=255)
                                           cGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCa                                                       <  2:2325382/101‑1 (MQ=255)
                                           cGGATCGGAAAGCGGCATGACGATAGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGGCAGAGGGCGCGGCGGCCa                                                       <  2:4664096/101‑1 (MQ=255)
                                             gATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCaga                                                     >  2:983055/1‑101 (MQ=255)
                                             gATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCaga                                                     >  1:1495082/1‑101 (MQ=255)
                                                cGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                  <  2:686931/101‑1 (MQ=255)
                                                cGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagcc                                                  >  2:3655474/1‑101 (MQ=255)
                                                  gCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                                >  1:1998098/1‑101 (MQ=255)
                                                  gCCAGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGagccag                                                <  1:4463269/101‑1 (MQ=255)
                                                     aGCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGccc                                             <  1:2565051/101‑1 (MQ=255)
                                                      gCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCCCCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGcccc                                            <  1:4530316/101‑1 (MQ=255)
                                                      gCGGCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGcccc                                            <  1:3205123/101‑1 (MQ=255)
                                                        ggCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCCCCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCga                                          >  1:4660156/1‑101 (MQ=255)
                                                         gCATGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCgag                                         <  2:3145495/101‑1 (MQ=255)
                                                           aTGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGagag                                       >  1:3428898/1‑101 (MQ=255)
                                                            tGTCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAgg                                      >  2:463515/1‑101 (MQ=255)
                                                              tCGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGGGAGGGc                                    >  1:1225512/1‑101 (MQ=255)
                                                               cGATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCCGCACACCCCGAGAGGGCGGGGGCAGGACCCCGCCCCGGGGgggag                                   >  2:2089115/1‑99 (MQ=255)
                                                                gATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAg                                  >  2:42282/1‑101 (MQ=255)
                                                                gATCGCGGCGGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCCGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCCCGAGGGCAg                                  >  2:939354/1‑101 (MQ=255)
                                                                  tCGCGGCGGTCGG.CGCGCGCGAGC.TTGCCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTc                                <  1:2821389/101‑1 (MQ=255)
                                                                   cgcgGCGGTCGA.CGCGCGCGAGC.TTGGCCCCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCa                               <  1:2125715/101‑1 (MQ=255)
                                                                    gcggcgGTCGA.CGCGCGCGAGC.TTGGCACCT.C.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAg                             <  1:2371728/101‑1 (MQ=255)
                                                                    gcggcgGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCaa                              >  2:2440512/1‑101 (MQ=255)
                                                                    gcggcgGTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCaa                              >  2:2783964/1‑101 (MQ=255)
                                                                    gcggcgGTCGA.CGCGCGCGAGC.TGGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCaa                              <  1:844948/101‑1 (MQ=255)
                                                                     cggcggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGGACAACCAGAAAGCACGCCGGCCAGCACCAGCCCCGAGAGGGCTGTCAAg                             >  1:3822779/1‑101 (MQ=255)
                                                                     cggcggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAg                             >  1:1276580/1‑101 (MQ=255)
                                                                     cggcggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAg                             <  2:3859417/101‑1 (MQ=255)
                                                                      ggcggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAgg                            >  2:3685979/1‑101 (MQ=255)
                                                                      ggcggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAgg                            <  1:1009971/101‑1 (MQ=255)
                                                                      ggcggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAgg                            <  1:2963606/101‑1 (MQ=255)
                                                                      ggcggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAgg                            <  2:2998831/101‑1 (MQ=255)
                                                                      ggcggTCGA.CGCGCGCGAGC.TTGGCACCT.C.A.CCCCCCCCCGCGCCCCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCCGCCCCCAGGGGGCAGGCAAGGa                           >  1:3715849/1‑101 (MQ=255)
                                                                        cggTCGC.CGCGCGGGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGaa                          <  2:3497700/101‑1 (MQ=255)
                                                                        cggTCGA.CGCGCGCGAGC.TTGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGaa                          <  1:1339832/101‑1 (MQ=255)
                                                                         ggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCCCCCGCACAGCCAGAGAGCGCGGCGGCCAGAGCCCGCCCCGAGAGGGCAGTCAAGGAAc                         >  1:282684/1‑101 (MQ=255)
                                                                         ggTCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAc                         <  2:1196227/101‑1 (MQ=255)
                                                                           tCGA.CGCGCGCGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcgg                       <  1:4444528/101‑1 (MQ=255)
                                                                             cgacgcgcgcGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggc                      >  2:3321248/7‑101 (MQ=255)
                                                                             cgacgcgcgcGAGC.TTGGCACCT.C.ACCCCCCCCCCGCGCACCGGCAACGCCAGAAAGCGCGGCGGCCAAAGCCAGCCCCGAGAGGGCAGCCAAGGAAaggc                      >  1:2190554/7‑101 (MQ=255)
                                                                              gacgcgcgcGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggcg                     >  2:1712011/6‑101 (MQ=255)
                                                                              gacgcgcgcGAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAcggcg                     <  2:1781571/96‑1 (MQ=255)
                                                                                   gcgcGAGC.TTGGCGCCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGccc                <  2:2412612/101‑1 (MQ=255)
                                                                                    ccgcggtgttGGCCCCT.C.CCCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGccc                <  2:3025775/93‑1 (MQ=255)
                                                                                     gcgAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGgccca              >  2:2636674/1‑99 (MQ=255)
                                                                                     cgcgggcttGGCCCCT.C.CCCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCg               <  2:2483103/94‑1 (MQ=255)
                                                                                      cgAGC.TTGGCCCCT.C.CCCCCCCCCCGGCGCACCCGCCCAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGaa             <  1:3655742/101‑1 (MQ=255)
                                                                                      cgAGC.TTGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCCaa             >  2:1811721/1‑101 (MQ=255)
                                                                                         agcttGGCACCTCC.C.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgc           <  1:1723409/98‑1 (MQ=255)
                                                                                         agcttGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGGGAGCGCGGCGGCCAGAGCCAGCCCCCAGAGGGCAGTCAAGGAACGGCGGGCCCGCAgg           >  1:4222477/4‑100 (MQ=255)
                                                                                         agcttGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAAAGGCGGGCCCGAAgc           >  2:3090460/4‑101 (MQ=255)
                                                                                          gcttGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGGAGGCAAGGAACGGCGGGCCCCaaccc          >  2:1231846/3‑98 (MQ=255)
                                                                                          gcttGGCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCCCAgcg          >  1:3603666/3‑101 (MQ=255)
                                                                                           cttGGCCCCT.C.CCCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAgcgc         <  1:4059940/100‑1 (MQ=255)
                                                                                             tGGCACCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGCAGCGCacg      >  1:3557414/1‑99 (MQ=255)
                                                                                              ggCACCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGgccccaacgccag      >  2:2523639/1‑91 (MQ=255)
                                                                                                 aCCT.C.ACCCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCCGCCCCGAGAGGGCAGTCAAGGAACGGCGGGGCCCGCGCGCCAGgcg   >  1:3260243/1‑101 (MQ=255)
                                                                                                         |                                                                                               
GCAGCGCCCCGGTGCGGGGGGCGGGGGTCGGCAGCCCGGCCGTCGGATCGGCCAGCGGCATGTCGATCGCGGCGGTCGA.CG.GCGCGAGCTTTGGCACCT.C.A.CCCCCCCCGGCGCACCGGCACAGCCAGAGAGCGCGGCGGCCAGAGCCAGCCCCGAGAGGGCAGTCAAGGAACGGCGGGCCCGAAGCGCAAGGCGC  >  NC_014034/1745354‑1745549

Base quality scores:  ATCG  < 3 ≤  ATCG  < 13 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG