Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,370 +G 92.9% coding (442/744 nt) RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3701.→G92.9% 27.9 ‑4.5 ‑0.0154coding (442/744 nt)RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (70/73):  ref base (1/10):  total (71/83)
Fisher's exact test strand distribution p-value = 1.14e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.38e-01

Only 100 of 172 total aligned reads displayed.

TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462
                                                                                                     |                                                                                              
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTc                                                                                                 >  1:3537589/1‑101 (MQ=255)
tgtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTc                                                                                                 >  1:2695211/1‑101 (MQ=255)
 gtgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcg                                                                                                <  1:345907/101‑2 (MQ=255)
  tgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgc                                                                                               <  2:3119974/101‑3 (MQ=255)
  tgCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgc                                                                                               >  1:2647654/1‑99 (MQ=255)
   gCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgcg                                                                                              <  2:801508/101‑4 (MQ=255)
     ggCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgcgcg                                                                                            >  2:4598198/1‑96 (MQ=255)
     ggCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgcgcg                                                                                            <  2:1704305/101‑6 (MQ=255)
     ggCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgcgcg                                                                                            >  1:3701188/1‑96 (MQ=255)
          cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgcgcgcgggc                                                                                       <  2:2172477/101‑11 (MQ=255)
          cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGGGGTcgcgcgcgggc                                                                                       >  1:13508/1‑91 (MQ=255)
          cGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAAACGTCGCGGCTTTGGGGGCGGTCGATGTCGGGCAGGGGGCGGTGGTcgcgcgcgggc                                                                                       >  2:4376425/1‑91 (MQ=255)
            tCGGCGATGAGGACCAAATGGATGCGTCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCtt                                                                                   <  1:1413095/101‑1 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                  <  2:6641/101‑1 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                  <  2:508630/101‑1 (MQ=255)
             cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCttt                                                                                  <  2:1221806/101‑1 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                                             <  1:1042114/101‑1 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCtt                                                                             >  2:4090410/1‑101 (MQ=255)
                  aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGGGGTCGCG.CG.CGGGCTTTGCCtt                                                                             >  1:2927004/1‑101 (MQ=255)
                   ttaGGACCATCTGTATGCGGCCCGCGCCGCCGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                            <  2:1532752/99‑1 (MQ=255)
                   tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTg                                                                            <  1:1448765/101‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGGTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           <  1:2308698/101‑1 (MQ=255)
                    cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:1770510/1‑101 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                          <  1:2367030/101‑1 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                          >  2:4157532/1‑101 (MQ=255)
                     aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTggg                                                                          >  2:2821891/1‑101 (MQ=255)
                        aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTc                                                                       >  2:1854549/1‑101 (MQ=255)
                         ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:546470/101‑1 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:600059/101‑1 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:2336117/101‑1 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     >  2:3660096/1‑101 (MQ=255)
                          cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:3183989/101‑1 (MQ=255)
                             cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  >  2:1890276/1‑101 (MQ=255)
                             cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  <  2:2926775/101‑1 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:3504968/101‑1 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  2:1935408/101‑1 (MQ=255)
                                 aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:2822107/101‑1 (MQ=255)
                                  tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                             <  1:4554001/101‑1 (MQ=255)
                                    cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           >  1:452902/1‑101 (MQ=255)
                                    cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           >  1:24753/1‑101 (MQ=255)
                                     ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          >  2:4352100/1‑101 (MQ=255)
                                      gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCCTCCggg                                                         >  2:2329502/1‑101 (MQ=255)
                                       cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        >  1:1712729/1‑101 (MQ=255)
                                       cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  2:13503/101‑1 (MQ=255)
                                           cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  1:1856763/101‑1 (MQ=255)
                                           cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGGCGAGGCGCTTCCGGGCACCg                                                    >  1:4504822/1‑101 (MQ=255)
                                           cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTC.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  2:401178/101‑1 (MQ=255)
                                             cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  >  2:1268840/1‑101 (MQ=255)
                                                cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               <  2:2819319/101‑1 (MQ=255)
                                                cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGGGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               >  1:1927771/1‑101 (MQ=255)
                                                 ggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              <  2:4435553/101‑1 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  1:1507826/1‑101 (MQ=255)
                                                  gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             <  2:4591319/101‑1 (MQ=255)
                                                    aaTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           >  1:4047495/1‑101 (MQ=255)
                                                      tcgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                         <  1:4482538/101‑1 (MQ=255)
                                                      tcgtcgAGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                         <  1:754255/101‑1 (MQ=255)
                                                        gtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       >  1:2800634/1‑101 (MQ=255)
                                                          cgcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  1:1285487/1‑101 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CTGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGTCACCGATGCGCTCCTTGAttt                                    <  2:4165965/101‑1 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    >  1:1147029/1‑101 (MQ=255)
                                                           gcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:1203926/101‑1 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   >  1:3635515/1‑101 (MQ=255)
                                                            cgGCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  2:1870757/101‑1 (MQ=255)
                                                             ggCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  1:2670109/1‑101 (MQ=255)
                                                              gCTTTGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgt                                 <  2:3700913/101‑1 (MQ=255)
                                                                tttGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               <  1:2028947/101‑1 (MQ=255)
                                                                tttGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  2:1725644/1‑101 (MQ=255)
                                                                tttGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  2:1129118/1‑101 (MQ=255)
                                                                  tGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  2:2151035/1‑101 (MQ=255)
                                                                  tGGGCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  2:3890954/1‑101 (MQ=255)
                                                                   gggCGCGGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccg                            <  2:586940/101‑1 (MQ=255)
                                                                      cgcgGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCgcgc                         >  2:2720954/1‑101 (MQ=255)
                                                                        cgGTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       >  1:3880384/1‑101 (MQ=255)
                                                                         ggTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:2669924/101‑1 (MQ=255)
                                                                         ggTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:1067852/101‑1 (MQ=255)
                                                                         ggTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      >  1:933243/1‑101 (MQ=255)
                                                                          gTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     <  1:316542/101‑1 (MQ=255)
                                                                          gTCGATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     <  2:2475359/101‑1 (MQ=255)
                                                                             gATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  1:3891831/1‑101 (MQ=255)
                                                                             gATGTCGGGCAGGGGGCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  2:1293090/1‑101 (MQ=255)
                                                                               tgtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGc                <  1:1814871/91‑1 (MQ=255)
                                                                                gtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               >  2:2587093/10‑101 (MQ=255)
                                                                                gtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               <  2:2631914/92‑1 (MQ=255)
                                                                                gtcgggcagggggCGGTGGTCGCG.CG.CGGGCTTGGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCt               <  1:4018433/92‑1 (MQ=255)
                                                                                 tcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  2:3013847/93‑1 (MQ=255)
                                                                                 tcgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  2:4211608/93‑1 (MQ=255)
                                                                                  cgggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCCTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTgg             >  1:4163124/8‑101 (MQ=255)
                                                                                   gggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            <  2:3537331/95‑1 (MQ=255)
                                                                                    ggcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtg           <  1:3768968/96‑1 (MQ=255)
                                                                                     gcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  1:4090696/97‑1 (MQ=255)
                                                                                     gcagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  1:3808386/97‑1 (MQ=255)
                                                                                      cagggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  1:507925/4‑101 (MQ=255)
                                                                                       agggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        >  2:3333363/3‑101 (MQ=255)
                                                                                        gggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       >  2:1172049/2‑101 (MQ=255)
                                                                                         ggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGTCTGGtgccgat      <  2:3869126/101‑1 (MQ=255)
                                                                                         ggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  1:4352392/101‑1 (MQ=255)
                                                                                         ggggCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  1:624628/101‑1 (MQ=255)
                                                                                         ggggCGGTGGTC.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      >  1:1319554/1‑101 (MQ=255)
                                                                                            gCGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   <  2:452865/101‑1 (MQ=255)
                                                                                             cGGTGGTCGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  >  2:487192/1‑101 (MQ=255)
                                                                                                     |                                                                                              
TGTGCGGCAGCGTCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGGGCGGTGGTC.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769272‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG