Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,808,662 +T 97.6% intergenic (‑68/‑223) RCAP_rct00023 ← / → RCAP_rcc01670 tRNA‑Leu/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,808,6621.→T97.6% 7.1 ‑1.0 ‑1.8206intergenic (‑68/‑223)RCAP_rct00023/RCAP_rcc01670tRNA‑Leu/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (99/101):  ref base (4/1):  total (104/102)
Fisher's exact test strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.62e-02

Only 100 of 236 total aligned reads displayed.

CTGGTGCGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GG.CCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.TTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCGGCAC  >  NC_014034/1808563‑1808759
                                                                                                        |                                                                                                 
  ggTGCGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.tt                                                                                                 <  1:1425200/101‑1 (MQ=255)
   gTGCGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TT.GGG.ttt                                                                                                >  2:4579818/1‑101 (MQ=255)
   gTGCGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttt                                                                                                <  1:572169/101‑1 (MQ=255)
    tGCGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.tttt                                                                                               >  1:320364/1‑100 (MQ=255)
    tGCGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.tttt                                                                                               >  1:1935108/1‑100 (MQ=255)
     gCGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttg                                                                                              <  2:1184363/101‑3 (MQ=255)
      cGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttgt                                                                                             >  1:2918487/1‑98 (MQ=255)
      cGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttgt                                                                                             <  1:4596088/101‑4 (MQ=255)
      cGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttgt                                                                                             <  2:2497650/101‑4 (MQ=255)
      cGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttgt                                                                                             <  2:3325757/101‑4 (MQ=255)
      cGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttgt                                                                                             >  2:754947/1‑98 (MQ=255)
       gTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttgtt                                                                                            <  1:682518/101‑5 (MQ=255)
       gTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttgtt                                                                                            >  1:4546926/1‑97 (MQ=255)
        tCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.ttttgtta                                                                                           >  1:3482237/1‑96 (MQ=255)
         cTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTaa                                                                                           >  2:95883/1‑101 (MQ=255)
          tACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGTGGGCAAAA.TA.GGGTTTTGTTAAc                                                                                          >  1:2489216/1‑101 (MQ=255)
          tACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAATTG.GGG.TTTGTTAAc                                                                                          >  2:3491729/1‑101 (MQ=255)
          tACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAAc                                                                                          <  2:3673354/101‑1 (MQ=255)
          tACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAAc                                                                                          <  2:3762720/101‑1 (MQ=255)
             cAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCtt                                                                                       >  1:3142146/1‑101 (MQ=255)
             cAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCtt                                                                                       <  1:1112867/101‑1 (MQ=255)
              aGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCttt                                                                                      <  1:3636376/101‑1 (MQ=255)
               gTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTc                                                                                     <  2:1444971/101‑1 (MQ=255)
                tttCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTcc                                                                                    >  2:578141/1‑101 (MQ=255)
                tttCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTcc                                                                                    <  2:3395847/101‑1 (MQ=255)
                tttCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTcc                                                                                    >  1:499036/1‑101 (MQ=255)
                   cGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCt                                                                                 >  1:963599/1‑101 (MQ=255)
                    gCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTc                                                                                >  2:2161601/1‑101 (MQ=255)
                     ccACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCg                                                                               <  2:2114380/101‑1 (MQ=255)
                        cacCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCa                                                                            <  1:4341199/101‑1 (MQ=255)
                         ccccGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCag                                                                           <  2:2851927/100‑1 (MQ=255)
                         acCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCag                                                                           <  2:2575712/101‑1 (MQ=255)
                           ccGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCagag                                                                         >  1:1251669/1‑101 (MQ=255)
                            cGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAgc                                                                        >  1:2783138/1‑101 (MQ=255)
                            cGCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGATTTGTTAACCTTTCCCCTCGCCAGAgc                                                                        >  1:3087523/1‑101 (MQ=255)
                             gCACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAgcg                                                                       <  1:2447874/101‑1 (MQ=255)
                              cACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTGGGGGGGCAACAGTTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAgcgc                                                                      >  1:3834603/1‑101 (MQ=255)
                              cACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAgcgc                                                                      >  2:3272318/1‑101 (MQ=255)
                              cACTGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAgcgc                                                                      >  1:269353/1‑101 (MQ=255)
                                 tGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCggg                                                                   <  1:2544999/101‑1 (MQ=255)
                                 tGCCCTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCggg                                                                   >  2:4040516/1‑101 (MQ=255)
                                  gCCCTCGCTTTTAGCAACTGGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCGAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCgggg                                                                  >  2:742518/1‑101 (MQ=255)
                                    ccTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGcc                                                                >  2:1363197/1‑101 (MQ=255)
                                    ccTCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGcc                                                                >  1:828600/1‑101 (MQ=255)
                                      tCGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTg                                                              <  2:3936054/101‑1 (MQ=255)
                                       cGCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGt                                                             >  1:4444288/1‑101 (MQ=255)
                                        gCTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTc                                                            >  2:1786895/1‑101 (MQ=255)
                                         cTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTcc                                                           >  2:1156216/1‑101 (MQ=255)
                                         cTTTTAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTcc                                                           >  1:2016257/1‑101 (MQ=255)
                                          ttttAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCt                                                          <  2:4443993/101‑1 (MQ=255)
                                            ttAGCAACTCGCGCAGC.CCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAg                                                        >  2:2349650/1‑101 (MQ=255)
                                            ttAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCGCTCGCCAGAGCGCGGGGCCTGTCCTAg                                                        >  2:1935584/1‑101 (MQ=255)
                                            ttAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAg                                                        >  2:2220542/1‑101 (MQ=255)
                                            ttAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAg                                                        <  2:1531827/101‑1 (MQ=255)
                                             tAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAgg                                                       <  1:934455/101‑1 (MQ=255)
                                             tAGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAgg                                                       <  2:277652/101‑1 (MQ=255)
                                              aGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGc                                                      >  2:4122002/1‑101 (MQ=255)
                                              aGCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGc                                                      >  1:2109871/1‑101 (MQ=255)
                                               gCAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCt                                                     <  1:2977981/101‑1 (MQ=255)
                                                cAACTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCtt                                                    >  2:1749152/1‑101 (MQ=255)
                                                 aaCTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTc                                                   <  1:1711178/101‑1 (MQ=255)
                                                  aCTCGCGC.GGCCCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTcc                                                  <  1:2471030/101‑1 (MQ=255)
                                                         gcggccccTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCag                                            <  2:86695/96‑1 (MQ=255)
                                                         gcggccccTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCag                                            <  1:797437/96‑1 (MQ=255)
                                                              cccTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCg                                       <  1:3775923/101‑1 (MQ=255)
                                                               ccTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGc                                      >  1:2335762/1‑101 (MQ=255)
                                                                cTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCa                                     >  2:2279368/1‑101 (MQ=255)
                                                                 tgtgTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCaa                                    >  1:4079919/1‑101 (MQ=255)
                                                                   tgtAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAg                                  >  1:1538007/1‑101 (MQ=255)
                                                                      aGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGcc                               <  1:229684/101‑1 (MQ=255)
                                                                      aGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGcc                               >  2:2669993/1‑101 (MQ=255)
                                                                         ggggCAACAGGGCTGCGGGCAAAA.TAGGGG.TTTTTTTACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGGTCAGAGCCGCAAAgggccga                            >  2:4479922/1‑96 (MQ=255)
                                                                           ggCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTTTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAgatg                          <  2:4387729/101‑1 (MQ=255)
                                                                           ggCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAgatg                          >  2:431383/1‑101 (MQ=255)
                                                                            gCAACAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAgatga                         <  2:304918/101‑1 (MQ=255)
                                                                              aaCAGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATc                       >  2:3500092/1‑101 (MQ=255)
                                                                                 aGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCaaa                    <  1:870592/101‑1 (MQ=255)
                                                                                 aGGTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCaaa                    <  1:2432463/101‑1 (MQ=255)
                                                                                  ggTCTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCaaaa                   <  1:4060777/101‑1 (MQ=255)
                                                                                     cTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAg                >  1:4252818/1‑101 (MQ=255)
                                                                                     cTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAg                >  1:2144217/1‑101 (MQ=255)
                                                                                     cTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAg                <  1:1605645/101‑1 (MQ=255)
                                                                                     cTGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAg                <  1:323049/101‑1 (MQ=255)
                                                                                      tGCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGc               >  1:2901675/1‑101 (MQ=255)
                                                                                       gCGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGcc              <  2:3104428/101‑1 (MQ=255)
                                                                                        cGGGCAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCg             >  2:2022852/1‑101 (MQ=255)
                                                                                            cAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGaaa         >  2:1008345/1‑101 (MQ=255)
                                                                                            cAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGaaa         >  2:1897199/1‑101 (MQ=255)
                                                                                            cAAAA.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGaaa         >  2:3833097/1‑101 (MQ=255)
                                                                                              aaa.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAAcc       <  1:1749271/101‑1 (MQ=255)
                                                                                               aa.TA.GGGTTTTTTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGTTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCg      <  1:1174415/101‑1 (MQ=255)
                                                                                               aa.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCg      <  2:1727703/101‑1 (MQ=255)
                                                                                               aa.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCg      <  1:2944279/101‑1 (MQ=255)
                                                                                               aa.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCg      >  1:4519925/1‑101 (MQ=255)
                                                                                               aa.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCg      <  2:3427035/101‑1 (MQ=255)
                                                                                                a.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCgg     <  2:2937332/101‑1 (MQ=255)
                                                                                                a.TA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCgg     >  2:808261/1‑101 (MQ=255)
                                                                                                  tA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCGGc    >  2:4374598/1‑101 (MQ=255)
                                                                                                  tA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCGGc    <  1:311951/101‑1 (MQ=255)
                                                                                                  tA.GGGTTTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCGGc    <  2:4546631/101‑1 (MQ=255)
                                                                                                        |                                                                                                 
CTGGTGCGTCTACCAGTTTCGCCACACCCGCACTGCCCTCGCTTTTAGCAACTCGCGC.GG.CCCTGTGTAGGGGGGCAACAGGTCTGCGGGCAAAA.TA.GGG.TTTGTTAACCTTTCCCCTCGCCAGAGCGCGGGGCCTGTCCTAGGCTTCCGATCAGAGCCGCAAAGGCCAGATGATCAAAAAAGCCGGAAACCGGCAC  >  NC_014034/1808563‑1808759

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG