Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,813,101	+G	95.7%	intergenic (+145/‑73)	glnA3 → / → fabD	glutamine synthetase/ACP S‑malonyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,813,101	1	.→G	95.7%	2.6 ‑2.1 ‑1.0	69	intergenic (+145/‑73)	glnA3/fabD	glutamine synthetase/ACP S‑malonyltransferase
Reads supporting (aligned to +/- strand):  new base (64/2):  ref base (2/1):  total (66/3)
Fisher's exact test strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.09e-01

TTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTT  >  NC_014034/1813001‑1813193                                                                                                       |                                                                                             ttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaa                                                                                               <  1:1119402/101‑1 (MQ=255) ttttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaa                                                                                               <  1:718524/101‑1 (MQ=255)  tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                                              >  2:4667374/1‑100 (MQ=255)  tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                                              <  2:3487223/101‑2 (MQ=255)  tttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaag                                                                                              >  1:3563765/1‑100 (MQ=255)   ttGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagc                                                                                             <  1:3480193/101‑3 (MQ=255)       gggggTGCGACTGCGACTCTTTGCGTATTTGAGCAAAAAAAAACCCCGGCGTTTTTTCTTGGTT.AAAATACGCCGGGGGGGTCCGGGGGCGCGAA.CCcccc                                                                                        <  2:2821557/100‑2 (MQ=25)       cGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagccccc                                                                                         >  2:2212511/1‑95 (MQ=38)       cGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagccccc                                                                                         >  1:1189089/1‑95 (MQ=38)       cGGGGTCCGACTGCGACTCTTGGCGTATTTGAGCCAAGAAAAACCCCGGCGTTTTTTCTTGGTT.AAAATCCGCCGGGGGGGTCGGGGGGCGCGAA.CCcccc                                                                                        <  2:2876162/101‑2 (MQ=25)        ggggTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGaagcccccg                                                                                        >  2:2409550/1‑94 (MQ=38)            tgcgactgcgacTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgc                                                                                    >  2:3627552/1‑100 (MQ=37)               gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                 >  2:4129333/1‑97 (MQ=25)               gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                 >  1:4398759/1‑97 (MQ=25)               gactgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCcggcgcttc                                                                                 >  1:1336451/1‑97 (MQ=25)                actgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTcc                                                                               >  2:564065/1‑101 (MQ=25)                  tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                             >  2:4214237/1‑101 (MQ=25)                  tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                             >  1:1144240/1‑101 (MQ=25)                  tgcgactCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCgg                                                                             >  2:801685/1‑101 (MQ=25)                       ctttttGCGTATTTGAGCCAAAAAAAACCCCGGCGTTTTTTCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                        <  2:2988887/98‑1 (MQ=16)                       ctctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCt                                                                        >  1:1933250/1‑101 (MQ=25)                        tctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTc                                                                       >  2:3747225/1‑101 (MQ=25)                         ctTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTcc                                                                      >  1:1502206/1‑101 (MQ=25)                          tttGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCg                                                                     >  2:3086521/1‑101 (MQ=25)                             gCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGAc                                                                  >  2:838749/1‑101 (MQ=25)                                tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                               >  2:2486626/1‑101 (MQ=25)                                tATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCt                                                               >  2:192103/1‑101 (MQ=25)                                 aTTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTg                                                              >  2:915709/1‑101 (MQ=25)                                  tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAA.GCCCCCGGAGCTTCCGGTTGCTCCGGACGCTGc                                                             >  2:4663471/1‑101 (MQ=37)                                  tttGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGGCAGGGGGCGCCAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGc                                                             >  2:3647550/1‑101 (MQ=25)                                    tGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGt                                                           >  1:173575/1‑101 (MQ=25)                                     gAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTc                                                          >  2:2315396/1‑101 (MQ=37)                                      aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                                         >  1:1162640/1‑101 (MQ=25)                                      aGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCa                                                         >  1:4393769/1‑101 (MQ=25)                                       gCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCaa                                                        >  1:835443/1‑101 (MQ=37)                                         cAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGt                                                      >  2:250587/1‑101 (MQ=37)                                          aaGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGtt                                                     >  2:1954501/1‑101 (MQ=25)                                           aGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTa                                                    >  2:1973219/1‑101 (MQ=25)                                              aaaacCCCGGGGTTTTTTTTTGGTTAAAAACCCGCGGGGGGGGCCGGGGGGCGAGAC.CCCCCGGGCGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTaga                                                  <  1:2078877/101‑1 (MQ=255)                                               aaaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAgaga                                                >  1:17878/1‑101 (MQ=25)                                                aaaCCCGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGaa                                               >  1:3557810/1‑101 (MQ=25)                                                   cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                            >  2:4389417/1‑101 (MQ=25)                                                   cccGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGc                                            >  1:3338108/1‑101 (MQ=25)                                                     cggctttttttCTTGGTT.CAAATCCGCCGGGGGGGTCGGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                                          <  2:3792142/95‑1 (MQ=21)                                                     cGGCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCa                                          >  2:1598908/1‑101 (MQ=25)                                                      ggCGCTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAg                                         >  1:824874/1‑101 (MQ=25)                                                          cTTTTTCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGAttt                                     >  1:632617/1‑101 (MQ=25)                                                             tttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTc                                  >  1:1592523/1‑101 (MQ=25)                                                              ttCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCa                                 <  2:1502097/101‑1 (MQ=25)                                                               tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                                >  2:622905/1‑101 (MQ=25)                                                               tCTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCaa                                >  2:2160954/1‑101 (MQ=25)                                                                cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                               >  1:2921366/1‑101 (MQ=25)                                                                cTTGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAag                               >  2:4121127/1‑101 (MQ=25)                                                                 ttGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagg                              >  2:976548/1‑101 (MQ=25)                                                                  tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagga                             >  2:3936702/1‑101 (MQ=25)                                                                  tGGTT.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAagaa                             >  2:4313579/1‑99 (MQ=25)                                                                     tt.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGc                          >  2:4332921/1‑101 (MQ=25)                                                                      t.CAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCg                         >  1:4117785/1‑101 (MQ=25)                                                                        cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                        >  2:3714787/1‑101 (MQ=25)                                                                        cAAATACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCgg                        >  2:1616873/1‑101 (MQ=25)                                                                          aaTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGc                      >  2:2777908/1‑101 (MQ=37)                                                                           aTACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCa                     >  2:3059868/1‑101 (MQ=37)                                                                           aTACGCCGCGGGGGCCCGGGGGGGCGAAGCCCCCGGC.CCTTCCGGTTGCCGCGGACGCCGCGGCAAGTTAGAGAAGGCCCGATTCGCCAAGGCGGCgggga                     >  2:4162112/1‑99 (MQ=255)                                                                            tACGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCAt                    >  2:2337833/1‑101 (MQ=37)                                                                             aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg                   >  2:2441444/1‑101 (MQ=37)                                                                             aCGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATg                   >  2:4324890/1‑101 (MQ=37)                                                                              cGCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGa                  >  2:4362670/1‑101 (MQ=37)                                                                               gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGNCNNGNg                 >  1:789149/1‑101 (MQ=25)                                                                               gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg                 >  2:4143351/1‑101 (MQ=37)                                                                               gCCGCGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAg                 >  1:1724759/1‑101 (MQ=37)                                                                                 cgcGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCg               >  1:2156774/3‑101 (MQ=37)                                                                                   cGGGGGTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTg             >  1:3756273/1‑101 (MQ=38)                                                                                    gggggTCTGGGGGCGCGAA.GCCCCCGGGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGCTTGAGAAGGCCAGATTTCTCAAGGGGGCGGGCATGAGCGTgg            >  2:1219808/1‑101 (MQ=255)                                                                                    gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg            >  2:4172337/1‑101 (MQ=38)                                                                                    gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg            >  2:1541399/1‑101 (MQ=38)                                                                                    gggggTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTgg            >  1:539481/1‑101 (MQ=38)                                                                                        gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg        >  2:795242/1‑101 (MQ=38)                                                                                        gTCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATg        >  1:2251609/1‑101 (MQ=38)                                                                                         tCCGGGGGCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATgg       >  2:3252704/1‑101 (MQ=255)                                                                                           cgggggCGCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCCTGGgt     >  2:2438943/1‑101 (MQ=255)                                                                                            gggggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg    >  1:4261984/4‑101 (MQ=255)                                                                                            gggggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGgtg    >  1:4232699/4‑101 (MQ=255)                                                                                              gggcgCGAAGCCCCCGGC.GCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGTATGGGTGtt  >  1:2395688/2‑101 (MQ=255)                                                                                                       |                                                                                             TTTTGCCGGGGTGCGACTGCGACTCTTTGCGTATTTGAGCCAAGAAAAAACCCGGCGCTTTTTCTTGGTT.CAAATACGCCTCGGGGGTCCGGCGGCGCGAA.CGCCCGGCGGCTTCCGGTTGCTCCGGACGCTGCGTCAAGTTAGAGAAGGCCAGATTTGTCAAGGAGGCGGGCATGAGCGTGGCATGGGTGTT  >  NC_014034/1813001‑1813193 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG