Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,843,420 +C 100% intergenic (+82/+66) ppiB → / ← tyrS peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,843,4201.→C100.0% 473.9 150intergenic (+82/+66)ppiB/tyrSpeptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (87/63):  ref base (0/0):  total (87/63)

Only 100 of 161 total aligned reads displayed.

AAGGCCACGATCGAGTGACACGGGCGCA.GGGG.GCTCTGCCCCCCCTCT.G.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.G.TCCGCT.GG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.GG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCG  >  NC_014034/1843321‑1843512
                                                                                                         |                                                                                                  
aaGGCCGCGATCGAGTGACACGGGCGCA.GGGGCGCTTTG.GCCCCCTCTGG.TGCGGCTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.g                                                                                                     <  2:3790504/101‑1 (MQ=25)
    ccACGATCGAGTGACACGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.gctcc                                                                                                 <  2:4619031/101‑5 (MQ=25)
        gATCGAGTGACACGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.g                                                                                            <  1:3637734/101‑1 (MQ=25)
        gATCGAGTGACACGGGCGCAGGGGG.GCTCTG.CCACCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.g                                                                                            >  2:1599778/1‑101 (MQ=21)
          tCGAGTGACACGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.g                                                                                         <  1:4039596/101‑1 (MQ=25)
             aGTGACACGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggc                                                                                      <  1:3682779/101‑3 (MQ=21)
             aGTGACACGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggc                                                                                      <  1:3708239/101‑3 (MQ=21)
             aGTGACACGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggc                                                                                      <  1:3756269/101‑3 (MQ=21)
             aGTGACACGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggc                                                                                      <  2:3787784/101‑3 (MQ=21)
               tGACACGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggcgc                                                                                    <  2:4089810/101‑5 (MQ=21)
                gACACGGGCGCAGGGGG.GCTCTGCCCCCCC.CT.GCTGCGCATTCACCCCCCGGGAGATTTTCGCCCAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggcgcc                                                                                   >  2:2722341/1‑96 (MQ=21)
                   acGGGCGCAGGGGG.GCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCtt                                                                                 <  1:3290981/101‑1 (MQ=21)
                        cgcaggggg.gCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATc                                                                            <  2:2496662/96‑1 (MQ=21)
                        cgcaggggg.gCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATc                                                                            <  2:4546926/96‑1 (MQ=21)
                          caggggg.gCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCtt                                                                          <  1:3470676/98‑1 (MQ=21)
                           aggggg.gCTCTGCCCCCTCT.G.C.TGCGCCTTCACCCCCCCCGCCATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGc                                                                        >  2:2043596/3‑101 (MQ=255)
                           aggggg.gCTCTG.CCCCCCTCT.GCTGCGCATTCGCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTg                                                                         <  2:1218018/99‑1 (MQ=21)
                             gggg.gCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGcc                                                                       >  1:3437789/1‑101 (MQ=21)
                                  gCTCTG.CCCCCCTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACaa                                                                   >  1:170574/1‑101 (MQ=25)
                                     tctgccccccTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCCTCTTGCCACAAat                                                                 >  2:540449/5‑101 (MQ=25)
                                     tctgccccccTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAat                                                                 >  1:715393/5‑101 (MQ=25)
                                     tctgccccccTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAat                                                                 >  2:1630873/5‑101 (MQ=25)
                                     tctgccccccTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAat                                                                 <  1:4551485/97‑1 (MQ=25)
                                        gccccccTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATc                                                              >  1:2297257/2‑101 (MQ=37)
                                          cccccTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATccc                                                            >  2:120312/1‑101 (MQ=37)
                                          cccccTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATccc                                                            <  2:3979555/101‑1 (MQ=37)
                                             ccTCT.GCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.ggg                                                        <  1:3739770/101‑1 (MQ=37)
                                               ctctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.gggg                                                       >  1:2965699/7‑101 (MQ=37)
                                                tctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.t                                                     >  2:3099846/6‑101 (MQ=38)
                                                 ctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCA.GGGC.Tc                                                    >  1:768566/5‑101 (MQ=37)
                                                  tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tcc                                                   <  1:4466189/98‑1 (MQ=38)
                                                  tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tcc                                                   >  1:1330472/4‑101 (MQ=38)
                                                  tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tcc                                                   >  2:35624/4‑101 (MQ=38)
                                                   gcggggcATTCTCCCCCAGGGATATTTTCGCCAAGGTGAAATGGAAAACGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.g                                                 <  2:1171744/96‑1 (MQ=25)
                                                    ctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg                                                >  2:4155867/2‑101 (MQ=38)
                                                     tgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.g                                              >  1:3895564/1‑101 (MQ=38)
                                                      gcgcATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.gg                                             <  2:2373885/101‑2 (MQ=38)
                                                       cgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggc                                            >  2:1731991/1‑99 (MQ=38)
                                                       cgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggc                                            <  1:2625026/101‑3 (MQ=38)
                                                        gcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggca                                           <  2:1354266/101‑4 (MQ=38)
                                                         cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcag                                          >  2:4171855/1‑97 (MQ=37)
                                                         cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcag                                          >  1:3290351/1‑97 (MQ=37)
                                                          aTTCACCCCCGGTGATATTTTGGCCAAGGCGAAATGGCAAAAGGC.GCTCCGCT.GGAGGCGCCCTTTCATCTTGCCACAAATATCTA.GGGG.TCC.gg.gacaga                                         >  2:2876788/1‑96 (MQ=19)
                                                          aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcaga                                         >  1:347602/1‑96 (MQ=37)
                                                          aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcaga                                         >  2:4403546/1‑96 (MQ=37)
                                                           ttCACCCCCCGGNNNNTTTTNGCCAAGGTGAAATGNNAAAGGGC.GCTCCGCTGGG.GGCGCCNNTTCATCTTGCCACAAATANCCC.GGGG.TCC.gg.ggnngng                                        <  2:4431916/101‑7 (MQ=25)
                                                           ttCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCagag                                         <  2:449692/101‑1 (MQ=37)
                                                            tCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCG.GGGG.TCCGGG.GCAGAGc                                        >  2:4187627/1‑101 (MQ=37)
                                                             caagccccGGGCTATTTTCCCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.GGAGGCGCCCTTTCATCTTACCACAAATATCCA.GGGGCACA.GG.GCagaggc                                       >  2:2205407/5‑99 (MQ=255)
                                                             cACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGcc                                       >  1:99455/1‑101 (MQ=25)
                                                             cACCCCCCCGGATATTTACGCCAAGGGGAAATGGAACAGGGCGG.TCCGCTGGG.GGTGCCCTTTCATCTTGCCACAAATACCCCAGGGT.TCG.GG.GCagagtc                                       >  2:4311367/1‑99 (MQ=16)
                                                                cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCTCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.GGCGCAGAGCCCCg                                    <  1:3191990/101‑1 (MQ=25)
                                                                cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCg                                    <  1:2722538/101‑1 (MQ=25)
                                                                cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCAAAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCg                                    <  1:1479165/101‑1 (MQ=25)
                                                                 ccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCgg                                   <  1:1794191/101‑1 (MQ=25)
                                                                  cccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGc                                  >  1:4337457/1‑101 (MQ=25)
                                                                    cGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCt                                >  2:2712190/1‑101 (MQ=25)
                                                                    cGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCt                                >  2:1825226/1‑101 (MQ=25)
                                                                     gggATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCtt                               <  2:2297095/101‑1 (MQ=25)
                                                                     gggATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCtt                               >  2:1715491/1‑101 (MQ=25)
                                                                     gggATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCtt                               <  1:1188895/101‑1 (MQ=25)
                                                                     gggATATTTTCGCCAAGGGAAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTCCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCtt                               <  1:1654486/101‑1 (MQ=21)
                                                                       gATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGc                             >  2:2809059/1‑101 (MQ=25)
                                                                       gATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGc                             <  1:1630978/101‑1 (MQ=25)
                                                                        atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                            >  2:2318958/1‑101 (MQ=25)
                                                                        atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                            >  1:889909/1‑101 (MQ=25)
                                                                        atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                            <  2:1355850/101‑1 (MQ=25)
                                                                        atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                            >  2:4084490/1‑101 (MQ=25)
                                                                        atatTTTCGACAAGGAGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCACTTTCATCTTGCCACAAATATCTC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                            >  2:3872513/1‑101 (MQ=21)
                                                                         tatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTc                           <  2:3062915/101‑1 (MQ=25)
                                                                          atTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCa                          >  1:1491407/1‑101 (MQ=25)
                                                                          atTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCa                          <  2:1635578/101‑1 (MQ=25)
                                                                          atTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCa                          >  1:2839090/1‑101 (MQ=25)
                                                                           ttttCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAg                         >  2:2582815/1‑101 (MQ=25)
                                                                           ttttCGCCAAGGGGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAg                         >  1:2528582/1‑101 (MQ=25)
                                                                             ttCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGcc                       >  2:4016301/1‑101 (MQ=25)
                                                                              tCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCACCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGccc                      <  2:698708/101‑1 (MQ=25)
                                                                                 ccAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCaga                   >  1:2405804/1‑101 (MQ=25)
                                                                                 ccAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCaga                   <  1:4099492/101‑1 (MQ=25)
                                                                                    aGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                <  2:989474/101‑1 (MQ=25)
                                                                                     ggTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGaca               <  1:4135898/101‑1 (MQ=25)
                                                                                       tGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCGGCCCAGAGACAcc             >  1:4011442/1‑101 (MQ=25)
                                                                                        gAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCa            >  1:4533449/1‑101 (MQ=25)
                                                                                         aaaTGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg           >  1:1661807/1‑101 (MQ=25)
                                                                                           aTGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg         <  1:3496162/101‑1 (MQ=25)
                                                                                           aTGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg         >  2:335609/1‑101 (MQ=25)
                                                                                           aTGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg         <  1:3100090/101‑1 (MQ=25)
                                                                                            tGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc        >  2:892376/1‑101 (MQ=25)
                                                                                            tGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc        >  1:473928/1‑101 (MQ=25)
                                                                                            tGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc        <  2:896475/101‑1 (MQ=25)
                                                                                            tGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc        >  1:411108/1‑101 (MQ=25)
                                                                                             ggAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa       >  2:3397809/1‑101 (MQ=25)
                                                                                              gAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCAt      <  1:4156155/101‑1 (MQ=25)
                                                                                               aaaaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATg     >  2:2925177/1‑101 (MQ=25)
                                                                                               aaaaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATg     <  1:419243/101‑1 (MQ=25)
                                                                                                aaaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg    >  1:2674393/1‑101 (MQ=25)
                                                                                                aaaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg    >  1:3521290/1‑101 (MQ=25)
                                                                                                 aaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgc   <  2:1797267/101‑1 (MQ=25)
                                                                                                 aaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgc   <  1:4452636/101‑1 (MQ=25)
                                                                                                  aGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcg  >  1:191178/1‑101 (MQ=25)
                                                                                                         |                                                                                                  
AAGGCCACGATCGAGTGACACGGGCGCA.GGGG.GCTCTGCCCCCCCTCT.G.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.G.TCCGCT.GG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.GG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCG  >  NC_014034/1843321‑1843512

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG