Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,843,426 +G 100% intergenic (+88/+60) ppiB → / ← tyrS peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,843,4261.→G100.0% 501.5 154intergenic (+88/+60)ppiB/tyrSpeptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (91/63):  ref base (0/0):  total (91/63)

Only 100 of 170 total aligned reads displayed.

GATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.G.TCCGCT.GG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.GG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTTGC  >  NC_014034/1843329‑1843520
                                                                                                      |                                                                                                   
gATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCACCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.g                                                                                                    >  2:1599778/1‑101 (MQ=21)
 aTCGAGGGACACGGGCGGAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg                                                                                                   <  2:2547234/101‑1 (MQ=21)
  tCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.g                                                                                                 <  1:4039596/101‑1 (MQ=25)
     aGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggc                                                                                              <  1:3756269/101‑3 (MQ=21)
     aGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggc                                                                                              <  1:3708239/101‑3 (MQ=21)
     aGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggc                                                                                              <  2:3787784/101‑3 (MQ=21)
       tGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.gg.gggcgc                                                                                            <  2:4089810/101‑5 (MQ=21)
           acGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCtt                                                                                         <  1:3290981/101‑1 (MQ=21)
                cgcaggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATc                                                                                    <  2:4546926/96‑1 (MQ=21)
                   aggggggCTCTGCCCCCTCT.GC.TGCGCCTTCACCCCCCCCGCCATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGc                                                                                >  2:2043596/3‑101 (MQ=255)
                   aggggggCTCTG.CCCCCCTCTGCTGCGCATTCGCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTg                                                                                 <  2:1218018/99‑1 (MQ=21)
                     gggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGcc                                                                               >  1:3437789/1‑101 (MQ=21)
                          ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACaaa                                                                          >  1:54755/1‑101 (MQ=25)
                            tctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCCTCTTGCCACAAat                                                                         >  2:540449/5‑101 (MQ=25)
                            tctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAat                                                                         >  2:1630873/5‑101 (MQ=25)
                            tctgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAat                                                                         <  1:4551485/97‑1 (MQ=25)
                              tgccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAatat                                                                       >  2:2957751/3‑101 (MQ=25)
                               gccccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATc                                                                      >  1:2297257/2‑101 (MQ=37)
                                 cccccTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATccc                                                                    <  1:4233699/101‑1 (MQ=37)
                                 cccccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATccc                                                                    <  2:3979555/101‑1 (MQ=37)
                                   cccTCTGCTGCGCATTCACCCCCCGGGATATTTACGCCAAGGTGAAATGGAAAAGGGG.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.gg                                                                 <  1:3987956/101‑1 (MQ=37)
                                    ccTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAAGGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.ggg                                                                >  2:4030669/1‑101 (MQ=37)
                                     ctctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.gggg                                                               >  1:2965699/7‑101 (MQ=37)
                                      tctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.t                                                             >  1:4117413/6‑101 (MQ=38)
                                      tctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.t                                                             >  2:3099846/6‑101 (MQ=38)
                                       ctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tc                                                            >  2:1188803/5‑101 (MQ=38)
                                       ctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tc                                                            >  2:2892934/5‑101 (MQ=38)
                                       ctgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCA.GGGC.Tc                                                            >  1:768566/5‑101 (MQ=37)
                                        tgctgCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tcc                                                           <  1:1690701/98‑1 (MQ=37)
                                        tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tcc                                                           <  1:4466189/98‑1 (MQ=38)
                                        tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tcc                                                           >  1:1330472/4‑101 (MQ=38)
                                        tgctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.Tcc                                                           >  2:4162980/4‑101 (MQ=38)
                                         gctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.g                                                         >  2:1014258/3‑101 (MQ=38)
                                         gcggggcATTCTCCCCCAGGGATATTTTCGCCAAGGTGAAATGGAAAACGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.g                                                         <  2:1171744/96‑1 (MQ=25)
                                          ctgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg                                                        >  2:3616954/2‑101 (MQ=38)
                                           tgCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.g                                                      >  1:3895564/1‑101 (MQ=38)
                                            gcgcATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.gg                                                     <  2:2373885/101‑2 (MQ=38)
                                             cgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggc                                                    >  2:1731991/1‑99 (MQ=38)
                                             cgcATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggc                                                    <  1:2625026/101‑3 (MQ=38)
                                               cATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcag                                                  >  1:1635683/1‑97 (MQ=37)
                                                aTTCACCCCCGGTGATATTTTGGCCAAGGCGAAATGGCAAAAGGC.GCTCCGCT.GGAGGCGCCCTTTCATCTTGCCACAAATATCTA.GGGG.TCC.gg.gacaga                                                 >  2:2876788/1‑96 (MQ=19)
                                                aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcaga                                                 >  2:4403546/1‑96 (MQ=37)
                                                aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcaga                                                 >  2:2085309/1‑96 (MQ=37)
                                                aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcaga                                                 >  1:347602/1‑96 (MQ=37)
                                                aTTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.gg.ggcaga                                                 >  2:33448/1‑96 (MQ=37)
                                                 ttCACCCCCCGGNNNNTTTTNGCCAAGGTGAAATGNNAAAGGGC.GCTCCGCTGGG.GGCGCCNNTTCATCTTGCCACAAATANCCC.GGGG.TCC.gg.ggnngng                                                <  2:4431916/101‑7 (MQ=25)
                                                 ttCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCagag                                                 >  1:3670744/1‑101 (MQ=37)
                                                 ttCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCagag                                                 <  2:449692/101‑1 (MQ=37)
                                                  tCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCG.GGGG.TCCGGG.GCAGAGc                                                >  2:4187627/1‑101 (MQ=37)
                                                   caagccccGGGCTATTTTCCCCAAGGTGAAATGGAAAAGGGC.GCTCCGCT.GGAGGCGCCCTTTCATCTTACCACAAATATCCA.GGGGCACA.GG.GCagaggc                                               >  2:2205407/5‑99 (MQ=255)
                                                   cACCCCCCCGGATATTTACGCCAAGGGGAAATGGAACAGGGCGG.TCCGCTGGG.GGTGCCCTTTCATCTTGCCACAAATACCCCAGGGT.TCG.GG.GCagagtc                                               >  2:4311367/1‑99 (MQ=16)
                                                      cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCTCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.GGCGCAGAGCCCCg                                            <  1:3191990/101‑1 (MQ=25)
                                                      cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCTCg                                            <  1:3191991/101‑1 (MQ=25)
                                                      cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCg                                            <  1:2722538/101‑1 (MQ=25)
                                                      cccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCAAAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCg                                            <  1:1479165/101‑1 (MQ=25)
                                                       ccccGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCgg                                           <  1:1794191/101‑1 (MQ=25)
                                                          cGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCt                                        >  2:2712190/1‑101 (MQ=25)
                                                           gggATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCtt                                       <  2:2297095/101‑1 (MQ=25)
                                                           gggATATTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCtt                                       <  1:1188895/101‑1 (MQ=25)
                                                           gggATATTTTCGCCAAGGTGAAACGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.GCCGGG.GCAGCGCCAGGGCCtc                                       >  2:1338727/1‑100 (MQ=21)
                                                              atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                                    <  2:1355850/101‑1 (MQ=25)
                                                              atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                                    >  1:4140686/1‑101 (MQ=25)
                                                              atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                                    >  1:889909/1‑101 (MQ=25)
                                                              atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                                    >  2:2318958/1‑101 (MQ=25)
                                                              atatTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                                    >  2:4135610/1‑101 (MQ=25)
                                                              atatTTTCGACAAGGAGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCACTTTCATCTTGCCACAAATATCTC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCt                                    >  2:3872513/1‑101 (MQ=21)
                                                                atTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCa                                  >  1:3728510/1‑101 (MQ=25)
                                                                atTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCa                                  >  1:360182/1‑101 (MQ=25)
                                                                atTTTCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCa                                  >  1:2839090/1‑101 (MQ=25)
                                                                 ttttCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAg                                 >  2:2582815/1‑101 (MQ=25)
                                                                 ttttCGCCAAGGGGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAg                                 >  1:2528582/1‑101 (MQ=25)
                                                                   ttCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGcc                               >  2:2779174/1‑101 (MQ=25)
                                                                    tCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGccc                              <  2:710609/101‑1 (MQ=25)
                                                                    tCGCCAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCACCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGccc                              <  2:698708/101‑1 (MQ=25)
                                                                       ccAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCaga                           >  1:346009/1‑101 (MQ=25)
                                                                       ccAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCaga                           <  1:4099492/101‑1 (MQ=25)
                                                                       ccAAGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCaga                           >  1:2405804/1‑101 (MQ=25)
                                                                          aGGTGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGac                        <  2:989474/101‑1 (MQ=25)
                                                                             tGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc                     >  2:1446384/1‑101 (MQ=25)
                                                                             tGAAATGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACAcc                     >  2:1797421/1‑101 (MQ=25)
                                                                               aaaTGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAg                   >  1:1661807/1‑101 (MQ=25)
                                                                                 aTGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg                 <  1:3496162/101‑1 (MQ=25)
                                                                                 aTGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcg                 <  1:3100090/101‑1 (MQ=25)
                                                                                  tGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc                <  2:896475/101‑1 (MQ=25)
                                                                                  tGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc                <  1:1515488/101‑1 (MQ=25)
                                                                                  tGGAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAgcgc                >  1:473928/1‑101 (MQ=25)
                                                                                   ggAAAAGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCa               >  2:3397809/1‑101 (MQ=25)
                                                                                     aaaaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGacagatcggaaga             >  2:1759511/1‑91 (MQ=21)
                                                                                     aaaaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATg             <  1:419243/101‑1 (MQ=25)
                                                                                      aaaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg            >  1:2674393/1‑101 (MQ=25)
                                                                                      aaaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATgg            >  1:3521290/1‑101 (MQ=25)
                                                                                       aaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgc           <  1:4452636/101‑1 (MQ=25)
                                                                                       aaGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgc           >  2:1680467/1‑101 (MQ=25)
                                                                                        aGGGC.GCTCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcg          >  1:191178/1‑101 (MQ=25)
                                                                                          gggcgctCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcgc         <  1:4417877/95‑1 (MQ=25)
                                                                                          gggcgctCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGgcgc         >  1:1614810/7‑101 (MQ=25)
                                                                                              gctCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGcttct     >  2:4015431/3‑101 (MQ=37)
                                                                                               ctCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGTCACCAGCGCATGGCGcttctt    <  1:2640754/100‑1 (MQ=25)
                                                                                                tCCGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTTg   <  1:4084185/101‑1 (MQ=37)
                                                                                                 ccGCTGGG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCCGGG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTTGc  <  2:3437535/101‑1 (MQ=37)
                                                                                                      |                                                                                                   
GATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGC.G.TCCGCT.GG.GGCGCCCTTTCATCTTGCCACAAATATCCC.GGGG.TCC.GG.GCAGAGCCCCGGCCTTGCTCAGCCCAGAGACACCAGCGCATGGCGCTTCTTGC  >  NC_014034/1843329‑1843520

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG